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Hubungan Filogenetik Phrynella pulchra Boulenger, 1887 Berdasarkan Gen 16S rRNA Akhsani, Farid; Hamidy, Amir; Farajallah, Achmad; Smith, Eric N.
ZOO INDONESIA Vol 26, No 2 (2017): Desember 2017
Publisher : Masyarakat Zoologi Indonesia

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Abstract

Phrynella merupakan marga monotipik yang sejauh ini hanya terdiri dari satu jenis, yakni Phrynella pulchra. Jenis ini terdistribusi di Semenanjung Malaysia, Sumatra dan Pulau Mentawai. Kami mengevaluasi status taksonomi populasi yang berasal dari Sumatra menggunakan data sekuen dari gen 16S rRNA mitokondria. Hubungan filogenetik dianalisis menggunakan Neighbour Joining (NJ), Maximum Likelihood (ML), Bayesian Inference Analisys (BI) dan Unweighted Pair Group Method with Arithmetic Means (UPGMA). Hasil penelitian menunjukkan bahwa P. pulchra adalah kelompok monofiletik terhadap anggota luar (Metaphrynella, Kaloula dan Micryletta). Kelompok monofiletik P. pulchra terbagi menjadi dua kelompok besar: Sumatra dan Semenanjung Malaysia. Kelompok Sumatra terdiri dari dua subkelompok: Aceh dan Sumatra Utara- Bengkulu. Jarak genetik (uncorrected p-distance) antara populasi Semenanjung Malaysia dan Sumatra berkisar antara 1,1 sampai 2,0%, sedangkan di dalam populasi Sumatra berkisar antara 0,0 sampai 1,1%. Rendahnya jarak genetik populasi Semenanjung Malaysia dengan Sumatra secara taksonomi menunjukkan populasi tersebut masih berada pada tingkat Jenis yang sama.
Identifikasi Molekuler Microhyla, Tschudi 1839 dari Sumatera Berdasarkan Gen 16S rRNA Pradana, Tengku Gilang; Hamidy, Amir; Farajallah, Achmad; Smith, Eric N.
ZOO INDONESIA Vol 26, No 2 (2017): Desember 2017
Publisher : Masyarakat Zoologi Indonesia

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Abstract

Penelitian ini bertujuan mengevaluasi posisi jenis Microhyla dari Sumatra berdasarkan sekuen data dari gen 16S rRNA di dalam pohon filogeni Microhyla secara umum. Pohon filogeni dihasilkan dengan menggunakan analisis Unweighted Pair Group Method with Aritmatic Means (UPGMA), Neighbor Joining (NJ), Maximum Likelihood (ML) dan Bayesian Inference (BI). Kami menggunakan Phrynella pulchra, Kaloula pulchra, Kalophrynus pleurostigma, Chalcorana chal-conota, dan Leptobrachium waysepuntiense sebagai anggota outgroup. Hasil pohon filogeni menunjukkan bahwa Micro-hyla terdiri dari sembilan kelompok utama, enam diantaranya merupakan Microhyla dari Sumatra. Microhyla dari Su-matra mengelompok dengan M. achatina, kelompok M. heymonsi, kelompok M. fissipes, kelompok M. palmipes, kelompok M. berdmorei dan kelompok M. superciliaris. Beberapa kelompok memiliki jarak genetik yang besar seperti pada M. achatina (3,1−3,4%), M. heymonsi (3,3−7,1%) dan M. palmipes (2,5−3,4%). Microhyla fissipes membentuk parafiletik dengan M. mukhlesuri, kemungkinan termasuk kedalam complex atau cryptic spesies. Hal ini membutuhkan kajian lebih lanjut secara morfologi dan suara guna menentukan status taksonomi dari masing masing populasi di dalam jenis-jenis tersebut.
LAJU KEHILANGAN DAN KONDISI TERKINI HABITAT BANING SULAWESI (INDOTESTUDO FORSTENII) DI SEMENANJUNG SANTIGI, SULAWESI TENGAH, INDONESIA Riyanto, Awal; Soemarno, Suprayogo; Farajallah, Achmad
JURNAL BIOLOGI INDONESIA Vol 6, No 2 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i2.3158

Abstract

ABSTRACTThe Loss and Recent Condition Habitat of Sulawesian Tortoise (Indotestudo forstenii) atCape Santigi, Central Sulawesi, Indonesia. Unsupervised method of remote sensing wasapplied were evaluated to compute the habitat loss during five years, from 2001 to 2005 usingMultispecW32 software. Meanwhile, the fieldwork was done from 22 to 30 June 2007 todetermine the recent condition of habitat at Sologi hill, a part area of Cape Santigi. During fiveyears (from 2001 to 2005) the forest (habitat) in Cape Santigi was loss making up 60.04 % (rate= 419.25 ha/year). Sologi hill forest was shown as a remaining habitat in Cape Santigi. Vegetationin Sologi hill forest is relatively still good, but is threatened by human activities. We suggesteddeveloping the natural preserve to protect this tortoise, habitat and ecosystem also otherwildlife at Sologi hill.Key words: Indotestudo forstenii, habitat, Cape Santigi, Central Sulawesi, Indonesia.
IDENTIFICATION OF GH|ALUI AND GHR|ALUI GENES POLYMORPHISMS IN INDONESIAN BUFFALO Andreas, E.; Sumantri, C.; Nuraini, H.; Farajallah, A.; Anggraeni, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 35, No 4 (2010): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.35.4.215-221

Abstract

Growth hormone (GH) is an anabolic hormone which sintesized and secreted by somatrotop cell inpituitary anterior lobe. GH exert its effect on growth and metabolism by interacting with a specificreceptor on the surface of the target cells. Growth hormone receptor (GHR) has been suggested ascandidate gene for traits related to meat production in Bovidae. The objectives of this study were toidentify polymorphism of GH and GHR genes in buffalo. The 452 DNA samples buffalo were collectedfrom five populations in Indonesia (Siborong-Borong-Medan (65), Lebak-Banten (29), Pandeglang-Banten (180), Semarang-Central Java, and Mataram-West Nusa Tenggara (103)). A gene fragment of theGH|AluI gene at 432 bp located on exon 3 and GHR|AluI gene at 298 bp on exon 10 were successfullyamplified by using the techniques of a PCR (polymerase chain reaction) and genotyped by PCR-RFLP(restriction fragment length polymorphism) then -SSCP (single strand conformation polymorphism). Theresults showed no polymorphisms were detected in these genes. All buffaloes tested had LL genotype forlocus GH|AluI and AA genotype for locus GHR|AluI.
Human brain posseses the ability to create a concept to assist the process of grouping individual object or events into different classes or categories. We call this grouping process as categorization. In addition to humans, the ability to categorize has also been proposed for animals. Being able to identify, visually or otherwise, a new object as a member of a category is an advantage for animals. Present experiment aims to test the categorization ability in discriminating species by Macaca fas KANTHI ARUM WIDAYATI; BAMBANG SURYOBROTO; ACHMAD FARAJALLAH; AKICHIKA MIKAMI
HAYATI Journal of Biosciences Vol. 18 No. 4 (2011): December 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.177

Abstract

Human brain posseses the ability to create a concept to assist the process of grouping individual object or events into different classes or categories. We call this grouping process as categorization. In addition to humans, the ability to categorize has also been proposed for animals. Being able to identify, visually or otherwise, a new object as a member of a category is an advantage for animals. Present experiment aims to test the categorization ability in discriminating species by Macaca fascicularis. Using match-to-sample task with photographs of monkeys and human as stimuli, we tested whether monkeys able to categorize monkey individuals as a class against human individuals as another class. We found that monkeys categorized humans differently from monkeys. The monkeys used physical characteristic such as shape and colors from the photographs to create different concepts of human and monkeys.
Skin Histology and Microtopography of Papuan White Snake (Micropechis ikaheka) in Relation to Their Zoogeographical Distribution KREY KREY; ACHMAD FARAJALLAH
HAYATI Journal of Biosciences Vol. 20 No. 1 (2013): March 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.37 KB) | DOI: 10.4308/hjb.20.1.7

Abstract

Papuan white snake (Micropechis ikaheka) is endemic to New Guinea (the region of the Papua and Papua New Guinea). Internal histology of skin layer and the microtopography structure on the surface scales of M. ikaheka were the aims of the study. This study also related to zoogeographical of the snake in Papua. Geographical skin color variation of M. ikaheka can be described in three groups, i.e. brown, yellow and black group. The three groups of the snake have specific zoogeography in the mainland of Papua and satellite islands to Papua New Guinea. Paraffin method used in this work showed approximately five layers i.e. oberhautchen, the beta (b)-layer, the mesos layer, the alpha (a)-layer, and the dermis in the snake skin. Although the paraffin method cannot explain the arrangement of pigment cells, however, the dark color on the dermis might contain melanophores that might cause dark color of the snake body. Overlapping scales formed the hinge region were flexible to assist the snakes when they moved across substrate. Scanning electron microscopy (SEM) of the oberhautchen of all of M. ikaheka revealed no microornamentation. However, dorsal and ventral scales showed many follicles on the entire surface of the boundary scales.
Three Species of Ectoparasite Mites (Acari: Pterygosomatidae) Infested Geckos in Indonesia TARUNI SRI PRAWASTI; ACHMAD FARAJALLAH; RIKA RAFFIUDIN
HAYATI Journal of Biosciences Vol. 20 No. 2 (2013): June 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.433 KB) | DOI: 10.4308/hjb.20.2.80

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Limited data is hitherto available on the diversity and dispersal of parasitic mites of geckos in Indonesia. Here, we collected three species of geckos, namely Cosymbotus platyurus, Hemidactylus frenatus, and H. garnotii throughout Indonesia to study the distribution and diversity of its parasitic mites. We conducted detail morphological analysis of the mites using whole mount polyvinyl lactophenol and scanning electron microscope preparation. Three species of ectoparasite mites from genus Geckobia were identified in a total of 221 individuals out of 448 geckos collected from 25 sites in Indonesia. Two species were G. glebosum and G. bataviensis, and the other one was designated as Geckobia sp 1. Based on our result, the three mites species were spread randomly and live sympatrically. The G. bataviensis mite showed the widest distribution, because it was found in almost all gecko collection sites, hence the most cosmopolitan mites. We also found that C. platyurus gecko had the lowest mite prevalence which might due to the fact that it has the least number of skin folds, an important site for mite protection. This result implies that further research on the relationship of anatomy of gecko skin with chelicera and claw structure of mites is necessary in the future.
Microsatellite DNA Analysis on the Polyandry of Green Sea Turtle Cheloniamydas DEWI PURNAMA; NEVIATY PUTRI ZAMANI; ACHMAD FARAJALLAH
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (695.098 KB) | DOI: 10.4308/hjb.20.4.182-186

Abstract

Green turtle (Cheloniamydas; Testudines) is included in the group of polyandryanimals, which is single female mated with many male. DNA polymorphism method generally considered to have a high degree of accuracy as compared to other methods to elucidate polyandry phenomena on many animals. In this research, three microsatellite loci were used to identify the number and frequency of genotypes per locus, the number and frequency alleles per locus, and genotypes and number of alleles in the nest. The purpose of this research was to study the reproductive pattern of Cheloniamydas and compensation eggs of males from hatchling’s population in turtle conservation area of Pangumbahan Coastal Park, West Java. The result showed that from 10 nests, we could find 37 genotypes with 11 alleles for D108 locus, 21 genotypes with 9 alleles for B103 locus, and 27 genotypes with 9 alleles for C102 locus. The alleles number of each nest was more than 5 alleles for 5 nests, and more than 4 alleles for the remaining nests. Based on the probabilities of alleles contribution of each parent, the green turtle was polyandry animals.
Habitat Preferences and Distribution of the Freshwater Shrimps of the Genus Caridina (Crustacea: Decapoda: Atyidae) in Lake Lindu, Sulawesi, Indonesia Annawaty Annawaty; Daisy Wowor; Achmad Farajallah; Dede Setiadi; Bambang Suryobroto
HAYATI Journal of Biosciences Vol. 23 No. 2 (2016): April 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1233.575 KB) | DOI: 10.4308/hjb.23.2.45

Abstract

The objectives of this study were to reveal ecological preferences and distribution of all species of Caridina found in Lake Lindu and their catchment area. Specimens from 39 sampling sites were caught using tray net and hand net. There are three species of Caridina found in the lake system, i.e. Caridina linduensis, Caridina kaili and Caridina dali. There is no overlapping distribution among the species. Caridina kaili is a true riverine species and it is never encountered sympatric with C. dali nor C. linduensis. This species is abundant in streams and ditches with moderate flow running water and gravel–cobble substrate. It is mainly spread within streams west to the lake. Both C. daliand C. linduensis can be found in the lake and streams with very slow current to almost stagnant water, muddy sand substrate and associated with roots of water plants and leaf litter. However, C. dali is never occurred together with C. linduensis and they are less abundant compare to C. kaili. Distribution of Caridina spp. in Lake Lindu is probably affected by the temperature of their habitats and the occurrence of introduced fish such as Mozambique tilapia (Oreochromis mossambicus), common carp (Cyprinus carpio), and an alien riceland prawn (Macrobrachium lanchesteri). These introduced and alien species can have the potency to become predators or competitors for the Caridina spp. It is also the first record for M. lanchesteri present in Lake Lindu.
Keragaman Genetik DNA Mikrosatelit dan Hubungannya dengan Performa Bobot Badan pada Domba Lokal C Sumantri; A Farajallah; J F Salamena
Media Peternakan Vol. 31 No. 1 (2008): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.303 KB)

Abstract

The objectives of this study were to evaluate polymorphism of microsatellite DNA through the investigation of microsatellite loci of CSSM18, ILST54 and IDVGA30 and its possible association with body weight of local sheeps. A total of 266 head of DNA samples was collected from 8 local sheep populations, i.e. Garut fighting type from Ciomas-Bogor (25); Garut meat type from Margawati-Garut (29); thin tail sheep (TTS) from Jonggol-Bogor (33); and fat tail sheep (FTSs) from Indramayu (40), Madura (34), Sumbawa (26), Rote (35) and Donggala (44). Genetic polymorphism among groups of local sheep was calculated in frequency of alleles and genotypes. Association between genotypes and body weight was calculated by general linear model (GLM). Results showed that three loci showed high polymorphisms. CSSM18 locus had 12 genotypes with the highest frequency for CC (46,15%) then for AC (25,64%); whilst frequency of the other 10 genotypes providing AA, AE, BB, BC, BD, BE, CD, CE, DD and EE were less than 7%. IDVGA30 locus had 9 genotypes with three highest frequencies for DD (39,10%), CC (37,97%) and BB (10,90%); while the other 6 genotypes were less than 6 %. ILST54 locus had 8 genotypes with three highest frequencies for AA (39,85%), BB (21,43%) and BC (18,05%) respectively. It could be verified that local sheep possessing typical genotypes of CE in CSSM18 locus and CD in IDVGA30 locus was apparently related with a higher body weight compared to another genotypes; on the contrary, those possessing DD genotypes in ILSTS54 was seemingly associated with a lower body weight. Key words: local sheep, microsatellite DNA, genotypes, body weight
Co-Authors A Anggraeni A Anggraeni A Anggraini A Batubara A. Anggraeni A. Anggraeni Agus Alim Hakim Akhsani, Farid AKICHIKA MIKAMI Ali Mashar Ali Suman Amir Hamidy Amir Hamidy AMIR HAMIDY ANIFA BINTAR Annawaty Annawaty, Annawaty Annissa Nuridfi Sasmita Arindina Mahendra Arma Aditya Kartika Aron Batubara Aron Batubara Asri Febriana B Tiesnamurti B. Tiesnamurti Baiq Farhatul Wahidah Bambang Purwantara Bambang Suryobroto Burhanuddin Burhanuddin C Sumantri C Sumantri C. Sumantri C. SUMANTRI Cece Sumantri D Perwitasari D, Nurhayati D. Herdiana D. Rahmat Daisy Wowor Daisy Wowor Daisy Wowor, Daisy Dede Setiadi Devi Sandriliana DEWI APRI ASTUTI DEWI PURNAMA Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH E Andreas E. Andreas E. Andreas Eric N. Smith Eric N. Smith Erni Sulistiawati Eryk Andreas H. Nuraini HERA MAHESHWARI I Inounu Imas Batubara Irma Shita Arlyza Isdoni Bustaman J F Salamena Jarmuji Jarmuji Jeane Siswitasari Mulyana Joni Setiawan KANTHI ARUM WIDAYATI Khairiza Lubis Koekoeh Santoso Koekoeh Santoso KREY KREY La Jumadin Lora purnamasari, Lora Luthfia Nuraini Rahman M Doloksaribu M Karmita M. CHANDRA WIDJAJA M. Doloksaribu Mennofatria Boer Meutiya Agustina Meutiya Agustina, Meutiya Mufti Sudibyo N Satriani Neviaty P Zamani Nirmala Fitria Firdhausi Nisfa Hanim Nisfa Hanim Novita Anggraeni Nurhayati D Nurlisa Butet, Nurlisa Nurulvadini Safrudin Person Pesona Renta Pradana, Tengku Gilang Pudji Achmadi R Diyono R Misrianti R R Noor R R Noor R.R. Noor R.R. Noor Rarah Ratih Adjie Maheswari RIKA RAFFIUDIN Rissa Herawati Ginting Riyanto, Awal Riyanto, Awal Rose Ida Ummah Rudi Alek Wahyudin Silvia Puspitasari Siti Lutvaniyah Slamet Mulyanto Smith, Eric N. Soemarno, Suprayogo Soemarno, Suprayogo TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TARUNI SRI PRAWASTI TATI NURHAYATI Vella Nurazizah Djalil VELLA NURAZIZAH DJALIL Wahyudin Abd. Karim Yanti Ariyanti Yanti Ariyanti Yusli Wardiatno