Hanies Ambarsari
Institute of Environmental Technology (BTL), Indonesian Agency for Assessment and Application of Technology (BPPT), Building 412 PUSPIPTEK Serpong, Tangerang Selatan 15314, Indonesia

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Journal : Jurnal Energi dan Lingkungan (Enerlink)

ACETONE-BUTANOL-ETHANOL FERMENTATION FOR BIOENERGY USING VARIOUS SUBSTRATES IN DEFINED TYA MEDIA Ambarsari, Hanies; Sonomoto, Kenji
Jurnal Energi dan Lingkungan (Enerlink) Vol 11, No 1 (2015)
Publisher : Jurnal Energi dan Lingkungan (Enerlink)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5600.892 KB)

Abstract

A series of experiments had been conducted to investigate the performance of Clostridiumsaccharoperbutylacetonicum strain N1-4 (ATCC 13564) in direct fermentation of acetone, butanol, andethanol (ABE) using several substrates that have not been investigated previously using this strain,such as cellobiose, dextrin, starches of corn, potato, tapioca, wheat, and sago, as well as morecomplex carbohydrates such as xylans, cellulose, and carboxyl methyl cellulose (CMC), particularly tostudy the effect of such various substrate types and concentrations, as well as the substrates mixtureson the ABE ratio and productivity. The results showed that strain N1-4 could directly produce ABE fromvarious substrates, from monosaccharide to polysaccharides of hexose or pentose sugars, exceptCMC and cellulose. The experiments also suggested that cellobiose (a hexose disaccharide) could bethe more efficient substrate for strain N1-4 than glucose (a monosaccharide) to produce ABE in the TYA(Tryptone Yeast Agar) medium. It was proven also that the ABE ratio was very dependent on the typeand concentration of substrates being used by strain N1-4.Keywords: acetone, butanol, ethanol, clostridium saccharoperbutylacetonicum N1-4, fermentation
ACETONE-BUTANOL-ETHANOL FERMENTATION FOR BIOENERGY USING VARIOUS SUBSTRATES IN DEFINED TYA MEDIA Ambarsari, Hanies; Sonomoto, Kenji
Jurnal Energi dan Lingkungan (Enerlink) Vol 11, No 1 (2015)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5600.892 KB) | DOI: 10.29122/elk.v11i1.1590

Abstract

A series of experiments had been conducted to investigate the performance of Clostridiumsaccharoperbutylacetonicum strain N1-4 (ATCC 13564) in direct fermentation of acetone, butanol, andethanol (ABE) using several substrates that have not been investigated previously using this strain,such as cellobiose, dextrin, starches of corn, potato, tapioca, wheat, and sago, as well as morecomplex carbohydrates such as xylans, cellulose, and carboxyl methyl cellulose (CMC), particularly tostudy the effect of such various substrate types and concentrations, as well as the substrates mixtureson the ABE ratio and productivity. The results showed that strain N1-4 could directly produce ABE fromvarious substrates, from monosaccharide to polysaccharides of hexose or pentose sugars, exceptCMC and cellulose. The experiments also suggested that cellobiose (a hexose disaccharide) could bethe more efficient substrate for strain N1-4 than glucose (a monosaccharide) to produce ABE in the TYA(Tryptone Yeast Agar) medium. It was proven also that the ABE ratio was very dependent on the typeand concentration of substrates being used by strain N1-4.Keywords: acetone, butanol, ethanol, clostridium saccharoperbutylacetonicum N1-4, fermentation