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All Journal Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Intan Ahmad
Department of Biology Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

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Alteration of Ecdysteroid Titre by Thyroxine and Juvenile Hormone Analogue (Methoprene) in Bombyx mori (Lepidoptera: Bombycidae) Ahmad, Intan; Rahayu, Resti; Permana, Agus D.; Astari, Sita
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.499 KB) | DOI: 10.24002/biota.v12i2.2666

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh hormon tiroksin dan analog hormon juvenil (metopren) terhadap pola titer hormon ekdisteroid hemolimf selama instar lima Bombyx mori. Instar dua B. mori diberi pakan daun murbei yang telah diberi tiroksin dengan konsentrasi 1500 ppm. Larva kemudian dibiarkan ganti kulit sampai instar tiga dan empat tanpa perlakuan. Tujuh puluh dua jam setelah ganti kulit pada instar lima, larva disuntik sebanyak 10 μl metopren dengan konsentrasi 15 atau 25 ppm. Titer ekdisteroid hemolimf dideteksi dengan Kromatografi Cair Kinerja Tinggi. Hasil yang diperoleh menunjukkan bahwa perlakuan dengan tiroksin ataupun kombinasi tiroksin-metopren menyebabkan terjadinya perubahan pola titer ekdisteroid dibandingkan dengan kontrol. Ekdisteroid terdeteksi dua hari lebih awal pada perlakuan tiroksin dan titer ekdisteroid pada puncak tertinggi lebih tinggi jika dibandingkan dengan kontrol. Kombinasi tiroksin-metopren tampaknya menyebabkan penekanan terhadap titer ekdisteroid. Hal ini terlihat dengan terjadinya penurunan titer ekdisteroid segera setelah penyuntikan dengan metopren sebelum mencapai titer puncak tertinggi.
Efisiensi Makan Spodoptera exigua (Lepidoptera: Noctuidae) pada Bawang Daun, Sawi Hijau dan Seledri di Laboratorium Hariani, Nova; Ahmad, Intan; Rahayu, Resti
Jurnal Natur Indonesia Vol 14, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (73.523 KB) | DOI: 10.31258/jnat.14.1.86-89

Abstract

The aim of this research is to gain further understanding of how particular insect species respons to different host plants. This research isconsidered as preliminary research in integrated pest management for Spodoptera exigua. We report herein the consumption and utilizationof green onion (Allium fistulosum), caisin (Brassica rapa subsp. Parachinensis) and celery (Apium graveolens) by Spodoptera exigua. Theevaluation of indices of food consumption, growth and food utilization showed that caisin (Brassica rapa subsp. Parachinensis) was the bestfood for the last instar larvae of S. exigua as compared with celery or green onion. Growth rates and efficiency of conversion of ingestedfood to biomass (ECI) were significantly higher for larvae fed caisin (11.93%) than for those given green onion (8.06%) or celery (7.74%)Although the protein content of caisin (2.23%) was a bit lower than that of green onion (2.45%) but higher than celery (1.28%), we suspectthat the larvae performed best in caisin due to good balance between protein and carbohydrate as well as water content in caisin as comparedwith other host plants.
Co-Authors Nova Hariani Permana, Agus D. Resti Rahayu Sita Astari