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All Journal Best Journal (Biology Education, Sains and Technology) Livestock and Animal Research
Mahbubillah, M. Ainul
Universitas Muhammadiyah Lamongan
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Public Health Veterinary

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Uji Aktivitas Antibakteri pada Ekstrak Etanolik Teripang Kering dari Ujung Pangkah Gresik M. Ainul Mahbubillah; Putri Ayu Ika Setiyowati; Muhammad Badrut Tamam; Fika Nur Fitriana Putri Bukhori
BEST Journal (Biology Education, Sains and Technology) Vol 5, No 1 (2022): Juni 2022
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v5i1.5233

Abstract

Sea cucumber is a potential fishery sub-sector, but its use still not popular because of its unfavorable physical form. However, from its unfavorable form, sea cucumbers contain many benefits as nutrition and medicine, including the presence of antimicrobial activity. The use of sea cucumbers as a nutrient is used by the people of Ujung Pangkah Gresik village as crackers. This study aims to prove the antimicrobial potential of dried sea cucumbers with crackers from Ujung Pangkah village, Gresik. The sea cucumbers have been boiled first before drying in the sun. The dried sea cucumbers were blended and then extracted using 96% ethanol. The extract was then sterilized by filtration using a 0.2 m PTFE filter. Extracts were tested using the Kirby-Bauer method on Mueller-Hinton Agar medium inoculated with Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Burkholderia cepacia, and yeast Candida albicans. The control antibiotic tetracycline 30 µg/ml and antifungal 2,5 µg/ml fluconazole were used as comparisons. The results of this study showed that all ethanolic extracts of dry sea cucumber with concentrations of 5%, 10%, and 15% did not show any clear zone. While the clear zone was shown in the control of tetracycline and fluconazole. This was caused by the loss of antimicrobial activity in the ethanolic extract of dried sea cucumbers derived from antimicrobial peptides which were damaged by heating during boiling process.
Inventarisasi Bryophyta, Pteridophyta, Gymnospermae Di Kabupaten Lamongan Dwi Zulianti; M. Ainul Mahbubillah; Nynda Ayu Nadira Savitri; Rofiatun Solekha
BEST Journal (Biology Education, Sains and Technology) Vol 4, No 1 (2021): Juni 2021
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v4i1.3596

Abstract

Indonesia has rich of plant species because of its habitat variation. Bryophyta, Pteridophyta, and Gymnospermae mostly grow on highland habitat. Height of the habitat causes change of micro-climate, especially humidity. Lamongan District of East Java Indonesia Jawa Timur is a lowland area so that difficult to find species of those three taxa. For educational purposes, data collection of the 3 taxa species that grow in Lamongan District is needed. The research was carried out by identifying plants Bryophyta, Pteridophyta, and in Lamongan District. The results of the study found that there were 3 Bryophyte species: Marchantia polymorpha, Polytrichum sp, and Riccia sp.; 3 Pteridophyte species: Pteridium aquilinum, Adiantum philippens, and Telmatoblechnum indicum; and 2 Gymnosperm species: Cycas rumphii, and Gnetum gnemon.   
Deteksi coronavirus pada kelelawar di Kabupaten Lamongan Muhammad Badrut Tamam; Aisyah Hadi Ramadani; M. Ainul Mahbubillah
Livestock and Animal Research Vol 20, No 1 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1027.802 KB) | DOI: 10.20961/lar.v20i1.56667

Abstract

Objective: To obtain information about the presence of coronavirus in bats to find potential of new disease reservoir as well as not yet reported disease reservoir in Lamongan District. This research can be used by the government as a basis for planning and evaluating disease control programs and for researchers it can be used for vaccine and drug design, viral phylogenetic, analysis of viral distribution, and viral databases.Methods: Sampling was carried out at 3 points representing different habitat types. The first point is at Pucakwangi Cave Babat Lamongan, the second is at Paciran Lamongan beach area, and the last is at the Mantup Forest Lamongan. Sampling was carried out using a mist net with the help of local workers. The netted bats were caught and put in a bag. Parameters observed included gender, morphometry, species, and detection of the presence of coronavirus. Detection of the virus using the oropharyngeal swab method, then the RNA samples were tested by RT-PCR and sequencing. The primer used to detect the coronavirus is primer forward 5′-CACGCAACTTGTTGTAATGCGT CAGAGA-3′ and primer reverse 5′- CACGTGCTTTTGCAGGCACTATACGAC-3′.Results: Five species of bats obtained from 3 sampling locations namely Taphozous melanopogon, Chaerophon sp., Hipposideros sp., Cynopterus sp., Macroglossus sp. RT-PCR test of sample G1, G6, G8, P1, P2, P3, H1, H3, and H6, shows that no coronavirus from DNA sample amplified.Conclusions: Negative result of Coronavirus detection by molecular analysis is not an indicator of no potential of species Taphozous melanopogon, Chaerophon sp., Hipposideros sp., Cynopterus sp., and Macroglossus sp. as a Coronavirus reservoir. Increasing the number of samples and expansion of study area still needed to obtain more comprehensive data.
Co-Authors Aisyah Hadi Ramadani Dwi Zulianti Fika Nur Fitriana Putri Bukhori Muhammad Badrut Tamam Muhammad Badrut Tamam Nynda Ayu Nadira Savitri Putri Ayu Ika Setiyowati Rofiatun Solekha