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Reorganization of Breeding Institutions to Develop Breeding Stock of Indonesian Holstein Talib, C; Anggraeni, A; Diwyanto, K
Indonesian Bulletin of Animal and Veterinary Sciences Vol 11, No 2 (2001)
Publisher : Indonesian Animal Sciences Society

Development of dairy cattle production through breeding system improvement is not well arranged for guaranteeing a successful to increase capability of Indonesian milk industries. Some constraints that inhibit the programs are only a short-term program available to improve cattle production ie., improvement of rearing and feeding management; a small number of finance available; no appropriate joint-action between breeding institutions; and the operational of recording program and progeny test are very limited. Other factors that also directly or indirectly influence the development of dairy cattle production are only a small number of animal unite is kept by farmer, limited land area per family, no access for banking supported, and relatively small upland area available in comparison to milk requirement in Indonesia. Improvement of breeding program by way of artificial insemination (AI) is good conducted but the problem is the bulls for producing AIâs semen are not tested under Indonesian condition. Therefore, proven bulls that tested in Indonesia has to be priority together with identification for highly producing cows. All activities can be well organized if the recording program recorded by farmer or some one else and all breeding institutions can rearrange their programs to produce a better mechanism for supporting Indonesian milk industries. Â Key words: Institution, breeding, Indonesian Holstein

Variation on DNA microsatellite of Holstein-Friesian dairy cows in Baturaden Dairy Cattle Breeding Center Sumantri, C; Anggraeni, A; Farajallah, A; Perwitasari, D
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Eight microsatellite loci of which the locations were three on chromosome 6 (BTA6) namely CSN 3, BM 143 and BM 415; two on chromosome 9 (BTA9) namely ETH 225 and BM 4208; and three on chromosome 10 (BTA10) namely BP 31, BM 1237 and BM 888 were used to investigate genotypic variation of Holstein-Friesian (HF) dairy cows in Baturraden Dairy Cattle Breeding Centre. Research activities were carried out through some steps involving blood collection, DNA isolation, amplification on DNA fragments by polymerase chain reaction (PCR) and separation by electrophoresis with silver staining. Frequency and heterozygosity of genes under consideration were calculated. The results showed that the eight microsatellite loci exhibited a total number of 33 alleles. Locations of those alleles were five in BM 143 and BM 4208 loci; four in BM 415, CSN 3, ETH 225, BM 1237, BM 888 loci; and three in BP 31 locus. A number of 14 out of 33 alleles might be specific alleles for HF dairy cattle in Baturraden Dairy Cattle Breeding Centre. The lowest heterozygosity per locus (Ä¥) was 0.6151 for BM 415 whilst the highest one was 0.7301 for BM 888. Additionally, the average heterozygosity for all loci (Ä¤) detected in this study was 0.6768. The genotype BB on locus BM 143 and AC on locus CSN-3 significantly affected (P<0.05) the estimated breeding value of milk yield of HF cows in this location. Key Words: Holstein-Friesian Cows, DNA Microsatellite, Allele, EBV of Milk Yield

Aplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Province Sumantri, Cece; Diyono, R; Farajallah, A; Anggraeni, A; Andreas, E
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

Selection using genetic markers are commonly performed to improve livestock productivity in the livestock industry. The objectives of this study were to identify growth hormone genes family (GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI) polymorphisms of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. A total number of 209 blood samples were collected from 15 districts. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products of GH, GHR, GHRH and Pit-1 Genes were digested with MspI, AluI, HaeIII and HinfI enzyme restriction. Fragments of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI were detected by EtBr method. The results showed that GH|MspI and GHRH|HaeIII loci were polymorphic, GH|AluI, GHR|AluI and Pit-1|HinfI, loci were monomorphic. GH allele (-) at locus GH|MspI was only found in Cisata (0.03) and Menes (0.11). Allele B at locus GHRH|HaeIII only found in Cibadak (0.42), Cisata (0.30) and Menes (0.11). In the total population of Banten locus GH|MspI have low diversity (He = 0.02) and polymorphic information content (Pic = 0.02), whereas GHRH|HaeIII locus has a higher diversity (He = 0.23) and Pic (0.22). Key Words: Polymorphism, Growth Hormone Genes, Buffalo

Association between Polymorphism of Î²-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Sumantri, Cece; D, Nurhayati; Farajallah, Ahmad; Anggraeni, A
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The aims of this study were to identify the effects polymorphism of Î²-lactoglobulin geneÂ on milk yield and percentage of milk proteinÂ andÂ fatÂ inÂ local sheep. A total number of 83Â heads ofÂ lactating ewesÂ raised under an extensive management atÂ Jonggol Animal Science Teaching and Research Unit (JASTRU) ofÂ the Faculty of Anima Science, Bogor AgriculturalÂ University wereÂ studied.Â ResearchÂ activitiesÂ wereÂ carriedÂ outÂ throughÂ someÂ step involving blood collection. DNA isolation, DNA amplification and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (PÂ R) then genotyped by single strandÂ conformation polymorphism technique (SSCP), it was success fully amplified a fragment length at 420 bp in Î²-lactoglobulin gene located onÂ the exon 7. The electrophoresis Â patternÂ revealed 5 types of Î²-lactoglobulin gene and designated as AB. C, D and E types. The proportion forÂ those respective 5 type from theÂ highest toÂ the lowest were for A (27.7%)C (16.87%), D (12.05%), (10.84%) and B (9.64%)Â respectively. There were noÂ significant effectÂ polymorphism Î²-lactoglobulin gene on both milk yield and qualityÂ inÂ IÂ calÂ sheep at JASTRU. Â Keywords : polymorphism. Î²-lactoglobulin in gene, PCR-SSCP and local sheep

Hubungan Masa Kosong dengan Produktivitas pada Sapi Perah Friesian Holstein di Baturraden, Indonesia A Atabany; B P Purwanto; T Toharmat; A Anggraeni
Media Peternakan Vol. 34 No. 2 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Productivity of dairy cows not only expresed by milk yield, but also the reproductive performance. General speaking that the milk yield depends on reproductive performance and vice versa. The present study was conducted to observe the milk yield and reproductive performance of Friesian Holstein (FH) dairy cows. This study was conducted using 150 sets of weekly recording of milk yield from 50 FH cows. Milk yield was calculated by using time interval method (TIM) which was equivalent to ME 305 days. The data were analysed using polynomial regression model. Results of the study indicated that the effect of days open on milk yield had significant effect on quadratic and qubic regression model. The average days open was 138.77 days. According to the quadratic regression model, increasing days open decreased milk yield and the minimum milk yield was observed at 169 days open.

Growth Hormone Gene Polymorphism and Its Association with Partial Cumulative Milk Yields of Holstein Friesian Dairy Cattle R Misrianti; A Anggraeni; E Andreas; C Sumantri
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Growth hormone gene (GH gene) plays an important role in regulating body growth and in developing mammary gland, similar with its interaction to specific receptors. The GH gene has been considered as one of candidate gene associated with selection on lactation trait and milk production. This study was aimed to determine genetic polymorphism of the GH-AluI gene and to associate its genotype variants on various 15-d partial cumulative milk yields in Holstein Friesian (HF) dairy cows. A number of 370 blood samples were collected from six HF populations, respectively from small dairy farmer under the supervision of the North Bandung Milk Cooperation (NBMC) in Cilumber (98) and Pasir Kemis village (96), Dairy Cattle Breeding and Improvement Station (Cikole DCBIS) Cikole (88), Lembang Artificial Insemination Center (Lembang AIC) (17), Singosari Artificial Insemination Center (Singosari AIC (32), and Cipelang Livestock Embryo Center (Cipelang LEC) (40). A polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method was used to identify variant genotypes of the GH gene using AluI restriction enzyme. Genotyping results produced only two genotypes, i.e. LL and LV genotypes, without VV genotype. Frequency of the former was dominant, whilst that was low for the latter (89% vs. 11%); leading to the frequency of L allele was very high (94%) compared to that of V allele (6%). No significant association between variant genotypes (LL and LV) and various 15-d partial cumulative milk yields.

Aplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Province Cece Sumantri; R Diyono; A Farajallah; A Anggraeni; E Andreas
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 4 (2010): DECEMBER 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Selection using genetic markers are commonly performed to improve livestock productivity in the livestock industry. The objectives of this study were to identify growth hormone genes family (GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI) polymorphisms of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. A total number of 209 blood samples were collected from 15 districts. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products of GH, GHR, GHRH and Pit-1 Genes were digested with MspI, AluI, HaeIII and HinfI enzyme restriction. Fragments of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI were detected by EtBr method. The results showed that GH|MspI and GHRH|HaeIII loci were polymorphic, GH|AluI, GHR|AluI and Pit-1|HinfI, loci were monomorphic. GH allele (-) at locus GH|MspI was only found in Cisata (0.03) and Menes (0.11). Allele B at locus GHRH|HaeIII only found in Cibadak (0.42), Cisata (0.30) and Menes (0.11). In the total population of Banten locus GH|MspI have low diversity (He = 0.02) and polymorphic information content (Pic = 0.02), whereas GHRH|HaeIII locus has a higher diversity (He = 0.23) and Pic (0.22). Key Words: Polymorphism, Growth Hormone Genes, Buffalo

Variation on DNA microsatellite of Holstein-Friesian dairy cows in Baturaden Dairy Cattle Breeding Center C Sumantri; A Anggraeni; A Farajallah; D Perwitasari
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Eight microsatellite loci of which the locations were three on chromosome 6 (BTA6) namely CSN 3, BM 143 and BM 415; two on chromosome 9 (BTA9) namely ETH 225 and BM 4208; and three on chromosome 10 (BTA10) namely BP 31, BM 1237 and BM 888 were used to investigate genotypic variation of Holstein-Friesian (HF) dairy cows in Baturraden Dairy Cattle Breeding Centre. Research activities were carried out through some steps involving blood collection, DNA isolation, amplification on DNA fragments by polymerase chain reaction (PCR) and separation by electrophoresis with silver staining. Frequency and heterozygosity of genes under consideration were calculated. The results showed that the eight microsatellite loci exhibited a total number of 33 alleles. Locations of those alleles were five in BM 143 and BM 4208 loci; four in BM 415, CSN 3, ETH 225, BM 1237, BM 888 loci; and three in BP 31 locus. A number of 14 out of 33 alleles might be specific alleles for HF dairy cattle in Baturraden Dairy Cattle Breeding Centre. The lowest heterozygosity per locus (ĥ) was 0.6151 for BM 415 whilst the highest one was 0.7301 for BM 888. Additionally, the average heterozygosity for all loci (Ĥ) detected in this study was 0.6768. The genotype BB on locus BM 143 and AC on locus CSN-3 significantly affected (P<0.05) the estimated breeding value of milk yield of HF cows in this location. Key Words: Holstein-Friesian Cows, DNA Microsatellite, Allele, EBV of Milk Yield

Reorganization of Breeding Institutions to Develop Breeding Stock of Indonesian Holstein C Talib; A Anggraeni; K Diwyanto
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 11, No 2 (2001)
Publisher : Indonesian Center for Animal Research and Development

Development of dairy cattle production through breeding system improvement is not well arranged for guaranteeing a successful to increase capability of Indonesian milk industries. Some constraints that inhibit the programs are only a short-term program available to improve cattle production ie., improvement of rearing and feeding management; a small number of finance available; no appropriate joint-action between breeding institutions; and the operational of recording program and progeny test are very limited. Other factors that also directly or indirectly influence the development of dairy cattle production are only a small number of animal unite is kept by farmer, limited land area per family, no access for banking supported, and relatively small upland area available in comparison to milk requirement in Indonesia. Improvement of breeding program by way of artificial insemination (AI) is good conducted but the problem is the bulls for producing AI’s semen are not tested under Indonesian condition. Therefore, proven bulls that tested in Indonesia has to be priority together with identification for highly producing cows. All activities can be well organized if the recording program recorded by farmer or some one else and all breeding institutions can rearrange their programs to produce a better mechanism for supporting Indonesian milk industries. Key words: Institution, breeding, Indonesian Holstein

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