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Pengaruh Perbedaan Salinitas Terhadap Viabilitas Bakteri Pseudomonas spp. Arisandi, Apri; Wardhani, Maulinna Kusumo; Badami, Kaswan; Sopiyanti, Anisa
Rekayasa Vol 10, No 1: April 2017
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (612.678 KB) | DOI: 10.21107/rekayasa.v10i1.3600

Abstract

ABSTRAKKondisi perairan laut yang fluktuatif dan cenderung ekstrim menyebabkan rumput laut mudah terserang penyakit ice-ice. Munculnya bercak putih pada thallus rumput laut Eucheuma cottonii yang terinfeksi penyakit ice-ice diduga disebabkan oleh bakteri patogen yaitu Pseudomonas spp.  Tujuan yang ingin dicapai dalam pelaksanaan penelitian ini adalah untuk mengetahui pengaruh perbedaan salinitas terhadap viabilitas bakteri Pseudomonas spp dan untuk mengetahui pada salinitas berapakah bakteri Pseudomonas spp tidak dapat tumbuh. Bakteri diidentifikasi melalui uji biokimia, dan viabilitas diamati dengan menanam bakteri pada media TSA plate dengan salinitas 30, 32, dan 34 ppt dengan 3 kali pengulangan, dan untuk uji konfirmasi bakteri di tanam pada media TSA miring dengan kadar salinitas 0, 20, 40, 60, 80, dan 100 ppt. Hasil yang diperoleh menunjukan bakteri tumbuh dengan normal pada hampir semua media uji kecuali pada media 100 ppt, hal ini menunjukan bahwa bakteri Pseudomonas spp merupakan bakteri yang bersifat halofilik atau dapat tumbuh baik pada kadar salinitas yang tinggi. Kata Kunci: Eucheuma cottonii, ice-ice, Pseudomonosa spp,Viabilitas.  The Influence of Salinity Difference to Viability of Pseudomonas spp. BacteriaABSTRACTThe conditions of marine waters that fluctuate and tend to extremes can cause seaweed susceptible to ice-ice disease. The appearance of white patches on infected Eucheuma cottonii seaweed thallus is estimated as ice-ice disease which is caused by pathogenic bacteria of Pseudomonas spp  The objectives of this research is to determine the effect of salinity difference to viability of bacteria Pseudomonas spp and to know on what salinity is the bacterium Pseudomonas spp that can’t grow. Bacteria were identified by biochemical tests. Viability was observed by planting bacteria on TSA plate medium with salinity 30 ppt, 32 ppt, and 34 ppt with 3 repetitions, and for bacterial confirmation test in planting on TSA tilting with salinity 0 ppt, 20 ppt, 40 ppt, 60 ppt, 80 ppt, And 100 ppt. The results showed that bacteria grew normally in almost all test media except in 100 ppt media, this showed that Pseudomonas spp bacteria were halophilic bacteria or could grow well at high salinity levels.Keywords: Eucheuma cottonii, ice-ice, Pseudomonosa spp, Viability.
Jumlah Koloni pada Media Kultur Bakteri yang Berasal dari Thallus DAN Perairan Sentra Budidaya Kappaphycus Alvarezii di Sumenep Arisandi, Apri
Rekayasa Vol 9, No 1: April 2016
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.824 KB) | DOI: 10.21107/rekayasa.v9i1.3332

Abstract

Pemberdayaan Industri Perikanan Madura Melalui Pengembangan Budidaya Laut Arisandi, Apri; Rokhmaniati, Siti
Rekayasa Vol 1, No 2: Oktober 2008
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1121.188 KB) | DOI: 10.21107/rekayasa.v1i2.2176

Abstract

Indonesian as marine continent endowed with vast marine resources, in actual facttwo third of Indonesian territory are sea. Merinefisheries, particularl y capturefisheries, is one of the mine effort to make the most of marine resources for supporting economic development in the country. Overfishing then become the mine concern of thegovernment of Indonesian in marine resource utilization. The data reflects that the exploitation of the resources is approaching the Maximum Sustainable Yield (MSY), especially of the small pelagic fish. Considering theproblems of overexploitation, it is therefore significant todevelop marinefish farming (marine aquaculture or mariculture) as an alternative. Theactivities in mariculture is mainly farming fishes and plant of high value on export market such as Grouper (ikan kerapu) and Seaweed (Rumput laut), but the development of tnariculture in Indonesia is still not as expected. The investor still hesitate to. enter this business because they still feel unsecure due to lack of law and regulations concerning the use of sea waters for mariculture activities. Theconcept of "Mariculture Idustries" is raised togive a solution to this problems. The idea is that the local authority (Pemerintah Kabupaten) enacted a decree that a certain area of sea water isprotected from other activities exceptfor mariculture. By assigning a certain.regulating body, the area isprotected and maintained professionall y. Theregulating body can invite investors toenter the bisnis and alsofacilitate theminrunning their mariculture activities. This model of mariculture industries will try in Madura.
TOKSISITAS HORMON 17 α METILTESTOSTERON TERHADAP ASPEK REPRODUKSI UDANG GALAH Arisandi, Apri
Rekayasa Vol 6, No 1: April 2013
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.965 KB) | DOI: 10.21107/rekayasa.v6i1.2101

Abstract

Udang Galah (Macrobrachium rosenbergii) betina diperlakukan tanpa hormon dan dengan penyuntikan hormon Methyltestosterone (MT) dengan kadar 15 µg/g (1 level) dan dipelihara/diamati selama 60 hari. Setelah pelakuan tesebut ternyata memberikan pengaruh nyata terhadap beberapa aspek reproduksi udang galah, yaitu derajat pengeraman, derajat penetasan, ukuran telur, derajat hidup dan kecepatan tumbuh larva. Seperti pada kontrol buatan terhadap aspek reproduksi pada budidaya udang galah dengan hormon tersebut tidak memungkinkan digunakan pada pengelolaan budidaya yang lain. Studi ini memungkinkan sebagai inspirasi untuk penelitian yang sama pada budidaya udang-udang air tawar besar di Indonesia.