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PERTUMBUHAN VEGETATIF TANAMAN TOMAT (LYCOPERSICUM ESCULENTUM MILL.) DARI BENIH LAMA YANG DIINDUKSI KUAT MEDAN MAGNET 0,1 MT, 0,2 MT, DAN 0,3 MT Novitasari, Vina; Agustrina, Rochmah; Irawan, Bambang; Yulianty, Yulianty
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3816

Abstract

ABSTRACTTomatoes (Lycopersicum esculentum Mill.) horticultural which are very good for consumption as well as industrial materials.  However, the cultivation of tomatoes still faces many obstacles, one of them is the seed.  The quality of old seeds decreases with age of seeds, so that it will affect a crop production.  This study aims to determine whether the magnetic field strength can improve tomato plant vigor.  The study was conducted using a completely randomized design (CRD) of one factor, the induction of a magnetic field consisting of 3 levels, namely 0.1 mT (M0.1), 0.2 mT (M0.2), 0.3 mT (M0,3) for 7 minutes 48 seconds. This study uses two controls;  positive control the new seed (Sn) and negative control was the old seed (So) from not being given a magnetic field treatment each experiment unit is repeated 5 times.  The parameters measured were plant height, chlorophyll content, and carbohydrate content.  The data obtained were analyzed.  If there was a difference between treatments, it was continued with the smallest difference between treatments using the Tukey's test at the 5% level.  The results of the analysis prove that the magnetic field induction of the old seed can increase seed vigor, causing plant height, chlorophyll and carbohydrate content to be the same as plants from new seeds.    Keywords: tomatoes, old seeds, strong magnetic field  
OPTIMASI MEDIA PRODUKSI ENZIM XILANASE DARI Bacillus sp. (Medium Optimization of Xylanase Production from Bacillus sp.) Erika, Erika; Agustrina, Rochmah; Sumardi, Sumardi; Mulyono, Mulyono
JURNAL SELULOSA Vol 6, No 01 (2016): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.035 KB) | DOI: 10.25269/jsel.v6i01.72

Abstract

Xylan is a carbon source in growth medium of extracellular xylanase producing bacteria. The purpose of this study was to get the optimum medium for the growth of Bacillus sp. in producing the xylanase. The factors consist of production time, carbon, and nitrogen source, as well as simple sugars. Addition carbon source used was delignified sugarcane bagasse, rice hulls, and corn cobs with different concentrations (0.25%; 0.5%; 0.75%; and 1% w/v) . Ammonium chloride, ammonium sulfate, and sodium nitrate with different concentrations (0.08%; 0.17%; 0.26%; and 0.35% w/v) were used as a source of nitrogen, while the simple sugar used was glucose, lactose, sucrose, and xylose. The results showed that the optimum culture media of Bacillus sp. to produce xylanase is media with 0.25% natural starch from the corn cob xylan as a carbon source, 0.26% ammonium chloride as a source of nitrogen, 0.0625 grams of sugar xylose, at pH 6, incubation temperature of 40°C, and 12 hours production time. In that media, xylanase activity was 0.2 U/mL.Keywords: agricultural waste, medium optimization, xylanase, Bacillus sp.   ABSTRAKXilan merupakan sumber karbon pada media pertumbuhan bakteri penghasil enzim ekstraseluler xilanase. Tujuan penelitian ini adalah mendapatkan media optimum untuk pertumbuhan Bacillus sp. dalam memproduksi xilanase. Perlakuan percobaan terdiri dari waktu produksi, sumber karbon, sumber nitrogen, dan penambahan gula sederhana. Sumber karbon yang digunakan adalah bagas tebu, sekam padi, dan tongkol jagung dengan variasi konsentrasi 0,25%; 0,5%; 0,75%; dan 1% (b/v) . Amonium klorida, amonium sulfat, dan natrium nitrat dengan variasi konsentrasi 0,08%; 0,17%; 0,26%; dan 0,35% (b/v) digunakan sebagai sumber nitrogen, sedangkan gula sederhana yang digunakan adalah glukosa, laktosa, sukrosa, dan xilosa masing-masing sebanyak 0,0625 b/v. Hasil percobaan menunjukkan bahwa media optimum pertumbuhan Bacillus sp. untuk produksi xilanase adalah media dengan 0,25% tepung xilan dari tongkol jagung sebagai sumber karbon, 0,26% amonium klorida sebagai sumber nitrogen, 0,0625 gram gula xilosa, pada pH media 6, suhu inkubasi 40°C, serta waktu produksi 12 jam. Dalam media tersebut, aktivitas xilanase yang dihasilkan sebesar 0,2 U/mL.Kata kunci : limbah pertanian, optimasi media, xilanase, Bacillus sp. 
ISOLASI DAN KARAKTERISASI AKTIVITAS ENZIM ALPHA-AMILASE PADA KECAMBAH KEDELAI PUTIH (Glycine max (L). Merill) DAN KACANG HIJAU (Phaseolus radiatus) DI BAWAH PENGARUH MEDAN MAGNET Angraini, Widia; Sumardi, Sumardi; Handayani, Tundjung Tripeni; Agustrina, Rochmah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati Vol. 1 No. 1 (2013)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v1i1.147

Abstract

Kedelai putih (Glycine max (L.) Merill) dan kacang hijau (Phaseolus radiatus) banyak dimanfaatkan sebagai bahan pangan karena kandungan protein dan karbohidratnya sangat tinggi. Saat berkecambah, karbohidrat dalam biji diurai menjadi senyawa yang lebih sederhana oleh enzim alpha-amilase. Salah satu faktor lingkungan yang mempengaruhi perkecambahan biji adalah medan magnet karena dapat mempercepat perkecambahan, sintesis protein, dan aktivitas di dalam sel. Tujuan penelitian ini adalah mengisolasi dan mengetahui karakter enzim alpha-amilase pada perkecambahan biji kedelai putih dan kacang hijau yang diberi perlakuan medan magnet. Penelitian dilaksanakan di Laboratorium Botani dan Laboratorium Biomolekuler Jurusan Biologi FMIPA Universitas Lampung dari bulan Mei sampai Juli 2012. Percobaan dilaksanakan menggunakan Rancangan Kelompok Teracak Lengkap dengan 3 ulangan. Perlakuan yang diberikan adalah lama pemaparan medan magnet 0,1 mT terdiri dari: 0 menit (kontrol), 7’48’’, 11’44’’ dan 15’36’’. Parameter yang diukur adalah aktivitas enzim alpha-amilase pada hipokotil dan kotiledon kecambah pada umur yang berbeda. Perbedaaan aktivitas enzim alpha-Amilase dianalisis berdasarkan rata-rata aktivitas enzim pada setiap perlakuan. Hasil penelitian menunjukkan bahwa medan magnet mempengaruhi aktivitas alpha-amilase selama perkecambahan. Lama pemaparan medan magnet yang baik untuk meningkatkan aktivitas alpha-amilase dan mempercepat perkecambahan kedelai putih adalah 15’36’’sedangkan untuk kacang hijau adalah 11’44’’ dan 15’36’’.
TYPES AND ACTIVITIES OF DAILY INSECT POLINATORS IN ORDER GARDEN AND GARDEN FRUIT LIWA Febriyanti, Devy; ROSA, EMANTIS; Agustrina, Rochmah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati Vol. 7 No. 2 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i2.148

Abstract

Pollinator insects are insects that play a role in pollinating plants. This research was conducted in December-January 2020 at the Liwa Botanical Garden which aims to determine the types and daily activities of pollinator insects in the Liwa Botanical Garden Ornamental and Fruit Parks. The research used observation methods in 2 locations, namely Ornamental Gardens and Fruit Gardens. Pollinator insect sampling using a sweap net. The data obtained were analyzed descriptively. The results found that including pollinator insects in the Ornamental Park were 11 species and 165 individuals, namely (Junonia orithya, Hypolimnas bolina bolina, Neptis clinioides gunongensis, Oriens gola, Euploea mulciber, Elymnias panthera, Apis cerana, Apis florea, Xilocopa confuse, Lucilia sericerata, Coccinella septempuncata), in the Fruit Garden there were 6 species and 276 individuals, namely (Bactrocera dorsalis, Eurema blanda, Zizina otis, Ypthima baldus newboldi, Apis cerana, and Apis florea). Observation of Junonia orithya's daily activities starts in the morning at 08: 00-11: 00 WIB. Flying activities are initiated by the butterfly around the flower plant then perching on the leaves to sunbathe, suck nectar, suck minerals, suck nectar repeatedly, and look for a partner to carry out mating activities. Observation of daily afternoon activities was carried out at 14: 00-16: 00 WIB Junonia orithya was seen carrying out her activities such as in the morning. When the brightness starts to decrease for example due to rain, the butterflies will rest and take shelter under the leaves or bush environment.
KETAHANAN TANAMAN TERONG (Solanum melongena L.) HASIL INDUKSI POLIPLOIDISASI DENGAN EKSTRAK UMBI KEMBANG SUNGSANG (Gloriosa superba L.) TERHADAP JAMUR Colletotrichum capsici (Syd.) Butler & Bisby Sari, Mulya; Ernawiati, Eti; Agustrina, Rochmah; Yulianty, Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati Vol. 1 No. 2 (2013)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v1i2.146

Abstract

Tujuan penelitian ini adalah memperoleh konsentrasi ekstrak umbi kembang sungsang (Gloriosa superba) yang optimum untuk menghambat perkembangan jamur Colietotrichum capsici dan memperoleh kultivar tanaman terong (Solanum melongena) yang tahan terhadap penyakit antraknosa. Penelitian dilakukan di Laboratorium Botani, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung menggunakan Rancangan Acak Kelompok (RAK) yang terdiri dari 2 faktor dan 3 ulangan. Faktor pertama, konsentrasi ekstrak (A): 25%, 50%, dan 75%. Faktor kedua, kultivar terong (B): Wulung, Pahala dan Patria. Parameter pengamatan meliputi persentase kerusakan daun, tinggi tanaman, berat basah dan berat kering tanaman terong. Hasil percobaan menunjukkan bahwa ekstrak umbi kembang sungsang berpengaruh terhadap persentase kerusakan daun, tinggi tanaman, berat basah dan berat kering tanaman terong. Secara keseluruhan kombinasi perlakuan yang optimum untuk menekan perkembangan jamur C. capsici adalah konsentrasi 50% pada kultivar Wulung.
PERTUMBUHAN VEGETATIF TANAMAN TOMAT (LYCOPERSICUM ESCULENTUM MILL.) DARI BENIH LAMA YANG DIINDUKSI KUAT MEDAN MAGNET 0,1 MT, 0,2 MT, DAN 0,3 MT Novitasari, Vina; Agustrina, Rochmah; Irawan, Bambang; Yulianty, Yulianty
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3816

Abstract

ABSTRACTTomatoes (Lycopersicum esculentum Mill.) horticultural which are very good for consumption as well as industrial materials.  However, the cultivation of tomatoes still faces many obstacles, one of them is the seed.  The quality of old seeds decreases with age of seeds, so that it will affect a crop production.  This study aims to determine whether the magnetic field strength can improve tomato plant vigor.  The study was conducted using a completely randomized design (CRD) of one factor, the induction of a magnetic field consisting of 3 levels, namely 0.1 mT (M0.1), 0.2 mT (M0.2), 0.3 mT (M0,3) for 7 minutes 48 seconds. This study uses two controls;  positive control the new seed (Sn) and negative control was the old seed (So) from not being given a magnetic field treatment each experiment unit is repeated 5 times.  The parameters measured were plant height, chlorophyll content, and carbohydrate content.  The data obtained were analyzed.  If there was a difference between treatments, it was continued with the smallest difference between treatments using the Tukey's test at the 5% level.  The results of the analysis prove that the magnetic field induction of the old seed can increase seed vigor, causing plant height, chlorophyll and carbohydrate content to be the same as plants from new seeds.    Keywords: tomatoes, old seeds, strong magnetic field  
KAJIAN STRUKTUR ANATOMI DAN MORFOLOGI DAUN PLANLET PISANG KEPOK KUNING HASIL PEMBERIAN EKSTRAK UMBI KEMBANG SUNGSANG SECARA IN VITRO Saputra, Yoga Aji; Ernawiati, Eti; Agustrina, Rochmah; Wahyuningsih, Sri
Jurnal Biosilampari : Jurnal Biologi Vol 3 No 2 (2021): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v3i2.1268

Abstract

Plantain is a fruit commodity that has a high level of market demand, but the yield is low. To overcome this problem, tissue culture techniques are used to increase plantlet production in a short time. Plantain have parthenocarpy character, high sterility, and different levels of ploidy. Tissue culture with the addition of flame lily tuber biomutagen containing colchicine was used as a strategy to overcome the above constraints because it was thought to increase the production of polyploid banana plantlets. Changes in the anatomical and morphological structures are indicators of polyploidy. The purpose of this study was to obtain information on changes in the anatomical and morphological structures of plantain leaves as a result of giving in vitro plantlet propagation media as an indicator of the formation of polyploidy. Plantlets were grown on tissue culture media supplemented with three treatments: 10% flame lily tuber extract, 0.1% pure colchicine, and without addition (control). The parameters observed were epidermal cell size, stomata size, stomata index, number and leaf area. Epidermal cell size, stomata size and stomatal index were analyzed using range values ​​and mean values. The number of leaves, and the average wide of leaf, is then shown in the form of a bar chart. The results showed that administration of flame lily tuber extract was able to increase epidermal cell size, stomata size, wide of a leaf, and able to reduce the stomata index and number of leaves.
RESISTANCE OF RED CURLY CHILI (Capsicum annuum L.) SPROUTS TO FUSARIUM OXYSPORUM INFECTION FROM SEEDS INDUCED BY 0.2 mT Dumayanti, Essy; Agustrina, Rochmah; Setiawan, Wawan Abdullah; Ernawiati, Eti; Yulianty, Yulianty; Chrisnawati, Lili
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Curly red chili (Capsicum annuum L.) is widely used as industrial raw material because it has a spicy taste. The increasing demand for chili is not balanced with the level of production. Chilies are susceptible to disease, one of which is fusarium wilt. The magnetic field affected the physical and chemical properties of water so that it increased peroxidase enzyme activity and plant metabolism. This research was aim to know the protective effect seeds induced by 0.2 mT Magnetic Fiel had given to red curly chili (Capsicum annuum L.) sprouts to Fusarium oxysporum infection. This study was arranged in a completely randomized design (CRD) with 5 treatments consisting of M0F0 (control), M7F0, M7F60, M15F0, M15F60. M0 is a seed not induced by a magnetic field; M7 is seed induced by magnetic field 7 minutes 48 seconds, M15 is seed induced by magnetic field 15 minutes 36 seconds, F0 is sprouted not infected with F. oxysporum and F60 is sprouts infected with F. oxysporum for 60 minutes. Each unit is repeated 5 times. The results of the ANOVA showed that exposure to a magnetic field had a significant effect on increasing plant height at 21, 28, and 35 days after planting (HST); the wet and dry weight of 7 days old plants; and the content of chlorophyll a, b, and total before flowering 21 days after planting. Overall, the 0.2 mT magnetic field treatment for 7 minutes 48 seconds tended to give better results to increase the growth of chili plants against F. oxysporum infection.
The Role of Kolkisin in Multiplication of Planlet Banana Kepok Abu Poliploidi in Vitro Sartika, Dewi; Agustrina, Rochmah; Ernawiati, Eti; Irawan, Bambang
Jurnal Natur Indonesia Vol 18, No 2 (2020): oktober
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.18.2.76-81

Abstract

Bananas are one of the horticultural crops of the fruit group that have quite high social and economic value. One type of banana that is very popular is the pisang kepok. The main obstacle in pisang kepok  production is the availability of seedlings. The above constraints can be overcome by propagation of banana seedlings using tissue culture techniques by adding colchicine to the tissue culture media. Colchicine is often used to improve plant quality. This study aims to determine the effect of adding colchicine to the media on the growth and formation of pisang kepok abu plantlet. The study was conducted from November 2019 - February 2020 at the MTC Laboratory, PT Great Giant Pineapple PG 4, East Lampung. The concentration of colchicine added to the media was 0.1%. The growth and development of plantlets produced from media with colchicine treatment will be compared with plantlets from the control group. The tests were repeated 10 times. The results showed that the growth and formation of pisang kepok abu plantlets from the treatment of colchicine were lower than the growth and formation of the pisang kepok plantlets in the control group.
THE EFFECT OF MAGNETIC FIELD EXPOSURE ON MEDIUM TO PROTEASE PRODUCTION BY Bacillus sp. Sumardi, Sumardi; Agustrina, Rochmah; Irawan, Bambang; Pratiwi, Ajeng
BIOVALENTIA: Biological Research Journal Vol. 4 No. 2 (2018)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.187 KB) | DOI: 10.24233/BIOV.4.2.2018.105

Abstract

This research was purposed to understand the effect of 0.2mT magnetic field exposure treatment for 10 minutes toward medium components to the production of protease in Bacillus sp. That magnetic field exposure treatment was given to 8 medium components namely Milk, Yeast, NaCl, KH2PO4, MgSO4, (NH4)2SO4,Agar and Aquadest. Data from Qualitative Proteolytic Activity test on Bacillus sp. indicated that in all treatment, the bacteria were able to produce the enzyme. The highest Proteolytic Index (IP) from all those treatments came from the magnetically exposed KH2PO4 which was 7.17 at the 10th incubation hour. Treatment of exposure to magnetic fields is also given to the liquid medium. Quantitative data of enzyme activity showed that the best incubation time of protease production by Bacillus sp. is the 24th incubation hours with result of 0.031 U/ml. Exposure of 0.2 mT magnetic field for 10 minutes to the NaCl component in Mendel's fluid medium yielded the highest protease activity of 0.067 U/ml.