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Profile of Interleukin-4 and Interferon-γ of Balb/c Mice after Salivary Gland Extract of Anopheles sundaicus s.l. Vaccination and Infected by Plasmodium berghei-ANKA Machrus, Ali; Adrial, Adrial; Armiyanti, Yunita; Wiyono, Hidayat Teguh; Senjarini, Kartika
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.451 KB) | DOI: 10.19184/jid.v15i2.532

Abstract

Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite,  the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of  IFN-γ and decreasing level of IL-4 in mice model  of malaria after vaccination with  salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this  mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei.   Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV
Description of Leukocytes Differential Count in Coffee Plantation Workers Silo Subdistrict that Infected by Soil-transmitted Helminths Cahyani, Desi Dwi; Armiyanti, Yunita; Komariyah, Cicih; Hermansyah, Bagus; Nurdian, Yudha
Journal of Agromedicine and Medical Sciences Vol 6 No 1 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i1.9620

Abstract

Helminthiasis caused by soil transmitted helminths (STH) are still a serious health problem in the world and in Indonesia. Indonesia has a prevalence that varies between 2.5%-62%. This infection can cause blood disorders such as leukocytosis, eosinophilia, and changes in hemoglobin levels. The purpose of this study was to describe the leukocyte count of coffee plantation workers that infected by STH. This research was an observational descriptive study, using a cross sectional design and was conducted at the coffee plantation in Silo subdistrict. Stool examination was conducted by the concentration method (sedimentation and flotation) to determine the presence of STH infection, while for leukocyte count, we used the differential count method. The results of examination on 101 feces samples showed 26.7% (27/101) of workers were positively infected with STH, with details of the STH type of hookworm by 92.6% (25/27), and the remaining were double infections by Ascaris lumbricoides and hookworm as much as 7.4 % (2/27). Examination of leukocyte count showed, 17 workers with hookworm infection had abnormal leukocyte count i.e eosinophilia and neutrophilia, while 8 workers were normal. All workers with double infection (2 workers) had abnormal leukocyte count i.e eosinophilia and neutrophilia. These results can be influenced by the chronicity of the infection or the intensity of the infection. Keywords: Chronic Kidney Disease, sodium, re-use hemodialyzer
Association between Personal Hygiene and Incidence of Soil-Transmitted Helminthiasis among Workers at Widodaren Plantation in Jember Regency Rahmawati, Zulaikha Rizqina; Hermansyah, Bagus; Efendi, Erfan; Armiyanti, Yunita; Nurdian, Yudha; Utami, Wiwien Sugih
Journal of Agromedicine and Medical Sciences Vol 6 No 1 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i1.9593

Abstract

Soil-Transmitted Helmithiasis is an infection caused by intestinal nematode worms which in the process of transmission requires soil as media to fullfil its life cycle from non-infective forms to infective forms. The number of infections is influenced by personal hygiene. Personal hygiene consists of the habit of washing hands, cutting nails, eating, defecating and ownership of latrines, and the use of personal protective equipment. This study used a cross sectional research design conducted at Widodaren Plantation with 68 people of samples. Stool examination was determined by kato-katz method, sedimentation, and floatation to detect the presence of Soil-Transmitted Helminth (STH) eggs, while personal hygiene was assessed using questionnaires. Questionnaire and stool examination datas were processed using SPSS data analysis with chi-square method. The results of this study found 26 respondents who were positive for soil-trasmitted helminthiasis infection. The most frequent worm species obtained in this study were Ascaris lumbricoides (57.7%), followed by multiple infections between Hookworm and A. lumbricoides (21.3%), and Hookworm (19.2%). In the chi-square analysis the results were significant between personal hygiene and soil-transmitted helminthiasis with p value of 0,000 (p <0.05). So, it can be concluded that a bad personal hygiene factor has a significant relationship to the incidence of Soil-Transmitted Helminthiasis at Widodaren Plantation. Keywords: Personal hygiene, STH, plantation
PENGARUH PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus) TERHADAP STRES OKSIDATIF SEL ENDOTEL YANG DIPAPAR DENGAN SERUM PENDERITA MALARIA FALCIPARUM DAN NETROFIL INDIVIDU SEHAT Armiyanti, Yunita; Fitri, Loeki Enggar; Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 23, No 1 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1699.473 KB) | DOI: 10.21776/ub.jkb.2007.023.01.2

Abstract

Complication in Plasmodium falciparum malaria is associated with endothelial damage and overproductionof free radicals (oxidative stress) by activated neutrophils and endothelial cells. Red fruit (Pandanus conoideus) oil  has a very high antioxidant content, therefore it may neutralize free radicals and prevent endothelial damage. The aim of this research is to prove that red fruit (Pandanus conoideus) oil reduce Reactive Oxygen Intermediate (ROI) production  of endothelial cells exposed to severe malaria patient serum and neutrophils from healthy donor. Endothelial cells from human umbilical veins were coincubated with serum from severe malaria patient and with neutrophils from healthy donor  (positive control group). HUVEC’s normal was used as negative control,  where as  the experimental groups were given with red fruit oil in different concentrations (2.8%, 5.7% dan 11.3% ). The Reactive Oxygen Intermediate (ROI) production of  endothelial cells was semiquantitatively measured by using NBT-reduction assay and the score of ROI was counted. The results were statistically analyzed with ANNOVA (p<0.01). The rate of ROI production of endothelial cells was markedly increased after incubation with patient serum and neutrophils.
Imunogenic Protein of Salivary Gland from Anopheles sundaicus Armiyanti, Yunita; Nuryady, Moh Mirza; Utomo, Sugeng Setyo; Sardjono, Teguh Wahju; Fitri, Loeki Enggar; Senjarini, Kartika
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Malaria is still a major problem for developing countries, including Indonesia. One approach to overcome this disease is prevention by vaccination. However, there is still no effective malaria vaccine that is applicable. The ideal malaria vaccine is a combination vaccine that can prevent the pre-erythrocytic cycle, the erythrocytic cycle and transmission process. Salivary vector-based vaccine has the potential to be developed as a malaria vaccine because it can prevent transmission process and also decrease the morbidity of the disease. Saliva from Anopheles contains vasomodulator and immunomodulatory components, that are required in the blood feeding process, but in the same time it could enhance the transmission of the malaria parasite. If the component in the salivary vector can increase pathogen infection, then vaccinating the host with its anti-substances can control the transmission of pathogens (Transmision Blocking Vaccine). Anopheles sundaicus is an important vector of malaria in coastal areas of Java, Bali, Sumatra, Kalimantan and West Nusa Tenggara islands. Repeated exposures of Salivary Gland Extract (SGE) from this vector have been proven to be able to decrease parasitemic rates in mouse model for malaria in our study. The objective of this research is to determine and localize the immunogenic protein from SGE of An. sundaicus as the first step for the characterization of its immunomodulatory component. Mosquito salivary gland protein profile of An.sundaicus was determine by SDS-PAGE. Determination of salivary glands immunogenic proteins was conducted by Western Blotting with IgG from people living from endemic area as primary antibody. Out of 15 bands appeared in SDS PAGE ranging from 24 kD to 138 kD, only two protein bands with  molecular weights of 68 and 37 kDa were the most immunogenic. Those immunogenic proteins were consistent recognized by pooled serum of people as well as by individual response. Keywords: malaria, saliva, vector, immunogenic protein, vaccine
EOSINOPHILIA AS A PREDICTOR MORBIDITY OF SOIL- TRANSMITTED HELMINTHIASES AMONG WIDODAREN PLANTATION WORKERS IN JEMBER Primadana, Aditya; Nurdian, Yudha; Agustina, Dini; Hermansyah, Bagus; Armiyanti, Yunita
Journal of Vocational Health Studies Vol. 3 No. 2 (2019): November 2019 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.887 KB) | DOI: 10.20473/jvhs.V3.I2.2019.47-52

Abstract

Background: Helminthiasis is a neglected tropical disease and still remain a health problem in tropical and sub-tropical countries, including Indonesia. According to WHO, approximately 1,5 billion people or 24% of the world’s population, are infected with Soil-Transmitted Helminth (STH). Morbidity is directly related to the intensity of STH infestation. Some of the studies shown a significant correlation between eosinophilia and helminthiasis. Eosinophilia on the blood smear examination may be used as a marker of STH infestation. Purpose: to determine the correlation between Soil-Transmitted Helminthiases and eosinophilia as a predictor of morbidity of STH infestation among Widodaren Plantation Workers in Jember. Methods: This observational analytical study use the Kato-Katz method to determine infestation of STH and its intensity measured by egg count per gram stool (EPG). Blood smear examination stained with Giemsa conduct to determine eosinophil count. Results: Prevalence of STH infestation was 22,7% (15/66). Ascaris lumbricoides infection was 13,6% (9/66) and Hookworm infection was 4,5% (3/66). There is mixed infection between A. lumbricoides and Hookworm, the prevalence was 4,5%% (3/66). All of the infection intensity was light. The prevalence of eosinophilia was 27,2% (18/66). There was a significant correlation between STH infection and eosinophilia (P=0,000) but, there was a very weak correlation between the intensity of STH infection and eosinophilia (r=0,281; p=0,258). Conclusion: The prevalence of STH infection among Widodaren Plantation Workers is 22,7% and predominated by A. lumbriocides. There is a positive correlation between intensity of STH infection and eosinophilia as predictor morbidity of STH infection with very weak power.
Hubungan perilaku hidup bersih dan sehat dengan kejadian infestasi Soil–transmitted Helminths pada pekerja perkebunan kopi Sumber Wadung Kabupaten Jember Al-Muzaky, Ahmad Hasbi; Hermansyah, Bagus; Suswati, Enny; Armiyanti, Yunita; Nurdian, Yudha
JURNAL KEDOKTERAN DAN KESEHATAN Vol 6, No 1 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.379 KB) | DOI: 10.32539/JKK.v6i1.7233

Abstract

Infestasi Soil - Transmitted Helminths (STH) merupakan masalah kesehatan yang masih sering ditemukan di negara beriklim tropis, salah satunya di Indonesia. Hal ini dikarenakan perkembangan STH membutuhkan kondisi lingkungan yang hangat dan lembab, serta  memerlukan media tanah untuk menjadi fase infektif yang disebut soil - transmitted helminth.Kondisi ini dapat ditemukan di lingkungan perkebunan yang sangat menguntungkan bagi perkembangan STH. Hal ini menyebabkan para pekerja perkebunan sangat beresiko terinfestasi oleh STH. Tujuan penelitian ini adalah mengetahui adanya hubungan perilaku hidup bersih dan sehat (PHBS) dengan kejadian infestasi STH pada pekerja perkebunan kopi. Jenis penelitian ini adalah penelitian analisis deskriptif dengan desain cross-sectional yang dilaksanakan pada bulan Desember 2018 hingga Januari 2019. Perkebunan yang dipilih adalah Perkebunan Kopi Sumber Wadung di Kabupaten Jember dengan jumlah sampel sebanyak 100 orang responden dan diberikan kuisioer PHBS. Pemeriksaan infestasi STH dilakukan dengan dua metode yaitu flotasi dan sedimentasi. Dari 100 responden, 28 pekerja (28%) terinfestasi oleh STH. Kemudian dilakukan uji analisis chi-square antara PHBS dengan kejadian infestasi STH dan didapatkan adanya hubungan antara PHBS dengan kejadian infestasi STH pada pekerja (<0,05).
The Correlation Between The Use of Personal Protective Equipment (PPE) and Soil-Transmitted Helminths Infection in the Workers of Kaliputih Plantation Jember Regency Baidowi, Ivan Iqbal; Armiyanti, Yunita; Febianti, Zahrah; Nurdian, Yudha; Hermansyah, Bagus
Journal of Agromedicine and Medical Sciences Vol 5 No 2 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i2.9625

Abstract

This study aimed to investigate the correlation between the use of Personal Protective Equipment (PPE) and the status of Soil-Transmitted Helminths infection in the workers of Kaliputih plantation in Jember Regency. The research method applied was Analytic observational with a Cross-sectional approach. The population, as well as the sample, were all workers of Kaliputih Plantation, Sumber Bulus Village, Ledokombo District, Jember Regency, by the total of 63 people. The sampling technique was the Total sampling. Laboratory analysis on faecal samples of respondents were conducted at the Parasitology Laboratory, Faculty of Medicine, University of Jember. Furthermore, the research data were analyzed using Chi-Square analysis or Fisher’s Exact Test. The results demonstrated that the prevalence of STH infection in Kaliputih Plantation workers was 25%. This figure was supported by the good awareness of the use of Personal Protective Equipment (PPE) of workers (91.67%). In addition, the Soil-Transmitted Helminths species that infested the plantation workers were Ascaris lumbricoides, found in 6 people (16.67%), and Hookworm, found in 3 people (8.33%). Finally, the result of Chi-square analysis showed a significance value of <0.05. Therefore, it can be concluded that the use of PPE in Kaliputih plantation workers had a significant correlation with the STH infection status. Keywords: Correlation, PPE, Soil-Transmitted Helminths Infection
Humoral Response to Anopheles maculatus and Anopheles sundaicus Salivary Gland Proteins Armiyanti, Yunita; widodo, Widodo; Fitri, Loeki Enggar; Sardjono, Teguh Wahju
Journal of Agromedicine and Medical Sciences Vol 3 No 2 (2017)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v3i2.5071

Abstract

The salivary gland of female Anopheles mosquito has important role in malaria transmission by salivary proteins that enhancing Plasmodium transmission. The mosquito saliva contains vasomodulatory and immunomodulatory components that inhibit the physiological response of the host. Therefore, the pathogen infects the host without any resistance. The salivary proteins also induce the production of antibody IgG in the host after exposed by the Anopheles mosquito bites repeatedly. This study aims to measured the antibody response of inhabitants living in malaria endemic areas (Kalirejo viilages, Kokap, Kulonprogo) to salivary gland proteins of An. maculatus and An.sundaicus. The level of antibody response was measured by ELISA and analyzed with T test or Anova test for normal distribution of data and Mann Whitney test or Kruskal Wallis test for the data were not normally distributed. The result showed that the level of anti-salivary gland homogenate IgG from sera of people living in malaria endemic area was significantly higher than people living in non-malaria endemic area and negative control (p<0.05). Sera from inhabitants living in Kalirejo village showed that the level of antibody response to both salivary gland homogenate of An.sundaicus and An.maculatus were not significantly different (p<0.05). It was concluded that exposure to An.maculatus and An.sundaicus bites repeatedly could trigger the production of anti-salivary gland proteins IgG antibodies that recognized antigenic proteins from the salivary glands.
The Correlation of Risk Factors to the incidence of Pediculosis capitis on Students in Pondok Pesantren Miftahul Ulum, Jember Lukman, Nihayah; Armiyanti, Yunita; Agustina, Dini
Journal of Agromedicine and Medical Sciences Vol 4 No 2 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i2.6488

Abstract

ABSTRACT Pediculosis capitis is a scalp disease caused by obligate ectoparasite (mite or lice) infection of Pediculus humanus var. capitis. This disease is a contagious disease that influenced by environment and behavior, so it becomes a dominant public health problem in densely populated environment like in boarding school. Some risk factors that cause Pediculosis capitis are; sex, educational level, hair wash frequency, use of comb or hair accessories together, use of bedding or bed together, hair length, and hair type. This study aims to determine the most dominant incidence, analyze the risk factors, and know the biggest risk factors to the incidence of Pediculosis capitis in santri at Pondok Pesantren Miftahul Ulum, Jember. The type of research that used was observational analytics with case control design conducted on 287 samples of SMP and SMA Miftahul Ulum students who fulfilled the inclusion and exclusion criterias. From this research, the proportion of Pediculosis capitis incidence was 214 respondents (74,6%), and then analyzed by Chi-Square test, it was found that the variables had significant relationship (p <0,05) to Pediculosis capitis occurrence, among others; sex, hair wash frequency, use of comb or hair accessories together, use of bedding or bed together, hair length and hair type. The result of logistic regression test shows that sex was the most biggest variable (p value = 0,000). Keywords: Pediculosis capitis, risk factors, boarding school