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Genetic Variation Analysis of EMS-Induced Chili Pepper (Capsicum frutescens L.) Mutants Using SSR Markers Dwinianti, Edia Fitri; Mastuti, Retno; Arumingtyas, Estri Laras
Journal of Tropical Life Science Vol 9, No 3 (2019)
Publisher : Journal of Tropical Life Science

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Abstract

Mutation induction with chemical mutagen ethyl methane sulfonate (EMS) capable of producing genetic variation in plants. The purpose of this research was to evaluate genetic variation of EMS-induced chili pepper mutants using SSR molecular marker. Various concentration of EMS (0.01%; 0.02%; 0.04%) were applied to chili pepper seeds of Genotypes 2, 7, and 11. The genomic DNA was isolated from mutants and control plants then used for PCR analysis using three SSR primers namely CA26, CA52 and CA96. The three SSR primers were able to detect genetic variation in the three genotypes of EMS-induced chili pepper mutants and control plants. A total of 18 alleles have been identified in all three SSR loci with PIC values ranging from 0.792 to 0.893. The three genotypes of chili pepper plants had different sensitivity to EMS mutation induction. Genotype 11 plants showed higher sensitivity to EMS treatment than genotypes 2 and 7 based on the amount of genomic changes. Dendrogram constructed based on genetic similarity values distributed the mutants and control plants into three main groups with similarity coefficients ranging from 0.34 to 0.90. Similar genotype either control plant or mutants, especially Genotypes 2 and 7 were group into similar group. However, for the Genotype 11 plants, large genome changes cause mutant plants G11K1, G11K2, G11K3 had a low genetic similarity to their control plants, so the mutants were separated in a different group from the control plant. Those indicate that EMS mutation induction capable of increasing genetic variation in chili pepper plants based on SSR molecular marker.
Effect of Bulbils Position on Leaf Branches to Plant Growth Responses and Corms Quality of Amorphophallus muelleri Blume Mastuti, Retno; Harijati, Nunung; Arumingtyas, Estri Laras; Widoretno, Wahyu
The Journal of Experimental Life Science Vol 8, No 1 (2018)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1140.975 KB) | DOI: 10.21776/ub.jels.2018.008.01.01

Abstract

Bulbil (corm leaf) is one material source of vegetative propagation in Amorphophallus muelleri Blume. Based on the position in branches there are two types of bulbil, middle and edge bulbils, which are different in shape and size. It has been reported that bulbil size affected not only the growth response of seedlings and plants but also the quality of the produced corms. Therefore, the objectives of this experiment were to study the effect of bulbils origin on A. muelleri Blume (Porang) growth and the quality of harvested corms. The quality of corms was assessed based on glucomannan and (CaOx) content. Bulbils from the middle and edge of branches were grown in polybag (f 5 cm) containing compost:fertilizer (1:1) mixed media under 40% shade. Each bulbil origin was repeated 8 times. The results showed that growth responses of plant from middle bulbil were significantly better than that from edge bulbil. However, at the harvesting time, the weight and diameter of corms derived from both types of bulbils were not significantly different. The glucomannan and calcium oxalate (CaOx) content of harvested corms grown from middle bulbil tends to be higher than that from edge bulbil.Keywords: branching, calcium oxalate (CaOx), germination, glucomannan, Porang.
EKSTRAKSI SENYAWA FENOL DAN AKTIVITAS ANTIOKSIDAN DARI BUAH CABAI RAWIT DENGAN METODE MICROWAVE ASSISTED EXTRACTION Kusnadi, Joni; Dedi, Dedi; Yunianta, Yunianta; Arumingtyas, Estri Laras
Jurnal Teknologi Pertanian Vol 18, No 3 (2017)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.172 KB) | DOI: 10.21776/ub.jtp.2017.018.03.18

Abstract

ABSTRAKPertimbangan dalam pemilihan metode ekstraksi untuk mendapatkan senyawa bioaktif dari bahan tanaman diantaranya adalah efisiensi waktu dan jumlah pelarut yang digunakan. Studi terdahulu menunjukan ekstraksi bahan aktif dengan gelombang mikro mempercepat waktu ekstraksindan menghemat penggunaan pelarut dibandingkan metode konvensional, tetapi waktu ekstraksibyang terlalu singkat dan penggunaan pelarut yang terlalu minim membuat hasil ekstraksi tidaknoptimal. Penelitian ini untuk mengetahui pengaruh rasio bahan:pelarut dan lama ekstraksi dengan gelombang mikro terhadap kandungan fitokimia dan aktivitas antioksidan dari ekstrak cabai rawit. Penelitian dilakukan menggunakan Rancangan Acak Kelompok 2 faktor yaitu rasio bahan:pelarut dengan variasi 1:5; 1:10; dan 1:15, serta lama ekstraksi (5, 10, dan 15 menit). Berdasarkan hasil pengujian, rasio bahan:pelarut 1:10 memberikan hasil terbaik untuk kadar total capsaicinoid, total fenol, total flavonoid, dan aktivitas antioksidan dengan nilai rata-rata sebesar 138.06 ± 6.35 mg CE/g BK ekstrak, 33.75 ± 1.75 mg GAE/g BK ekstrak, 205.41 ± 26.13 mg QE/g BK ekstrak, dan 447.96 ±25.63 μg/ml. Lama ekstraksi untuk menghasilkan nilai total capsaicinoid dan total fenol paling tinggi adalah 5 menit, sedangkan untuk total flavonoid pada waktu 10 dan 15 menit. Nilai IC50 memiliki korelasi yang linear dengan total capsaicinoid, total fenol, dan total flavonoid. Pemilihan perlakuan terbaik dengan metode multiple atribut menunjukan rasio bahan:pelarut 1:10 dan lama ekstraksi 10 menit merupakan kombinasi perlakuan paling optimal untuk ekstraksi senyawa fenol menggunakan Microwave Assisted Extraction ABSTRACTThe consideration of extraction methods to obtain bioactive compounds of plant origin were the time efficiency and the amount of extraction solvent. Previous studies showed that microwave assisted extraction of active ingredients decrease the extraction time and minimize the solvent compared to conventional methods, but short extraction time and less solvents make extraction less optimal. The aim of this study to determine the effect of materials:solvent and extraction time of microwave assisted extraction to phytochemical content and antioxidant activity of the cayenne pepper extract. The study was conducted using a Randomized Block Design 2 factors of the ratio of material:solvent (1:5; 1:10; and 1:15) and extraction time (5, 10, and 15 min). The results showed that material:solvent ratio of 1:10 gave the best value for total capsaicinoid content, total phenol, total flavonoid and IC50 with average value of 138.06 ± 6.35 mg CE/g DW, 33.75 ± 1.75 mg GAE/g DW extract, 205.41 ± 26.13 mg QE/g DW extract, and 447.96 ± 25.63 μg/ml respectively. The optimal extraction time to generate total capsaicinoid and total phenol content was 5 minutes, while the optimal extraction time for total flavonoid was 10 and 15 minutes. IC50 in this study had a linear correlation with total capsaicinoid, total phenol, and total flavonoid. The best treatment with multiple attribute method showed the materials:solvent ratio of 1:10 and extraction time of 10 minutes was the most optimal treatment combination for extraction of phenol compound by microwave assisted extraction
Exploration and Morphological Character Identification of Local Durian (Durio zibethinus Murr.) from Tidore Island, North Maluku Sundari, Sundari; Arumingtyas, Estri Laras; Hakim, Luchman; Azrianingsih, Rodiyati
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

Local durian is the name for Durio zibethinus Murr. found in the Tidore Island, North Maluku. There are many local names for local durian in Tidore Island that have many different morphological variation. The objective of this study was to inventarize and analyze the morphological charactervariations of 19 local durian names collected from 9 villages: Gurabunga, Gubukusuma, Afa-afa, Fobaharu, Foralaha, Jaya, Bobo, Dokiri and Rum in the Tidore Island, North Maluku. This research was conducted in March-June 2015 using descriptive exploratory method with purposive sampling. Local durians found in Tidore Island have variations local names and variation of morphological characters include: habitus, leaf shape, fruit shape, flesh color and texture, seedshape, and the shape ofthe aril thorn. Keywords: inventarization; local durian; morphological characters; Tidore Islands
Keragaman Morfologi Kenaf (Hibiscus cannabinus L.) KR 11 Mutan EMS (Ethyl Methanesulfonate) berdasarkan Panduan Karakterisasi Kenaf Hakin, Rikza; Arumingtyas, Estri Laras
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Kenaf (Hibiscus cannabinus L.) merupakan salah satu sumber serat yang cukup potensial untuk dimanfaatkan secara optimal. Peningkatan pemanfaatan tanaman kenaf dapat dilakukan dengan menghasilkan varietas baru kenaf, salah satunya menggunakan EMS. Tujuan dari penelitian ini ialah mengetahui keragaman tanaman kenaf hasil mutasi EMS berdasarkan Panduan Karakterisasi Kenaf yang dikeluarkan oleh Departemen Pertanian. Penelitian dilakukan menggunakan 6 genotip berbeda, yaitu satu control (KR 11) dan lima genotip mutan EMS. Metode yang digunakan ialah melakukan pengamatan pada karakter morfologi yang meliputi karakter kualitatif dan kuantitatif dari karakter daun, bunga maupun karakter batang. Pengamatan karakter daun dilakukan pada saat tanaman berbunga pertama, karakter pembungaan diamati pada saat tanaman mengalami umur berbunga 50% serta karakter batang diamati pada saat panen. Hasil pengamatan kemudian disesuaikan dengan character state yang ditentukan dari Panduan Karakterisasi Kenaf dan dianalisis menggunakan software Clad97. Berdasarkan panduan tersebut, mutan yang memiliki keragaman paling rendah terhadap KR 11 ialah genotip mutan mutan 1 dengan nilai similaritas sebesar 94.4% sedangkan mutan dengan keragaman paling tinggi ditunjukkan oleh genotip mutan 4 dengan nilai similaritas sebesar 67.5%.
GENETIC ANALYSIS AND MOLECULAR INTERACTION OF UGPase IN SOME TUBER PLANTS RELATED TO GLUCOMANNAN PRODUCTION Ulum, Badiul; Arumingtyas, Estri Laras; Kurniawan, Nia
Biotropika: Journal of Tropical Biology Vol 1, No 3 (2013)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

UGPase is an enzyme that affect the conformation of polysaccharide with β 1-4 bond. There are some differences interaction of UGPase enzyme with UDP-Glucose substrate in some tuber plants. So, the aim of this research are to observe the interaction of UGPase and UDP-G in some tuber plants and to observe the relationship among tuber plant based on UGPase sequence. Result of molecular docking shows the catalytic domain interaction of UGPase and UDP-G in substrate binding loop, lower energy free obtain from A. konjac is -5.06 kcal/mol, following by I. batatas and S. tuberosum is -4,60 dan -3,73 kcal/mol respectively. Interaction was stabilize by some interaction type, there are electrostatic and hydrogen in A. konjac and I. batatas, and hydrophobic in S. tuberosum. Sequence alignment of UGPase shows I. batatas and S. tuberosum relatively close than to A. konjac.   Key words: interaction, UDP-Glucose, UGPase
Mapping Potensi Produk Olahan Untuk Menunjang Kemandirian Desa Wolowea Timur Kecamatan Boawae Kabupaten Nagekeo Provinsi NTT Kusnadi, Joni; Winarsih, Sri; Utomo, Edi Priyo; Arumingtyas, Estri Laras; Hidayat, Noor
Journal of Food and Life Sciences Vol 2, No 2 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.63 KB) | DOI: 10.21776/ub.jfls.2018.002.02.01

Abstract

Wilayah Indonesia bagian timur, tepatnya yaitu Nusa Tenggara Timur merupakan wilayah yang memiliki permasalahan dengan pengelolaan hasil alam yang berdampak pada perekonomian, sosial dan budaya. Menurut data statistik BPS 2016 menunjukkan bahwa jumlah penduduk miskin dipedesaan mencapai 22,01%. Selain itu Indeks Desa Membangun (IDM) provinsi Nusa Tenggara Timur adalah 0.538, di bawah rata-rata nasional 0,566 dan jauh dari provinsi terdekatnya yaitu NTB yang mencapai sebesar 0,618. Kabupaten Nagekeo memiliki IDM sebesar 0,5286 di bawah nilai IDM Provinsi. Desa Wolowea Timur yang merupakan desa pemekaran belum memiliki nilai IDM sehingga perlu dilakukan pemetaan yang lebih baik. Serta Mapping sektor pengelolaan hasil alam, perekonomian dan kebudayaan patut dilakukan untuk menciptakan kesejahteraan dan kemakmuran bagi penduduk di daerah tertinggal. Mapping sektor dilakukan berdasarkan pengelompokkan (klaster) komoditas pertanian dan perkebunan kemudian dilanjut dengan skala prioritas potensi olahan produk berdasarkan 4 indikator produktivitas (0,20), produk olahan (0,20), kemampuan oalahan (0,30), serta nilai jual (0,30). Mapping sektor pengembangan produk olahan dihasilkan terdapat 2 komoditas yang sangat berpotensi untuk dikembangkan, yaitu komoditas jagung produk olahan marning jagung dengan nilai prioritas 8 dan komoditas kopi produk kopi bubuk (ground) dengan nilai prioritas 8.4. Selain itu masyarakat desa Wolowea Timur Kecamata Boawae memiliki komoditas andalan yakni kemiri akan tetapi mereka masih memiliki kendala dalam penanganan pasca panen sehingga perlu ada pelatihan dan pengetahuan untuk bisa mengatasi hal tersebut.Kata Kunci: Desa Wolowea Timur, Kecamatan Boawae, Kabupaten Nagekeo, Provinsi NTT Mapping, Potensi, Produk Olahan .
KERAGAMAN DAN KEKERABATAN KULTIVAR JAGUNG (Zea mays L.) LOKAL ASAL PULAU KISAR KABUPATEN MALUKU BERDASARKAN KARAKTER FENOTIP Hermalina Sinay; Estri Laras Arumingtyas; Nunung Harijati; Serafinah Indriyani
BIOPENDIX Vol 3 No 1 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue1page18-27

Abstract

Background: The relationship of plant kinship can be done through the identification of the phenotype character that is the character that appears on the individual or plant organism that can be both quantitative and qualitative. The purpose of this research is to know the diversity and kinship of corn cultivar from Kisar Island Regency of Maluku Southwest of Maluku Province based on phenotype character. Methods: The genetic material used was six local corn cultivars and one comparative variety. The six local corn cultivars are Red ruby with a brown cob, Blood red corn, Sticky, Ripe yellow, Deep yellow, and White, while the varieties are Srikandi varieties. To know the effect of cultivars and corn varieties on phenotypic diversity, variant analysis with Statistica Analytical System (SAS version 9.0) was used. To know the character that gives the greatest contribution to the diversity of cultivar phenotype and corn varieties, the main component analysis is done. To know the kinship among corn cultivars based on phenotypic character, gross analysis (cluster analysis) with hierarchy method is performed. The main component analysis and gameplay analysis was performed using SPSS version 18.0 computer program. Results: The results of variance analysis showed a significant effect of maize cultivar on phenotypic diversity. Characters that contribute to the diversity of the corn cultivar phenotype are the proline level, the weight of cobs without harvesting at harvest, the weight of cobs without dryness of the oven, the weight of the cobs at 12% moisture content, the unleaded cobs at 12%, the diameter of the ear, the number of seeds per ear, the number of rows of beans per ear, the number of leaves, and the length of the leaf. The results of Euclidean and Euclidean girder analysis show that the local yellow cultivars are in one group alone and have a low similarity with other local corn cultivars as well as the Srikandi-based varieties based on their phenotypic characters. Conclusion: There is a variety of phenotypes of local corn cultivars and varieties of Srikandi compounds grown on Kisar Island of Southwest Maluku Maluku Province.
Keragaman Morfologi Kenaf (Hibiscus cannabinus L.) KR 11 Mutan EMS (Ethyl Methanesulfonate) berdasarkan Panduan Karakterisasi Kenaf Rikza Hakin; Estri Laras Arumingtyas
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kenaf (Hibiscus cannabinus L.) merupakan salah satu sumber serat yang cukup potensial untuk dimanfaatkan secara optimal. Peningkatan pemanfaatan tanaman kenaf dapat dilakukan dengan menghasilkan varietas baru kenaf, salah satunya menggunakan EMS. Tujuan dari penelitian ini ialah mengetahui keragaman tanaman kenaf hasil mutasi EMS berdasarkan Panduan Karakterisasi Kenaf yang dikeluarkan oleh Departemen Pertanian. Penelitian dilakukan menggunakan 6 genotip berbeda, yaitu satu control (KR 11) dan lima genotip mutan EMS. Metode yang digunakan ialah melakukan pengamatan pada karakter morfologi yang meliputi karakter kualitatif dan kuantitatif dari karakter daun, bunga maupun karakter batang. Pengamatan karakter daun dilakukan pada saat tanaman berbunga pertama, karakter pembungaan diamati pada saat tanaman mengalami umur berbunga 50% serta karakter batang diamati pada saat panen. Hasil pengamatan kemudian disesuaikan dengan character state yang ditentukan dari Panduan Karakterisasi Kenaf dan dianalisis menggunakan software Clad97. Berdasarkan panduan tersebut, mutan yang memiliki keragaman paling rendah terhadap KR 11 ialah genotip mutan mutan 1 dengan nilai similaritas sebesar 94.4% sedangkan mutan dengan keragaman paling tinggi ditunjukkan oleh genotip mutan 4 dengan nilai similaritas sebesar 67.5%.
GENETIC ANALYSIS AND MOLECULAR INTERACTION OF UGPase IN SOME TUBER PLANTS RELATED TO GLUCOMANNAN PRODUCTION Badiul Ulum; Estri Laras Arumingtyas; Nia Kurniawan
Biotropika: Journal of Tropical Biology Vol 1, No 3 (2013)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

UGPase is an enzyme that affect the conformation of polysaccharide with β 1-4 bond. There are some differences interaction of UGPase enzyme with UDP-Glucose substrate in some tuber plants. So, the aim of this research are to observe the interaction of UGPase and UDP-G in some tuber plants and to observe the relationship among tuber plant based on UGPase sequence. Result of molecular docking shows the catalytic domain interaction of UGPase and UDP-G in substrate binding loop, lower energy free obtain from A. konjac is -5.06 kcal/mol, following by I. batatas and S. tuberosum is -4,60 dan -3,73 kcal/mol respectively. Interaction was stabilize by some interaction type, there are electrostatic and hydrogen in A. konjac and I. batatas, and hydrophobic in S. tuberosum. Sequence alignment of UGPase shows I. batatas and S. tuberosum relatively close than to A. konjac.   Key words: interaction, UDP-Glucose, UGPase