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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Sains dan Seni ITS Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science Jurnal Sains Dan Teknologi (SAINTEKBU) Inscientech: Journal of Industrial, Science and Technology Jurnal Agronomi Tanaman Tropika (JUATIKA) Jurnal HPT (Hama Penyakit Tumbuhan)
Anton Muhibuddin
Jurusan Hama Dan Penyakit Tanaman, Fakultas Pertanian, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Arts Humanities Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Environmental Science Industrial & Manufacturing Engineering Library & Information Science Mechanical Engineering

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SOIL FERTILITY STATUS AND SOYBEAN [Glycine max (L) Merr] PERFORMANCE FOLLOWING INTRODUCTION OF INDIGENOUS MYCORRHIZA COMBINED WITH VARIOUS NUTRIENT SOURCES INTO SANDY SOIL Astiko, Wahyu; Sastrahidayat, Ika Rocdjatun; Djauhari, Syamsuddin; Muhibuddin, Anton
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The experiment tested four treatments, namely inoculation of soil with mycorrhiza, inoculation of soil with mycorrhiza and cattle manure, inoculation of soil with mycorrhiza and rock phosphate, and inoculation of soil with mycorrhiza and inorganic fertilizers. Soil without any inoculation was provided as control. The treatments were arranged in a Completely Randomized Design with four replications. Soil fertility status was based on the concentrations of N, P, K, and organic-C as well as soil pH. Plant performance was determined based on its ability to uptake nutrients (N, P, K, and Ca), its growth and yield. Then, the activity of mycorrhiza was measured based on total population and percentage of root infection. Overall, results of the present study showed that, compared with other treatments, inoculation of mycorrhiza into soil and amended with cattle manure significantly performed higher concentrations of N, P, K, and organic-C. This soil condition caused soybean to absorb significantly higher nutrients, grew well with higher yield compared with plant performance in other treatments. Therefore, results of this study implies that the application of mycorrhiza into soil amended with organic matter is promising to sustain soil productivity under soybean cropping system.  Keywords: Arbuscular Mycorrhiza, soil fertility, soybean yield, dryland
Control of “Damping Off” Disease Caused by Sclerotium Rolfsii Sacc. using Actinomycetes and Vam Fungi on Soybean in The Dry Land Based on Microorganism Diversity of Rhizosphere Zone Rochjatun, Ika; Djauhari, Syamsuddin; Saleh, Nasir; Muhibuddin, Anton
AGRIVITA, Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

One of the obstacles in the efforts to increase soybean production in Indonesia is disease such as damping off which is caused by Sclerotium rolfsii. In East Java, the intensity of S. Rolfsii reached approximately 8.61% that spread all over Indonesia region, even in our neighbor countries such as Malaysia, Thailand and the Philippines. This research was carried out to determine the efficacy of Actinomycetes and VAM (Vesicular Arbuscular Mycorrhizal) against damping-off attack and the diversity of microorganisms in rhizosfer. Research conducted in the laboratory and screen house on Plant Protection Department, Faculty of Agriculture, University of Brawijaya and in Lawang District Malang. Observation variables include level of pathogen attack and infection rate of dampingoff pathogen. Plant height, number of pods, pod weight, seed weight and weight of 100 seeds from each treatment, diversity and identification of microorganisms in rhizosphere were also observed. The results showed that Actinomycetes and VAM application could decrease the percentage of plant death due to damping-off.  Application of Actinomycetes and VAM gave effect on microorganism diversity of Ratai Rhizosphere but not on Wilis. Keywords: Sclerotium rolfsii, vesicular arbuscular mycorrhizal, actinomycetes, and rhizosphere
Biodiversity of Soil Fungi on Integrated Pest Management Farming System Muhibuddin, Anton; Addina, LuÂ’aili; Abadi, Abdul Latief; Ahmad, AthoÂ’illah
AGRIVITA, Journal of Agricultural Science Vol 33, No 2 (2011)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

The greatest problem of modern agricultural practices is the use of chemical pesticides and fertilizers. It is noted that about 40% of the world’s land surface is used for agriculture. The way this agricultural land is managed has a great influence on the global environment. Integrated Pest Management (IPM) farming system produces fewer negative externally affects, can effectively restore ecosystems and deliver well ecosystem services. Depending on methods applied and degree of production, agricultural practices impact biodiversity in the ecosystem and it also influences conservation practices within the ecosystem. The result showed that diversity of soil fungi on IPM was higher than diversity on conventional one. Trichoderma sp. and Acremonium sp. which are known as antagonist fungi were found on IPM but not on conventional one. Domination Index on IPM was lower than conventional field. Bacterial leaf blight (Xanthomonas oryzae) disease intensity showed no difference on both systems. The yield on IPM was higher (6.34 ton/ha) than conventional field (5.56 ton/ha). It has been found that rich biodiversity in agricultural environment improves productivity of agricultural systems. The IPM system gives more regard to the soil fungi biodiversity and considers it as an integrated system upon which the success of the agricultural production depends.Keywords : Integrated Pest Management, biodiversity, soil fungi, modern agricultural
ARBUSCULAR MYCORRHIZA FUNGI AS AN INDICATOR OF SOIL FERTILITY Muhibuddin, Anton; Djauhari, Syamsuddin; Syibli, Muhammad Akhid
AGRIVITA, Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Arbuscular mycorrhizal fungi (AMF) are ubiquitous organism that forms association with the root of most terrestrial plants. AMF association also influence soil fertility through the enhancement of chemical, biological and physical content. In this study, we enumerated AMF spores from rhizosphere of Tithonia difersivolia as an indicator of soil fertility. The results showed that the most fertile soil had the highest AMF spores density. This research has confirmed that AMF has high interaction with organic carbon, organic matter, total phosphorus, cation exchange capacity, water level, soil fungi and soil bacteria. Partial regression analysis revealed the mathematic equation for their interaction. This equation used the abundant of AMF spores as an indicator for chemical, biological and physical fertility of the soil.                 Keywords: Arbuscular mycorrhiza fungi (AMF), indicator, soil fertility, Tithonia difersivolia  
Yeast On Epiphyte of Tangerines Citrus Fruit (Citrus nobilis L.) and Their Potential Antagonist to Colletotrichum Gloeosporioides (Penz.) Penz. & Sacc. Pramudita, Oki; Muhibuddin, Anton
Inscientech: Journal of Industrial, Science and Technology Vol 1 No 02 (2017): Inscientech: Journal of Industrial, Science and Technology
Publisher : Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/inscientech.v1i02.3

Abstract

One of the most important diseases of citrus plant is anthracnose which caused by Colletotrichum gloeosporioides (Penz.) fungi. Yeast is one of microbes which has a good potential to control the disease. This research focused on exploring yeasts on tangerines and examining their efficacy to control C. gloeosporioides using in vitro and in vivo treatments. Observation was started by isolated C. gloeosporioides and yeast from tangerines citrus fruit, then followed with in vitro and in vitro treatment. Both were counted the percentage of antagonist and pathogen incubation stage and also disease incidence, respectively. Result showed that yeast has been isolated from tangerines fruit were Candida sp. (isolate 1), Candida sp. (isolate 2) and Pichia sp. All yeast showed inhibit C. gloeosporioides growth and suppressed its development.
Correlation between Soil Phospor (P) Content and Soil Yeast Antagonistics Ability Against Fusarium oxysporum Pathogen Causing Wilt Muhibuddin, Anton; Wibowo, Kartika Novitasari; Punnapayak, Hunsa; Goetz, Peter
Inscientech: Journal of Industrial, Science and Technology Vol 1 No 02 (2017): Inscientech: Journal of Industrial, Science and Technology
Publisher : Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/inscientech.v1i02.1

Abstract

Besides its role during fermentation process, yeast also has potential as biological agent because of its antagonistics characteristic. We have isolated yeast from six different locations based on Phospourus differences. Isolated yeast were then tested its antagonistics ability of Fusarium oxysporum, the pathogen causing tomato wilt disease. This research aims to find out the diversity of yeast found in the tomato rhizosphere in six different contents of phosphorus locations and to know its antagonistics ability against the pathogenic. F. oxysporum. This research was conducted in the Laboratory of Mycology, Department of Pests and Diseases, Faculty of Agriculture, Brawijaya University, Malang and in the Chemistry Laboratory, Institut Teknologi 10 Nopember Surabaya, started from January up to September 2015. Yeasts have been isolated from tomato’s rhizosphere of 6 different locations around East Java proviences. The result showed that yeasts from organic field (Lower P content) were 6 genera. They are Candida sp. 1, Pichia sp. 1, Hansenula sp., Metschnikowia sp. 1, Cryptococcus sp., and Zygosaccharomyces sp. While the yeasts from inorganic field (higher P) were 3 genera. The most potential yeast in controlling F. oxysporum is Pichia sp. 2. Lower P content showed more divers than higher P content. Yeasts from higher soil P content showed more antagonists to control F. oxysporum. Keywords: Phosphor, correlation, yeast, antagonistic, disease, soil
Potential of Wild Yeast from Banana to Control Colletotrichum musae Fungi Caused Anthracnose Disease and Its Short Antagonistic Mechanism Assay Muhibuddin, Anton; Sektiono, Antok Wahyu; Sholihah, Dewi Maratus
Journal of Tropical Life Science Vol 9, No 1 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Anthracnose caused by Colletotrichum musae is one of several important disease in banana fruit. An effective, cheap, and safe control method are necessary as a postharvest disease control alternative. This research aimed to obtain and identify wild yeast from banana which was isolated from its peel that has antagonist ability effectively in controlling anthracnose disease of banana fruit.  This research was started with an isolation of C. musae and yeast from banana, followed by in vivo assay with count percentage antagonist level, slide culture, and in vivo assay to know pathogen incubation stage and incident disease level. The yeast that isolated from ambon variety was Candida sp. and Pichia sp. Metchnikowia sp. from kepok variety respectively.
The Effectiveness Of Addition Mycorrhizae And Fertilizer Composition On The Spinach’s Growth (Amaranthus sp) ambar susanti; Andreas Abdul Aziz; Anton Muhibuddin
JURNAL AGRONOMI TANAMAN TROPIKA (JUATIKA) Vol 3 No 2 (2021): Volume 3 No.2 July 2021
Publisher : LPPM UNIVERSITAS ISLAM KUANTAN SINGINGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36378/juatika.v3i2.1396

Abstract

The production of organic spinach hasn’t been able to keep up with market demand, it is necessary to produce quantity and organic spinach. The research was carried out in the laboratory and greenhouse of the Agriculture Faculty, KH.A. Wahab Hasbullah University in February - May 2021. The purpose of this study was to aims to determine the effectiveness of giving mycorrhizae to spinach plants given various organic fertilizer compositions with the decomposer Trichoderma sp. The research design used was a completely randomized design, with five treatments and five replications. The treatments are; M0 (8 grams of mycorrhizae with 0% organic fertilizer as negative control), M1 (8 grams of mycorrhizae with 25% organic fertilizer), M2 (8 grams of mycorrhizae with 50% organic fertilizer), M3 (8 grams of mycorrhizae with fertilizer organic 75%), M4 (8 gr mycorrhizal with 5 grams of NPK fertilizer as positive control). The results are M2 treatment showed good growth in plant height (18.26 cm), the number of leaves (8.40 leaves), plant wet weight (3.65 gr), and root length (13.69 cm). Mycorrhizal spinach plants are effective in absorbing nutrients available in organic fertilizers 50% of the composition of the growing media, but less effective at 25%,75%, and NPK Mutiara 16:16:16 fertilizer. Organic fertilizers with local Trichoderma sp isolate decomposer are able to provide nutrients needed by spinach plants for their growth. The application of organic fertilizers with a composition of 50% to plants given mycorrhizae is possible to suppress the use of inorganic fertilizers.
Potensi Alang-alang (Imperata cylindrica (L.) Beauv) dalam Produksi Etanol Menggunakan Bakteri Zymomonas mobilis Sevy Dwi Kartikasari; Sri Nurhatika; Anton Muhibuddin
Jurnal Sains dan Seni ITS Vol 2, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (769.825 KB) | DOI: 10.12962/j23373520.v2i2.3741

Abstract

Sumber selulosa yang murah dan melimpah dapat diperoleh dari gulma alang-alang (Imperata cylindrica (L.) Beauv). Produksi etanol dari bahan baku selulosa alang-alang umumnya difermentasikan oleh yeast. Pada penelitian ini fermentasi dilakukan menggunakan bakteri Zymomonas mobilis. Penelitian dilakukan pada bulan November 2012 hingga Mei 2013 di laboratorium Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, ITS. Penelitian bertujuan untuk mengetahui potensi alang-alang (I. cylindrica (L) Beauv.) dalam produksi etanol menggunakan bakteri Z. mobilis pada konsentrasi inokulum dan waktu fermentasi yang optimum. Penelitian ini dilakukan dalam tiga langkah utama yaitu pretreatment substrat, hidrolisis enzimatik oleh Penicillium sp., dan fermentasi etanol oleh Z. mobilis. Fermentasi dilakukan menggunakan berbagai perlakuan konsentrasi inokulum (0, 5, 10, dan 15%) dan lama fermentasi (0, 3, 5, 7, dan 9 days) serta dianalisa menggunakan uji Analysis of Varians (ANOVA). Masing-masing perlakuan dilakuan 2 kali pengulangan, sehingga diperoleh 40 unit percobaan dengan parameter yang diamati adalah kadar etanol. Hasil penelitian menunjukkan bahwa alang-alang (I. cylindrica (L.) Beauv.) berpotensi untuk dijadikan sebagai bahan baku produksi etanol dengan konsentrasi etanol tertinggi diperoleh dari interaksi antara 10% konsentrasi inokulum Z. mobilis dan waktu fermentasi 7 hari yaitu sebesar 9,02 % (v/v)
The Potential of Endophytic Fungi in Promoting Rice Plant Growth and Suppressing Blast Disease Novia Dwi Putri; Anton Muhibuddin; Luqman Qurata Aini
Journal of Tropical Plant Protection Vol 2, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.2.2

Abstract

Endophytic fungi are fungi that inhabit the internal tissues of plant and belong to a group of microorganisms reported to have beneficial effects on plants. In this study, three endophytic fungi isolated from rice plant tissue were identified as Trichoderma asperellum, Curvularia chiangmaiensis, dan Fusarium solani. These three fungi have the ability to produce IAA between 3.03 to 6.00 µg/mL. For phosphate solubility assay, all endophytic fungi showed a clear zone around the colonies in Pikovskaya medium and could dissolve phosphate in the range of 2.74 to 17.61 µg/mL. In addition, in vivo observations of the inoculation endophytic fungi can reduce the intensity of blast disease in rice plant. So that, it can be seen that the three fungal isolates can become plant growth promoting fungi and can also used as bicontrol agents for blast disease in rice plant.
Co-Authors Abdul Cholil Abdul Latief Abadi Ahmad Ilham Tanzil Ahmad Ilham Tanzil Akhmat Riza Faizah ambar susanti Andik Setiawan Andreas Abdul Aziz Ari Kristini AthoÂ’illah Ahmad Athoillah Ahmad Birtha Niken Pratiwi Birtha Niken Pratiwi Chintya Ivana Situmorang Dhani Galih Rahmawanto Dhani Galih Rahmawanto Dhewyangga Bismi Panglipur DIAN WULANDARI Dian Wulandari Edi Priyo Utomo Edi Priyo Utomo Ernita Lovera Pratiwi Pasaribu Goetz, Peter Ika Rocdjatun Sastrahidayat Ika Rochdjatun Sastrahidayat Ika Rochdjatun Sastrahidayat Ika Rochjatun Ina Mustika Dewi Istiqomah Istiqomah istiqomah istiqomah Liliek Sulistyowati Liliek Sulistyowati Luaili Addina LuÂ’aili Addina Luqman Qurata Aini Luqman Qurota Aini Luqman Qurota Aini Muhammad Akhid Syibli Nasir Saleh Novia Dwi Putri Nur Musyarrofatul Mala NURUL HIDAYAH Pramudita, Oki Punnapayak, Hunsa Ria Kurniasih Ria Kurniasih Rizkiana Intan Pratiwi Rizkiana Intan Pratiwi Rosy Husna Shofiana Rosy Husna Shofiana Sektiono, Antok Wahyu Sevy Dwi Kartikasari Sholihah, Dewi Maratus Sifaunajah, Agus Sri Nurhatika Syamsuddin Djauhari Syamsuddin Djauhari Syamsuddin Djauhari Syamsuddin Djauhari Virgien Margi Nirwana Wahyu Astiko Wibowo, Kartika Novitasari