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All Journal MAJALAH ILMIAH PEMBELAJARAN Jurnal Penelitian Saintek INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni EKSAKTA: Journal of Sciences and Data Analysis Jurnal Sains Dasar Jurnal Matematika & Sains Indonesian Journal of Biotechnology Indonesian Journal of Science and Technology Indonesian Journal of Chemistry Molekul: Jurnal Ilmiah Kimia

Retno Arianingrum

Jurusan Pendidikan Kimia, FMIPA, Universitas Negeri Yogyakarta

Author-ID : 231928

Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics

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Silikat dan Titanium Silikat Mesopori-Mesotruktur Berbasis Struktur Heksagonal dan Kubik Hari Sutrisno; Retno Arianingrum; Ariswan Ariswan
Jurnal Matematika & Sains Vol 10, No 2 (2005)
Publisher : Institut Teknologi Bandung

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Silicalite (MCM-41 & MCM-48) and titanium silicalite (Ti-MCM-41 & Ti-MCM-48) mesoporous-mesostucture material have been synthesized and studied. Its have been obtained from hydrolysis and condensation of reactant : tetraethyl orthosilicate (TEOS) as silicium source and cetyltrimethyl ammonium bromide (CTAB) surfactant as template, NaOH as base and water solution. Ti-MCM-41 & Ti-MCM-48 mesoporous-mesostucture material have been synthesized from crystal of [Ti8O12(H2O)24]Cl8.HCl.7H2O as titanium source. Surfactant was removed by calcinations at 5500C for 5 hours. MCM-41 and Ti-MCM-41 are crystallized on the hexagonal structure, plane group p6 with unit cell parameter: a = 44.09 and 49.48 Çº respectively, meanwhile MCM-48 and Ti-MCM-48 are crystallized on the cubic structure, space group Ia3d with unit cell parameter: a = 87.46 and 94.42 Çº respectively.

IDENTIFICATION AND BIOLOGICAL ACTIVITY TEST SOME ISOLATED COMPOUNDS FROM STEM BARK OF MELINJO (GNETUM GNEMON) Atun, Sri; Arianingrum, Retno; Masatake, Niwa
Jurnal Sains Dasar Vol 1, No 1 (2012): April 2012
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Isolation and structure elucidation of two compounds, namely resveratrol (1), and 3- methoxyresveratrol (2) from stem bark of Melinjo (Gnetum gnemon) had been done. The isolation of those compounds was carried out by chromatographyc method and structure elucidation was performed by interpretation of spectroscopic data, including UV, IR, 1H and 13C NMR 1D and 2D, and FABMS. The result of this study showed that activity each compounds as radical hydroxyl scavenger of resveratrol (1), and 3-methoxyresveratrol (2), with an IC50 45,17 and 60,12; g/ml respectively. Each compound showed significant activity as UV-B protection. Activity test as UV-B protection showed that resveratrol and methoxyresveratrol have maximum protections (SPF 8,03 and 12,34 respectively), each compounds on 50 g/ml. Key word : melinjo; Gnetum gnemon; natural antioxidant; UV-B protection

Synergistic effects of para-hydroxy meta-methoxy chalcone (pHmMC)- doxorubicin treatments on T47D breast cancer cells Retno Arianingrum; Retno Sunarminingsih; Edy Meiyanto; Sofia Mubarika
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Resistance to some cancer chemotherapeutic drugs has been identifed. One strategy to overcome that problem is by combining two or more of the drugs to get co-chemotherapeutic effects. A derivate chalcone, 3 - (4’-hydroxy-3’-methoxyphenyl)-1-phenyl-2-propene-1-on or para hydroxy meta methoxy chalcone (pHmMC), has been reported to have cytotoxic activity on some cancer cells through some pathways. The aim of this study was to investigate the effects of combinations of pHmMC and Doxorubicin (Dox) on the cytotoxicity, anti-proliferation, apoptosis, and the cell cycle of T47D (breast cancer cell-lines) in vitro. The cytotoxic and antiproliferative activity were determined by MTT (3-[4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] assay. The combination index (CI) was used to determine the synergistic, additive or antagonistic effects of the combinations. Flowcytometry method was performed to determine the combination effects on the apoptosis and cell cycle. The results indicated that the combinations had a higher inhibitory effect on the cell growth compared to those of single treatments of pHmMC and Dox. All the doses used in the combinations were lower of the single doses at their IC50s. The results showed all the combinations gave synergistic (CI: 0.3 – 0.7) up to strong synergistic (CI: 0.1 – 0.3) effects. The synergistic effects of the combinations were due to increased apoptosis and induced cell cycle arrest in S and G2/M phases on the cancer cell lines.

PENGARUH MEDIA TERHADAP EFEKTIVITAS PEMBELAJARAN PRAKTIKUM BIOKIMIA Eddy Sulistyowati; Retno Arianingrum; Togu Gultom; Das Salirawati
MAJALAH ILMIAH PEMBELAJARAN No 2 (2006): Jurnal Majalah Ilmiah Pembelajaran Edisi Oktober 2006
Publisher : MAJALAH ILMIAH PEMBELAJARAN

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The objectivity of this research is to determine media that effective to studying in biochemistry practical work to improve student achievement.The sample of this research is all students of biochemistry practical work at odd semester in 2003/2004 in Chemistry Department in Faculty of Mathematics and Natural Science, at Yogyakarta State University. The student’s number was 28 people. The research was carried out using 3 media, i.e. Over Head Transparent presented by Over Head projector (OHP) at carbohydrate topic; Power Point presented by LCD projector at protein topic, and Audio-Visual presented by LCD projector at enzyme topic. The instruments of this research are observation sheet, pre-test and post-test, and evaluation questionnaire. The increase of post-test to pre-test was evaluated using t-test. The analyzed of observation data was carried out by qualitative descriptive using calculation percentage continued by qualitative analyzed. The mean of observation data was analyzed by ANAVA-A test followed by LSDT test. The data of evaluation questionnaire was calculated as percentage of student choice at this questionnaire.The result of observation data showed that there was the increasing in practical work skilled using OHT, Power Point, and Audio Visual media. There were 18.16 (very low), 36.39 (medium); and 49.31 (very high) respectively. The ANAVA-A test and LSDT indicated that there was significant difference between three score of the practical work skilled with three different media at three topics. The result of pre-test and post-test showed that there was the increasing score for all topics. The great increasing was enzyme topic when using Audio-visual media. The t-test indicated that there was the differentia of the increase score at three topics. The evaluation questionnaire showed that Audio-Visual media presented by LCD was effective

EFFECT OF TIME FERMENTATION OF SOYBEAN TEMPE TO TRIPSIN ACTIVITY Retno Arianingrum; Eddy Sulistyowati; Das Salirawati
Jurnal Penelitian Saintek Vol 12, No 2: Oktober 2007
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The aim of the research is to study the effect of the duration of fermentation process toward the content of soluble protein in tempe from soybean, and the activity of trypsin enzym toward that protein.The content of soluble protein was analyzed by Lowry method using casein as solution standard, and the tripsin activity was determined by Anson method. The duration offermentation process were 0,24,48,72, 96, and 120 hours. The research indicated that the content ofsoluble protein in tempe duringfermentation process for 0, 24, 48, 72,96, and 120 hours were 0.172; 0.212; 0.217; 0.212; 0.197, and0.158% (wlw) respectively, and the activity of trypsine toward the that soluble protein were 1.35; 2.33; 2.73; 2.13; 1.17 and o. 78 unit. It was indicated that the duration of fermentation process influenced thecontent of soluble protein and tripsin activity. The highest of trypsin activity was infermentation process for 48 hours.

EFFECT OF MALELO SANDAL SALT WITH HN03 TO THE EFFICIENCY OF CRYSTAL IMPLEMENTATION Siti Sulastri; Susila Kristianingrum; Retno Arianingrum
Jurnal Penelitian Saintek Vol 9, No 1: April 2004
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The objective of this study was to investigate the effect of soaking nitrate acid of Malelo sand to adsorption efficiency of chromium (Cr). The Malelo sand was prepared by soaking in nitric acid with variation of concentration were 17,5: 35 and 70% for 24 hours at room temperature. The adsorption efficiency of chromium was determined based on concentration of chromium residue after adsorption. which measured using Atomic Absorption Spectrophotometer (AAS). The characterization of Malelo sand determined using FTIR and XRD. The result indicated that the mean ofadsorption efficiency of chromium on Malelo sand without preparation and soaking in nitrate acid with variation concentration 17.5: 35 and 70% were 91,36: 92,40: 92,72 and 94,004% respectively.

PEMANFAATAN LIMBAH KULIT lKAN MENJADI KERUPUK (RAMBAK) Susila Kristianingrum; Retno Arianingrum; Siti Sulastri
INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni Vol 10, No 1 (2006): Februari 2006
Publisher : LPPM UNY

This program of implementation Science, Technology and Arts (Ipteks) intended to introduce and give skill training about making chips from fish skins (kerupuk kulit ikan) to the community in Tanjungsari Sub district, Gunungkidul Regency. In this area, there are plenty of fish skins that can pollute and disturb the beauty of the environment. Producing chips from fish skins can reduce the pollutant and increase the value of fish skins.The program was held by giving information and demonstration methods in two locations with different participants. The first program was held in Tanjungsari Sub-district Hall for 35 participants, who were village chiefs' wives. The second program was held at Baron beach hall to accommodate 20 participants, consisting of fishermen's wives and members of youth group.The questionnaire result of first program showed that 85.71 % of theparticipants considered this program profitable and 74.28% said that they were interested to develop business in producing fish skin chips. All the participants (100%) of the second program said that the program was profitable and they were interested to develop business in producing fish skin chips. Thus, the program succeeded in broadening the participants' perspectives and skills on how to make use of waste, turning it into fish skin chips and to motivate the participants to develop business using the knowledge and skills already obtained.Key words:fish skin waste.fish skin drips

Comparison on Isolation Technique of Vaninilin from Vanili Fruit (Vanilla planifolia Andrews Winarto Haryadi; Sri Handayani; Retno Arianingrum
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 11, ISSUE 2, August 2010
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

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Isolation of vanilin from vanili fruit by two method; maseration and Soxhlet using ethylacetate, ethanol and chloroform solvents has been investigated. Extraction result was hydrolized using sodium hydroxide by acidification using chloride acid. Filtrate was extracted by using chloroform and the targeted vanilin was separated by evaporation. Putrification was enganged by rechrystallization using aquadest-ethanol. Elucidation to the compound structure was performed by FTIR spectrophotometer, H-NMR, C-NMR, HMQC and HMBC. It is noted that vanilin isolation by soxhletazion procedure was more effective compared to maseration. Ethylacetate was more effective solvent than chloroform and conversely ethanol is inefective for the isolation. Keywords: vanilin isolation, soxhlet isolation, maseration

Characterization of Curcuminoid from Curcuma xanthorrhiza and Its Activity Test as Antioxidant and Antibacterial Sri Atun; Nurfina Aznam; Retno Arianingrum; Senam Senam; Bian Ihda An Naila; Astuti Lestari; Nur Aini Purnamaningsih
Molekul Vol 15, No 2 (2020)
Publisher : Universitas Jenderal Soedirman

Curcuminoids are the main component found in many Zingiberaceae family plants. The aim of this study was to characterize curcuminoid and its activity test as an antioxidant and antibacterial. Dryed powder of C. xanthorrhiza (1 kg) was macerated with ethanol for 24 hours at room temperature. Ethanol extract of C. xanthorrhiza was subsequently fractionated with n-hexane and chloroform to take the yellow or orange indicated contain of curcuminoids. Analysis of total phenolic levels was carried out by the Follin-Ciaocalteau method. The isolation of curcuminoid componens from this fraction was carried out by chromatographic method and the structure elucidation was performed by interpretation of spectroscopic data, including UV, IR, 1H and 13C NMR 1D and 2D. The antioxidant activity test used the DPPH (2,2-diphenylpicrylhydrazyl) method, while the antibacterial activity test used Kirby Bauer test diffusion method. The results showed that the curcuminoid fraction yield was 10.06% from ethanol extract C. xanthorrhiza. The total phenolic content of curcuminoids fraction was 745.45 ± 18.5 mg galic acid (GA)/g extract. Curcuminoids fraction was isolated a known compound desmethoxycurcumin (1). The content of demethoxycurcumin (1) in curcuminoid fraction is 20.97 %.The antioxidant activity of curcuminoids fraction showed strongest activity with IC50 24.98 µg/ml. Antibacterial activity against of the four pathogenic bacteria showed medium activity. The study suggests that curcuminoids extract from C. xanthorrhiza rhizome have potential compounds could be suitable for antioxidant and the treatment of various infections caused of microbial.

BALANOCARPOL AND AMPELOPSIN H, TWO OLIGORESVERATROLS FROM STEM BARK OF Hopea odorata (DIPTEROCARPACEAE) Sri Atun; Nurfina Aznam; Retno Arianingrum; Masatake Niwa
Indonesian Journal of Chemistry Vol 6, No 3 (2006)
Publisher : Universitas Gadjah Mada

Two oligoresveratrol, namely balanocarpol (2) and ampelopsin H (3) had been isolated from the steam bark of Hopea odorata (Dipterocarpaceae). The structures of these compounds were elucidated based on physical and spectroscopic data (MS, 1H and 13C NMR 1D and 2D). The activity of these compounds was evaluated against the 2-deoxyribose degradation induced by the hydroxyl radical generated via a Fenton-type reaction. The result showed that activity each compounds as radical hydroxyl scavenger of balanocarpol, and ampelopsin H with an IC50 1802.3 and 4840.0 μg/mL, respectively. Each compound showed low activity. Vitamin C (IC50 83.9 μg/mL) and butylated hydroxyl toluene (1328.0 μg/mL) were used as positif controls. These results suggest that oligoresveratrols from stem bark of H. odorata may be useful as potential sources of natural antioxidants.

Co-Authors Alya Rizkita Irwan Ariswan Ariswan Astuti Lestari Bian Ihda An Naila Das Salirawati Das Salirawati Eddy Sulistyowati Eddy Sulistyowati Edy Meiyanto HARI SUTRISNO Latifah Kurnia Devani Masatake Niwa Nadiya Fitri Anisa Nida Qurbaniah Juhara Niwa Masatake Nur Aini Purnamaningsih Nurfina Aznam Retno Sunarminingsih S. Senam Senam Senam Siti Sulastri Sofia Mubarika H Sri . Handayani Sri Atun Sri Atun Sri Atun SRI ATUN Susila Kristianingrum Togu Gultom Winarto Haryadi

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