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The quantity of neutrofil and makrophage after the application of red ginger on white rats with chronic periodontitis Andayani, Ridha; Chismirina, Santi; Pratiwi, Hessyi Amanda; Husni, Melati Hayatul
Padjadjaran Journal of Dentistry Vol 28, No 2 (2016): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.739 KB) | DOI: 10.24198/pjd.vol28no2.13721

Abstract

Introduction: Chronic periodontitis is the most common form of periodontitis. The major etiology of periodontitis is gram-negative anaerobic bacteria, such as Aggregatibacter actinomycetemcomitans, Treponema denticola, Tannarela forsythia and Porphyromonas gingivalis which locally invade periodontal tissues and affect the immune system cells. Neutrophils and macrophage play central role in inflammation. The objective of this study was to evaluate the quantity of neutrophil and macrophage due to the application of red ginger (Zingiber officinale var rubrum) extract of rat chronic periodontitis. Methods: The type of this research was experimental laboratoris post test only control group. In order to induce experimental periodontitis, the mandibular insisivus was assigned to receive wire 0,008 inch in marginal gingival. Then, red ginger (Zingiber officinale var rubrum) extract that has bioactive compounds was topically administered to the rats. Results: This study showed there were no neutrophils in histopathologic of rat’s periodontal tissue, bu there were amount of macrophage. Then macrophage analyzed by one way ANOVA showed significant differrences in each group (P<0,05) and continued with LSD. Conclusions: there was no effect of red ginger (Zingiber officinale var rubrum) extract toward the quantity of neutrophils, but affected on the number of macrophage.
Comparison of Candida albicans colony amount in heat-cured acrylic and thermoplastic nylon resin after immersion in Ulee Kareng coffee (Coffea robusta) Sundari, Iin; Andayani, Ridha; Harahap, Novriyanti Fatimah
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.041 KB) | DOI: 10.24198/pjd.vol29no1.11970

Abstract

Introduction: Heat-cured acrylic resin is the most often used material in the manufacture of denture base. Along with the development of science and technology, to overcome the shortcomings of heat-cured acrylic resin, repairment of denture base material was done, one of them is a thermoplastic nylon resin. On the use of denture often found Candida albicans attached to the denture. This study aimed to determine the comparison of the amount of Candida albicans colony on heat-cured acrylic and thermoplastic nylon resin after immersed in the Ulee Kareng coffee (Coffea robusta). Methods: The number of specimens in this study were 8 specimens; 4 Meliodent® heat-cured acrylic resins and 4 Bio Tone® thermoplastic nylon resins with size of 10x10x2 mm. The methods of this study was experimental laboratory. The specimens were stored in a solution of Ulee Kareng coffee (Coffea robusta) for 7 days. Each specimen was contaminated with Candida albicans, then the number of Candida albicans colony was counted with Colony counter, from threshing results of heat-cured acrylic and thermoplastic nylon resins. Data were analyzed with unpaired t test. Results: Unpaired t test results showed that there were significant differences (p<0.05) between the number of colonies of Candida albicans in heat-cured acrylic resin (4.5 CFU/ml) and thermoplastic nylon resin (1.5 CFU/ml) after both immersed in the Ulee Kareng coffee (Coffea robusta). Conclusion: The amount of Candida albicans colony on heat-cured acrylic resin was higher than on thermoplastic nylon resin.
The Antibacterial Effects of Syzygium Cumini Fruit Mouthwash Against Streptococcus mutans as an Agent of Dental Caries Chismirina, Santi; Andayani, Ridha; Sungkar, Suzanna
International Conference on Multidisciplinary Research Vol 3, No 1 (2020): ICMR
Publisher : Universitas Serambi Mekkah

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.714 KB) | DOI: 10.32672/pic-mr.v3i1.2597

Abstract

AbstractCaries is a disease of the oral cavity characterized by tooth decay due to the dissolution of minerals on the teeth by Streptococcus mutans (S. mutans) attached to the teeth surface. One way to prevent the bacteria from decaying the teeth is by gargling with a mouthwash containing antibacterial ingredients from herbs. The results of our previous research on the Syzygium cumini fruit showed that it was one of the natural ingredients with antibacterial compounds against S. mutans. This study aimed to determine the antibacterialeffects of the Syzygium cumini fruit which was formulated in the form of mouthwash against S. mutans. Syzygium cumini fruit was made into mouthwash preparations which were then tested for stability, including for the organoleptic and antibacterial tests. The results of the organoleptic test showed that the Syzigium cumini fruit mouthwash with formula 4 was the best choice for the mouthwash formula. Antibacterial test results showed that the Syzigium cumini fruit could inhibit the growth of S. mutans, characterized by the formation of a hallo zone with an average diameter of 8 mm. This diameter indicated that the Syzygium cumini fruit mouthwash had moderate inhibition against S. mutans.Keywords: Syzygium cumini, mouthwash, Streptococcus mutans, dental caries
PERBEDAAN LAJU ALIRAN SALIVA TERSTIMULASI ANTARA PENGUNYAHAN PARAFIN WAX DENGAN PERMEN KARET XYLITOL PADA PASIEN TERINDIKASI GERD Ridha Andayani; Sunnati .; Amatu Sholiha
ODONTO : Dental Journal Vol 3, No 2 (2016): December 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.3.2.105-110

Abstract

Background: Gastroesophageal Reflux Disease (GERD) is a condition of irreversibility (reflux) of stomach contents into the esophagus exceeds the normal amount and causes a variety of complaints. Reduction in salivary flow and decreased secretion of bicarbonate in the saliva cannot be avoided so that the patient is symptomatic GERD in the oral cavity in the form of halitosis and tooth erosion. Paraffin wax and chewing xylitol gum can be an alternative to increase the rate of saliva and neutralize the acid in patients with GERD. This study aimsto observes salivary flow rate differences between paraffin wax and xylitol gum patient - indicated GERD at RSUDZA in Banda Aceh.Method: This research is an analytic study with cross sectional approach and a subject of 17 people. Data were analyzed by statistical tests using Wilcoxon test.The results of this study, the salivary flows by chewing paraffin wax at the low category (52.9%), whereas salivary flows by chewing xylitol gum is the normal category (76.5%).Result: The data obtained P = 0.001 (P <0.05) for the difference between chewing paraffin wax and chewing xylitol gum.Conclusion: The conclusion from this study is a significant difference between salivary flows chewing paraffin wax and chewing xylitol gum in patient-indicated GERD at RSUDZA in Banda Aceh
POTENSI ANTIBAKTERI EKSTRAK JAHE (Zingiber officinale Roscoe) TERHADAP Porphyromonas gingivalis SECARA IN VITRO Zulfan M. Alibasyah; Ridha Andayani; Ana Farhana
Journal of Syiah Kuala Dentistry Society Vol 1, No 2 (2016): JULY
Publisher : Dentistry Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.499 KB)

Abstract

AbstractChronic periodontitis is the most common periodontal disease occurs in people with prevalence of96.58% and usually occurs at the age of 35 years and over. The main cause of chronic periodontitis isaccumulation of plaque and bacteria. The most dominant bacteria in chronic periodontitis isPorphyromonas gingivalis. Ginger (Zingiber officinale Roscoe) is a spice plant that is widely used bythe public, contain secondary metabolites such as flavonoids, terpenoids, phenols, and essential oilwhich is antibacterial. This study aim was to determined the ginger’s extract (Zingiber officinaleRoscoe) antibacterial potency against Porphyoromonas gingivalis with various concentrations are6.25%, 12.5%, 25%, 50%, and 100%. Antibacterial potency which test done by placing a paper discsthat has been dipped in the test group with 5 concentration and the placed on the surface of the MHAmedium (Mueller Hilton Agar) which had been grown Porphyromonas gingivalis, and incubated for 24hours at a temperature of 37 0C in an anaerobic atmosphere. Furthermore, inhibition zones werecalculated using calipers. Data were analyzed by one way ANOVA test which showed that the extractof ginger (Zingiber officinale Roscoe) can inhibit Porphyromonas gingivalis with probability (P0.05).Conclusion the extract of ginger (Zingiber officinale Roscoe) cold inhibit the growth ofPorphyromonas gingivalis at 6,25% concentration of extract with average of inhibition zone 10,6 mmthat categorized weak.Keywords: Ginger, Porphyromonas gingivalis, Chronic Periodontitis
PENGARUH KONSUMSI AIR MINUM REVERSE OSMOSIS (RO) TERHADAP LAJU ALIRAN, pH, DAN VISKOSITAS SALIVA PADA SISWA SMA NEGERI 10 FAJAR HARAPAN BANDA ACEH Santi Chismirina; Ridha Andayani; Afrina .; Putri Humaira Nanda Ibrahim; Hafizh Gunawan Amri
Journal of Syiah Kuala Dentistry Society Vol 4, No 1 (2019): OKTOBER
Publisher : Dentistry Faculty

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Abstract

One drink that is processed by the reverse osmosis method is Reverse Osmosis (RO) drinking water.Through this method, all micro particles contained in filtered water including essential minerals. Thereduction in essential minerals can reduce the activation function of salivary gland secretory cellsand it will have an impact on salivary flow, pH, and viscosity. This study aims to determine the effectof RO drinking water consumption to the flow rate, pH, and viscosity of saliva on students of FajarHarapan Senior High School Banda Aceh. The study subjects consisted of 45 subjects who consumedRO drinking water as the treatment group and 8 subjects who did not consume RO drinking water asa control group. Saliva from the treatment and control group subjects was collected by spittingmethod to measure the flow rate, pH, and viscosity of saliva. The results of the study that wereanalyzed using the unpaired T test showed that there was no effect of RO drinking water consumptionon the flow rate, pH, and viscosity of saliva in SMA Negeri 10 Fajar Harapan Banda Aceh. Based onthe results of the research, it can be concluded that the consumption of RO drinking water does notaffect the flow rate, pH and viscosity of the saliva in students of Fajar Harapan Senior High SchoolBanda Aceh. Keywords : Essential minerals, Calcium, Magnesium, Spitting method, pH meter.
EFEKTIVITAS SIFAT BAKTERIOSTATIK Porphyromonas Gingivalis DAN Lactobacillus Acidophilus SEBAGAI KONTROL BIOLOGI PERTUMBUHAN Candida Albicans DALAM BERBAGAI PH SALIVA BUATAN Basri A. Gani; Abdillah Imron Nasution; Ridha Andayani; Vivi Zayanti; Ratih Asrina Fitri
Journal of Syiah Kuala Dentistry Society Vol 1, No 2 (2016): JULY
Publisher : Dentistry Faculty

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Abstract

AbstractOral candidiasis caused by Candida albicans as a result of an imbalance of oral biology activities, preventive therapy with using chemical, biological as well one of them to increasing the effectiveness of the role of the bacteriostatic properties of normal oral flora such as Lactobacillus species and Porphyromonas species that helps maintain the regulation of carbohydrate fermentation activity of saliva in the form acid and alkaline pH. The purpose of this study evaluated the effectiveness of the bacteriostatic properties of P. gingivalis and L. Acidophilus in influencing the growth of C. albicans. While the materials used in this study are C. albicans and L. acidophilus strains of the laboratory, P. gingivalis ATCC 33 277 and artificial saliva. The results obtained by the method of SDA and NA media culture, pH saliva test interactions, the calculation of the bacteria colony by colony counter, and a slide culture for C. albicans. The results showed a change leads to an alkaline pH of saliva after interacted by C. albicans with P. gingivalis (p 0.05) and C. albicans with L. acidophilus (p 0.05) using pH control of 4,5,6,7,8, and 9. Further, the colony of P. gingivalis growth is more dominant compared to C. albicans (p 0.05), but on the contrary, C. albicans colonies growth was more dominant than the L. acidophilus (p 0.05). Nevertheless, the those bacteria are capable of inhibiting the growth of hypha from C. albicans as a virulence factor that most affects the host mucosal infection. From the research results can be concluded that the interaction of C. albicans, P. gingivalis and L. acidophilus in artificial saliva can increase the degree leading to an alkaline pH, while P. gingivalis and L. acidophilus can be reduced of colonies of C. albicans hypha and able to inhibit the growth of C. albicans. Nevertheless, both bacteria can be bacteriostatical against C. albicans. Keywords: Candida albicans, Porphyromonas  gingivalis, Lactobacillus acidophilus, saliva pH and Oral candidiasis
POTENSI EKSTRAK ALGA MERAH Gracilaria verrucosa SEBAGAI PENGHAMBAT PERKEMBANGAN PEMBENTUKAN BIOFILM Enterococcus faecalis PADA INFEKSI SALURAN AKAR GIGI Putri Rahmi Noviyandri; Ridha Andayani; Ervina Rizky
Journal of Syiah Kuala Dentistry Society Vol 3, No 1 (2018): JANUARY
Publisher : Dentistry Faculty

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Abstract

Enterococcus faecalis (E. faecalis) is a facultative anaerobic Gram-positive bacteria which becomesthe main bacteria occurrence failure of endodontic treatment. The red algae Gracilaria verrucosa (G. verrucosa) is one of the herbal plants that contain antibacterial compounds such as alkaloid,flavonoid, saponin, steroid, and triterpenoid. This study aims to determine the potency red algae G.verrucosa extract as inhibitor of E. faecalis biofilm formation development. The red algae G.verrucosa extract with a concentration of 100%, 75%, 25%, 12,5%, and 6,25% was made inmaceration method using ethanol 96%. Microtiter Plate Biofilm Assay method and ELISA readerwith wavelength 595nm were used to test the biofilm. The result of One Way ANOVA showedp0,05 which mean there was an effect of the test group on the formation of E. faecalis biofilm development and Post Hoc Tests-Duncan showed that there was a significant differences betweeneach group of those concentration. In the treatment group, there was found two concentration thathave significant differences, there were 100% and 6,25%. Based on the result of this study, we canconclude that the red algae G. verrucosa extract was useful as an inhibitor of E. faecalis biofilmformation development. The higher concentration of the red algae G. verrucosa, the higher its abilityas an inhibitor of E. faecalis biofilm formation development.Keywords : Enterococcus faecalis, red algae Gracilaria verrucosa, Microtiter Plate Biofilm Assaymethod, ELISA reader.
HUBUNGAN STRES NEGATIF (DISTRES) DENGAN PERDARAHAN GINGIVA PADA PASIEN RUMAH SAKIT JIWA BANDA ACEH Sunnati .; Ridha Andayani; C Putroe Ula Rahifa; Sri Rezeki
Journal of Syiah Kuala Dentistry Society Vol 4, No 1 (2019): OKTOBER
Publisher : Dentistry Faculty

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Abstract

Gingival bleeding is an early feature of gingivitis. Gingivitis is characterized by gingival redness,swelling, and bleeding on probing. Gingival bleeding may be caused by local factor and systemicfactors. Negative stress (distress) is thought to be the risk factor of gingival bleeding. Previous studiesshowed that negative stress (distress) may contribute to the severity of gingival bleeding. However, insome cases heavy gingival bleeding was found in the presence of minimal plaque retention. Theobjective of this study was to see the correlation between negative stress (distress) and gingivalbleeding. This study was an analytic cross-sectional study taking Banda Aceh mental hospital. Thesubjects of this study were 58 patients aged between 15 – 60 years old. There were 30 male and 28female patients. Data was collected from medical record, history taking and clinical examination ofgingival bleeding and oral hygiene index-simplified (OHI-S). Statistic analysis using chi-square testrevealed that there was no correlation between negative stress (distress) to gingival bleeding (p0.05).Conclusion: This study shows that there is no significant correlation between negative stress (distress)to gingival bleeding. Keywords: Gingival bleeding, negative stress (distress), OHI-S
AKTIVITAS ANTIBAKTERI TEPUNG CACING TANAH (Lumbricus rubellus) TERHADAP Enterococcus faecalis SECARA IN VITRO Ridha Andayani; Zaki Mubarak; Dian Rizki Rinanda
Journal of Syiah Kuala Dentistry Society Vol 1, No 2 (2016): JULY
Publisher : Dentistry Faculty

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Abstract

AbstractEnterococcus faecalis is a facultative anerobic bacterium which can causesecondary periapicalinfection and is very resistant to numerous antimicrobial substances normally used during the rootcanal treatment. Earthworm (Lumbricusrubellus) possess antimicrobial peptide, known as Lumbricin-1 which is known tohinder the growth of Gram positive and Gram negative bacteria as well as fungi,but rarely caused resistance. This research was an experimental laboratory study conducted to observethe antibacterial activity of Lumbricin-1 contained in earthworm powder towards the growth of E.faecalis in vitro. Enterococcusfaecalis was cultured on CHROMagar VRE media and incubatedanaerobically for24-48 hours in the temperature of 37⁰C. The bacterium was identified by observingthe colour of the colony of the bacterium growing on the CHROMagarVRE medium and Gramstaining, while antibacterial activity test was performedusing disk diffusion method. Statisticalanalysis using one way ANOVA and Duncan test showed that there was a significant difference (p 0,05) between test and control group. The result of the study showed that earthworm powderpossessed strong antibacterial activity towards the growth of Enterococcusfaecalis.Key words: Enterococcus faecalis, Lumbricus rubellus, antimicrobial peptide, Lumbricin-1