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All Journal HAYATI Journal of Biosciences CHEMISTRY PROGRESS Jurnal Agro Teknologi Tropika Indonesian Journal of Agricultural Science Jurnal Sumberdaya Lahan Jurnal Sumberdaya Lahan
Iswandi Anas
Departemen Ilmu Tanah dan Sumberdaya Lahan Fakultas Pertanian Insttut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Chemistry Earth & Planetary Sciences

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Journal : HAYATI%20Journal%20of%20Biosciences

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Isolation, Selection and Identification of Polyaromatic Hydrocarbons (PAHs) Degrading Bacteria from Heavy Oil Waste (HOW)-Contaminated Soil Yani, Mohamad; Charlena, Charlena; Mas?ud, Zainal Alim; Anas, Iswandi; Setiadi, Yadi; Syakti, Agung Dhamar
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.935 KB) | DOI: 10.4308/hjb.27.2.142

Abstract

The heavy oil waste (HOW) containing polyaromatic hydrocarbon (PAHs) is a persistent organic pollutants (POPs) that difficult to degrade. The new PAH degrading consortium was investigated from HOW contaminated soil in North Sumatera of Indonesia. The isolation, selection and identification of polyaromatic hydrocarbon degrading bacteria from soil contaminated by HOW was conducted to solve a bioremediation process. The isolation microbes from soil contaminated by HOW was performed using a minimum ONR7a media and followed on marine agar media for purification purposes. From the performed isolation results, 11 isolates were able to degrade PAHs compounds, such as phenanthrene, dibenzothiophene, or fluorene compounds. They grew at pH range of 4.8-8.2 and performed on emulsification activity in paraffin from 0.150-0.662. Three of them showed the best performance on HOW biodegradation capability and then successfully selected and identified as Salipiger sp., Bacillus altitudinis, and Ochrobactrum anthropi. using 16S rDNA. The HOW biodegradation as TPH-degradation were 38.66%, 59.60%, and 47.16%, respectively. Those isolated bacteria could potentially be as bioremediation agents to develop on bioremediation process for soils contaminated by HOW.
Co-Authors Agung Dhamar Syakti Anak Agung Istri Sri Wiadnyani Arum Asiyanti Suhastyo, Arum Asiyanti Atang Sutandi Bariot Hafifa Charlena DWI ANDREAS SANTOSA Edi Santosa Mas?ud, Zainal Alim Moh. Yani Nelvia Nelvia SUBOWO GITOSUWONDO Suprihatin, Mohammad Yani, dan Dewi Ratnasari Suyamto Suyamto Yadi Setiadi YULIN LESTARI