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All Journal Prosiding Seminar Biologi ComTech: Computer, Mathematics and Engineering Applications Proceeding Biology Education Conference
Wiwit Amrinola
Research Centre for Biotechnology, LIPI. Jl. Raya Bogor Km 46, Kec. Cibinong, Kab. Bogor, 16911. Indonesia. Telp: +62-21-8754587.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health

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DETEKSI ENZIM CELLOBIOSE DEHYDROGENASE (CDH) DARI FUNGI TRAMETES VERSICOLOR Kusumawati, Dwi Endah; Amrinola, Wiwit; Hasanah, Neneng; Desriani, Desriani; Falah, Syamsul; Bintang, Maria
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.247 KB)

Abstract

Trametes versicolor merupakan salah satu fungi pendegradasi kayu dari golongan white rot fungi yang memiliki kemampuan dalam menghasilkan enzim ekstraseluler, yaitu cellobiose dehydrogenase (CDH). Enzim CDH memiliki dua domain, yaitu domain flavin dan domain heme yang dihubungkan oleh 25 asam amino yang kaya akan serin dan treonin. Penelitian ini bertujuan mendeteksi CDH yang dihasilkan oleh T. versicolor baik secara kualitatif maupun kuantitatif. Deteksi CDH dilakukan melalui empat  tahap, yaitu: uji kualitatif enzim ekstraseluler, produksi CDH pada media cair, pengukuran aktivitas CDH menggunakan DCIP sebagai akseptor elektron dan selobiosa sebagai substrat, serta analisis bobot molekul CDH dengan elektroforesis SDS-Page. Hasil penelitian menunjukkan terbentuknya zona bening disekitar fungi setelah perlakuan congo red dan NaCl yang mengindikasikan bahwa T. versicolor menghasilkan enzim ekstraseluler. Produksi CDH dilakukan dengan menumbuhkan fungi di media YPD dan media minimal guna menginduksi produksi CDH. Pengukuran aktivitas enzim CDH belum berhasil dilakukan dan berdasarkan hasil SDS-PAGE, sebagian besar enzim CDH dari T. versicolor dihasilkan dalam bentuk domain flavin.  Kata kunci : Cellobiose dehydrogenase, SDS-Page, Trametes versicolor
Metode Pembuatan Sorgum Sosoh Rendah Tanin pada Pembuatan Nasi Sorgum (Sorghum Bicolor L) Instan Widowati, Sri; Hariyadi, Purwiyatno; Amrinola, Wiwit
ComTech: Computer, Mathematics and Engineering Applications Vol 6, No 1 (2015): ComTech
Publisher : Bina Nusantara University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21512/comtech.v6i1.2280

Abstract

Sorghum is one of the non-rice commodities which have a relatively high content of nutrients, especially protein and carbohydrate. However, the nutritional value is to be down and relatively low due to the relatively high tannin content as an anti-nutrient. Fairly high tannin content in sorghum is also causing sorghumhas unpleasant taste and slightly bitter or "Sepet". Therefore, it is necessary to reduce the content of tannins in sorghum that is expected to improve the quality of nutrition, especially the increase in protein and starch digestibility and palatability or the flavor of sorghum products. The purpose of this study is to obtain the best method to lower tannin content in the manufacture of low-tannin sorghum milling, which will be used in the manufacture of instant rice sorghum. This research was done in two stages, namely 1) the timing of the millingstage and 2) the development stage of a method of making low-tannin sorghum milling by comparing the content of tannin reduction method with the immersion process in alkaline solution (NaOH 0.3% and 0.3% Na2CO3) and distilled water with the method of reducing tannin content without immersion process. The results of this study indicate that the best milling time that can produce milled sorghum with good physical sorghum is five minutes and the best immersion treatment that can lower the optimum tannin content is by immersing in 0.3%Na2CO3 solution for 24 hours. This method can reduce up to 77.46% tannin content.
Survey Instrument Development for Consumer Perception Testing to RTE Product in Convenience Store Widaningrum, Dyah Lestari; Amrinola, Wiwit; Nasution, Fuadi
ComTech: Computer, Mathematics and Engineering Applications Vol 6, No 3 (2015): ComTech
Publisher : Bina Nusantara University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21512/comtech.v6i3.2214

Abstract

Global trade and the abolition of trade barriers lead to the proliferation of imported food products such as various types of fast food products (RTE: ready-to-eat food) sold by convenience stores mainly scattered in Jakarta. The question is what causes consumers choose certain convenience store to buy RTE Product and not choose another outlet category. To understand consumer perceptions, it is necessary to develop an instrument for measuring consumer perceptions of the option to purchase RTE Product at convenience stores. Variables used in this research are the perception and knowledge of the public/consumers against food safety, store attributes, service, and product attributes. The instrument used in this study is a questionnaire with a scalemeasuring1-5 to see the relationship between variables, we used the method of Structural Equation Modeling (SEM). The sample used in this study was 223 respondents that were divided into five areas in Jakarta, namely Central Jakarta, West Jakarta, South Jakarta, East Jakarta, and North Jakarta. The final outcome of the instrument development process was 25 observed variables, consisting of 4 indicators, which valid and reliable, with T-values for each observed variable is above 2,58, and Construct Reliability values range between 0,76 to 0,853.
DETEKSI ENZIM CELLOBIOSE DEHYDROGENASE (CDH) DARI FUNGI Trametes versicolor Desriani, Desriani; Falah, Syamsul; Bintang, Maria; Kusumawati, Dwi Endah; Amrinola, Wiwit; Hasanah, Neneng
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Trametes versicolor merupakan salah satu fungi pendegradasi kayu dari golongan white rot fungi yang memiliki kemampuan dalam menghasilkan enzim ekstraseluler, yaitu cellobiose dehydrogenase (CDH). Enzim CDH memiliki dua domain, yaitu domain flavin dan domain heme yang dihubungkan oleh 25 asam amino yang kaya akan serin dan treonin. Penelitian ini bertujuan mendeteksi CDH yang dihasilkan oleh T. versicolor baik secara kualitatif maupun kuantitatif. Deteksi CDH dilakukan melalui empat  tahap, yaitu: uji kualitatif enzim ekstraseluler, produksi CDH pada media cair, pengukuran aktivitas CDH menggunakan DCIP sebagai akseptor elektron dan selobiosa sebagai substrat, serta analisis bobot molekul CDH dengan elektroforesis SDS-Page. Hasil penelitian menunjukkan terbentuknya zona bening disekitar fungi setelah perlakuan congo red dan NaCl yang mengindikasikan bahwa T. versicolor menghasilkan enzim ekstraseluler. Produksi CDH dilakukan dengan menumbuhkan fungi di media YPD dan media minimal guna menginduksi produksi CDH. Pengukuran aktivitas enzim CDH belum berhasil dilakukan dan berdasarkan hasil SDS-PAGE, sebagian besar enzim CDH dari T. versicolor dihasilkan dalam bentuk domain flavin.  Kata kunci : Cellobiose dehydrogenase, SDS-Page, Trametes versicolor
Co-Authors A.A. Ketut Agung Cahyawan W Desriani Desriani Dwi Endah Kusumawati Dyah Lestari Widaningrum MARIA BINTANG Nasution, Fuadi Neneng Hasanah Purwiyatno Hariyadi Siti Zubaidah Syamsul Falah