Claim Missing Document
Check
Articles

Found 6 Documents
Search

Perbedaan Ekspresi Caspase 3 pada Limpa Tikus Model Sepsis yang Diinfeksi Escherichia coli ESBL dan dengan yang Diinfeksi Klebsiella pneumoniae Carbapenemase Lisa Savitri; Willy Sandhika; Agung Dwi Wahyu Widodo
Prosiding Seminar Nasional Multidisiplin Vol 2 No 1 (2019): Volume 2 No 1 Tahun 2019 (On Proses)
Publisher : KH. A. Wahab Hasbullah University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sepsis adalah suatu sindroma klinik yang terjadi karena adanya respon tubuh yang berlebihan terhadap rangsangan produk mikroorganisme. Pasien sepsis dengan infeksi bakteri penghasil ESBL 57,4% terinfeksi E. coli, 21,35% terinfeksi Enterobacter sp, dan 21,3% terinfeksi Klebsiella sp. Sepsis dapat diperberat oleh peningkatan kuman yang multiresisten terhadap bermacam antibiotik, seperti E. coli ESBL dan K. pneumoniae carbapenemase (KPC). Selama infeksi bakteri, faktor virulen diproduksi dan disekresikan dari patogen dan memicu sinyal apoptosis. Penelitian mengenai caspase 3 dianggap penting, karena caspase 3 merupakan efektor caspase terpenting yang bertanggungjawab atas morfologi dan perubahan biologis yang terlihat pada sel apoptosis. Jenis penelitian ini adalah true experimental dengan rancangan penelitian post test only control group design. Hasil penelitian menunjukkan bahwa ekspresi caspase 3 pada limpa mencit yang diinfeksi KPC adalah 65,25±12,69%, sedangkan yang diinfeksi E. coli ESBL adalah 33,75±3,862%, hal tersebut diduga karena adanya kapsul polisakarida yang mengelilingi KPC dan melindungi dirinya terhadap aksi fagositosis dan bakterisidal serum yang dapat dianggap sebagai penentu virulensi paling penting dari KPC dan menyebabkan mitokondria melepaskan ROS. Infeksi bakteri tersebut menyebabkan mitokondria memproduksi ROS dan memicu pelepasan sitokrom c. Sitokrom c akan memicu caspase 9 untuk berikatan dengan efektor caspase 3, sehingga terjadi apoptosis Kata kunci: Sepsis, ekspresi caspase 3, limpa tikus, Escherichia coli ESBL, Klebsiella pneumoniae carbapenemase
Animal Models with Metabolic Syndrome Markers Induced by High Fat Diet and Fructose Larantika Hidayati; Agung Dwi Wahyu Widodo; Boerhan Hidayat
Medical Laboratory Technology Journal Vol 6, No 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v6i1.266

Abstract

Metabolic syndrome is lipid and non-lipid metabolism disorder due to the association of several factors such as physiological, clinical, biochemical, and interrelated factors. People with metabolic syndrome can be diagnosed by fulfilling 3 of 5 criteria, including obesity and increased waist circumference, increased TG levels, increased blood pressure, hyperglycemia, and increased High-Density Lipoprotein (HDL) serum. The high-fat diet disrupts tissue lipid metabolism, so insulin resistance occurs due to lipotoxicity. Besides, some studies use a combination of mixtures (fructose, sucrose) and fat-rich food components to build metabolic characteristics in mice that affect human characteristics. The purpose of this study was to make an animal model with a metabolic syndrome marker induced by the High Fat Diet (HFD) consisting of pork oil and chicken egg yolk, as well as fructose from simple and economical ingredients. This study was an experimental study using experimental animals of male Rattus norvegicus strain wistar, which were grouped into three random treatment groups, namely the control group, HFD group, and High Fat Diet Fructose (HFDF) group. The number of samples used was 27 rats, with nine rats in each group. The animal was induced for four weeks, then measured levels of FPG, HD, and TG. From the research that has been done, it is found that there are significant differences in levels of Fasting Plasma Glucose (FPG), High-Density Lipoprotein (HDL), and Triglycerides (TG) (p
PROFIL RASIO NEUTROFIL TERHADAP LIMFOSIT PADA PASIEN DENGAN DUGAAN DEMAM TIFOID DI RSUD DR. SOETOMO SURABAYA Meiwinda Rizky Nurhidayah; Muhammad Vitanata Arfijanto; Agung Dwi Wahyu Widodo; Ulfa Kholili
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 9, No 1 (2021)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jc.v9i1.2042

Abstract

Demam tifoid merupakan penyakit multisistem akut yang disebabkan oleh Salmonella typhi dan bersifat endemis. Rendahnya sensitivitas alat diagnostik menyebabkan under diagnosed maupun over diagnosed. Beberapa studi menggunakan rasio neutrofil limfosit sebagai parameter status inflamasi. Studi ini untuk mengetahui gambaran rasio neutrofil limfosit pada pasien dengan dugaan demam tifoid. Sebuah studi deskriptif observasional dengan desain lintang potong menggunakan data sekunder dari rekam medis di Rumah Sakit Umum Daerah Dr. Soetomo Surabaya tahun 2016-2018. Teknik pengambilan sampel adalah total sampling dan diperoleh 64 sampel. Jumlah pasien dengan jenis kelamin laki-laki sebanyak 53,1% dan perempuan sebanyak 46,9% Perbandingan pasien laki-laki dan perempuan 1,3:1 dengan rentang usia terbanyak 17-25 tahun. Gambaran klinis rerata lama demam yaitu 6,95 hari dengan rentang lama demam terbanyak 1-7 hari. Demam ditunjukkan pada semua pasien (100%). Pemeriksaan hematologi kadar hemoglobin, leukosit dan trombosit dalam batas normal. Hasil neutrofil dan limfosit dalam batas normal. Gambaran rasio neutrofil limfosit memiliki median 3,97 dengan rentang nilai dalam batas normal.
Effects of Prebiotics, Probiotics, and Synbiotics on the Bodyweight, Blood Glucose, Triglyceride and TNF-α of Diet-induced Obesity Rats Lenny Octavia; Soebagijo Adi Soelistijo; Agung Dwi Wahyu Widodo
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 4, No 2 (2020)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v4i2.4206

Abstract

Abstract  High-fat diet leads to obesity-associated chronic low-grade inflammation. Prebiotics, probiotics, and synbiotics produced short-chain fatty acids (SCFA), bonded to G protein-coupled receptors (GPR)-41 and GPR-43 decreased triglyceride deposits in adipocytes and liver, decreased fatty acid oxidation, increased glucose regulation and insulin sensitivity thus reduced the risk of obesity and metabolic syndrome. This study conducted in order to evaluate the effects of prebiotics, probiotics, and synbiotics on the body weight, blood glucose, triglyceride, and TNF-α used rats model, which were fed by a high-fat diet. Thirty-eight 6-8 weeks old male rats were fed by high-fat diet for three weeks, then rats were randomly divided into four groups, high-fat diet (HFD), a high fat diet with prebiotics supplementation (HFD+ PRE), a high fat diet with probiotics supplementation (HFD+PRO), and high-fat diet with synbiotics supplementation (HFD+SYN) for three weeks. Blood samples and body weight were measured at the third and sixth week. There was no effect of prebiotics, probiotics, and synbiotics on body weight, triglyceride levels, blood glucose, and TNF-α in rats fed a high-fat diet compared to control. These results suggested that supplementations gave inconsistent results with other studies and needed further researches.Keywords             : high fat diet, prebiotics, probiotics, synbiotics, meta-inflammationCorrespondence   : soebagijo@yahoo.com
In Vitro Antibacterial Activity of Waste Palm Cooking Oil Against Staphylococcus Aureus Fiqih Faizara Ustadi; Agung Dwi Wahyu Widodo; Yuani Setiawati
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17499

Abstract

Background: Staphylococcus aureus is a Gram-positive coccus which acts as a pathogen causing awide range of infectious diseases. In recent years, several strains of S. aureus have been found to showresistance to several antibiotics. Waste cooking oil may be considered as an alternative antibacterialproduct, as it contains long-chain fatty acids whose antibacterial effectiveness against S. aureus hasbeen known for years. In addition, oxidative biocides produced during the frying process have manytargets for antibacterial activity in the cell and affect almost every biomolecule. Nonetheless, there isno literature that is able to prove the antimicrobial effects of the waste palm cooking oil. Objective: Toexamine the in vitro antibacterial effect of waste palm cooking oil against S. aureus. Method: a twofoldserial dilution method to set the minimum level of both inhibitory and bactericidal concentrations.Conclusion: This study showed that waste palm cooking oil did not show antibacterial effects againstS. aureus, indicating that waste palm cooking oil is not possibly to be applied as an antibacterial agentagainst S. aureus.
Analysis of Staphylococcus aureus and Escherichia Coli and its Susceptibility to Antibiotic in Catheter-Associated Urinary Tract Infection Patients at Hospital in Province of West Nusa Tenggara BaiqIsti Hijriani; ManikRetno Wahyunitisari; Agung Dwi Wahyu Widodo
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 2 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i2.17945

Abstract

Objective – This study aimed to analyze the proportion of Staphylococcus aureus and Escherichia colifrom urine samples of catheter-associated urinary tract infections (CA-UTI) patients at the GeneralHospital at Province of West Nusa Tenggara. Also measured the pattern of sensitivity to severalantibiotics.Methods –This study is a descriptive observational with a cross-sectional approach. A total of 60 sampleswere used in this study. Bacterial identification was carried out according to standard bacteriologicalculture techniques. Furthermore, the antibiotic sensitivity test following the Kirby-Bauer disc diffusionmethod using several antibiotics, including amoxycillin (AMP), ciprofloxacin (CIP), ceftriaxone(CRO), and sulphamethoxazole trimethoprim (SXT).Results –The results showed that bacteria causing CA-UTI were Staphylococcus aureus (43.33%),Escherichia coli (21.67%), Staphylococcus epidermidis (10%), Proteus mirabilis (6.67%), Enterobacteraerogenes (6.67%), Serratica marcescens (5%), Klebsiella sp (3.33%), and Pseudomonas sp (3.33%).The antibiotic susceptibility test found that 4.58%, 11.25%, 9.16%, and 3% of bacteria were resistant toamoxycillin, ceftriaxone, ciprofloxacin, and sulphamethoxazole trimethoprim, respectively.Conclusion –Staphylococcus aureus and Escherichia coli were the most gram-positive and gramnegativebacteria causing CA-UTI, respectively. The most resistant antibiotic was ciprofloxacin, andthe most sensitive antibiotic is sulphamethoxazole trimethoprim.