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All Journal Media Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Ilmiah Teknologi Pertanian Agrotechno Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences

I Gusti Agung Arta Putra

Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 233946

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Karakteristik Lokus Mikrosatelit D8S1100 pada Populasi Monyet Ekor Panjang di Bukit Gumang, Karangasem, Bali. Rumba, Jefri Ndawa; Wandia, I Nengah; Arta Putra, I Gusti Agung
Indonesia Medicus Veterinus Vol 2 (1) 2013
Publisher : Faculty of Veterinary Medicine, Udayana University

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Pengungkapan berbagai karakteristik genetik populasi memiliki nilai yang strategis. Selain merupakan khasanah informasi ilmiah, karakteristik tersebut dapat digunakan untuk memprediksi viabilitas masa depan suatu populasi, dan bahan pertimbangan untuk usaha-usaha konservasi. Mikrosatelit merupakan marka/penanda molekuler pilihan untuk studi pilihan genetik populasi. Mikrosatelit adalah segmen langsung dari materi genetik (DNA), sehingga penggunaannya sebagai marka molekuler akan lebih mencerminkan karakteristik genetik populasi. Penelitian ini dilakukan untuk mengkarakterisasi lokus mikrosatelit D8S1100 dan mendapatkan informasi awal mengenai status poplimorfisme sebelum dipilih sebagai marka molekuler dalam studi genetik populasi. Variabel yang diamati adalah jumlah dan jenis alel, frekuensi, dan heterozigositas. Sembilan belas sampel darah monyet dikoleksi dari di Bukit Gumang, Karangasem, Bali, sebagai sumber DNA. DNA total diekstraksi menggunakan QIAmp DNA Blood Mini Kit. Lokus mikrosatelit D8S1100 di amplifikasi dengan teknik polymerase chain reaction (PCR), sebanyak 30 siklus dengan suhu annealing 540C. Selanjutnya, alel dipisahkan dengan elektroforesis pada gel poliakrilamid 7% dan dimunculkan dengan pewarnaan perak. Hasil penelitian menunjukkan bahwa hanya ditemukan satu jenis alel pada lokus D8S1100 dalam populasi monyet ekor panjang di Bukit Gumang dengan panjang alel 196 pasang basa. Dapat disimpulkan bahwa lokus D8S1100 pada populasi monyet ekor panjang di Bukit Gumang bersifat monomorfik. Hasil penelitian mengindikasikan bahwa lokus mikrosatelit D8S1100 kurang baik digunakan sebagai marka molekuler untuk mengkaji variasi genetik populasi monyet ekor panjang di Bukit Gumang.

CM-10 Histopathological, Serological and Molecular Confirmation of APMV- 1 Infection I Gusti Agung Arta Putra; Anak Agung Ayu Mirah Adi
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Histopathological, Serological and Molecular Confirmation of APMV- 1 Infection

Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed Macaque Populations in Bali Island, Indonesia I Nengah Wandia; I Gede Soma; I Gusti Agung Arta Putra
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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The long tailed macaques inhabit throughout Bali Island, however, recently they have been fragmented into many smaller local populations. In spite of that, there are just few reports that describe the mode of fragmentation and the genetic structure of the population. This research aimed to explore the polymorphism of microsatellite loci on Y chromosome in long tailed macaque populations in Bali Island using three human microsatellite primers, namely DYS390, DYS391, and DYS393. A total of 99 blood samples were collected from male long tailed macaques originated from 8 populations (23 from Pulaki, 11 from Bedugul, 13 from Mekori, 8 from Sangeh, 6 from Uluwatu, 11 from Alas Kedaton, 11 from Ubud, and 16 from Bukit Gumang). Total DNA was extracted using QIAamp® Blood Mini Kit. The polymorphism of the microsatellite loci was detected using PCR technique, and allelic variations were separated through 7% poly acrylamide gel electrophoresis. The result of the research showed that DYS390 and DYS393 loci were monomorphic (each had only one allele). The DYS391 locus had two alleles, therefore, this locus was polymorphic. It could be suggested that DYS391 locus could be used to study genetic variation in male long tailed macaque population in Bali Island.

Genetic Structure in Long Tailed Macaque Populations in The Region of East Java: Diversity of Mithochondrial DNA in Alas Purwo and Baluran Population I Nengah Wandia; I Gusti Agung Arta Putra; I Gede Soma
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

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Long tailed macaque (Macaca fascicularis) has distributed throughout the regions in Indonesia, however,they, now, live separately to many local populations within an island. This research aimed to explore thegenetic diversity within and between populations of long tailed macaque in the Region of East Java. Thegenetic diversity was examined by molecular marker of d-loop region mtDNA using a pair of specificprimers. A total of 29 blood samples (15 samples from Alas Purwo population and 14 samples fromBaluran population) was extracted to find out a total DNA. The d-loop region mtDNA was amplifiedthrough PCR tecnique. The PCR products were sequenced using dideoxynucleotide chain terminationmethod at Macogen Inc. Korea. The research found 6 haplotypes, in which 3 haplotypes were found in eachpopulation. Haplotype and nucleotide diversities varied from 0.257 to 0.275 and 0.0012 to 0.0025repectively. Genetic differentiation between populatioon was F=44.1%. Phylogenetic reconstructionshowed that all haplotypes constructed two different groups (haplogroups) and there was no sharedhaplotype betwen populations. It can be concluded that both populations (Alas Purwo and Baluran) areuique and have low genetic diversities.

TEKNIK DAN MANAJEMEN PRODUKSI BIBIT BABI DI BANJAR DAUH UMA, DESA PENGOTAN, KABUPATEN BANGLI NLG SUMARDANI; IGA ARTA PUTRA; IW SUBERATA; IG SURANJAYA; DK HARYA PUTRA; WS YUPARDHI
Buletin Udayana Mengabdi Vol 14 No 1 (2015): April 2015
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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The short course of management of animal husbandry and using Artificial Insemination (AI) on swine wasconducted on September 6th 2014 at Dauh Uma Pengotan Village, Regency of Bangli. There were 15 farmers whomerged into group as participants of this activity. The aimed of this activity was to increase litter size of swinewith AI program in breeding management. The used method of activity was giving counseling about breedingand health management of animal husbandry in breeding farm, AI program, and then practiced inseminate onswine. Based on the process of short course it was concluded that, the response of participants on AI was veryenthusiastic in joining the activity.Keywords : animal husbandry, breeding, artificial insemination, litter size, swine.

VASEKTOMI DAN PEMOTONGAN TARING PADA MONYET EKOR PANJANG (Macaca fascicularis) DI KAWASAN WISATA PURA BATU PAGEH, DESA UNGASAN BADUNG I Nengah Wandia; I Gede Soma; I Ketut Suatha; I GA. Arta Putra; Sri Kayati Widyastuti; Aida Louise Tenden Rompis
Buletin Udayana Mengabdi Vol 11 No 2 (2012): Volume 11 No.2 – September 2012
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Population growth and aggressiveness are important factors on macaque populations that their habitats are used as tourist destinations. Controlling those two factors may decrease the negative impact of the macaque population both to the environment and the internal circumstance of the population. This Community Service was packed as a vasectomy and canine cutting services on the adult male of long tailed macaques occupying a tourist destination of Pura Batu Pageh, Ungasan Village, Badung Regency. Vasectomy is a surgical division of all or part of vas deferens to induce sterility. Surgical resection of part of vas deferens used a midline slicing method. In this program, one selectively adult male was successfully vasectomized and the four canines were also cut successfully. Later inspection showed that there was no any problem with the individual that formerly subjected to medical treatments and even the wounds went to be healing completely.

Karakteristik Molekuler Virus Avian Orthoavulavirus 1 Genotipe VII yang Diisolasi dari Tabanan Bali (MOLECULAR CHARACTERISTIC OF AVIAN ORTHOAVULAVIRUS 1 GENOTYPE VII ISOLATED FROM TABANAN BALI) Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Nengah Wandia; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Anak Agung Keswari Krisnandika; Anak Agung Oka Wijaya
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Newcastle disease (ND) is a very harmful avian disease, endemic in Indonesia and various parts of the world. The causative agent is ND virus or Avian orthoavulavirus 1 (AOAV-1). This virus is an RNA virus with wide genetic variation. Based on the genome length, it can be classified into AOAV-1 Class I and II. Class I are generally avirulent whereas Class II are consist of both virulent and avirulent viruses, currently there are 18 genotypes of the class II. To find out the molecular characteristics of AOAV-1 currently circulating in the field, isolation and identification of viruses from laying hens that was suspected ND from Tabanan Bali in 2017, was performed. The isolated viruses hereafter named as Tabanan1/ARP / 2017. A one-step RT-PCR reaction was carried out to amplify NP, F and HN gene fragments from the virus using three specific pairs of AOAV-1 primers. The obtained nucleotide sequences are then used in phylogenetic analysis. For phylogenetic analysis several strains of AOAV-1 from class II representing genotype I-VII as well as one strain from Class I were accessed from GenBank. From the analysis of the F gene nucleotide sequences, it was found that Tabanan 1 / ARP / 2017 is a genotype VII virus with an amino acid sequence at the F protein cleavage site is 112 R-R-Q-K-R-F117, a typical virulent strain. Phylogenetic analysis using nucleotide sequences NP and HN genes also positioned this isolate in genotype VII. At the nucleotide level, genetic distance with virulent isolates that was isolated in 2007 and 2010 were 8.26% and 1.08% while at the amino acid level were 5.26% and 0.64%. There were found mutations in amino acids at positions 107 and 108 of F protein.

Variasi Molekuler Gen Reseptor Melanokortin-4 pada Monyet Ekor Panjang I Gusti Agung Arta Putra; Dondin Sajuthi; Dedy Duryadi Solihin; Raden Roro Dyah Perwitasari
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Melanocortin-4 receptor (MC4R) is one of G protein-coupled receptors that plays an important role inregulation of energy homeostasis. MC4R mutations constitute the most common cause of human obesity.The study was conducted in order to investigate the variation of MC4R gene in Macaca fascicularis and itsassociation with obesity as a model of human obesity. Forty eight adult male macaques from Bali (Ubudand Uluwatu), East Java (Alas Purwo and Baluran), and Sumatera island (Palembang) were used in thisresearch. The animals had been anaesthetized using ketamine (10 mg/kg body weight) and xylazine (2mg/kg body weight) before collecting blood samples and phenotypic data (weight, crown rump length. Bloodsamples were used as source of DNA. To determine MC4R variation, coding region of this gene wasamplified and sequenced. The results showed that 20 variations sites were identified and 13 of them werenon-synonymous. Among the non-synonymous mutations, five mutations were only found in obese macaque;two mutations were found both in obese and non-obese macaque; and six mutations were only found in nonobesemacaque .

Ekstrak Kulit Buah Manggis (Garcinia mangostana Linn) Mampu Meminimalkan Efek Imunosupresif Monosodium Glutamate (EFFECTIVENESS OF MANGOSTEEN PEEL EXTRACT (GARCINIA MANGOSTANA LINN) IN MINIMIZING THE IMMUNOSUPPRESSIVE EFFECT OF MONOSODIUM GLUTAMATE) Anak Agung Bagus Bramardipa; Anak Agung Ayu Mirah Adi; I Gusti Agung Arta Putra
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Monosodium glutamate (MSG) is commonly used as a food additive or flavoring worldwide, unfortunately MSG is reported to have a toxic effect to the immune system, that can lead to an immunosuppressive conditions. One of immunosuppressive signs in chickens is atrophy of bursa Fabricius (BF). Mangosteen peel extract contains active substances and other components that can act as an immunomodulator. The aim of this study was to evaluate the immunosuppressive effects of MSG and the effectiveness of mangosteen peel extract in minimizing the immunosuppressive effects of the MSG. This study used a completely randomized design with four treatments namely A, B, C and K consisted four chickens each. The chickens in treatment A, B, C and K were given feed containing MSG 20% W/W and mineral water for drinking, commercial feed and mineral water containing 2% W/V mangosteen peel extract, MSG 20% W/W and mineral water containing mangosteen peel extract 2% W/V and commercial feed and drinking water without mixture. (control), respectively. Based on the results of this study, it was found that the administration of feed containing 20% MSG could result in bF atrophy with a very low bF index (p <0.01) if compared with B, C, and K-treatment. Prominet histopathological (HP) features of bF of chickens under treatment A are follicular atrophy and interfollicular edema. Treatment with feed containing 20% MSG along with the administration of drinking water containing 2% mangosteen peel extract keeps on maintaining the bF under normal condition even microscopically was found macrophages proliferation inside the lymphoid follicle. Based on clinical symptoms, index of bF and histopathological features, it can be concluded that MSG has immunosuppressive effects on chicks and mangosteen peel extract in drinking water can minimize this effect.

Pembiusan Monyet Ekor Panjang (Macaca fascicularis) dengan Campuran Ketamine dan Xylazine (THE ANAESTHETIZATION OF LONG TAILED MACAQUE (MACACA FASCICULARIS) BY INJECTING THE COMBINATION OF KETAMINE AND XYLAZINE) I Nyoman Suartha; I Nengah Wandia; I Gusti Agung Arta Putra; I Gede Soma; I Gusti Ngurah Sudisma
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Co-Authors Aida Lousie Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Bagus Bramardipa Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Bayu Setiabudi Dewi Ayu Warmadewi Dondin Sajuthi DWI SURYANTO DYAH PERWITASARI -FARAJALLAH I Dewa Ketut Harya Putra I G. Lanang Oka Cakra I Gede Soma I Gusti Ngurah Sudisma I Ketut Eli Supartika I Ketut Puja I Ketut Suatha I Made Kardena i Nengah Wandia I Nyoman Ardika I NYOMAN MANTIK ASTAWA I Nyoman Suartha I WAYAN SUBERATA Ida Ayu Putri Utami Ida Bagus Oka Winaya Jefri Ndawa Rumba Marissa Divia Dayanti Muh. Sofyan Muna Yuniarti Ni Luh Gde Sumardani Nyoman Sadra Dharmawan Palagan Senopati Sewoyo Siswanto - Siswanto Sofyan, Muh. Sri Anggreni Lindawati Sri Kayati Widyastuti Suranjaya I .Gd TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Wayan Sayang Yupardhi

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