Garuda - Garba Rujukan Digital

PENGHAMBATAN ACTINOMYCETES TERHADAP Erwinia carotovora Subsp. carotovora SECARA IN VITRO Sallytha, Ariestya Ayu Meda; Addy, Hardian Susilo; Mihardjo, Paniman Ashna
Berkala Ilmiah Pertanian Vol 1, No 4: MEI
Publisher : Berkala Ilmiah Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.239 KB)

[ENGLISH]The efforts to increase tobacco production is influence by the limiting factor in the field, such as plant disease. One of them is due to interference by Hollow Stalk disease caused by Erwinia carotovora subsp. carotovora. Some studies suggest that Actinomycetes can be used as a biological control for controlling Hollow Stalk on tobacco as it potentially produce antibiotics. This study was aimed to obtain isolates of Actinomycetes in the rhizospheric and soil in the area of tobacco plantation and to learn the inhibition of Actinomycetes against E. carotovora in vitro. The research was carried out with various stages, such as exploration and isolation of Actinomycetes, culturing E. carotovora, and testing of the inhibition in vitro through double-layer assay method. Exploration isolates of Actinomycetes resulted 12 isolates from four locations: Mumbulsari, Sukorambi, Sukowono and Gebang. Microbial colonies that grew white, not shiny with small diameter 3-20 mm was chosen for antibiotics test. Based on testing antibiosis against E. carotovora, all isolates Actinomycetes antagonistic properties showed formation of inhibition zone diameter of inhibition despite variations in individual isolates. Inhibition zone was vary with range of 18.30 mm to 49.95 mmof diameter that was shoing by isolate of Sukorambi 3 and Mumbulsari 2, respectively. The variation of the difference of differences of power allegedly inhibitory antagonism of each isolate Actinomycetes while also differences in produce antibiotics as inhibitors of the growth of pathogens.Keywords: Erwinia carotovora; Actinomycetes; Antibiotics[INDONESIAN]Usaha peningkatan produksi tembakau dipengaruhi adanya faktor pembatas di lapangan, seperti penyakit tumbuhan. Salah satu diantaranya adalah akibat gangguan penyakit busuk batang berlubang yang disebabkan oleh Erwinia carotovora subsp. carotovora. Beberapa penelitian menyatakan bahwa Actinomycetes dapat dimanfaatkan sebagai pengendali hayati untuk mengendalikan bakteri busuk batang berlubang pada tembakau karena berpotensi menghasilkan antibiotik. Penelitian ini bertujuan untuk mendapatkan isolat Actinomycetes pada daerah rizosfer dan tanah di pertanaman tembakau dan mengetahui daya hambat Actinomycetes terhadap E. carotovora secara in vitro. Penelitian ini dilakukan dengan berbagai tahapan, seperti eksplorasi danisolasi Actinomycetes, kultur E. carotovora, dan pengujian daya hambat secara in vitro dilakukan melalui pendekatan antibiosis dengan metode doublelayer assay. Isolat Actinomycetes hasil ekplorasi yang berhasil diisolasi sebanyak 12 isolat dari empat lokasi yaitu Mumbulsari, Sukorambi, Sukowono dan Gebang. Koloni mikrob yang tumbuh berwarna putih, tidak mengkilap dengan diameter kecil 3-20 mm. Berdasarkan pengujian antibiosis terhadap E. carotovora, semua isolat Actinomycetes memiliki sifat antagonis ditunjukkan dengan terbentuknya zona penghambatan meskipun terdapat variasi diameter penghambatan pada masing-masing isolat. Diameter zona hambatan yang terbentuk berkisar 18,30 mm yang ditunjukkan oleh isolat Actinomycetes asal Sukorambi 3 hingga 49,95 mm yang ditunjukkan oleh isolat Actinomycetes asal Mumbulsari dua. Adanya variasi perbedaan penghambatan diduga adanya perbedaan daya antagonisme dari masing-masing isolat Actinomycetes selain itu juga perbedaan dalam menghasilkan antibiotik sebagai penghambat pertumbuhan patogen.Kata Kunci: Erwinia carotovora; Actinomycetes; AntibiotikHow to citate: Sallytha AAM, HS Addy, PA Mihardjo. 2014. Penghambatan actinomycetes terhadap Erwinia carotovora subsp. carotovora secara in vitro. Berkala Ilmiah Pertanian 1(4): 70-72.

Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Restanto, Didik Pudji; ., Slameto; Kriswanto, Budi; Addy, Hardian Susilo; Handoyo, Tri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.Â It was greatly reduces the quality and production in the world such as India.Â The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTAÂ TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).Â The CBSD RNA concentration around 55,2ng/ul.Â The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extentionÂ (72oC for 1 min).Â The results showed a single band of about 380 bp which is theÂ distance between the two primers were tested.Â The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR

EKSPLORASI Bacillus spp., DARI PERAKARAN KUBIS SEBAGAI AGEN ANTAGONIS Xanthomonas campestris pv. campestris Agustina Wati, Fajar Dwi; Nurcahyanti, Suhartiningsih Dwi; Addy, Hardian Susilo
AGRITROP Vol 15, No 2 (2017): Agritrop : Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Isolasi Bacillus spp diambil dari tanah rhizosfer kubis sehat di oven dengan suhu 80âC selama 1 jam dan ditumbuhkan pada media YPGA.Sebanyak 24 isolat Bacillus spp merupakan gram positif dan hipersensitif bersifat negatif. Isolat tersebut dilakukan uji zona penghambatan terhadap Xanthomonas campestris pv. campestris, terdapat 12 isolat yang membentuk zona hambatan. Ke 12 isolat Bacillus spp tersebut memiliki perbedaan ukuran daam membentuk zona hambatan dan memiliki mekanisme penghambatan bakteriostatik, isolat tersebut masing-masing diambil 2 perwakilan untuk kategori zona hambatan besar, sedang dan kecil. Terdapat 6 isolat terpilih, kemudian dilakukan karakterisasi yang terdiri dari uji oksidase, katalase, dan hidrolisa pati dan memiliki kesamaan dalam hasil karakeristiknya.

DETECTION OF GENES RESISTANT TO BACTERIAL LEAF BLIGHT IN RICE CULTIVARS FROM SITUBONDO AND JEMBER, INDONESIA .,, Rasmiyana; Addy, Hardian Susilo; Narulita, Erlia
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 19 No. 2 (2019): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j.hptt.219127-134

Detection of genes resistant to bacterial leaf blight in rice cultivars from Situbondo and Jember, Indonesia. Xanthomonas oryzae pv. oryzae (Xoo), causing bacterial leaf blight, is a destructive pathogen that significantly affects rice production. The use of resistant varieties is the most effective and economical strategy to reduce the impact of the disease. This study aimed to analyze the genetic basis of resistance to Xoo in rice. The incidence and severity of bacterial leaf blight were assessed in the field through a diagonal random sampling method. PCR was used to detect resistant genes in rice with eight Xa-specific primers. The presence of Xa genes and environmental conditions were statistically analyzed to determine whether the disease incidence and disease severity were related to average temperature and rain intensity. The means for disease incidence and severity at the generative stage were higher than those at the vegetative stage. The 12 rice cultivars were categorized into four groups based on the presence of Xa gene sequences; most cultivars had Xa10 and Xa13, and one cultivar had five Xa genes. Disease incidence and severity were weakly correlated to the presence of resistance genes, as well as to environmental factors. Most rice cultivars from the Regencies of Jember and Situbondo carry Xa10 and Xa13 resistance genes.

Insidensi dan Keparahan Penyakit Penting Tanaman Padi di kabupaten Jember Masnilah, Rachmi; Wahyuni, Wiwiek Sri; N, Suhartiningsih Dwi; Majid, Abdul; Addy, Hardian Susilo; Wafa, Ali
AGRITROP Vol 18, No 1 (2020): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agritrop.v18i1.3103

Jember merupakan salah satu Kabupaten penghasil padi terbesar di jawa timur, Indonesia. Sayangnya berdasarkan Badan Pusat Data Statistik (BPS) pada tahun 2015-2016, produksi padi di Kabupaten Jember Menurun hingga 20.000 ton per tahun. Masalah hama dan penyakit menjadi penyebab utama turunnya produktifitas padi di Jember. Di ketahui tanaman padi di Jember dapat diserang oleh beberapa penyakit dalam kurun waktu yang sama dengan insidensi dan keparahan yang berbeda. Penelitian dilakukan guna mengetahui tingkat insidensi dan keparahan penyakit penting di beberapa kecamatan di Kabupaten Jember. Lokasi pengambilan data diperoleh berdasarkan metode pengacakan bertingkat dan dilakukan di musim kering. Sampel diambil dari empat desa per kecamatannya dan per desa diambil minimal empat titik lahan. Hasil menunjukkan Beberapa penyakit diketahui menyerang semua lokasi penyakit seperti Penyakit hawar bakteri (Kresek) dan Blast. Umumnya masing-masing kecamatan berbeda jenis penyakit yang menyerang. Sebagai contoh Tungro dan penyakit lain yang diebabkan oleh virus hanya menyerang pada beberapa lokasi dengan insidensi dan keparahan yang kecil. Data hasil penelitian ini dapat digunakan untuk dasar peramalan terjadinya epidemi penyakit di kemudian hari dan mempersiapkan metode pengendalian penyakit tanaman padi yang bijaksana pada musim yang sama.

Synergism of Lumbricus rubellus and Pseudomonas putida Pf-20 in Inducing Resistance to Cucumber Mosaic Virus WIWIEK SRI WAHYUNI; HARDIAN SUSILO ADDY; BUDI ARMAN; TRI CANDRA SETYOWATI
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

Both Lumbricus rubellus and Pseudomonas putida decompose soil organic matters. The population of P. putida Pf-20 increased if L. rubellus was introduced to the cucumber growth medium. The process of organic decomposition was much better if the medium was introduced with both L. rubellus and P. putida Pf-20, compared to the medium contained only either one of those organisms. The activity of L. rubellus may serve to provide nutrients for both the cucumber and P. putida. The role of P. putida to reduce disease severity was increased if L. rubellus was introduced to the growth medium. The synergism of these two organisms, reduced either the level of disease severity to CMV-48 and C/N ratio of medium, but increased the content of available phosphor and potassium. Key words: Lumbricus rubellus, Pseudomonas putida Pf-20, disease severity of CMV

Penggunaan Bakteriofag untuk Kit Detektor Patogen Hawar Bakteri Kedelai Nurul Rama Dhany; Hardian Susilo Addy; Wiwiek Sri Wahyuni
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial blight disease on soybean caused by Pseudomonas syringae pv. glycinea is an important factor causing yield loss in Indonesia. Bacteriophage isolated from irrigation water around the soybean field can be used as indicator for the presence of phytopathogenic bacteria. The objectives of this research was to obtain suitable composition of detector materials to detect P. syringae pv. glycinea using bacteriophage. Composition of detector kit contains of nutrient broth medium with 0.1% of bromothymol blue, 10 g talk and 1 g CMC which will be rubbed on to the detector paper and caused green colour development (pH ± 7) when the paper was dipped on to bacteria suspension, added by a drop of bacteriophage suspension and incubated for 24 hour, the colour will be changed. Yellow color indicated growth activity of P. syringae pv. glycinea where as blue colour indicated suppression of P. syringae pv. glycinea.

Distinguishing resistances of transgenic sugarcane generated from RNA interference and pathogen‐derived resistance approaches to combating sugarcane mosaic virus Weny Nailul Hidayati; Retnosari Apriasti; Hardian Susilo Addy; Bambang Sugiharto
Indonesian Journal of Biotechnology Vol 26, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.65256

Sugarcane mosaic virus (SCMV) is a causative agent that reduces growth and productivity in sugarcane. Pathogen‐derived resistance (PDR) and RNA interference (RNAi) are the most common approaches to generating resis‐ tance against plant viruses. Two types of transgenic sugarcane have been obtained by PDR and RNAi methods using a gene‐encoding coat protein (CP) of SCMV (SCMVCp). This research aimed to distinguish resistance of the two transgenic sugarcanes in combating SCMV through artificial viral inoculation. The experiment was conducted using transgenic sugar‐ cane lines validated by PCR analysis. Insertion of gene‐encoding CP in the transgenic lines was confirmed by amplification of 702 bp of DNA fragment of SCMVCp. After viral inoculation, mosaic symptoms appeared earlier, at 21 days post inoculation (dpi) in PDR transgenic lines, but was at 26 dpi in RNAi transgenic lines. Symptom observation showed that 77.8% and 50% of the inoculated plants developed mosaic symptoms in PDR and RNAi transgenic lines, respectively. RT‐PCR analysis revealed that the nuclear inclusion protein b (Nib) gene of SCMV was amplified in the symptomatic leaves in plants classified as susceptible lines. Immunoblot analysis confirmed presence of viral CP with a molecular size of 37 kDa in the susceptible lines. Collectively, these results indicated that the RNAi approach targeting the gene for CP effectively produces more resistance against the SCMV infection in transgenic sugarcane compared to the PDR approach.

PENGHAMBATAN ACTINOMYCETES TERHADAP Erwinia carotovora Subsp. carotovora SECARA IN VITRO Ariestya Ayu Meda Sallytha; Hardian Susilo Addy; Paniman Ashna Mihardjo
Berkala Ilmiah Pertanian Vol 1 No 4 (2014): MEI
Publisher : Jember University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (773.239 KB)

[ENGLISH]The efforts to increase tobacco production is influence by the limiting factor in the field, such as plant disease. One of them is due to interference by Hollow Stalk disease caused by Erwinia carotovora subsp. carotovora. Some studies suggest that Actinomycetes can be used as a biological control for controlling Hollow Stalk on tobacco as it potentially produce antibiotics. This study was aimed to obtain isolates of Actinomycetes in the rhizospheric and soil in the area of tobacco plantation and to learn the inhibition of Actinomycetes against E. carotovora in vitro. The research was carried out with various stages, such as exploration and isolation of Actinomycetes, culturing E. carotovora, and testing of the inhibition in vitro through double-layer assay method. Exploration isolates of Actinomycetes resulted 12 isolates from four locations: Mumbulsari, Sukorambi, Sukowono and Gebang. Microbial colonies that grew white, not shiny with small diameter 3-20 mm was chosen for antibiotics test. Based on testing antibiosis against E. carotovora, all isolates Actinomycetes antagonistic properties showed formation of inhibition zone diameter of inhibition despite variations in individual isolates. Inhibition zone was vary with range of 18.30 mm to 49.95 mmof diameter that was shoing by isolate of Sukorambi 3 and Mumbulsari 2, respectively. The variation of the difference of differences of power allegedly inhibitory antagonism of each isolate Actinomycetes while also differences in produce antibiotics as inhibitors of the growth of pathogens.Keywords: Erwinia carotovora; Actinomycetes; Antibiotics[INDONESIAN]Usaha peningkatan produksi tembakau dipengaruhi adanya faktor pembatas di lapangan, seperti penyakit tumbuhan. Salah satu diantaranya adalah akibat gangguan penyakit busuk batang berlubang yang disebabkan oleh Erwinia carotovora subsp. carotovora. Beberapa penelitian menyatakan bahwa Actinomycetes dapat dimanfaatkan sebagai pengendali hayati untuk mengendalikan bakteri busuk batang berlubang pada tembakau karena berpotensi menghasilkan antibiotik. Penelitian ini bertujuan untuk mendapatkan isolat Actinomycetes pada daerah rizosfer dan tanah di pertanaman tembakau dan mengetahui daya hambat Actinomycetes terhadap E. carotovora secara in vitro. Penelitian ini dilakukan dengan berbagai tahapan, seperti eksplorasi danisolasi Actinomycetes, kultur E. carotovora, dan pengujian daya hambat secara in vitro dilakukan melalui pendekatan antibiosis dengan metode doublelayer assay. Isolat Actinomycetes hasil ekplorasi yang berhasil diisolasi sebanyak 12 isolat dari empat lokasi yaitu Mumbulsari, Sukorambi, Sukowono dan Gebang. Koloni mikrob yang tumbuh berwarna putih, tidak mengkilap dengan diameter kecil 3-20 mm. Berdasarkan pengujian antibiosis terhadap E. carotovora, semua isolat Actinomycetes memiliki sifat antagonis ditunjukkan dengan terbentuknya zona penghambatan meskipun terdapat variasi diameter penghambatan pada masing-masing isolat. Diameter zona hambatan yang terbentuk berkisar 18,30 mm yang ditunjukkan oleh isolat Actinomycetes asal Sukorambi 3 hingga 49,95 mm yang ditunjukkan oleh isolat Actinomycetes asal Mumbulsari dua. Adanya variasi perbedaan penghambatan diduga adanya perbedaan daya antagonisme dari masing-masing isolat Actinomycetes selain itu juga perbedaan dalam menghasilkan antibiotik sebagai penghambat pertumbuhan patogen.Kata Kunci: Erwinia carotovora; Actinomycetes; AntibiotikHow to citate: Sallytha AAM, HS Addy, PA Mihardjo. 2014. Penghambatan actinomycetes terhadap Erwinia carotovora subsp. carotovora secara in vitro. Berkala Ilmiah Pertanian 1(4): 70-72.

Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Didik Pudji Restanto; Slameto .; Budi Kriswanto; Hardian Susilo Addy; Tri Handoyo
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers. It was greatly reduces the quality and production in the world such as India. The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid). The CBSD RNA concentration around 55,2ng/ul. The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention (72oC for 1 min). The results showed a single band of about 380 bp which is the distance between the two primers were tested. The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR

Title

Found 18 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 18 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 18 Documents
Search