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Activity and Stability of the Alcohol Biosensor Using Acetobacter aceti Biofilm on Screen-Printed Carbon Electrode Dyah Iswantini; Fitriani Indahsari; Akhiruddin Maddu; Novik Nurhidayat; Henny Purwaningsih; Sri Sugiarti
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (865.056 KB) | DOI: 10.4308/hjb.27.1.24

Abstract

Most of the alcohol analytical methods are robust and instrumentally expensive. An alternative of ethanol biosensor based on selected biofilm forming Acetobacter aceti bacteria producing alcohol oxidase was constructed on a screen-printed carbon electrode. The enzyme specifically oxidizes the ethanol and generate electrical current that then electrochemically detected and measured by cyclic voltammetry method. A scanning electron microscopic analysis indicated that the biofilm was formed firmly in the electrode. This constructed biosensor reached its optimum at biofilm formed by bacteria of 1.33 × 1010 cells/ml, temperature of 27°C, and pH 7. The enzyme kinetic had KM and Vmax AOX values of 3.5 mm and 125 μA respectively. The biosensor had detection and quantization limit of 0.003 and 0.009%, respectively, and a sensitivity of 57.29 μA (%)-1. A linearity and relative deviation value were revealed at 0.993 and 1.95% respectively. The biosensor was relatively specific and had no interferences with methanol, sodium chloride and citric acid as the common interferences of ethanol compounds. Furthermore, the biosensor had been stably for at least 55 days. Therefore, this constructed biosensor should be developed into a prototype for a practical effective analysis.
Pemanfaatan Alat Deteksi Bunyi untuk Menduga Kadar Air dan Viabilitas Benih Kedelai (Glycine max L. Merrill) Nurul Rostami Dewi; Muhamad Rahmad Suhartanto; Akhiruddin Maddu
Buletin Agrohorti Vol. 1 No. 4 (2013): Oktober 2013
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (453.449 KB) | DOI: 10.29244/agrob.1.4.45-50

Abstract

Deteksi cepat kadar air dan viabilitas benih sangat penting dalam teknologi benih. Pemanfaatan bunyi yang dihasilkan benih bila dipantulkan dengan benda lain adalah salah satu cara yang belum diteliti. Penelitian ini bertujuan mempelajari pemanfaatan alat deteksi bunyi untuk menduga kadar air dan viabilitas benih kedelai (Glycine max L. Merrill) dengan melihat frekuensi gelombang bunyi yang dihasilkan. Penelitian ini terdiri dari dua percobaan, percobaan pertama mempelajari pengaruh antara ukuran benih dan kadar air terhadap frekuensi bunyi yang dihasilkan dari pantulan benih. Percobaan kedua mempelajari pengaruh antara ukuran benih dan viabilitas terhadap frekuensi bunyi yang dihasilkan dari pantulan benih. Penelitian ini menggunakan rancangan acak lengkap dengan dua faktor yaitu ukuran benih + kadar air dan ukuran benih + viabilitas. Hasil menunjukan bahwa pada percobaan pertama terdapat interaksi antara ukuran benih dan kadar air. Ukuran benih sedang memiliki korelasi yang positif antara kadar air dan ukuran benih terhadap frekuensi dan memiliki nilai korelasi (r) 0.96 yang mendekati 1 (≈1), artinya semakin tinggi kadar air maka frekuensi pantulan bunyi yang dihasilkan semakin tinggi. Percobaan kedua tidak terdapat interaksi antara ukuran benih dan viabilitas. Ukuran benih sedang memiliki nilai frekuensi tertinggi yaitu 482.36 Hz.
SINTESIS DAN SIFAT MEKANIK BIOKOMPOSIT SERAT HELICONIA-POLIESTER Akhiruddin Maddu
Jurnal Biofisika Vol. 8 No. 2 (2012): Jurnal Biofisika
Publisher : Jurnal Biofisika

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Abstract

In this study, polyester-fiber heliconia biocomposites has been created and tested on its mechanical properties. Biocomposites was manufactured begins by separating the fibers.  it  consists  of  three  stages,  namely  chlorization,  alkaline  leaching  and extraction.  After  that,  the  manufacture  of  composites  made  with  Hand  Lay-up techniques.  Three  samples  with  variations in  fiber content,  ie without  the addition of fiber,  0.54  g  and  2.08  g  fiber.  Characterization  of  samples  includes  tensile  test, hardness,  and  impact.  The test results known that  the  tensile  strength  and  hardness increased  with  increasing  the  amount  of fiber  heliconia.  Impact  test  shows  sample values optimum working temperature is 10°C
PENGUJIAN SIFAT FISIS-MEKANIS DAN NONDESTRUKTIF METODE GELOMBANG SUARA PAPAN WOL SEMEN BERKERAPATAN SEDANG-TINGGI BAMBU BETUNG (DENDROCALAMUS ASPER) Lina Karlinasari; Dede Hermawan; Akhiruddin Maddu; M. Farouq Iksan; Anita Firmanti
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Cement board is a composite wood product has advantages such as fire resistance, termite, water resistant, and has good sound insulation properties. Bamboo is suitable material that can be used as a raw material of cement board. The objectove of this study was to determine the physical-mechanical properties of wool cement boards from betung bamboo (Dendrocalamus asper) in various densities. Mechanical testing included nondestructive testing performed using the sound waves velocity method (stress wave velocity). The material used was particles of wool/ excelsior dimensions. The adhesive used was Portland cement type I by the ratio of bamboo: cement: water 1:2:1. Target board densities were 0.5 g/cm3; 0.8 g/cm3; and 1.0 g/cm3. The results showed the higher density of the board the higher mechanical properties of boards. The difference density cement board was more influential on mechanical properties (MOE, MOR, SW) board compared with those physical properties. Nondestrcutive testing using sound waves velocity method can be used to predict mechanical bending properties of wool cement board of betung bamboo as indicated by the close relationship between the parameter estimators (dynamic MOE) and static testing value (MOE and MOR). 
Deteksi Cepat Fusarium sp. pada Benih Kedelai Menggunakan Metode Spektroskopi Fluoresens Djoko Pujiarto; Bonny Poernomo Wahyu Soekarno; Akhiruddin Maddu
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.597 KB) | DOI: 10.14692/jfi.14.3.97

Abstract

Rapid Detection Method for Fusarium sp. on Soybean Seed Using Fluorescence Spectroscopy MethodSeed borne pathogens play an important role as source of inoculum for disease in the field.  Seed health testing is applied in order to prevent risks caused by seed borne pathogen. Fluorescence spectroscopy is a potential technology to be used as detection method for seed borne pathogen. Research was conducted to develop rapid detection protocol for seed borne pathogenic fungus by fluorescence spectroscopy method.  The result showed that fluorescence spectroscopy could detect fluorescence emission of metabolite of Fusarium sp. after soybean seeds were incubated for 24 hr.  Metabolite of Fusarium sp. produced cyan fluorescent at peak wavelength emission 504 nm when excited to violet light (405 nm).  Fusarium sp. displayed typical fluorescence emission spectra which differ from fluorescence emission spectra of growth medium potato dextrose broth (PDB) (502 nm). It was evidenced that fluorescence spectroscopy method can be used to detect pathogenic seed borne fungi.
FAKTOR RASIO C/N AWAL DAN LAJU AERASI PADA PROSES CO-COMPOSTING BAGASSE DAN BLOTONG Andes Ismayana, Nastiti Siswi Indrasti, Suprihatin, Akhiruddin Maddu, Aris Fredy TIP
Jurnal Teknologi Industri Pertanian Vol. 22 No. 3 (2012): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

Sugar cane industry generates wastes, such as bagasse and filter cake which were still not used optimally. One of the alternative utilizations is through the co-composting process to produce compost. The objectives of the study were to utilize bagasse and filter cake from the sugar industry waste for composting and to determine the effect of aeration and C/N ratio to the process and the quality of compost. Method of composting was carried out by using the aerated static pile with two factors, i.e. aeration and C/N ratio. Aeration was given in the form of active aeration for 1 hour in the first week, of 0.4 and 1.2 l / min.kg, and C/N ratio of 30, 40, and 50 for both aerations. Co-composting process had formed mesophilic temperature with the highest at 38 oC, with a range of pH 6.0-7.5 at day 22 and then constant in the range of 6.8-8.0. C/N ratio final compost produced was 17-25, with the biggest C/N ratio decreases at the initial C/N ratio of 50, whereas the aeration rate of 1.2 l/min.kg gave bigger changes in C / N ratio.Keywords: co-composting, bagasse, blotong, aeration
PENGARUH UKURAN SERAT SELULOSA ASETAT DAN PENAMBAHAN DIETILEN GLIKOL (DEG) TERHADAP SIFAT FISIK DAN MEKANIK BIOPLASTIK Nur Alim Bahmid, Khaswar Syamsu dan Akhiruddin Maddu TIP
Jurnal Teknologi Industri Pertanian Vol. 24 No. 3 (2014): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

ABSTRAK   Kesadaran lingkungan menjadi pendorong utama dalam pengembangan bioplastik sebagai pengganti plastik konvensional. Salah satu bahan baku potensial bioplastik yaitu selulosa, tetapi memiliki sifat mekanik bioplastik yang masih belum sebanding dengan plastik konvensional sehingga perlu upaya untuk perbaikan. Kekuatan mekanik pada serat selulosa dipengaruhi oleh diameter serat sehingga diperlukan pengecilan ukuran hingga menjadi nanofiber. Tujuan dari penelitian ini  adalah membandingkan sifat mekanik bioplastik dari mikrofiber dan nanofiber selulosa asetat dan mengetahui pengaruh penambahan pemlastis dietilen glikol (DEG) terhadap sifat mekanik bioplastik yang dihasilkan. Penelitian ini didahului oleh pembuatan selulosa asetat dari selulosa tandan kosong kelapa sawit dan sintesis nanofiber selulosa asetat dengan metode electrospinning. Penelitian utama adalah pembuatan bioplastik menggunakan metode solution casting dengan perlakuan ukuran serat selulosa asetat (mikrofiber atau nanofiber) dan penambahan pemlastis DEG (0,10, 20 dan 30%). Hasil penelitian ini menunjukkan bahwa sifat mekanik bioplastik nanofiber lebih baik dibanding microfiber selulosa asetat. Penambahan pemlastis meningkatkan elongasi, daya serap air dan densitas tetapi menurunkan kuat tarik dan modulus elastisitas bioplastik. Berdasarkan sifat fisik dan mekanik, perlakuan terbaik dihasilkan pada bioplastik nanofiber selulosa asetat dengan konsentrasi pemlastis 10%. Sifat mekanik bioplastik yang dihasilkan adalah kuat tarik 18,560 MPa, elongasi 3,953%, modulus elastisitas 0,676 GPa, daya serap air 16,772% dan densitas 1,045 g/cm3. Kata kunci : selulosa asetat, bioplastik, nanofiber, electrospinning, sifat mekanik
PERBANDINGAN KINERJA PROSES CO-COMPOSTING BAGAS DAN BLOTONG PADA SKALA 100 KG TERHADAP SKALA LABORATORIUM Andes Ismayana, Nastiti Siswi Indrastri, Suprihatin, Akhiruddin Maddu, Subiyantoro TIP
Jurnal Teknologi Industri Pertanian Vol. 24 No. 3 (2014): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

ABSTRAK Penelitian untuk menentukan kondisi optimum proses co-composting bagas dan blotong pada skala laboratorium telah dilakukan. Namun, untuk dapat diaplikasikan di industri perlu dilakukan evaluasi terhadap kinerja co-composting dengan membandingkannya pada skala yang lebih besar. Tujuan penelitian ini adalah membandingkan kinerja proses co-composting bagas dan blotong pada skala 100 kg dengan skala laboratorium.  Parameter kinerja utama yang dievaluasi meliputi kinetika proses berupa laju pengomposan serta pengaruh aerasi terhadap laju penurunan C/N. Peningkatan skala co-composting dilakukan dengan memperbesar kapasitas produksi dari 5 kg (skala laboratorium) menjadi 100 kg. Faktor yang dipertahankan berupa kesamaan geometri reaktor, laju aerasi dan nilai C/N awal. Penelitian dilakukan selama 60 hari dengan nilai C/N awal sebesar 50 dan pemberian aerasi aktif sebesar 0,4 L/menit.kg bahan selama 1 jam perhari pada minggu pertama.  Pengamatan proses pada skala 100 kg menunjukkan kondisi proses yang sama dengan skala laboratorium dengan pencapai suhu mesofilik, pH sekitar netral, kadar air optimum, dan peningkatan nitrat dimulai pada minggu kedua.  Berdasarkan hasil penelitian juga didapatkan proses co-composting pada kapasitas 100 kg memberikan laju pengomposan yang lebih rendah dibandingkan dengan kapasitas 5 kg. Selain itu perlakuan aerasi selama proses co-composting kapasitas 100 kg tidak memberikan pengaruh terhadap laju penurunan C/N. Pencapaian mutu kompos pada skala 100 Kg sudah cukup baik, dengan nilai kapasitas ikat air yang lebih tinggi dari kompos yang dihasilkan pada skala laboratorium. Kata kunci:  evaluasi kinerja, co-composting, laju penurunan C/N, kondisi proses
SINTESIS NANOSILIKA DARI ABU KETEL INDUSTRI GULA DENGAN METODE ULTRASONIKASI DAN PENAMBAHAN SURFAKTAN Andes Ismayana, Akhiruddin Maddu, Illah Saillah, Ersyad Mafquh, Nastiti Siswi Indrasti
Jurnal Teknologi Industri Pertanian Vol. 27 No. 2 (2017): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

Boiler ash from sugar industry contains silica compounds which could be synthesized into nanosilica by ultrasonication method. The addition of surfactants in this synthesis method can affect the characteristics of nanosilica. This study aims to synthesize nanosilica with ultrasonication method and determine the effect of surfactants on characteristics of nanosilica generated. This research was divided into three stages: (1) boiler ash preparation to furnace ash, (2) silica extraction from furnace ash, and (3) synthesis of nanosilica using ultrasonication method with surfactant addition: PEG 6000 1:5, CMC 2.5% (b/b), CMC 5% (b/b), CMC 10% (b/b), and Tween 80 3% (b/v). Silica content in boiler ash and furnace ash was 49.69% and 78.75%, respectively. The nanosilics produced using the ultrasonication method had the uniform size (PDI) of 0.638, and with the addition of surfactant might increase the uniformity of the particle (PDI) to 0.045 and 0.047 on the usage of CMC 5 and 2.5%. The addition of surfactant also increased the crystallinity degree from 76.96% at no surfactant to 84.04% with 10% CMC surfactant added. The crystalline size of the silica nanoparticles also became smaller with the addition of 10% CMC of 37.69 nm from 41.40 nm crystal size without the addition of surfactant. The addition of an enlarged CMC concentration would increase the degree of crystallinity and decrease the crystal size of the nanosilica particles.Keywords : boiler ash, nanosilica, ultrasonication, surfactant, degree of crystallinity
NANOSILICA FROM THE BOILER ASH OF THE SUGAR CANE INDUSTRY AS A FILLER OF POLYSULFONE AND SULFONATED POLYETHERETHERKETONE MEMBRANES IN DIRECT METHANOL FUEL CELL Andes Ismayana, Akhiruddin Maddu, Yunia Istifani, Nastiti Siswi Indrasti
Jurnal Teknologi Industri Pertanian Vol. 28 No. 1 (2018): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

Nanosilika dari abu ketel industri gula tebu dapat digunakan sebagai pengisi untuk komposit polimer PSF-sPEEK untuk menjadi membran alternatif pada DMFC yang selama ini menggunakan membran elektrolit Politetrafloroetilena (PTFE).  Membran PTFE tersebut memiliki harga tinggi, methanol crossover tinggi, stabilitas rendah pada suhu tinggi, dan konduktivitas rendah pada kelembaban rendah atau suhu tinggi. Tujuan penelitian ini adalah untuk menentukan pengaruh dari komposisi perbandingan polisulfon (PSF)-polietereterketon tersulfonasi (sPEEK) dan perbandingan campuran polimer tersebut dengan penambahan nanosilika terhadap kinerja membran. Penelitan ini dilakukan melalui sintesis nanosilika dari abu ketel dengan metode ektraksi basa dan proses presipitasi, serta penambahan nanosilika tersebut terhadap komposit PSF-sPEEK untuk membentuk membran elektrolit melalui metode inversi fasa. Komposisi perbandingan PSF-sPEEK yaitu 1:9; 1:12; 1:15 sedangkan penambahan nanosilika yaitu sebanyak 1%, 3% dan 5%. Nanosilika yang dihasilkan memiliki ukuran partikel 330 nm, PDI (polydispersity index) 0.3, ukuran kristal 47,49 nm, dan derajat kristalinitas 64%. Uji ANOVA menunjukkan bahwa penambahan nanosilika dan komposisi PSF-sPEEK berpengaruh nyata terhadap kinerja membran. Membran dengan komposisi 1:12 dengan penambahan nanosilika 3% memiliki kinerja lebih baik dibandingkan membran yang lainnya dengan nilai daya serap air, daya serap methanol, kapasitas penukar ion dan konduktivitas ionic berturut-turut 27,21%, 25,62%, 2,56 meq/g, dan 66,28x10-6 S/cm. Kata kunci: membran DMFC, nanosilika, PSF (polisulfon), sPEEK (polieter-eterketontersulfonasi).
Co-Authors - - Nurrahman . Sugianto Abdulloh Rifai Agus Ghautsun Niam Ahmad Aminuddin Ahmad S. Sulaeman Andhika Prima Prasetyo Anita Firmanti Bagus MARTIANTO Bambang - Riyanto Bambang Herawan Bambang Herawan, Bambang Bambang Riyanto Bambang Sugeng Bonny Poernomo Wahyu Soekarno Candra A. Basuki Dede Hermawan Deni Shidqi Khaerudini Dimas Ramadhan Putra Dimas Ramadhan Putra Djoko Pujiarto Dodyk Pranowo Dyah Iswantini Erliza Noor Esa Ghanim Fadhallah Esa Ghanim Fadhallah Faozan Ahmad Farly Reynol Tumimomor Fayakun, Kun Fitriani Indahsari Gareso, Paulus P. Gareso, Paulus P. Gustan Pari Gustan Pari Gustan Pari Gustan Pari Hamdani Zain Hamdani Zain Hamdani Zain Hamdani Zain Hans Baihaqi Harry Ramza Harry Ramza Hendradi Hardhienata HENNY PURWANINGSIH HERA MAHESHWARI HERRY SUHARDIYANTO Horasdia Saragih Huda Salahuddin Darusman ikhwan wirahadikesuma Ikhwan Wirahadikesuma Inda Setyawati, Inda Ira Ukhtiningsih Ira Ukhtiningsih, Ira Irmansyah . Irmansyah Irmansyah Irmansyah Irmansyah Irmansyah Irmansyah Irzaman, Irzaman Ismail Budiman Ismail Budiman Isnaeni Isnaeni Isnaeni Isnaeni Isnaeni Jhav Sund Oktoricoento Johan D. Sirait Jufra Jufra Jumardin Jumardin Jumardin Jumardin Jumardin Kadapi, Muhamar Koekoeh Santoso Kudang Boro Seminar Kun Fayakun Kun Modjahidin Kun Modjahidin, Kun La Ode Muliadi LAKSMI AMBARSARI Liesbetini Haditjaroko Lina Karlinasari M. Farouq Iksan Mahfuddin Zuhri Mersi Kurniati Moehamad Barmawi Mohamad Rahmad Suhartanto Mohammad Mujirudin Muhammad F Ikhsan Muhammad Ramdani Mujirudin, Mohammad Novik Nurhidayat Nurfina Yudasari Nurul Rostami Dewi Nurul Rostami Dewi Oktarina Heriyani Oktarina Heriyani Oktoricoento, Jhav Sund Paulus P Gareso, Paulus P Pepen Arifin Permono Adi Putro Pramudito, Sidikrubadi Rahman, Mohammad Syuhaimi Ab Rahmaniah Nalwi Ramdani, Muhammad Ramza, Harry Rasyida Ulfa Ratna Sari Dewi Rini Kurniasih Rinto Paputungan Riza Riano Safaruddin A Prasad Sahrudin Sahrudin Sahrudin, Sahrudin Sar Sardy Sar Sardy Sar Sardy Setyanto Tri Wahyudi Siti Nikmatin Sofian, Irmansyah Sri Sugiarti Subyakto Subyakto SUBYAKTO SUBYAKTO Sugianto Arjo Sugianto, . Sugianto, . Sugianto, Sugianto Sulaeman, Ahmad Sofyan Sulistioso Giat Sukaryo Sultan, Syamsu Supian, Latifah Sarah Supriyanto . Tarcisius Rio Mardikanto Yayan Firmansyah Yessie Widya Sari Yogi Waldingga Hasnedi Yulia Irdawati Yulia Sani Yulia Sani, Yulia