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AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA , MSi., Dr. Muhtadi; Ambarwati, Ria; Yuliani, Ratna
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi� cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 μg/disk, 800 μg/disk, 1600 μg/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 μg/disk, 800 μg/ disk, 1600 μg/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, � avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra� .
EFEKTIFITAS PEMBERIAN JUS TOMAT DITAMBAH JERUK NIPIS TERHADAP TEKANAN DARAH PADA WANITA DEWASA Hidayah, Nur; Ambarwati, Ria
JURNAL RISET GIZI Vol 5, No 1 (2017): Mei (2017)
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrg.v5i1.4318

Abstract

Background  : Treatment of hypertension in the long term can affect adherence and cause toxic effects, therefore it takes alternative treatment by utilizing nutrients in food, one of which is the antioxidant like as lycopene and flavonoid in tomato-lime juice. Objective  : The aim of this study was to analyze the effect of tomato juicewith extra lime on blood pressure in women adults. Methods  : This study was clinical nutrition research with True Experiment randomized repeated measure control group design. The subject were 39 women adult 40-60 years, 19 control groups and 20 in treatment. Intervention was performed for 21 days with the measurement of systolic and diastolic blood pressure every 1 week 1 time. The statistical analysis was done by Anova Repeated Measure test with 95% confidence degree. Results  : Anova Repeated Measure show that tomato juice with extra limecan lowered systolic and diastolic blood pressure (α = 0.005, p = 0.002 and p = 0.038), while the level of energy, protein, fat, carbohydrate, fiber, sodium, and potassium did not affect in systolic and diastolic blood pressure. Conclusion  : tomato-lime juice effectively lowered systolic and diastolic blood pressure.
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN FRAKSI KULIT BATANG BELIMBING WULUH (Averrhoa bilimbi Linn.) TERHADAP BAKTERI Klebsiella pneumoniae DAN Staphylococcus epidermidis BESERTA BIOAUTOGRAFINYA , MSi., Dr. Muhtadi; Ambarwati, Ria; Yuliani, Ratna
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v4i2.252

Abstract

Belimbing wuluh (Averrhoa bilimbi Linn.) is a tropical plant that has antibacterial properties. The purpose of this study was to test the antibacterial activity of bark Belimbing wuluh against Klebsiella pneumoniae and Staphylococcus epidermidis and their bioautography. Extraction methods used to research is method maceration with a solvent ethanol 96 %. Fractinations done by method partition liquid-liquid with a separating funnel. Test performed in this research covering identi? cation bacteria, the sensitivity bacteria, antibacterial activity, thin layer chromatography, bioautography. The result of antibacterial activity ethanol extract of disk diffusion method with concentrations 400 ?g/disk, 800 ?g/disk, 1600 ?g/disk is 8±0,5; 10,34±0,58; 12,17±0,76 on Klebsiella pneumoniae, 10,17±0,29; 11±0; 11.5±0 on Staphylococcus epidermidis, n-hexane fraction with concentration 400 ?g/disk, 800 ?g/disk, 1600 ?g/disk is 8,34±0,29; 9,34±0,29; 10,84±0,76 on Klebsialla pneumoniae, 8,5±0,5; 9,34±0,29; 10,67±0,29 on Staphylococcus epidermidis, ethyl acetate fraction with concentration 400 ?g/disk, 800 ?g/disk, 1600 ?g/disk is 9,17±0,29; 10,34±0,29; 11,17±0,29 on Klebsiella pneumoniae and 9,5±0,5; 10,67±0,29; 12,67±1,26 on Staphylococcus epidermidis, ethanol-water fractions with concentration 400 ?g/disk, 800 ?g/ disk, 1600 ?g/disk is 8,17±0,29; 9,17±0,29; 10±0 on Klebsiella pneumoniae, 9±0; 9,67±0,29; 10,34±0,29 on Staphylococcus epidermidis. The TLC show chemical compounds contained in the ethanol extract, n-heksan fraction, ethyl acetate fraction, and ethanol-water fraction is a compound of the saponins, alkaloids, ? avonoids and phenolic. Bioautography showed that ethanol extracts, n-heksan faction, ethyl acetate fraction, and etanol-airfaction Belimbing wuluh (Averrhoa bilimbi Linn.) bark have not antibacterial activity because there is no clear area around on plate TLC.Keywords: Belimbing wuluh (Averrhoa bilimbi Linn.), ethanol extract, fractination, antibacterial, bioautogra? .
Pengaruh konseling laktasi intensif terhadap pemberian air susu ibu (ASI) eksklusif sampai 3 bulan Ambarwati, Ria; Muis, Siti Fatimah; Susanti, Purwanti
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 2, No 1 (2013)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.915 KB) | DOI: 10.14710/jgi.2.1.

Abstract

Background : In 2010 within the area of Semarang Municipality, exclusive breastfeeding by mothers to their babies reaches  up to only 20,06%. One of the problems was the lack of lactation counseling especially by the health service institutions. Objectives: To reveal the effect of intensive lactation counseling on exclusive breastfeeding up to 3 months. Method: This research study was designed as a quasi-experiment of non-equivalent control group. The population of this study was a group of mothers who were in the 7th to 8th months of pregnancy bearing the second child or more. The number of the subjects was 25 mothers who were grouped into 2: the experimental groups of 12(n=12) and 27 control group of 13 (n=13). The group underwent a treatment of intensive lactation counseling of 9 times on 2 stages, the first was four-time treatments during the medical examination on their pregnancy of 7th – 8th months. The second stage of treatments was done by home visit for 5 times during week 1, 2, 4, 8, and 12 after giving birth by certified lactation counselors for 40 work hours. These counselors had the experience of at least 5 clients counseling. The control group had the usual counseling that they normally received from the midwives at the Health Primary Center and  private midwives nearby. Results: The group without intensive lactation counseling showed no gain of knowledge, attitude towards early initiation of breastfeeding, exclusive breastfeeding, breastmilk and no increase of the number of exclusive breastfeeding on their babies prior to and during the study. The group with intensive lactation breastfeeding shows a significant gain of knowledge, positive attitude towards early initiation of breastfeeding, exclusive breastfeeding, breastmilk and significant increase of the number of exclusive breastfeeding for 3 months on their babies that born prior to and during the study from 2(16,7%) to 10(83,3%). Conclusion: Intensive lactation counseling increases the practice of exclusive breastfeeding up to 3 months. Keywords : intensive lactation counseling, exclusive lactation breastfeeding, 3 months
Efektifitas Aktivitas Peer Group terhadap Penurunan Berat Badan dan Persen Lemak Tubuh pada Remaja Overweight Jaelani, Mohammad; Larasati, Meirina Dwi; Rahmawati, Ana Yuliah; Ambarwati, Ria
Jurnal Kedokteran Brawijaya Vol 30, No 2 (2018)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.721 KB) | DOI: 10.21776/ub.jkb.2018.030.02.9

Abstract

Diet rendah energi dan peningkatan aktivitas secara individual belum efektif dalam penurunan berat badan. Oleh karena itu, perlu dicari metode lain atau memodifikasi metode dengan membentuk kelompok peer group aktivitas. Tujuan penelitian ini menganalisis efektifitas aktivitas peer group terhadap kepatuhan aktivitas fisik, kepatuhan diet, penurunan berat badan dan persen lemak tubuh pada remaja overweight. Desain penelitian menggunakan Randomized Controlled Trial dengan rancangan eksperimental ulang pre-posttest control group design. Subjek penelitian adalah remaja putri overweight sebanyak 13 orang kelompok kontrol dan 13 orang kelompok perlakuan. Subjek dilakukan intervensi berupa jogging minimal 30 menit dan naik turun tangga sebanyak 10 kali per hari selama empat minggu. Ukuran kepatuhan diet diambil setiap minggu berdasarkan hasil recall 2x24 jam selama 4 minggu. Perbedaan kepatuhan aktivitas fisik dan kepatuhan diet diuji menggunakan Chi Square Test sedangkan perubahan berat badan dan persen lemak tubuh menggunakan ANOVA Repeated Measure Test. Ada perbedaan yang signifikan antara kepatuhan aktivitas fisik (p=0,000) dan penurunan berat badan (p=0,004) antara kelompok perlakuan dan kontrol namun tidak ada perbedaan yang signifikan antara kepatuhan diet (p>0,05) dan penurunan persen lemak tubuh (p=0,382) antara kelompok perlakuan dan kontrol. Peer group efektif dalam meningkatkan aktivitas fisik dan menurunkan berat badan pada remaja overweight.
PENGEMBANGAN MAKANAN TAMBAHAN BERBASIS F100 DENGAN SUBSTITUSI TEPUNG LABU KUNING DAN TEPUNG PISANG Ambarwati, Ria
Journal of Nutrition College Vol 9, No 2 (2020): April
Publisher : Departemen Ilmu Gizi, Fakultas Kedokteran, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.255 KB) | DOI: 10.14710/jnc.v9i2.27033

Abstract

Latar Belakang: Perlu pengembangan modifikasi makanan tambahan dengan komposisi bahan berbasis F100.Tujuan: Mengetahui perbedaan kadar energi, protein dan lemak serta uji daya terima cookies berbasis F100 dengan substitusi tepung labu kuning dan tepung pisang.Metode: Jenis penelitian ini adalah penelitian eksperimen rancangan acak lengkap 1 faktorial. Konsentrasi substitusi tepung labu kuning dan tepung pisang 10%, 20%, 30% dan 0% sebagai kontrol dengan 3 kali ulangan. Kadar energi dengan menggunakan DKBM, kadar protein diuji dengan metode micro Kjeldahl dan kadar lemak dengan metode soxlet. Uji daya terima pada 25 panelis agak terlatih dan 20 balita usia 2-5 tahun. Perbedaan kadar protein dan lemak diuji dengan ANOVA dan uji lanjut LSD, Tukey HSD. Uji daya terima panelis agak terlatih diuji dengan Friedman. Perbedaan kadar energi dan uji daya terima pada balita dianalisis secara deskriptif.. Hasil: Kadar energi paling tinggi pada cookies dengan subtitusi tepung labu kuning konsentrasi 10% (100,73 kkal/100 gram) dan tepung pisang konsentrasi 10% (101,23/100 gram). Ada perbedaan kadar protein dan lemak cookies dengan substitusi tepung labu kuning (p=0,000) dan substitusi tepung pisang (p=0,000). Ada perbedaan daya terima panelis terhadap rasa (p=0,046), warna (p=0,000), tekstur (p=0,007) dan tidak ada perbedaan aroma (p=0,126) cookies substitusi tepung labu kuning. Tidak perbedaan terhadap rasa (p=0,984), warna (p=0,352), tekstur (p=0,758), aroma (p=0,680) cookies substitusi tepung pisang. Lebih dari 50% balita menghabiskan cookies substitusi tepung labu kuning konsentrasi 10%, 20% dan tepung pisang konsentrasi 30%.Kesimpulan: Konsentrasi substitusi tepung labu kuning 10%, 20% dan substitusi tepung pisang 30% dapat direkomendasikan sebagai alternatif makanan tambahan.1.    Nency Y, Arifin MT. Gizi Buruk, ancaman generasi yang hilang. 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Jakarta: Direktorat Pembinaan Sekolah Menengah Kejuruan; 2008.p 496–515.9.    Sutomo BD. Sukses Wirausaha Kue Kering. Cetakan V. Jakarta: Kriya Pustaka; 2012. p.18. 10. Hendrasty, Krissetiana H. Tepung Labu Kuning Pembuatan dan Pemanfaatannya. Yogyakarta: Kanisius; 2003. p. 9. 11. Azhariati R. Pengaruh metode pengeringan terhadap kerusakan betakaroten mi ubi kayu yang diperkaya tepung labu kuning. Agritech. 2008;22(4):153–7. 12. Masli R. Studi pembuatan tepung pisang kepok (musa paradisiaca forma typical) sebagai bahan substitusi pembuatan roti tawar (kajian tingkat kematangan pisang kepok dan suhu pengeringan). Departemen Agroindustri Universitas Muhammadiyah Malang. Skripsi. 2010; 13. Karlin R, Rahayuni A. Potensi Yogurt Tanpa Lemak Dengan Penambahan Tepung Pisang Dan Tepung Gembili Sebagai Alternatif Menurunkan Kolesterol. Journal of Nutrition College. 2014;3(2):293–302. 14. Soekarto ST. Penelitian Organoleptik untuk Industri Pangan dan Hasil Pertanian. Jakarta: Bhatara Karya Aksara; 1985. p. 1–121.15. Persatuan Ahli Gizi Indonesia. Daftar Komposisi Bahan Makanan. Jakarta. Persatuan Ahli GiziIndonesia; 2005. 16. Persatuan Ahli Gizi Indonesia. Tabel Komposisi Pangan Indonesia. Jakarta: Elex Media Komputindo; 2009. 17. Riganakos KA, Kontominas MG. Effect of heat treatment on moisture sorption behavior of wheat flours using a hygrometric tehnique. Developments in Food Science. 1995;37:995–1005. 18. See EF, Wan Nadiah WA, Noor Aziah AA. Physico-chemical and sensory evaluation of breads supplemented with pumpkin flour. ASEAN Food Journal. 2007;14(2):123–30. 19. Asmaraningtyas D. Kekerasan, warna dan daya terima biskuit yang disubstitusi tepung labu kuning. Universitas Muhammadiyah Surakarta. Skripsi. 2014.20. Winarno F. Kimia Pangan dan Gizi. Jakarta: Gramedia Pustaka Utama; 2004. p 41–43.21. Utomo LIVA, Nurali E, Ludong M. Pengaruh Penambahan maizena pada Pembuatan Biskuit Gluten Free casein Berbahan Baku Tepung Pisang Goroho (Musa Acuminate). Cocos, 2017;1(2).22. Subandoro RH, Basito, Atmaka W. Pemanfaatan tepung millet kuning dan tepung ubi jalar kuning sebagai subtitusitepung terigu dalam pembuatan cookies terhadap karakteristik organoleptik dan fisikokimia. Jurnal Teknosains Pangan. 2013;2(4):68–74. 23. Yasinta UNA, Dwiloka B. Nurwantoro N. Pengaruh subtitusi tepung terigu dengan tepung pisang terhadap sifat fisikokima dan organoleptik cookies. Jurnal Aplikasi Teknologi Pangan. 2017;6(3):119–21. 24. Sitohang KAK, Lubis Z, Lubis LM. Pengaruh perbandingan jumlah tepung terigu dan tepung sukun dengan jenis penstabil terhadap mutu cookies sukun. Jurnal Rekayasa Pangan dan Pertanian. 2015;3(3):308–15. 25. Lolodatu ES. Purwijatingngsih LME, Pranata F. Kualitas non flaky crackers coklat dengan variasi substitusi tepung pisang kepok kuning (musa paradisiaca forma typica). Jurnal Teknobiologi. 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PENGARUH EDUKASI GIZI DENGAN MEDIA PUZZLE TERHADAP PENGETAHUAN DAN SIKAP TENTANG SAYUR DAN BUAH PADA ANAK SEKOLAH DASAR Safitri, Yeni Liza; Sulistyowati, Enik; Ambarwati, Ria
Journal of Nutrition College Vol 10, No 2 (2021): April
Publisher : Departemen Ilmu Gizi, Fakultas Kedokteran, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jnc.v10i2.29139

Abstract

Background: Consumption of vegetables and fruit among school age children is still low due to lack of knowledge and attitudes about vegetables and fruit. Nutrition education can be provided to increase knowledge and attitudes as early as possible. Nutrition education using puzzle media is expected to increase knowledge and form a positive attitude about vegetable and fruit consumption.Objective: To determine the effect of nutrition education using puzzle media towards knowledge and attitudes about vegetables and fruit in elementary school children.Methods: This was a quasi-experimental study with pretest-posttest control group design. Fourty elementary school children were divided into two groups. The treatment group received nutrition education using puzzle media and a control group received nutrition education without puzzle media. Knowledge and attitudes were assessed before and after the study based on scores. Data were analyzed using Paired T-Test and Independent Sample T-Test.Results: The average score od knowledge in the treatment group increase by 18.25 points and the control group scre increased by 12.25 points. The mean score of attitudes in the treatment group increased by 14.45 points and the control group (p=0.014) and the attitude scores between the treatment group and the control group (p=0.003)Conclusion: Nutrition education using puzzle media can increase knowledge and attitudes about vegetables and fruit in elementary school children.Keywords: Nutrition education; Puzzle media; Vegetable and fruit; Knowledge; Attitude.