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Characterization of Crude Protease Bacillus sp 31 Utarti, Esti; Nurita, Lina; Arimurti, Sattya
Jurnal ILMU DASAR Vol 10 No 1 (2009)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Bacillus sp 31 was bacteria which produce protease. Characterization of protease from Bacillus sp 31 i.e. pH, temperature, influence of metal ion, enzyme kinetic and enzyme termostability is important to get optimal enzyme activity. Protease activity showed values 146.40 U/ml on pH 9 and optimal temperature 60°C by value. Protease activity increased by addition of 159.50 U/ml Fe2+, but its activity decreased by addition of Mg2+, Cu2+, Ca2+, Al2+, Zn2+ dan Mn2+. Maximal velocity (Vmax) of enzyme-catalysed reactions was 21.32 U/ml with Km 1.5x10-3 mg/ml (Michaels-Menten Kinetic). Protease was very stable at 60°C for 4 hours of incubation and 7 hours of half-time.
SKRINING BAKTERI SELULOLITIK ASAL VERMICOMPOSTING TANDAN KOSONG KELAPA SAWIT Azizah, Siti Nur; Muzakhar, Kahar; Arimurti, Sattya
BERKALA SAINSTEK Vol 2, No 1 (2014)
Publisher : My Home

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Abstract

Biomassa Tandan Kosong Kelapa Sawit (TKKS) dihasilkan dalam jumlah melimpah selama pemanenan, sehingga harus didekomposisi dalam waktu singkat. Melalui vermicomposting, TKKS dikonversi menjadi kompos yang berlangsung selama 2-3 bulan, sehingga untuk mempercepat proses dekomposisi, penelitian ini perlu dilakukan. Lima puluh satu isolat bakteri selulolitik berhasil diisolasi dari vermicomposting limbah TKKS. Hasil uji pada media CMC (Carboxymethyl Cellulose) plate, empat isolat memiliki aktivitas selulolitik tertinggi, yaitu isolat 20, 40a, 40b dan 49 dengan indeks aktivitas sebesar 11,90; 10,97; 11,29, dan 11,24. Selama hidrolisis menggunakan substrat CMC dan TKKS, isolat 20 mampu memproduksi gula reduksi tertinggi yaitu sebesar 12,27 μg/mL dan 49,31 μg/mL, sedangkan isolat 40a, 40b, dan 49 sebesar 3,48 μg/mL, 6,28 μg/mL dan 3,10 μg/mL di substrat CMC dan sebesar 24,83 μg/mL, 11,21 μg/mL dan 8,25 μg/mL di substrat TKKS. Keempat isolat bakteri termasuk bakteri Gram negatif dengan bentuk sel batang. Kata Kunci: bakteri selulolitik, gula reduksi, vermicomposting TKKS.
KARAKTERISASI ISOLAT BAKTERI FIBRINOLITIK WU 021055* ASAL PERAIRAN PANTAI PAPUMA, JEMBER Sri Pananjung, Ajeng Maharani; Ulfa, Evi Umayah; Senjarini, Kartika; Arimurti, Sattya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 2, No 1 (2015): June 2015
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.11 KB) | DOI: 10.29122/jbbi.v2i1.528

Abstract

A blood clot (thrombus) in a blood stream is formed due to a circulatory system imbalance in the hemostasis which results in plug of blood vessels. The suppliy of nutrients and oxygen to the tissues is inhibited (ischemia) by the accumulation of thrombus and embolus in the blood vessel. This prosses is the main cause for further atherotrombotic diseases such as myocardial infraction and cerebral infraction. This disease could be overcome by thrombolytic therapy by using fibrinolytic protease enzyme. Fibrinolytic activity of protease enzymes have been studied from various species of bacteria. Bacterial isolate of WU 021055* obtained from Papuma coastal waters has demonstrated fibrinolytic activity. This research was aimed to identify the bacterial isolate through morphological characterization (colony and cell morphology), physiological characterization (indole test, carbohydrates fermentation test (glucose, lactose, sucrose and fructose), catalase test, starch hydrolysis test, and the pH effect test), and molecular identification using 16S rRNA. Based on those characterizations, the bacterial isolate of WU 021055* shows a high similarity to Bacillus aerius.Keywords: Atherotrombosis, fibrinolytic, identification, characterization, bacteria ABSTRAKBekuan darah (trombus) dalam peredaran darah terbentuk akibat ketidakseimbangan sistem sirkulasi dalam hemostasis yang menyebabkan penyumbatan pembuluh darah. Akumulasi trombus dan embolus pada pembuluh darah mengakibatkan suplai nutrisi dan oksigen ke jaringan terhambat (iskemia) dan bahkan kematian jaringan (infark). Pembentukan ini merupakan etiologi dari penyakit aterotrombosis seperti infark miokard dan infark serebral. Penyakit akibat trombosis ini dapat diatasi dengan terapi trombolitik dengan enzim protease fibrinolitik. Aktivitas enzim protease fibrinolitik telah diteliti dari berbagai spesies bakteri. Isolat bakteri WU 021055* asal perairan pantai papuma tampak memiliki aktivitas fibrinolitik. Pada penelitian ini dilakukan identifikasi isolat bakteri melalui karakterisasi morfologi (morfologi koloni dan sel), karakterisasi fisiologis (uji indol, uji fermentasi karbohidrat (glukosa, laktosa, sukrosa dan fruktosa), uji katalase, uji hidrolisis pati, dan uji pengaruh pH), dan identifikasi secara molekuler menggunakan 16S rRNA. Berdasarkan karakterisasi morfologi, fisiologi, dan marker 16S rRNA, isolat bakteri WU 021055* menunjukkan kemiripan yang tinggi dengan Bacillus aerius.Kata kunci: Aterotrombosis, fibrinolitik, identifikasi, karakterisasi, bakteri
AKTIVITAS PROTEOLITIK DAN FIBRINOLITIK ISOLAT BAKTERI DARI PERAIRAN PANTAI PAPUMA KABUPATEN JEMBER Setiawan, Arif; Arimurti, Sattya; Senjarini, Kartika; Sutoyo, Sutoyo
BERKALA SAINSTEK Vol 4 No 1 (2016)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v4i1.4457

Abstract

Berbagai jenis bakteri dari perairan pantai dapat dieksplorasi sebagai sumber penghasil senyawa penting. Sejauh ini, bakteri dari Perairan Pantai Papuma Kabupaten Jember yang memiliki aktivitas proteolitik dan fibrinolitik belum dilaporkan. Tujuan penelitian ini adalah menguji aktivitas proteolitik dan fibrinolitik pada sejumlah isolat bakteri. Metode penelitian meliputi uji aktivitas proteolitik menggunakan Media Skim Milk Agar (SMA) terhadap 23 isolat bakteri dan uji aktivitas fibrinolitik menggunakan Media Fibrin pada isolat yang diketahui memiliki aktivitas proteolitik. Metode uji aktivitas dilakukan secara semikuantitatif. Sebanyak 11 isolat bakteri memiliki aktivitas proteolitik. Isolat bakteri WU 021012* memiliki indeks proteolitik tertinggi yaitu 4,3. Isolat bakteri yang fibrinolitik diperoleh sebanyak 3 isolat. Isolat bakteri WU 021055* memiliki indeks tertinggi sebesar 11.
SKRINING BAKTERI SELULOLITIK ASAL VERMICOMPOSTING TANDAN KOSONG KELAPA SAWIT Azizah, Siti Nur; Muzakhar, Kahar; Arimurti, Sattya
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Biomassa Tandan Kosong Kelapa Sawit (TKKS) dihasilkan dalam jumlah melimpah selama pemanenan, sehingga harus didekomposisi dalam waktu singkat. Melalui vermicomposting, TKKS dikonversi menjadi kompos yang berlangsung selama 2-3 bulan, sehingga untuk mempercepat proses dekomposisi, penelitian ini perlu dilakukan. Lima puluh satu isolat bakteri selulolitik berhasil diisolasi dari vermicomposting limbah TKKS. Hasil uji pada media CMC (Carboxymethyl Cellulose) plate, empat isolat memiliki aktivitas selulolitik tertinggi, yaitu isolat 20, 40a, 40b dan 49 dengan indeks aktivitas sebesar 11,90; 10,97; 11,29, dan 11,24. Selama hidrolisis menggunakan substrat CMC dan TKKS, isolat 20 mampu memproduksi gula reduksi tertinggi yaitu sebesar 12,27 μg/mL dan 49,31 μg/mL, sedangkan isolat 40a, 40b, dan 49 sebesar 3,48 μg/mL, 6,28 μg/mL dan 3,10 μg/mL di substrat CMC dan sebesar 24,83 μg/mL, 11,21 μg/mL dan 8,25 μg/mL di substrat TKKS. Keempat isolat bakteri termasuk bakteri Gram negatif dengan bentuk sel batang. Kata Kunci: bakteri selulolitik, gula reduksi, vermicomposting TKKS.
Deteksi Aktivitas Fibrinolitik Isolat Bakteri WU 021055* Asal Perairan Pantai Papuma Jember Menggunakan Zimografi Ulfa, Evi Umayah; Utarti, Esti; Afkarina, Izzay; Arimurti, Sattya; Senjarini, Kartika
Global Medical & Health Communication (GMHC) Vol 5, No 2 (2017)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v5i2.1914

Abstract

Bakteri merupakan sumber penting berbagai enzim termasuk enzim fibrinolitik. Enzim ini diperlukan untuk mendegradasi bekuan darah pada orang yang mengalami penyakit trombosis. Isolat bakteri WU 021055* asal Pantai Papuma Jember terbukti menghasilkan enzim fibrinolitik ekstraseluler. Penelitian ini bertujuan mengetahui ukuran protein yang memiliki aktivitas fibrinolitik dan mengidentifikasi karakteristik morfologi isolat WU bakteri WU 021055*. Penelitian ini dilakukan di Laboratorium Mikrobiologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jember pada April–Agustus 2014. Aktivitas fibrinolitik presipitat protein (PP) ditentukan menggunakan metode fibrin plate agar dan zimografi fibrin. Ekstrak protein kasar (EPK) dipanen pada jam ke-12 dan dipresipitasi menggunakan amonium sulfat 80%. Hasil uji aktivitas fibrinolitik menggunakan fibrin plate agar menunjukkan presipitat memiliki aktivitas fibrinolitik lebih besar dibanding dengan EPK. Dari hasil karakterisasi PP menggunakan sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) diperoleh 11 pita protein dengan ukuran 12–41 kDa. Berdasar atas hasil zimografi fibrin, pita protein dengan berat molekul 24 kDa yang memberikan aktivitas fibrinolitik. Protein dengan ukuran 24 kDa ini mampu mendegradasi substrat fibrin. Simpulan, isolat bakteri WU 021055* mengandung berbagai protein ekstraseluler, memiliki bentuk koloni bulat berwarna putih dan termasuk bakteri gram prositif berbentuk batang.DETECTION OF FIBRINOLYTIC ACTIVITY OF WU 021055* BACTERIAL ISOLATE FROM PAPUMA BEACH COASTAL JEMBER USING ZYMOGRAPHYBacteria were important resources for various enzymes including fibrinolytic enzymes. This enzyme is  capable of degrading fibrin clot in patient with thrombotic diseases. Bacterial isolate of WU 021055* from Papuma Beach Coastal Jember could secrete extracellular fibrinolytic enzymes. The objective of this reasearch was to determine the molecular weight of protein responsible for fibrinolytic activity and to identify morphologycal characterization of bacterial isolate of WU 021055*. This study was conducted at Laboratory of Microbiology, Faculty of Mathematics and Natural Sciences, Universitas Jember in April–August 2014. Fibrinolytic activity of precipitate protein (PP) was determined by using fibrin plate agar and fibrin zymography. Crude protein extract (CPE) was harvested at 12 hours and precipitated by 80% ammonium sulphates. The result of fibrinolityc activity determination showed that fibrinolytic activity of PP was higher than CPE. Protein characterization of PP by using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) obtained 11 different protein bands corresponds to value 12–42 kDa. Based on fibrin zymography, the 24 kDa protein might contribute to fibrinolytic activity due to degraded fibrin substrates. In conclusion, bacterial isolate of WU 021055* contained extracellular fibrin protein was white colony and gram positives bacilli able to degraded.
Characterization of Edamame Indigenous Rhizobia as a Candidate of Biofertilizer Arimurti, Sattya
Jurnal ILMU DASAR Vol 10 No 1 (2009)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.186 KB)

Abstract

Five isolates, named R1, R3, R4, R6 and R7, were successfully isolated from leguminous edamame nodules, and characterized as indigenous rhizobia bacteria. All isolates were grown in a YEMA medium containing antibiotics ampicillin, streptomycin, rifampicin, tetracycline, chloramphenicol or penicillin. Cultivation revealed that R3 can grow in a medium containing all antibiotics, but not for R1 when they grow in a medium containing rifampicin. R7 could not grow when the medium contain streptomycin and rifampicin. Furthermore, R4 and R6 only grow at medium containing tetracycline. it seemed that R1 and R3 are more resistant against some antibiotics comparing with others. When YEMA containing bromthymol blue 1% medium was used, R1 produced the yellowish acid and R3 produced blue alkali. R1 also utilized dulcitol and Lhistidin as carbon and nitrogen source. R3 utilize the carbon source from dulcitol but cannot utilize the nitrogen source from L-histidin. Base on these results above, it can be suggested that R1 and R3 identified as Rhizobium leguminosarum and Bradyrhizobium japonicum.
KARAKTERISASI ISOLAT BAKTERI FIBRINOLITIK WU 021055* ASAL PERAIRAN PANTAI PAPUMA, JEMBER Sri Pananjung, Ajeng Maharani; Ulfa, Evi Umayah; Senjarini, Kartika; Arimurti, Sattya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 1 (2015): June 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.11 KB) | DOI: 10.29122/jbbi.v2i1.528

Abstract

A blood clot (thrombus) in a blood stream is formed due to a circulatory system imbalance in the hemostasis which results in plug of blood vessels. The suppliy of nutrients and oxygen to the tissues is inhibited (ischemia) by the accumulation of thrombus and embolus in the blood vessel. This prosses is the main cause for further atherotrombotic diseases such as myocardial infraction and cerebral infraction. This disease could be overcome by thrombolytic therapy by using fibrinolytic protease enzyme. Fibrinolytic activity of protease enzymes have been studied from various species of bacteria. Bacterial isolate of WU 021055* obtained from Papuma coastal waters has demonstrated fibrinolytic activity. This research was aimed to identify the bacterial isolate through morphological characterization (colony and cell morphology), physiological characterization (indole test, carbohydrates fermentation test (glucose, lactose, sucrose and fructose), catalase test, starch hydrolysis test, and the pH effect test), and molecular identification using 16S rRNA. Based on those characterizations, the bacterial isolate of WU 021055* shows a high similarity to Bacillus aerius.Keywords: Atherotrombosis, fibrinolytic, identification, characterization, bacteria ABSTRAKBekuan darah (trombus) dalam peredaran darah terbentuk akibat ketidakseimbangan sistem sirkulasi dalam hemostasis yang menyebabkan penyumbatan pembuluh darah. Akumulasi trombus dan embolus pada pembuluh darah mengakibatkan suplai nutrisi dan oksigen ke jaringan terhambat (iskemia) dan bahkan kematian jaringan (infark). Pembentukan ini merupakan etiologi dari penyakit aterotrombosis seperti infark miokard dan infark serebral. Penyakit akibat trombosis ini dapat diatasi dengan terapi trombolitik dengan enzim protease fibrinolitik. Aktivitas enzim protease fibrinolitik telah diteliti dari berbagai spesies bakteri. Isolat bakteri WU 021055* asal perairan pantai papuma tampak memiliki aktivitas fibrinolitik. Pada penelitian ini dilakukan identifikasi isolat bakteri melalui karakterisasi morfologi (morfologi koloni dan sel), karakterisasi fisiologis (uji indol, uji fermentasi karbohidrat (glukosa, laktosa, sukrosa dan fruktosa), uji katalase, uji hidrolisis pati, dan uji pengaruh pH), dan identifikasi secara molekuler menggunakan 16S rRNA. Berdasarkan karakterisasi morfologi, fisiologi, dan marker 16S rRNA, isolat bakteri WU 021055* menunjukkan kemiripan yang tinggi dengan Bacillus aerius.Kata kunci: Aterotrombosis, fibrinolitik, identifikasi, karakterisasi, bakteri
Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria Luthfiah, Luthfiah; Setyati, Dwi; Arimurti, Sattya
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.22645

Abstract

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.
Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.) Azhari, Fitri; Winarsa, Rudju; Siswanto, Siswanto; Muzakhar, Kahar; Utarti, Esti; Sutoyo, Sutoyo; Arimurti, Sattya
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.19034

Abstract

Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbon source for growth. The purpose of this study was to determine the growth pattern and changes in environmental factors, namely the pH value of the probiotic bacteria L. casei FNCC0900 growth medium. The parameters in this study consisted of the highest cell density, generation time and pH value changes in Glucose Yeast Peptone Liquid Media, Porang Boiled Water Media and Porang Flour Liquid Media using the drop plate method which had 4 repeated calculations. Porang Boiled Water Liquid Media has a faster log phase period with a higher cell density than Porang Flour Liquid Media, but the shortest generation time is found in Porang Flour Liquid Media with the highest number of generations. L. casei FNCC0900 bacteria are more able to reduce the pH of Glucose Yeast Peptone Liquid Media compared to porang tuber-based media, so in this case L. casei FNCC0900 can be stated to be able to grow on porang tuber-based media with growth patterns, generation time, cell density and pH value. which varies.