Claim Missing Document
Check
Articles

Found 35 Documents
Search

3-ACETYL -2,5,7-TRIHYDROXY-1,4-NAPHTALENEDIONE, AN ANTIMICROBIAL METABOLITE FROM THE CULTURE OF ENDOPHYTIC FUNGUS COELOMYCETES TCBP4 FROM Tinospora crispa Praptiwi, Praptiwi; Jamal, Yuliasri; Fathoni, Ahmad; Nurkanto, Arif; Agusta, Andria
Media Penelitian dan Pengembangan Kesehatan Vol 23, No 3 Sep (2013)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.471 KB)

Abstract

Abstract Isolation, identification and testing of antimicrobial activity of secondary metabolites of endophytic fungal culture TCBP4 isolated from bratawali (Tinospora crispa) has been performed. The fungus TCBP4 was cultivated in Potato Dextrose Broth (PDB) for 1 month, media and fungi extracted with ethyl acetate. The extract was separated and purified by several chromatographic techniques, from which 9 fractions were obtained. Fraction 3e was purified again and was obtained 6 fractions (3e1-3e6). Fractions 3e3-3e6 were tested against bacteria isolates Staphylococcus aureus Bacillus subtilis,Eschericia coli and yeast isolate Candida albicans by microdillution method. Antimicrobial activity test result showed that fractions 3e3-3e5 had better antibacterial activity compared to chloramphenicol as commercial antibiotic. It was indicated by MIC value of the fractions was lower (8 ug/ml) compared with the antibiotic chloramphenicol (16 ug/ml). Fraction 3e3 had better antifungal activity compared to commercial antifungal nystatin and cabisidin against C. albicans. GC-MS analysis showed that the chemical constituent of 3e5 fraction was identified as 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Key words : Tinospora crispa, endophytic fungi, isolation, identification, antimicrobial Abstrak Isolasi, identifikasi dan uji aktivitas antimikrobia terhadap metabolit sekunder dari kultur jamur endofit TCBP4 yang diisolasi dari tumbuhan bratawali (Tinospora crispa). Jamur endofit TCBP4 dikultivasi pada media Potato Dextrose Broth (PDB) selama 1 bulan, selanjutnya media dan jamur diekstrak dengan pelarut etil asetat. Ekstrak dipisahkan dan dimurnikan dengan beberapa teknik kromatografi sehingga diperoleh 9 fraksi. Fraksi 3e dimurnikan kembali dan diperoleh 6 fraksi (3e1-3e6). Fraksi 3e3-3e6 diuji aktivitas antibakterinya terhadap beberapa isolate bakteri Staphylo-coccus aureus Bacillus subtilis, Eschericia coli dan isolate khamir Candida albicans dengan metode mikrodilusi. Hasil uji antimikrobia menunjukkan bahwa fraksi 3e3-3e5 mempunyai aktivitas antibakteri lebih baik bila dibandingkan dengan chloramfenikol, dimana nilai MIC dari fraksi (8 ug/ml) lebih rendah dari antibiotika chloramfenikol (16 ug/ml). Fraksi 3e3 mempunyai aktivitas antijamur lebih baik bila dibandingkan dengan antijamur komersial nistatin dan kabisidin terhadap  C. albicans. Analisis GC-MS menunjukkan bahwa komponen kimia dari fraksi 3e5 diidentifikasi sebagai 3-acetyl -2,5,7-trihydroxy-1,4-naphtalenedione . Kata kunci : Tinospora crispa, jamur endofit, isolasi, identifikasi, antimikrobia
CHEMICAL CONSTITUENTS AND ANTIBACTERIAL EFFECT OF ESSENTIAL OIL OF JAVANEESE PEPPER LEAVES (PIPER RETROFRACTUM VAHL.) Jamal, Yuliasri; Irawati, Pipit; Fathoni, Ahmad; Agusta, Andria
Media Penelitian dan Pengembangan Kesehatan Vol 23, No 2 Jun (2013)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.498 KB)

Abstract

Abstrak Daun cabe jawa (Piper retrofractum Vahl.) telah terbukti berkhasiat sebagai bahan antimikroba, akan tetapi belum ada informasi lebih lanjut tentang mekanisme aksi antibakteri dari minyak atsiri daun cabe jawa. Penelitian ini untuk mengetahui pengaruh minyak atsiri daun cabe jawa terhadap bakteri uji sensitif serta difokuskan untuk melihat kerusakan membran sel bakteri melalui analisis protein, asam nukleat dan ion-ion logam kalsium dan kalium. Distilasi uap minyak atsiri dari daun segar cabe jawa (Piper retrofractum Vahl.) menghasilkan 0.03% cairan kental kekuningan. Analisis dengan GC-MS mengidentifikasi 4 senyawa sebagai komponen utama, yaitu germakren D (24.20%), tetrametilsiklo[5.3.1.0(4.11)]-undek-8-ena (17.73%), ar-turmeron (11.55%) dan benzil benzoat (6.28%). Minyak atsiri cabe jawa terbukti aktif melawan beberapa bakteri patogen antara lain Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus dengan diameter daya hambat berturut-turut 8.0; 9.7; 8.5 mm. Pengamatan lebih lanjut memperlihatkan nilai MIC dari minyak atsiri cabe jawa  terhadap B. subtilis adalah 2% (v/v), dan juga merubah morfologi sel dari bakteri yang diobservasi menggunakan SEM. Kata kunci: Piper retrofractum Vah., cabe jawa, minyak atsiri, aktivitas antibakteri Abstract The leaves of Javaneese pepper (Piper retrofractum Vahl.) has been proven efficacious as antimicrobial agent, however, there was no further information about action mechanism of antibacterial of Javaneese pepper leaves essential oil. This study was to determine the effect of essential oils of javaneese pepper leaves against bacterial sensitive test and is focused on investigating the damage of cell membranes of bacteria through the analysis of proteins, nucleic acids and metal ions, calcium and potassium. Steam distillation of essential oil from fresh leaves of Javaneese pepper (Piper retrofractum Vahl.) gave 0.03 % yellowish viscous liquid. Germacrene D (24.20 %), tetramethylcyclo[5.3.1.0(4.11)]-undec-8-ene (17.73 %), Ar-turmeron (11.55 %) and benzyl benzoate (6.28 %) were identified as major constituents analyzed by GC-MS. Javaneese pepper essential oil was active against pathogenic bacteria Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus with diameter of clear zone of 8.0, 9.7 and 8.5 mm respectively. Further investigation showed MIC value of the oil against B. subtilis was 2 % (v/v), and was also alter the cell morphology of tested bacterium observed by SEM. Key words: Piper retrofractum Vahl., Javeneese pepper, essential oil, antibacterial activity
SKRINING AWAL AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SEMUT (INSECTA: FORMICIDAE) DARI GARUT - JAWA BARAT Efendy, Oscar; Fathoni, Ahmad; Praptiwi, Praptiwi; Royyani, Mohammad Fathi; Wulansari, Dewi; Agusta, Andria
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3621

Abstract

Studies on the therapeutic use of insects and insect products have been neglected compared to the use of other animals or plants.This study aims to determine the antibacterial and antioxidant potential of ants extracts. Preliminary study related to antibacterial and antioxidant screeningof 17 extracts of ant colonies that belongs to 8 species were performed by Thin Layer Chromatography (TLC)-Bioautography. Antioxidant activity was measured by DPPH free radical scavenging method. The antibacterial activity was done against three pathogenic bacteria, i.e Bacillus subtilis InaCC B-1, Staphylococcus aureus InaCC B-4 and Escherichia coli InaCC B-5 were performed by non-eluted TLC-autobiography assay. The minimum inhibitory concentration (MIC) and IC50 values of DPPH radical scavenging activity of active extracts were determined by microdilution in 96-well microplate. The results showed 6 extracts active against B.subtilis, 5 extracts active against S.aureus. The lowest MIC value was 512 µg/ ml.  Ten extracts had the antioxidant activity with various IC50 values. The extracts of ants might be used as bioactive resources for antibacterial and antioxidant.  
KOMPOSISI MINYAK ATSIRI DARI TIGA JENIS TUMBUHAN RUTACEAE [ Composition of Essential Oils from Three Rutaceae Species Plant] Agusta, Andria; Jamal, Yuliasri
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.358 KB) | DOI: 10.14203/beritabiologi.v4i5.1251

Abstract

Rutaceae is one of the essential oil resources that is very potential for medicinal, perfumery and other use This study was conducted to analyze the essential oil components resulted from water distillation of kalamanjarik leaves (Micromelum minutum), jeruk epara leaves ILimnocitrus litoralis) and the leaves and the stem bark of malapotung (Evodia qlabra). The results showed the leaves of kalamanjarik contained approximately 0,70%, jeruk jepara 0,21%, malapotung leaves 0,17% and the stem bark contained 0,50 % essential oils. GCMS analyses of the essential oils gave 3 major components of kalamanjarik leaves i.e /J-pinene (24,41%j, bergamiol (23,01%) and nerolidol (18,90%) and 2 major components of jeruk jepara leaves i.e fl-mircene (70.55) and /t-pinene (24,06 %). Essential oil of malapotung leaves consisted of 39,8J% ct-pinene, 14,26 % 1aR(1aa,4a/3,7a,7aa,7ba)decahydro -1,1,7-lrimethyl-4-methylene-1H-cycloprope azulene and 16,62% S-cadinene as major components, while the stem bark had only one major component, u-pinene (68,37 %) The minor components of the four essential oils will be discussed.
ANALISIS KOMPONEN KIMIA DAUN WATI {PIPER METHYSTICUM FORST. F) Agusta, Andria; Jamal, Yuliasri; Chairul, Chairul
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1276

Abstract

Wati(Piper methysticum Forst.f.)leaves has been used as narcotic in some parts of Irian Jaya since longtime ago. The GCMS analysis of wati leaves organic extracts(hexane,chloroform, methanol)has detected 48 components consisted alkanes,oxygenated hydrocarbons, fatty acid, steroids and alkaloids,including 5 major components, i.e. dihydro kawain,anisol p-undecyl, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-on, levulinic acid and one of unknown component.Forty-three other minor components will be discussed.
Produksi Metabolit Utama (-)-Citrinin, pada Kultur Jamur Endofit Penicillium sp dari Tanaman Teh Agusta, Andria; Jamal, Yuliasri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.756 KB) | DOI: 10.24002/biota.v13i3.2570

Abstract

Endophytic fungi have been recognized as source of broad range biological active metabolites with high chemical structure diversity. The purpose of this research is to isolate and characterize major metabolite produce by the endophytic fungi Penicillium sp isolated from a tea plant. Cultivation of endophytic fungus Penicillium sp (AB2245443) in liquid medium PDB on a rotary shaker at 100 rpm, temperature 25 – 30oC for 7 days, produced a yellow metabolite. Separation of metabolite through chromatography technique and followed by chemical structure elucidation based on MS, IR, NMR spectra and published data showed that the yellow metabolite is (-)-citrinin.
()-(2 R,3S)-DIHIDROKUERSETIN, SUATU PRODUK BIOTRANSFORMASI (-)-EPIKATEKIN OLEH JAMUR ENDOFIT Diaporthe sp. E Agusta, Andria
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (802.608 KB) | DOI: 10.14203/beritabiologi.v9i4.2009

Abstract

The endophytic fungus Diaporthe sp.E show a unique ability to biotransform (-)-epicatechin into (-)-(2R,3R,4R)-leucocyanidin in a semisynthetic medium. Extension of the incubation time gave a major product (-)-(2R,35)-dihydroquercetin which was identified by spectroscopic methods.
The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Sumiasih, Inanpi Hidayati; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
International Journal of Applied Biology Vol 1, No 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
Eksplorasi Keanekaragaman Aktinomisetes Tanah Ternate Sebagai Sumber Antibiotik Nurkanto, Arif; Listyaningsih, Febrianti; Julistiono, Heddy; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.268 KB) | DOI: 10.14203/jbi.v6i3.3141

Abstract

ABSTRACTExploration of Soil Actinomycetes Diversity from Ternate as Indigenous Antibiotic Sources.Actinomycetes of soil samples from Ternate, North Moluccas were isolated using SDS-YEmethod in humic acid vitamin agar. Ternate has high abundance of Actinomycetes, approximately6.00 – 487 x 104 CFU/ g soil, depends on habitat types. We have selected 60 isolates andconducted antibiotic screening against pathogenic bacteria and fungi using agar diffusionmethod and found both narrow and broad antibiotic spectrum types . Based on 16S rDNAanalysis, all Actinomycetes with antibiotic activities are belong to the genus Streptomyces. .Minimum Inhibitor Concentration (MIC) value was determined by broth microdilution method.It was found that MIC values varied, depended on microbial tested. We found two isolateswith higher antibiotic activity compared to the commercial antibiotics (chloramphenicol,erythromycin for antibacterial and nystatin, kabicidin for antifungal). Cell destruction analysiscaused antibiotic activities was conducted through leak of protein and nuclatic acid.Key words : Actinomycetes, soil, Ternate, antibiotic, cell distruction
Identifikasi Molekular dan Karakterisasi Morfo-Fisiologi Actinomycetes Penghasil Senyawa Antimikroba Nurkanto, Arif; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2193

Abstract

ABSTRACTThe objectives of study were to identify antimicrobial producing Actinomycetes using 16S rDNA analyses and morphology and physiology characteristics. Eight Actinomycetes strain with the higest antibacterial and antifungal activity were selected and identified using six primers (20F, 520F, 920F, 1500R, 920R, and 520R). Morphological observation and physiology analyses were performed to the selected strain to accurately identify the strains. Morphological characters observed were aerial mycelium, spore chain, colony form, and pigment production. Physiological characterizations were antimicrobial properties, growth temperature, pH tolerance, salinity concentration for growth, sugars assimilation, and some enzymes production (arginine dihydrolase, urease, ß-glucosidase, protease, ß-galactosidase). Based on homology search by BLAST program and phylogenetic tree analyses, all of isolates were identified as the genus Streptomyces. They belong to eight different spesies. Isolates RC-SS-37-4, RC-SS-37-16 and BL-22-3 have been identified as Streptomyces costaricanus (100 %), Streptomyces costaricanus (99.8 %) and Streptomyces parvulus (98.6 %), respectively. Five isolates were identified as Streptomyces spp. (BL-36-1, BL-20-2, BL-14-2, BL-22-1 and BL-06-5) and can be presumed as new species because of the low homology value to their closest related spesies. Keywords : actinomycetes, antimicrobial, morphology, phylogenetic, physiology, 16S rRNA gene.