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All Journal Jurnal Penelitian Hasil Hutan Jurnal Riset Kimia
Yosi Aristiawan
Organic Metrology Research Group, Research Center for Chemistry, Indonesian Institute of Sciences, Komplek Puspiptek, Serpong, Tangerang, Banten 15314
Agriculture, Biological Sciences & Forestry Chemistry Decision Sciences, Operations Research & Management

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SAKARIFIKASI SERAT TANDAN KOSONG DAN PELEPAH KELAPA SAWIT SETELAH PRETREATMENT MENGGUNAKAN KULTUR CAMPURAN JAMUR PELAPUK PUTIH Phanerochaete chrysosporium dan Trametes versicolor Hermiati, Euis; Risanto, Lucky; Anita, Sita Heris; Aristiawan, Yosi; Hanafi, Ahmad; Abimanyu, Haznan
Jurnal Penelitian Hasil Hutan Vol 32, No 2 (2014): Jurnal Penelitian Hasil Hutan
Publisher : Pusat Litbang Keteknikan Kehutanan dan Pengolahan Hasil Hutan

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Abstract

Penggunaan kultur campuran jamur pelapuk putih pada proses pretreatment bahan lignoselulosa belum banyak diteliti. Penelitian ini bertujuan untuk mempelajari pengaruh penggunaan kultur  Phanerochaete chrysosporium dan Trametes versicolor pada proses pretreatment serat tandan kosong dan pelepah kelapa sawit terhadap hasil sakarifikasinya. Inokulum P. Chrysosporium dan T. versicolor dituangkan ke dalam sampel substrat serat tandan kosong dan pelepah kelapa sawit (40-60 mesh) yang telah disterilkan, masing-masing sebanyak 5% (w/v), sehingga total inokulum yang ditambahkan ke dalam kedua macam substrat masing-masing 10% (w/v). Sampel diinkubasikan pada suhu ±27°C selama 4 minggu. Sebagian dari contoh diambil dan lalu diperiksa setelah masa inkubasi 1, 2, 3, dan 4 minggu. Sakarifikasi dilakukan menggunakan enzim selulase sebanyak 20 FPU per g biomassa dan β-glukosidase dalam shaking waterbath pada suhu 50°C selama 48 jam. Analisis gula pereduksi, glukosa dan xilosa dilakukan terhadap hasil sakarifikasi. Rendemen gula pereduksi, konsentrasi glukosa dan xilosa tertinggi dari tandan kosong kelapa sawit. diperoleh dari sakarifikasi serat dengan pretreatment selama 4 minggu, yaitu masing-masing 13,08%, 0,86 mg/g dan 0,13 mg/g, sedangkan rendemen gula pereduksi, konsentrasi glukosa dan xilosa tertinggi dari pelepah kelapa sawit didapatkan dari sakarifikasi substrat dengan pretreatment selama 2 minggu, yaitu masing-masing 8,98%, 0,92 mg/g dan 0,23 mg/g.
Validation and Uncertainty Evaluation of an LC-DAD Method for Simultaneous Quantification of Benzoic Acid, Methylparaben, and N-Butylparaben in Soy Sauce Aristiawan, Yosi; Ramadhaningtyas, Dillani Putri; Styarini, Dyah
Jurnal Riset Kimia Vol 13, No 1 (2022): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v13i1.432

Abstract

Chemical food preservation is the common strategy used by human to preserve the natural properties and to increase the shelf life of food. Although preservatives are useful to keep the food fresh and to stop the bacterial growth, there are certain preservatives that are harmful if taken in more than the prescribed limits. Some of the typical used—benzoic acid, methylparaben, and n-butylparaben—were employed in this work with the aim of establishing a simultaneous liquid chromatography (LC) method for detecting each in soy sauce matrices. Liquid and C18 solid phase extraction were performed in this procedure prior to LC using Diode Array Detector analysis. In gradient elution of a format buffer (pH 4.4) and acidified acetonitrile, the target components were successfully separated. Calibration curve ranged from 0.61–140 mg/kg linearly while the limit of quantification for benzoic acid, methylparaben, and n-butylparaben were 0.41, 0.10, and 0.11 mg/kg, respectively. The intermediate precision and recovery were in the range between 0.15-1.89% and 100.5-103.3%, respectively. The expanded uncertainty (k=2) in sample measurement was estimated at 3.4-6.5%. The offered method was conformed to the validation acceptance criteria and can be applied as a routine method in the laboratory at ppm level.
Co-Authors Ahmad Hanafi Dyah Styarini Euis HERMIATI Haznan Abimanyu Lucky Risanto Ramadhaningtyas, Dillani Putri Sita Heris Anita