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All Journal AL KAUNIYAH Jurnal Riset Kimia JURNAL BIOLOGI INDONESIA Makara Journal of Science
PINGKAN ADITIAWATI
Microbial Biotechnology Research Group, School of Life Sciences and Technology Institut Teknologi Bandung, Jl. Ganesha 10 Bandung, Jawa Barat 40132, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK Agustien, Anthoni; Nurhajati, Jetty; Udin, Linar Z.; Aditiawati, Pingkan
Jurnal Riset Kimia Vol 4, No 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.44

Abstract

 ABSTRAK Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18th hours of incubation and keratinase is produced at stationer phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg  Keywords: Protease alkaline, Keratinase, Thermofilic, Brevibacillus agri A-03   
Karakterisasi Produk Biosolubilisasi Lignit oleh Kapang Indigenus dari Tanah Pertambangan Batubara di Sumatera Selatan Sugoro, Irawan; Hermanto, Sandra; Sasongko, Dwiwahju; Indriani, Dea; Aditiawati, Pingkan
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.878 KB) | DOI: 10.14203/jbi.v7i2.3116

Abstract

ABSTRACTCharacterization of Lignite Biosolubilization Products by Indigenous Moulds from Soil ofCoal Mining in South Sumatera. Biosolubilization of coal is a potential technology of convertingsolid coal to liquid fuel and chemicals at ambient condition. Our previous research hassuccessfully isolated four moulds from soil at coal mining - South Sumatera and has potency aslignite biosolubilization agent, i.e. T1, T2, T4, T5. The objective of this research was to characterizeof lignite biosolubilization products by four isolates. The method used was sub-mergedculture. Cultivation medium was MSS+ (minimal salt + sucrose 0,1% + yeast extract 0,01% +lignite 5 %). Incubation was conducted at room temperature for 28 days. The result showed thatall indigenos moulds have different ability in lignite biosolubilization. The highestbiosolubilization occurred after 7 days of incubation belonging to T1 isolate. However, GC-MSanalysis showed the largest percentage of hydrocarbon compound which equivalent to gasolineand diesel was T5 after 7 days of incubation.Key words: Biosolubilization, lignite, moulds, coal.
Pemanfaatan Urea sebagai Sumber Nitrogen pada Biosolubisasi Batubara oleh Trichoderma sp. Mulyawati, Novi; Pikoli, Megga Ratnasari; Sugoro, Irawan; Aditiawati, Pingkan
Al-Kauniyah: Jurnal Biologi Vol 6, No 2 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.566 KB) | DOI: 10.15408/al-kauniyah.v6i2.2824

Abstract

Lignite coal was found abundant in Indonesia, but usage for this type of coal was still relatively low. Economic value of coal increases when it is solubilized. Biosolubilization of coal by utilize of microbes produces compounds equivalent to petroleum. In this research, effect of urea on lignite biosolubilization by Trichoderma sp. was examined. Method of this research consisted of spore inoculum preparation, biosolubilization lignite coal with a variety of treatment that consists of treatment A (MSS + sucrose  1% + coal 5% + urea), and treatment B (MSS + sucrose  1% + coal 5%). Results showed that the addition of urea supported lignit coal biosolubilization by Trichoderma sp. based on increase in medium pH, concentration of phenolic and conjugated aromatic compounds, and activity of extracellular enzyme. In addition, result of product characterization using GCMS revealed compounds equivalent to 13,60%, 26,20% and 90,8% respectively for gasoline, kerosene and diesel components. Those confirmed that urea can be used as an alternative nitrogen source to support Trichoderma sp. in lignit biosolubilization producing petroleum compounds.
KARAKTERISASI PRODUK BIOSOLUBILISASI LIGNIT OLEH KAPANG INDIGENUS DARI TANAH PERTAMBANGAN BATUBARA DI SUMATERA SELATAN Sugoro, Irawan; Hermanto, Sandra; Sasongko, Dwiwahju; Indriani, Dea; Aditiawati, Pingkan
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3116

Abstract

ABSTRACTCharacterization of Lignite Biosolubilization Products by Indigenous Moulds from Soil ofCoal Mining in South Sumatera. Biosolubilization of coal is a potential technology of convertingsolid coal to liquid fuel and chemicals at ambient condition. Our previous research hassuccessfully isolated four moulds from soil at coal mining - South Sumatera and has potency aslignite biosolubilization agent, i.e. T1, T2, T4, T5. The objective of this research was to characterizeof lignite biosolubilization products by four isolates. The method used was sub-mergedculture. Cultivation medium was MSS+ (minimal salt + sucrose 0,1% + yeast extract 0,01% +lignite 5 %). Incubation was conducted at room temperature for 28 days. The result showed thatall indigenos moulds have different ability in lignite biosolubilization. The highestbiosolubilization occurred after 7 days of incubation belonging to T1 isolate. However, GC-MSanalysis showed the largest percentage of hydrocarbon compound which equivalent to gasolineand diesel was T5 after 7 days of incubation.Key words: Biosolubilization, lignite, moulds, coal.
Isolation of Asphaltene-Degrading Bacteria from Sludge Oil Aditiawati, Pingkan; Kamarisima,
Makara Journal of Science Vol. 19, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sludge oil contains 30%–50% hydrocarbon fractions that comprise saturated fractions, aromatics, resins, and asphaltene. Asphaltene fraction is the most persistent fraction. In this research, the indigenous bacteria that can degrade asphaltene fractions from a sludge oil sample from Balikpapan that was isolated using BHMS medium (Bushnell-Hass Mineral Salt) with 0.01% (w/v) yeast extract, 2% (w/v) asphaltene extract, and 2% (w/v) sludge oil. The ability of the four isolates to degrade asphaltene fractions was conducted by the biodegradation asphaltene fractions test using liquid cultures in a BHMS medium with 0.01% (w/v) yeast extract and 2% (w/v) asphaltene extract as a carbon source. The parameters measured during the process of biodegradation of asphaltene fractions include the quantification of Total Petroleum Hydrocarbon (g), log total number of bacteria (CFU/ml), and pH. There are four bacteria (isolates 1, 2, 3, and 4) that have been characterized to degrade asphaltic fraction and have been identified as Bacillus sp. Lysinibacillus fusiformes, Acinetobacter sp., and Mycobacterium sp., respectively. The results showed that the highest ability to degrade asphaltene fractions is that of Bacillus sp. (isolate 1) and Lysinibacillus fusiformes (Isolate 2), with biodegradation percentages of asphaltene fractions being 50% and 55%, respectively, and growth rate at the exponential phase is 7.17x107 CFU/mL.days and 4.21x107 CFU/mL.days, respectively.
Co-Authors Anthoni Agustien Dadang Sumardi Dea Indriani Astuti Dwiwahju Sasongko Indriani, Dea Indriani, Dea Irawan Sugoro Kamarisima Kamarisima Kamarisima, Megga Ratnasari Pikoli, Megga Ratnasari Munawar . Novi Mulyawati, Novi Nurhajati, Jetty Rosmiati, Mia Sandra Hermanto Udin, Linar Z.