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Eryk Andreas
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Agriculture, Biological Sciences & Forestry

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Keragaman Gen K-Kasein dan Hubungannya dengan Produksi dan Kualitas Susu pada Domba di Unit Pendidikan dan Penelitian Peternakan (UP3) Jonggol Cece Sumantri; Eryk Andreas; Achmad Farajallah; Jarmuji Jarmuji
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 1 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objectives of this study were to identify polymorphisms in ovine K-casein gene exon 4 and its association with milk yield, percentage of protein and milk fat in sheep under extcnsif management at JASTRU (Jonggol Animal Studies Teaching and Research Unit) farm, Fact. of Anim Sci, Bogor Agric. University. The 83 blood sample from ewes which have record of milk production were used to determined polymorphism by using PCR-SSCP (Polymerase Chain Reaction-Single Strand Conformation Polymorphism) methode. The research activities were carried out through blood collection, DNA isolation, and fragment DNA amplification by PCR and separation in electrophoresis with silver staining. Electrophoretic pattern (PCR-SSCP) revealed th rce types of K-casein gene were dcsignitcd A, Band C type. The highest proportion was A type 69.57% and followed hy B 28.99% and the lowest C 1.45%. The were no significant effect of types of of K-casein gene on milk production and milk quality (percentage of protein and fat) in local sheep from Jonggol.   Keywords: .Jonggol sheep, K-cascin gcn, PCR-SSCP, polymorphism
KERAGAMAN GEN CALPASTATIN, CALPAIN 3 DAN MYOSTATIN PADA DOMBA DI UP3 JONGGOL Cece Sumantri; . Jakaria; Mohamad Yamin; Henny Nuraini; Bramada Winiar Putra; Eryk Andreas
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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The aim of this study was to identify the genetic polymorphisms of calpastatin (CAST), calpain 3 (CAPN3) and myostatin (MSTN) on local sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU). A total number of 294 blood samples were collected from JASTRU. The identification of polymorhism in CAST and CAPN3 genes performed by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) while MSTN gene by using PCR-SSCP methods. The results showed that CAST|MspI, CAST|NcoI and CAPN3|MaeII loci were polymorphic, whereas The MSTN locus was monomorphic for G (1.0). The frequency of allele M (0.87) on the locus (CAST|MspI) higher than the N allele (0.13). At locus CAST|NcoI, the frequency of allele M (0.96) higher than the N allele (0.04). At the CAPN3|MaeII, allele G (0.85) and allele T (0.15). Locus CAST|NcoI has higher observed heterozygosity (Ho = 0.92) compared to CAPN3|MaeII and CAST|MspI (Ho = 0.74-0.77), however has lower compared to CAPN3|MaeII and CAST|MspI in expected of heterozygosity (He = 0.08 vs 0.23-0.26) and in index fixation (Fis = -0.04 vs 0.03-0.12).
Co-Authors ACHMAD FARAJALLAH Bramada Winiar Putra Cece Sumantri Henny Nuraini Jakaria Jakaria Jarmuji Jarmuji Mohamad Yamin