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The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2-Deoxyguanosine Formation in Human Skin Arief Budiyanto, Irianiwati, Catharina Sagita Yohanes Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 02 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Background: Ultraviolet B (UVBI may cause DNA damage due to photooxydative reaction and form 8hydroxy-2-deoxyguanosine (8-0HdGI as a genotoxic product. Topically extra virgin olive oil (EVOOI can decrease 8-0HdG formation among UVB irradiated-hairless mice epidermal cells. Such effect in human skin has never been discovered.Objective: To know the effect of topically EVOO in UVB induced-8-0HdG formation in human epidermal cells.Method: A simple experiment was performed on cultured human foreskins. The foreskins were divided into 3 groups: group was treated with 100mJ/cm2 UVB + topical EVOO, group B with 100mJ/cm2 UVB, and group C as control. All skin was cultured in completed DMEM and fixation was performed at one and 24 hours. 8-0HdG expression was determined immunohistochemically with antibody anti 8-0HdG and measured based on histogram of selected colour of Adobe photoshop.Results: The mean of 8-0HdG expression in experimental groups: (8.02:t 13.81, (26.95:t 23.231. and 14.96:t 7.141, respectively (p =0.0001 at an hour; (14.91 :t30.63I, (37.58:t 25.631. and (8.76:t 10.941 respectively (p = 0.0001 at 24 hours.Conclusion: Extra virgin olive oil was proved to reduce the 8-0HdG formation in exposed human skin-UVB. and may be developed to be anti-photocarcinogenesis active ingredient topically.Kata kunci:Extra virgin olive oil, UVB, 8-OHdG, human Skin

Targeted phototherapy for skin diseases Arief Budiyanto, SaÃ¢Â€Â™adatul Huriyah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Phototherapy is a therapeutic strategy in dermatology for treating several skin diseases.Conventional phototherapy has some disadvantages, hence a targeted phototherapy that emitsselective laser or ultraviolet (UV) light and targets specific area of affacted skin has been developed.Targeted phototherapy is considered to be more aggressive and has higher efficacy. Severaltargeted phototherapy devices emit one or several types of light. The use of targeted phototherapyhas been studied in vitiligo, psoriasis, eczema, keloid, hypopigmented scar or striae alba, andoral lichen planus.

The effect of topically extravirgin olive oil on the UVB-induced immunosuppression AriefBudiyanto, Irianiwati, Vohanes Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 03 (2009)
Publisher : Universitas Gadjah Mada

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Background: UVBradiation may act as an immunosuppressive agent through Langernans cells (LCs)depletion correlated with cyclobuthane pyrimidine dimer (CPD), as the most mutagenic photoproducts. Other studies showed that olive oil can prevent various human cancers, which are defect of immune-surveillance. The effect of olive oil in the UVBinduced LCs depletion is still unclear. Objective: To discover the topical effect of extravirgin-olive-oil in the LCs depletion. Methods: A simple experimental study was performed on foreskins collected from 8 circumcised boys. Each of them was cut into 3 pieces of 0.5 cm2, grouped randomly to: (Olive oil+ UVB)group, (placebo + UVB)group, and unirradiated group. They were cultured in DMEM for 24 hours, irradiated with 100mJs/cm2 UVB, and olive oil or placebo were then applied immediately after irradiation. All specimens were fixed in buffered-formalin, paraffin blocked, and cut to be 2 slides, and each of them was stained with anti-CD 1a or anti-CPD antibody. The pixels of AEC signats were digitally counted based on Photoshops selected color histogram.The pixel means of various groups were analyzed by Kruskal-Wallis test and post hoc analysis by Mann Whitney test. Results: Olive oil treated groups showed a very significant (P

Pure Neural Leprosy Verdy, Verdy; Budiyanto, Arief; Siswati, Agnes Sri
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Faculty Of Medicine Airlangga University

Background: Pure neural leprosy (PNL) is an infection by Mycobacterium leprae with clinical manifestation as nerve thickening, sensory nerve impairment, with or without motoric nerve involvement, and without skin lesion. Pure neural leprosy causes progressive degeneration of nerve function. Diagnosis establishement of PNL is difficult. Purpose: To discuss about PNL especifically in aspects of the diagnosis and therapy. Reviews: Gold standard in diagnosis of PNL is histopathological examination from nerve biopsy but this procedure is still difficult for various reasons. Some investigations can be done for PNL such as cytological fine needle aspiration, Mitsuda test, serology test for antibody anti-PGL1, electroneuromyography (ENMG), and polymerase chain reaction (PCR). Pure neural leprosy classification is based on findings of abnormality in neurological, immunological, and histopathological. Pure neural leprosy therapy is based on the classification of paucibacillary or multibacillary. Conclusions: The clinical and epidemiological findings plus with the electroneuromyography alteration were finally accepted as sufficient criteria to diagnose PNL. Pure neural leprosy therapy follows WHO regiment in accordance with the paucibacillary or multibacillary classification.Key words: pure neural leprosy, Mycobacterium leprae, nerve biopsy, anti-PGL-1, electroneuromyography.

Successful Treatment of Actinomycetoma with Combination of Cotrimoxazole and Tetracycline Verdy, Verdy; Dewi, Vina Ajeng Puspa; Budiyanto, Arief; Siswati, Agnes Sri
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Faculty Of Medicine Airlangga University

Background: Actinomycetoma is an infection of chronic granulomatous disease on the skin, subcutaneous tissue, fascia, and bone, occured after trauma because of bacteria innoculation. Clinical features seem painless nodules, abscess, fistula, and sinusÂ with granulated discharge.Gold standard treatment for actinomycetoma is cotrimoxazole. Combination therapy with other antibiotic is needed to prevent resistance and improve therapeutic efficacy. Purpose: To discuss successful treatment of actinomycetoma with combination of cotrimoxazole and tetracycline Case: A 19-years-old woman had complained ofÂ chronic post-traumatic wounds since two years ago. Dermatological examination showed multiple painless nodules, sinus, and discharge on right dorsum pedis. Histopathological examination demonstrated sinus in dermis, suppurative granulomas, and SplendoreHoeppli phenomenon in dermis layer. Case Management: Combination therapy consist of cotrimoxazole 2x960 mg, that was given for 6 months and tetracycline 4x500 mg, that was given in the first 1 month.Â No side effects and drug allergy during and/or post treatment was found. After clinical evaluation for 6 months, successful of treatmentÂ was obtained and no recurrence was found. Conclusion: The combination therapy of cotrimoxazole for 6 months and tetracycline for 1 month was proven to be effective and no recurrence was found.Key words: actinomycetoma, cotrimoxazole, tetracycline.

Pathogenesis, evaluation, and recent management of diabetic foot ulcer Wijaya, Lorettha; Budiyanto, Arief; Astuti, Indwiani; Mustofa, .
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Diabetic foot ulcers (DFUs) are a major health problem as the number of patients continues to increase, are difficult to heal, require enormous management costs, and deteriorate the quality of life of patients, their families and societies. The pathogenesis of DFUs is complex. Most important factors that increase the risk of DFUs are peripheral neuropathy, foot deformities, frequent minor trauma, and peripheral arterial disease. Neuropeptides derangement, hypoxia, hyperglycemia, and infection act as the cause of chronicity of DFUs. Therefore, during the initial evaluation of DFU, patients need to be checked for their metabolic status, presence or absence of peripheral neuropathy, vascular insufficiency, foot deformities, and infection of the ulcer and its underlying bone. Then, DFUs are classified by the severity of vascular insufficiency, the depth of the wound, and the severity of the infection. This classification system helps clinicians to determine whether the patient needs to be hospitalized or amputated and helps to establish DFU management strategies. In the management of DFUs, adequate blood flow to the wound area should be achieved. Glycemic control and standard wound care should be encouraged. Standard wound care includes debridement, offloading, wound moisture balance with suitable dressing, edema control, and infection control. Education about preventive foot care should be taught to the patients and their families. As the pathogenesis and management of DFUs are complex, a multidisciplinary team consists of expert individuals in their respective fields should be involved.

Pure Neural Leprosy Verdy Verdy; Arief Budiyanto; Agnes Sri Siswati
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 27 No. 1 (2015): BIKKK APRIL 2015
Publisher : Faculty of Medicine, Universitas Airlangga

Background: Pure neural leprosy (PNL) is an infection by Mycobacterium leprae with clinical manifestation as nerve thickening, sensory nerve impairment, with or without motoric nerve involvement, and without skin lesion. Pure neural leprosy causes progressive degeneration of nerve function. Diagnosis establishement of PNL is difficult. Purpose: To discuss about PNL especifically in aspects of the diagnosis and therapy. Reviews: Gold standard in diagnosis of PNL is histopathological examination from nerve biopsy but this procedure is still difficult for various reasons. Some investigations can be done for PNL such as cytological fine needle aspiration, Mitsuda test, serology test for antibody anti-PGL1, electroneuromyography (ENMG), and polymerase chain reaction (PCR). Pure neural leprosy classification is based on findings of abnormality in neurological, immunological, and histopathological. Pure neural leprosy therapy is based on the classification of paucibacillary or multibacillary. Conclusions: The clinical and epidemiological findings plus with the electroneuromyography alteration were finally accepted as sufficient criteria to diagnose PNL. Pure neural leprosy therapy follows WHO regiment in accordance with the paucibacillary or multibacillary classification.Key words: pure neural leprosy, Mycobacterium leprae, nerve biopsy, anti-PGL-1, electroneuromyography.

Successful Treatment of Actinomycetoma with Combination of Cotrimoxazole and Tetracycline Verdy Verdy; Vina Ajeng Puspa Dewi; Arief Budiyanto; Agnes Sri Siswati
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 27 No. 1 (2015): BIKKK APRIL 2015
Publisher : Faculty of Medicine, Universitas Airlangga

Background: Actinomycetoma is an infection of chronic granulomatous disease on the skin, subcutaneous tissue, fascia, and bone, occured after trauma because of bacteria innoculation. Clinical features seem painless nodules, abscess, fistula, and sinus with granulated discharge.Gold standard treatment for actinomycetoma is cotrimoxazole. Combination therapy with other antibiotic is needed to prevent resistance and improve therapeutic efficacy. Purpose: To discuss successful treatment of actinomycetoma with combination of cotrimoxazole and tetracycline Case: A 19-years-old woman had complained of chronic post-traumatic wounds since two years ago. Dermatological examination showed multiple painless nodules, sinus, and discharge on right dorsum pedis. Histopathological examination demonstrated sinus in dermis, suppurative granulomas, and SplendoreHoeppli phenomenon in dermis layer. Case Management: Combination therapy consist of cotrimoxazole 2x960 mg, that was given for 6 months and tetracycline 4x500 mg, that was given in the first 1 month. No side effects and drug allergy during and/or post treatment was found. After clinical evaluation for 6 months, successful of treatment was obtained and no recurrence was found. Conclusion: The combination therapy of cotrimoxazole for 6 months and tetracycline for 1 month was proven to be effective and no recurrence was found.Key words: actinomycetoma, cotrimoxazole, tetracycline.

Pengaruh Media Pembelajaran dan Kemandirian Siswa terhadap Hasil Belajar Mata Pelajaran PPKn SMK Via Medika Ciputat Budiyanto, Arief; Imbron, Imbron; Zaman, M. Badru
JIIP - Jurnal Ilmiah Ilmu Pendidikan Vol. 4 No. 3 (2021): JIIP (Jurnal Ilmiah Ilmu Pendidikan)
Publisher : STKIP Yapis Dompu

Tujuan dari penelitian ini adalah mengetahui mengenai pengaruh dari pengaruh media pembelajaran dan kemandirian siswa terhadap hasil belajar mata pelajaran PPKn siswa. Metode dalam penelitian ini adalah menggunakan pendekatan kuantitatif dengan survey sebagai instrument penelitian. Data dikumpulkan dari 62 responden yang dijadikan sebagai sample jenuh. Hasil dari penelitian ini adalah media pembelajaran tidak berpengaruh terhadap hasil belajar mata pelajaran PPKn siswa hal tersebut dibuktikan dengan nilai Thitung yang lebih kecil dari Ttabel (-0,350<1,667). Sedangkan kemandirian siswa dalam penelitian ini, hal tersebut dibuktikan dengan nilai Thitung yang lebih besar dari Ttabel (4,189>1,667) dengan tingkat signifikan lebih kecil dari 0,05 (0,00<0,05) yang berarti sangat signifikan. Dan untuk hasil uji simultan pengaruh media pembelajaran dan kemandirian siswa terhadap hasil belajar mata pelajaran PPKn siswa terbukti berpengaruh, hal tersebut dibuktikan melalui nilai Fhitung yang lebih besar dari Ftabel (13,497>3,15) dengan tingkat signifikan (0,00<0,05) yang berarti sangat signifikan.

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