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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia MEDIA KONSERVASI Buletin Peternakan ILMU KELAUTAN: Indonesian Journal of Marine Sciences BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Ilmu Ternak dan Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Ilmu dan Teknologi Peternakan Tropis BioCONCETTA Aceh Journal of Animal Science JURNAL BIOLOGI INDONESIA ZOO INDONESIA Jurnal Biologi Tropis ANNALES BOGORIENSES Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Bioma AL-HAYAT: Journal of Biology and Applied Biology Zoo Indonesia

ACHMAD FARAJALLAH

Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor, Indonesia

Author-ID : 222282

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Verification for the control of kappa-casein gene on milk protein yield of friesian-holstein in dairy central region of West Java Anggraeni, A.; Sumantri, C.; Farajallah, A.; Andreas, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Genotipe of BB kappa kasein (Ä¸-kasein) gene positively influences high milk protein yield. The aim of this research was to verify the use of the Ðº-casein gene as marker of assisted selecion (MAS) of high protein yield in domestic Holstein-Friesian (HF) cattle. Genotyping blood samples was conducted for lactating cows from BPPT-SP Cikole (82 head) and a number of small holders in KPSBU Lembang (190 head). Genotyping the Ðº-casein gene was also conducted for active and non active AI bulls (HF) from BIB Lembang (25 head) and BBIB Singosari (32 head). Varian genotipes were identified trough DNA migration, whereas frequencies of alleles were calculated by Nei Method (1987). The effects of variant genotypes on protein yield and other milk component yields were studied in lactating cows (56 head in BPPT-SP Cikole and 111 head in KPSBU Lembang) by applying Least Square Means (LSM) method of the General Linear Model (GLM). Genotyping the Ä¸-kasein gene resulted in three genotypes, namely AA, AB and BB, with two alleles, namely A and B. It was identified that a very limited number of lactating cows with BB genotype (0-9%). These cows produced higher protein yield around 3.37-3.84% than that of AA cows, while AB cows produced protein yield in between, though these differences were not statistically significant (P>0,05). In contrast, AA cows tended to produced higher milk fat yield than BB cows. Cows BB and AB seemingly produced higher dry matter, while both specific density and pH were not affected by variant genotypes of the Ðº-casein gene.Â The results showed that BB genotype of the Ðº-casein gene tended to be quitely consistent in controlling high protein yield, so it would be a good oppurtinity to be used as MAS of milk protein yield in HF domestic. Key words: Protein Yield, Dairy Cattle (HF), Ðº-Casein Gene, Genotype BB

Variation on DNA microsatellite of Holstein-Friesian dairy cows in Baturaden Dairy Cattle Breeding Center Sumantri, C; Anggraeni, A; Farajallah, A; Perwitasari, D
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Eight microsatellite loci of which the locations were three on chromosome 6 (BTA6) namely CSN 3, BM 143 and BM 415; two on chromosome 9 (BTA9) namely ETH 225 and BM 4208; and three on chromosome 10 (BTA10) namely BP 31, BM 1237 and BM 888 were used to investigate genotypic variation of Holstein-Friesian (HF) dairy cows in Baturraden Dairy Cattle Breeding Centre. Research activities were carried out through some steps involving blood collection, DNA isolation, amplification on DNA fragments by polymerase chain reaction (PCR) and separation by electrophoresis with silver staining. Frequency and heterozygosity of genes under consideration were calculated. The results showed that the eight microsatellite loci exhibited a total number of 33 alleles. Locations of those alleles were five in BM 143 and BM 4208 loci; four in BM 415, CSN 3, ETH 225, BM 1237, BM 888 loci; and three in BP 31 locus. A number of 14 out of 33 alleles might be specific alleles for HF dairy cattle in Baturraden Dairy Cattle Breeding Centre. The lowest heterozygosity per locus (Ä¥) was 0.6151 for BM 415 whilst the highest one was 0.7301 for BM 888. Additionally, the average heterozygosity for all loci (Ä¤) detected in this study was 0.6768. The genotype BB on locus BM 143 and AC on locus CSN-3 significantly affected (P<0.05) the estimated breeding value of milk yield of HF cows in this location. Key Words: Holstein-Friesian Cows, DNA Microsatellite, Allele, EBV of Milk Yield

Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Batubara, Aron; Noor, R.R.; Farajallah, A.; Tiesnamurti, B.; Doloksaribu, M.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Indonesian local goats were spread in some region, but there was still limited dataâs known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 Â± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats

Aplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Province Sumantri, Cece; Diyono, R; Farajallah, A; Anggraeni, A; Andreas, E
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

Selection using genetic markers are commonly performed to improve livestock productivity in the livestock industry. The objectives of this study were to identify growth hormone genes family (GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI) polymorphisms of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. A total number of 209 blood samples were collected from 15 districts. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products of GH, GHR, GHRH and Pit-1 Genes were digested with MspI, AluI, HaeIII and HinfI enzyme restriction. Fragments of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI were detected by EtBr method. The results showed that GH|MspI and GHRH|HaeIII loci were polymorphic, GH|AluI, GHR|AluI and Pit-1|HinfI, loci were monomorphic. GH allele (-) at locus GH|MspI was only found in Cisata (0.03) and Menes (0.11). Allele B at locus GHRH|HaeIII only found in Cibadak (0.42), Cisata (0.30) and Menes (0.11). In the total population of Banten locus GH|MspI have low diversity (He = 0.02) and polymorphic information content (Pic = 0.02), whereas GHRH|HaeIII locus has a higher diversity (He = 0.23) and Pic (0.22). Key Words: Polymorphism, Growth Hormone Genes, Buffalo

Polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) Gene at Locus (Pit-1-Hinf1) and its effects on dam body weight and milk production of local sheeps Sumantri, C.; Herdiana, D.; Farajallah, A.; Rahmat, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Pituitary-Specific Transcription Factor-1 (Pit-1) is a transcription factor with critical role in the transcriptional regulation of multiple genes in the pituitary. The objective of this research was to identify polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) gene at Locus (Pit-1-Hinf1) and to investigate any possible associations of Pit-1 genotypes on dam body weight, milk production and milk quality in local sheep at the Jonggol Animal Science Teaching and Research Unit (JASTRU), Fact. Anim. Sci. Bogor. Agric. Univ. A total number of 161 blood samples were collected from 3 local sheep, namely Garut from Wanaraja (55 hd), Garut from Margawati (23 hd) and lactating ewes (83 hd) from JASTRUÂ farm in Bogor. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products were digested with a Hinf1 enzyme restriction. Fragments of Pit-1gene at locus Pit-1-Hinf1 was detected by a silver-staining method. A length of 637 base pairs (bps) of the Pit-1 gene of local sheep was successfully amplified. The Hinf1 restriction enzyme cut the PCR product into three different length of fragments succesively at 345, 137, and 115Â Â bps designated as A allele; whilst B allele had four fragments at 283, 137, 115, and 62 bps respectively. The locus of Pit-1-Hinf1 was polymorphic in local sheep from Jonggol, however it was monomorfic in Garut sheep. The frequencies of A and B alleles were 0,806 and 0,194 respectively. Pit-1 genotypes had no significant effect on dam body weight and milk production. This result is indicating that the use of single locus Pit-1-Hinf1 in Pit-1 gen is less effective to be used as a candidate in selecting dam body weight and milk production in these three local sheep. Key words: Local Sheep, Pit-1 Gene, Polymorphism, Dam, Milk

Polymorphism of Calpastatin gene and its effect on body weight of local sheeps Sumantri, C; Diyono, R; Farajallah, A; Inounu, I
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

The objectives of this research were to identify polymorphism of calpastatin gene and to investigate any association of calpastatin genotype on body weight of local sheeps. A total number of DNA samples were collected from 288 heads of local sheeps from 8 populations. Two local sheep samples were medium tail sheeps (MTSs) of a Garut fighting type from Ciomas/Bogor (29) and a Garut meat type from Margawati (29). The remaining six local sheep population were one thin tail sheep (TTS) from Jonggol (36); and five fat tail (FTSs) from Indramayu (43), Madura (43), Sumbawa (26), Rote (36) and Donggala (46) respectively. Genomic DNAs of those blood of local sheeps were extracted by a standard phenol-chloroform protocol and amplified using a polymerase chain reaction (PCR) technique. PCR reaction was carried out in a thermocycler (Takara PCR of Thermal Cycler MP4) and PCR products were digested with Msp 1 enzyme restriction using a Restriction Fragment Length Polymorphism (RFLP) technique. The PCR-RFLP products were separated at 8% polyacrylamide gel electrophoresis (PAGE).Â A silver-staining method then was applied to detect fragments. Genetic variations between local sheep populations were calculated based on frequencies of genotypes and alelles. The association between genotype of calpastatin gene and body weight of local sheeps were calculated by General Linear Model method by SAS version 6.12. A length of 622 base pairs (bp) of the calpastatin gene of the Indonesian local sheeps was successfully amplified by the PCR technique. An MspI restriction enzyme cut the PCR product into two different length fragments, those were 336 bp and 286 bp designated as M allele of the CAST-Msp1; whilst that unsuccessfully cut PCR product resulted one fragment 622 bp designated as N allele of the CAST-Msp1. Locus of the CAST-Msp1 gene in most local sheeps studied was polymorphic, the exception was in the FTS from Rote of which monomorphic. The highest frequency of the M allele was in the fighting Garut sheep from Ciomas (0.29), whilst the lowest was in the FTS from Rote (0.00). However, frequencies of the M allele of FTSs from Sumbawa and Madura were similar (0.04). Further, frequencies of the M allele of local sheeps from Margawati, Jonggol, Indramayu, and Donggala were 0.24, 0.16, 0.13 and 0.12 respectively. The highest frequency of MN genotype was observed in the Garut fighting sheep from Ciomas (0.58), but the lowest was in the FTS from Rote (0.00). The heterosigosity was observed differentlyÂ among populations. The highest heterosigosity was also identified in the Garut fighting sheep in Ciomas (0.43), whilst FTSs both in Sumbawa and Madura were for the lowest (0.08). Results of this study showed that there was a definit association betwen calpastatin genotype and body weight of male sheeps from which the MN genotype significantly related to a higher body weight compared to that of the NN genotype. Key Words : Local Sheeps, Calpastatin Gene, Polymorphism, Live Weight

EFEKTIVITAS JENIS ALAT TANGKAP DAN JENIS UMPAN YANG DIGUNAKAN UNTUK MENANGKAP UDANG AIR TAWAR DI DANAU KAMPUS INSTITUT PERTANIAN BOGOR Agustina, Meutiya; Farajallah, Achmad; Wowor, Daisy
ZOO INDONESIA Vol 24, No 2 (2015): Desember 2015
Publisher : Masyarakat Zoologi Indonesia

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Studi mengenai penangkapan udang air tawar dengan metode eksperimental fishing telah dilakukan di Danau Kampus Institut Pertanian Bogor. Penelitian ini bertujuan untuk mengetahui efektivitas penangkapan udang air tawar dengan menggunakan alat tangkap Tray net dan bubu yang diberi tiga jenis umpan yang berbeda, yaitu terasi, pelet pakan ikan dan kelapa bakar. Alat tangkap tray net lebih efektif dibandingkan bubu dalam penangkapan udang, namun umpan terasi yang dimasukkan kedalam perangkap bubu adalah umpan yang paling efektif diantara ketiga jenis umpan yang diberikan. Udang air tawar yang diperoleh diidentifikasi dan didapatkan dua jenis udang yaitu Macrobrachium lanchesteri dan Caridina propinqua sebanyak 474 individu.

APLIKASI DNA BARCODE PADA PENENTUAN SPESIES UDANG AIR TAWAR YANG BERASAL DARI PROVINSI JAMBI purnamasari, Lora; Farajallah, Achmad; Wowor, Daisy
Jurnal Bioconcetta Vol 2, No 1 (2016)
Publisher : STKIP PGRI Sumatera Barat

Provinsi Jambi memiliki hutan hujan tropis dataran rendah yang mengalami deforestasi tercepat di daerah Asia. Efek dari perubahan alih fungsi hutan ke habitat pertanian akan mengubah kondisi ekosistem. Hal tersebut diduga berpengaruh pada keanekaragaman makroinvertebrata di dalamnya. Kesalahan identifikasi morfologi dapat disebabkan oleh fenomena cryptic species maupun siblings species. Fenomena tersebut dapat menyebabkan masalah sinonim yaitu terdapat nama ganda pada satu spesies yang sama. Untuk menyelesaikan permasalahan tersebut dapat digunakan teknik DNA Barkode. Penelitian ini bertujuan untuk mengidentifikasi dan memastikan spesies udang air tawar yang berasal dari provinsi Jambi dengan mengaplikasikan teknik DNA barcoding.Berdasarkan identifikasi morfologi didapatkan 3 sampel terdiri dari satu famili. Berdasarkan analisis COI gene, 2 spesies berhasil diidentifikasi yaitu Macrobrachium malayanum dan M. pilimanus. Satu spesies sampel target yaitu M. malayanum 1 terbukti sebagai spesies M. malayanum yang sesuai dengan data GenBank.

Laju Kehilangan dan Kondisi Terkini Habitat Baning Sulawesi (Indotestudo forstenii) di Semenanjung Santigi, Sulawesi Tengah, Indonesia Riyanto, Awal; Soemarno, Suprayogo; Farajallah, Achmad
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTThe Loss and Recent Condition Habitat of Sulawesian Tortoise (Indotestudo forstenii) atCape Santigi, Central Sulawesi, Indonesia. Unsupervised method of remote sensing wasapplied were evaluated to compute the habitat loss during five years, from 2001 to 2005 usingMultispecW32 software. Meanwhile, the fieldwork was done from 22 to 30 June 2007 todetermine the recent condition of habitat at Sologi hill, a part area of Cape Santigi. During fiveyears (from 2001 to 2005) the forest (habitat) in Cape Santigi was loss making up 60.04 % (rate= 419.25 ha/year). Sologi hill forest was shown as a remaining habitat in Cape Santigi. Vegetationin Sologi hill forest is relatively still good, but is threatened by human activities. We suggesteddeveloping the natural preserve to protect this tortoise, habitat and ecosystem also otherwildlife at Sologi hill.Key words: Indotestudo forstenii, habitat, Cape Santigi, Central Sulawesi, Indonesia.

Association between Polymorphism of Î²-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Sumantri, Cece; D, Nurhayati; Farajallah, Ahmad; Anggraeni, A
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The aims of this study were to identify the effects polymorphism of Î²-lactoglobulin geneÂ on milk yield and percentage of milk proteinÂ andÂ fatÂ inÂ local sheep. A total number of 83Â heads ofÂ lactating ewesÂ raised under an extensive management atÂ Jonggol Animal Science Teaching and Research Unit (JASTRU) ofÂ the Faculty of Anima Science, Bogor AgriculturalÂ University wereÂ studied.Â ResearchÂ activitiesÂ wereÂ carriedÂ outÂ throughÂ someÂ step involving blood collection. DNA isolation, DNA amplification and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (PÂ R) then genotyped by single strandÂ conformation polymorphism technique (SSCP), it was success fully amplified a fragment length at 420 bp in Î²-lactoglobulin gene located onÂ the exon 7. The electrophoresis Â patternÂ revealed 5 types of Î²-lactoglobulin gene and designated as AB. C, D and E types. The proportion forÂ those respective 5 type from theÂ highest toÂ the lowest were for A (27.7%)C (16.87%), D (12.05%), (10.84%) and B (9.64%)Â respectively. There were noÂ significant effectÂ polymorphism Î²-lactoglobulin gene on both milk yield and qualityÂ inÂ IÂ calÂ sheep at JASTRU. Â Keywords : polymorphism. Î²-lactoglobulin in gene, PCR-SSCP and local sheep

Co-Authors A Anggraeni A Anggraeni A Anggraini A Batubara A. Anggraeni A. Anggraeni Agus Alim Hakim Akhsani, Farid AKICHIKA MIKAMI Ali Mashar Ali Suman Amir Hamidy Amir Hamidy AMIR HAMIDY ANIFA BINTAR Annawaty Annawaty, Annawaty Annissa Nuridfi Sasmita Arindina Mahendra Arma Aditya Kartika Aron Batubara Aron Batubara Asri Febriana B Tiesnamurti B. Tiesnamurti Baiq Farhatul Wahidah Bambang Purwantara Bambang Suryobroto Burhanuddin Burhanuddin C Sumantri C Sumantri C. Sumantri C. SUMANTRI Cece Sumantri D Perwitasari D, Nurhayati D. Herdiana D. Rahmat Daisy Wowor Daisy Wowor Daisy Wowor, Daisy Dede Setiadi Devi Sandriliana DEWI APRI ASTUTI DEWI PURNAMA Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH E Andreas E. Andreas E. Andreas Eric N. Smith Eric N. Smith Erni Sulistiawati Eryk Andreas H. Nuraini HERA MAHESHWARI I Inounu Imas Batubara Irma Shita Arlyza Isdoni Bustaman J F Salamena Jarmuji Jarmuji Jeane Siswitasari Mulyana Joni Setiawan KANTHI ARUM WIDAYATI Khairiza Lubis Koekoeh Santoso Koekoeh Santoso KREY KREY La Jumadin Lora purnamasari, Lora Luthfia Nuraini Rahman M Doloksaribu M Karmita M. CHANDRA WIDJAJA M. Doloksaribu Mennofatria Boer Meutiya Agustina Meutiya Agustina, Meutiya Mufti Sudibyo N Satriani Neviaty P Zamani Nirmala Fitria Firdhausi Nisfa Hanim Nisfa Hanim Novita Anggraeni Nurhayati D Nurlisa Butet, Nurlisa Nurulvadini Safrudin Person Pesona Renta Pradana, Tengku Gilang Pudji Achmadi R Diyono R Misrianti R R Noor R R Noor R.R. Noor R.R. Noor Rarah Ratih Adjie Maheswari RIKA RAFFIUDIN Rissa Herawati Ginting Riyanto, Awal Riyanto, Awal Rose Ida Ummah Rudi Alek Wahyudin Silvia Puspitasari Siti Lutvaniyah Slamet Mulyanto Smith, Eric N. Soemarno, Suprayogo Soemarno, Suprayogo TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TARUNI SRI PRAWASTI TATI NURHAYATI Vella Nurazizah Djalil VELLA NURAZIZAH DJALIL Wahyudin Abd. Karim Yanti Ariyanti Yanti Ariyanti Yusli Wardiatno

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