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Hubungan Ekspresi BRAF V600E dengan Varian Histopatologik dan Infiltrasi Limfosit pada Papillary Thyroid Carcinoma (PTC) RZ Nizar; Dutia Gestin; Aswiyanti Asri; Husna Yetti
Majalah Patologi Indonesia Vol 30 No 2 (2021): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.975 KB) | DOI: 10.55816/mpi.v30i2.473

Abstract

BackgroundPapillary thyroid carcinoma is the most common endocrine gland malignancy that has the highest incidence rate of 60-80%. In papillarythyroid carcinoma BRAF V600E mutation plays an important role in carcinogenesis. BRAF V600E is a RAS-regulated-serine-threoninekinase and activator of the MAPK pathway cascade that functions to regulate cell proliferation, differentiation and survival. BRAFV600E mutations in PTC is associated with a high incidence of recurrence and reduced survival. The purpose of this study is to lookat the relationship of BRAF V600E expression with histopathological variants and lymphocyte infiltration in PTC.MethodsThis research was a retrospective observational cross sectional study with 40 cases of papillary thyroid carcinoma which werediagnosed in 4 Pathology Anatomy Laboratory in West Sumatera. Samples were obtained from paraffin blocks of tissue from surgeryand were reevaluated for histopathological variant and lymphocyte infiltration. Expression of BRAF V600E in tumor cell were analyzedusing immunohistochemistry staining. Bivariate statistical analysis of Chi-Square were used and p value <0,05 were consideredsignificance.ResultsPositive BRAF V600E expression was found in 24 case (60%). Positive BRAF V600E expression was mostly found in classic PTC(50%). Papillary thyroid carcinoma with lymphocyte infiltration mostly found with negative BRAF V600E expression (79.2%). Statisticalanalysis showed no significant correlation between BRAF V600E with histopathological variant (p=0.74) and no significancecorrelation between BRAF V600E expression with lymphocyte infiltration (p=0.12).ConclusionThe conclusion is BRAF V600E expression has no correlation with histological variants in papillary thyroid carcinoma and no significantcorrelation of BRAF V600E expression with lymphocyte infiltration.
Hubungan Grading Histopatologi dan Infiltrasi Limfovaskular dengan Subtipe Molekuler pada Kanker Payudara Invasif di Bagian Bedah RSUP. Dr. M. Djamil Padang Vashti Resti Putri Firdaus; Aswiyanti Asri; Daan Khambri; Wirsma Arif Harahap
Jurnal Kesehatan Andalas Vol 5, No 1 (2016)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v5i1.463

Abstract

AbstrakGejala kanker payudara sering tidak disadari atau dirasakan dengan jelas oleh penderita, sehingga banyak penderita yang datang dalam keadaan stadium lanjut. Banyak faktor yang mempengaruhi prognosis dari kanker payudara, antara lain grading histopatologi, reseptor estrogen dan progesteron, HER2, serta infiltrasi limfovaskular. Tujuan penelitian ini adalah menentukan hubungan antara grading histopatologi dan infiltrasi limfovaskular terhadap subtipe molekuler pada kanker payudara invasif. Distribusi frekuensi dari grading histopatologi, infiltrasi limfovaskular, dan subtipe molekuler pada kanker payudara invasif di bagian bedah RSUP.Dr. M. Djamil Padang selama periode 1 Januari 2010 – 31 Desember 2013. Metode penelitian ini adalah observational analitik, dengan pendekatan cross sectional. Dalam penelitian ini didapatkan 424 kasus kanker payudara invasif, dengan 66 kasus yang dapat dianalisis. Analisis data yang digunakan univariat dan bivariat dengan menggunakan uji chi-square dengan derajat kemaknaan p<0,05. Ada hubungan yang bermakna antara grading histopatologi dengan subtipe molekuler (p=0,032). Hubungan infiltrasi limfovaskular dengan subtipe molekuler pada kanker payudara invasif didapatkan bermakna (p=0,000).Kata kunci: grading histopatologi, infiltrasi limfovaskular, subtipe molekuler, kanker payudara invasif AbstractBreast cancer symptoms are often not recognized or clearly perceived by the patient, so it makes most of patients come to doctor in late stage. There are many prognosis factors in breast cancer, such as hystopathology grading, estrogen and progesteron receptors, HER2, and lymphovascular infiltration.The objective of this study was to determine the association between hystopathology grading and lymphovascular infiltration with molecular subtype in invasive breast cancer. Determine the distribution and frequency of hystopatology grading, lymphovascular infiltration, and molecular subtype in invasive breast cancer at surgery department Dr. M. Djamil Hospital Padang period January 1, 2010 – December 31, 2013. The method of this research is analitic observational with cross sectional study. In this research obtained 424 cases of invasive breast cancer, with only 66 cases that could be analyzed. The analysis system that used is univariat and bivariat with chi-square (p<0,05). There is significant correlation between hystopathology grading with molecular subtype (p=0,032). There is significant correlation between lymphovascular infiltration with molecular subtype (p=0,000).Keywords: histopathology grading, lymphovascular infiltration, molecularsubtypes, invasive breast cancer
Efek Pemberian Vitamin C terhadap Gambaran Histopatologi Hati Tikus Wistar yang Terpapar Timbal Asetat Arifuddin Arifuddin; Aswiyanti Asri; Elmatris Elmatris
Jurnal Kesehatan Andalas Vol 5, No 1 (2016)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v5i1.471

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AbstrakPaparan timbal asetat dapat menyebabkan kerusakan sel hati dan menurunkan aktivitas enzim yang bekerja sebagai antioksidan esensial dalam tubuh. Vitamin C merupakan senyawa alami yang bersifat antioksidan kuat dan pengikat radikal bebas.Tujuan penelitian ini adalah menentukan efek pemberian vitamin C terhadap gambaran histopatologi hati tikus wistar yang terpapar plumbum asetat dan dilakukan di laboratorium Fakultas Farmasi dan laboratorium Patologi Anatomi Fakultas Kedokteran Universitas Andalas. Penelitian ini dilakukan pada 28 ekor tikus wistar jantan, berumur 3 bulan, berat badan ± 200 gr. Didapatkan bahwa pada pemaparan timbal 50 mg/kgBB/hari selama 4 minggu meningkatkan skor Manja Roenigk hati tikus wistar. Penambahan vitamin C 50 mg/kgBB/hari dan 75 mg/kgBB/hari pada tikus yang terpapar timbal asetat menunjukkan penurunan skor Manja Roenigk pada hati tikus wistar. Berdasarkan uji t berpasangan ternyata tidak terdapat perbedaan yang signifikan antara skor Manja Roenigk yang tidak terpapar dan terpapar timbal asetat (p = 0,501). Berdasarkan uji one way Anova terdapat pengaruh yang signifikan vitamin C terhadap gambaran histopatologi hati tikus wistar (p = 0,000). Secara statistik pemberian kedua dosis vitamin C ini tidak memberikan perbedaan yang bermakna (p = 0.379).Kesimpulan penelitian ini adalah terdapat pengaruh proteksi vitamin C terhadap mikroskopis hati tikus wistar yang telah terpapar timbal asetat.Kata kunci: Timbal Asetat, Gambaran Histopatologi Hati, Vitamin C, Plumbum Asetat, Manja Roenigk AbstractExposure of lead acetate can caused cell injury in liver and decrease activity of enzyme which works as essential antioxidant in body. Vitamin C is a natural compound as potent antioxidant and free radical binding.The objective of this study was to determine the effect of vitamin C on liver histopathology in exposed lead acetate wistar rats. This research was conducted at Pharmacy Laboratory and Pathology laboratory, Faculty of Medicine, Andalas University. The study performed on 28 male Wistar rats, 3 months old, body weight 200 g.             It was found that 50 mg/ kgBW/day of lead exposure for 4 weeks increased Manja Roenigk’s grading of wistar rat’s liver. Administration of vitamin C 50 mg/kgBW/day and 75 mg/kgBB/day to rat which exposed to lead acetate showed decrease in score of Manja Roenigk on wistar rat’s liver. Based on paired sample t test was not significant difference score of Manja Roenigk between unexposed and exposed lead acetate (p = 0.501). Based on one way Anova test was found  a significant effect of vitamin C to histopathology of wistar rat’s liver (p = 0.000). Statistically administration of both dose vitamin C did not give significant difference (p = 0.379).The conclusion of this study show  there is the effect of protection from administration of vitamin C to microscopic Wistar rat liver which exposed to lead acetateKeywords: lead acetate, histopathology of liver, vitamin C, plumbum acetate, Manja Roenigk
Korelasi antara Latent Membrane Protein-1 Virus Epstein-Barr dengan P53 pada Karsinoma Nasofaring (Penelitian Lanjutan) Yenita .; Aswiyanti Asri
Jurnal Kesehatan Andalas Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v1i1.1

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Abstrak Latar Belakang: Karsinoma nasofaring (KNF) merupakan tumor yang unik karena etiologi dan distribusi endemiknya. Di daerah endemik, etiologi KNF berkaitan dengan infeksi EBV. Infeksi EBV yang laten dan persisten pada KNF menunjukkan pola laten tipe II yang ditandai dengan ekspresi EBNA-1, LMP-1, 2 dan EBER. LMP-1 merupakan gen laten EBV yang pertama ditemukan yang dapat mentransformasi galur sel, merubah fenotip sel, menginduksi proliferasi dan mencegah apoptosis. Tujuan dari penelitian ini adalah mengetahui korelasi antara ekspresi LMP-1 EBV dengan ekspresi p53 pada KNF. Cara: Empat puluh sembilan slaid HE dan blok parafin dari KNF dianalisis dan dipulas secara imunohistokima dengan antibodi LMP-1 EBV dan p53. Korelasi antara ekspresi LMP-1 dengan ekspresi p53 diuji dengan menggunakan uji Korelasi Pearson. Nilai p
Karakteristik Klinis dan Patologis Karsinoma Nasofaring di Bagian THT-KL RSUP Dr.M.Djamil Padang Shofi Faiza; Sukri Rahman; Aswiyanti Asri
Jurnal Kesehatan Andalas Vol 5, No 1 (2016)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v5i1.450

Abstract

AbstrakKarsinoma nasofaring banyak terjadi di Cina dan Asia Tenggara, termasuk di Indonesia, sering didiagnosis pada keadaan lanjut dan memiliki prognosis yang buruk. Tujuan penelitian ini adalah untuk mengevaluasi epidemiologi, karakteristik klinis, dan tipe histopatologi pada pasien karsinoma nasofaring di Bagian THT-KL. Metodologi penelitian ini adalah deskriptif dengan menggunakan data rekam medik di RSUP Dr. M. Djamil Padang selama Juni 2010 sampai Juli 2013 dan data hasil pemeriksaan histopatologi sebagai konfirmasi.  Didapatkan sebanyak 44 kasus yang lengkap pada periode tersebut, yang mana 52,27% penderita adalah laki-laki dan 47,22% perempuan, perbandingan laki-laki dan perempuan adalah 1,2 : 1. Sebaran umur penderita dari 17 sampai 75 tahun dengan insiden puncak pada umur 41- 65 tahun. Gejala klinis terdiri atas massa di leher 93,17%, diikuti dengan obstruksi nasal 79,55%, dan gangguan pendengaran 79,55% sedangkan tanda klinis terdiri atas pembesaran kelenjar getah bening leher 90,91%, diikuti dengan tuli 79,55%, cranial nerve palsy  dan perluasan kelenjar getah bening ke fossa supraklavikula masing-masing 15,8%. Sebagian besar pasien berada pada stadium IV 83,16%, dengan derajat tumor terbanyak T4N2M0 15,91%. Tipe histopatologi yang terbanyak adalah nonkeratinizing carcinoma, undifferentiated type 75%, diikuti keratinizing SCC 13,64%, dan nonkeratinizing carcinoma - differentiated type 11,36%.Kata kunci: karsinoma nasofaring, karakteristik klinis, histopatologi, padang AbstractNasopharyngeal carcinoma  is more frequent in China and Southeast Asia, including Indonesia, commonly with advance stages at diagnosis and has a poor prognosis.  The objective of this study was to evaluate epidemiology, clinical characteristic and histopathology types of patients with nasopharyngeal carcinoma in the department of Otorhinolaryngology - Head and Neck Surgery.This is a descriptive study that used data from medical record of Dr. M. Djamil General Hospital in Padang during June 2010 to July 2013 and histopathology examination as confirmation. The result demonstrated 44 cases found on that period, of which 52,27% was male and 47,72% was female, hence the male and female ratio was 1,2 : 1.  The age-range from 17 to 75 years old with incidence peak between 41 - 65 years old. Clinical symptoms were neck mass 93,17%, followed by nasal obstruction 79,55 %, and audiological complaints 79,55% while clinical sign were cervical lymphadenopathy 90,91%, followed by hearing loss 79,55%, cranial nerve palsy and lymphadenopathy metastases to fossa supraclavicular each subject 15,8%. Most of patients were classified as stage IV 83,16%, with T4N2M0 15,91%.  The histopathology type were nonkeratinizing carcinoma, undifferentiated type had percentage 75%, followed by keratinizing SCC 13,64%, and nonkeratinizing carcinoma - differentiated type 11,36%. Keywords: nasopharyngeal carcinoma, clinical characteristic, histopathology types, padang
Profil Tumor Ganas Ovarium di Laboratorium Patologi Anatomi Fakultas Kedokteran Universitas Andalas Periode Januari 2011 Sampai Desember 2012 Iwani Rahmah Rambe; Aswiyanti Asri; Adrial Adrial
Jurnal Kesehatan Andalas Vol 3, No 1 (2014)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v3i1.26

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AbstrakTumor ganas ovarium menempati urutan ketiga setelah tumor ganas serviks dan tumor ganas payudara yang menyebabkan kematian di Indonesia. Penelitian ini didapatkan 143 kasus tumor ganas ovarium. Distribusi profil tumor ganas ovarium berdasarkan umur terbanyak pada kelompok umur 31-40 tahun sebanyak 45 kasus (31,46%), berdasarkan jumlah paritas yang terbanyak adalah pada kelompok paritas 1-3 sebanyak 77 kasus (66,37%), berdasarkan status perkawinan yang terbanyak ditemukan pada perempuan yang sudah menikah sebanyak 116 kasus (81,11%), berdasarkan sel asal tumor terbanyak ditemukan dari sel epitel sebanyak 137 kasus (95,80%), dan berdasarkan subtipe histopatologi yang terbanyak adalah kistadenokarsinoma ovarii serosum sebanyak 72 kasus (50,35%).Kata kunci: tumor ganas ovarium, paritas, sel asal tumorAbstractMalignant ovarian tumour is third rank after malignant cervic tumour and malignant breast tumour that cause death in Indonesia. The research found 143 cases of malignant ovarian tumours. Distribution profile of malignant ovarian tumours by the age of majority in the age group 31-40 years were 45 cases (31,46%), based on the highest number of parity is the parity group 1-3 were 77 cases (66,37%), based on marital status which is mostly found in women who are married as many as 116 cases (81,11%), based on the origin of the tumour cells were observed on the epithelial cells by 137 cases (95,80%), and based on the histopathological subtype is most serosum ovarian cystadenocarcinoma were 72 cases (50,35%).Keywords: malignant ovarian tumours, parity, origin of the tumor
Hubungan Ekspresi IGF-1R dan HER-2 Terhadap Jumlah Mitosis dan Derajat Histopatologik pada Karsinoma Payudara Invasif Tidak Spesifik Yessy Setiawati; Aswiyanti Asri; Rosfita Rasyid
Majalah Patologi Indonesia Vol 29 No 3 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.902 KB) | DOI: 10.55816/mpi.v29i3.442

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BackgroundThe IGF-1R signaling pathways have cross-talk with HER-2 signaling pathways and was thought to be one of the resistance mechanism intrastuzumab therapy. This research was proposed to analyze the correlation between IGF-1R and HER-2 expression with mitosis count andhistopathological grade in invasive breast carcinoma of no special type.MethodsThis research was a cross-sectional design. A total of fifty-five invasive breast carcinoma of no special type cases diagnosed in theAnatomical Pathology Centre Diagnostic of Medical Faculty of Andalas University in a period of 2014-2015 were collected and stainedimmunohistochemically with IGF-1R and HER-2 antibodies. IGF-1R and HER-2 expression were examined and their correlation with mitosiscount and histopathological grade were statistically analyzed with T-test, Oneway Anova and Chi-Square test.ResultsIGF-1R cytoplasm and membranous expression were found positive in 18.2% and 34.5% cases respectively, meanwhile, HER-2 expressionswere found positive in 23.6% cases of invasive breast carcinoma of no special type. There were significant correlations between IGF-1Rcytoplasm expression with mitosis count (p=0.049). There were no significant correlations between IGF-1R membranous expression withmitosis count (p=0.641) with histopathological grade (p=1.00). There were no significant correlations between HER-2 expression withmitosis count (p=0,495) and histopathological grade (p=1.000).ConclusionIGF-1R expressions have a significant correlation with mitosis count rather than HER-2 expressions. Inhibition it’s signaling pathways mayhave therapeutic value in breast carcinoma. Combination therapy of anti-HER-2 and anti-IGF-1R are expected to overcome resistance withtrastuzumab in HER-2 positive breast carcinoma
Ekspresi pRb untuk Membedakan Neoplasma Tiroid Jinak dan Ganas Dibandingkan dengan Gambaran Hsitopatologi Laila Isra; Aswiyanti Asri; Edison -
Majalah Patologi Indonesia Vol 22 No 1 (2013): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.278 KB)

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ABSTRAK Latar belakang Neoplasma tiroid sebagai salah satu neoplasma kelenjar endokrin dibagi menjadi neoplasma jinak dan neoplasma ganas (karsinoma).1 Pada pemeriksaan histopatologi tiroid rutin dengan pewarnaan HE (hematoksilin-eosin) sering ditemukan kesulitan untuk membedakan antara neoplasma jinak dengan neoplasma ganas, terutama antara adenoma folikuler dan karsinoma folikuler.2,3 Cara menilai pemeriksaan histopatologi dilakukan dengan melihat invasi sel-sel tumor ke dalam kapsul.1-4 Protein Rb-1 (pRb-1) merupakan petanda tumorsuppressor retinoblastoma yang berfungsi sebagai cell cycle check points. Peristiwa mutasi gen pRb menunjukkan bahwa sel sudah kehilangan kemampuan untuk mengontrol siklus sel, sehingga mutasi gen Rb dapat ditemukan pada sel tumor ganas.5,6,7 Penelitian ini bertujuan untuk mengetahui hubungan antara ekspresi Rb-1 dengan jenis dan gambaran histopatologik neoplasma tiroid. Metode Jenis penelitian observasional dengan desain cross sectional menggunakan 60 sampel neoplasma tiroid. Reevaluasi dilakukan pada preparat HE. Pada blok parafin yang telah dipotong, dilakukan pemeriksaan imunohistokimia menggunakan antibodi Rb-1. Labelling index dihitung dengan persentase sel yang positif 10% (positif) dan neoplasma tiroid jinak menunjukkan ekspresi Rb-1
Penurunan Aktivitas Proliferasi Sel Adenokarsinoma Mamma Mencit C3H akibat Pemberian Ekstrak Etanol Lengkuas (Alpinia galanga) Susanto Winarko; Yanwirasti .; Aswiyanti Asri; Salmiah Agus
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.318 KB)

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Background WHO data in 2011 showed that deaths from breast cancer in Indonesia reached 20,052 peoples of all deaths. This is partly due to the high cost of treatment, so that many people are turning to alternative therapies such as traditional plants. Lengkuas (Alpinia galanga (L) Wild is the traditional material that is cheap and easily available in Indonesia. Lengkuas-containing active substances I-acetoxychavicol acetate (ACA), and according to past research has anti-cancer properties by inhibiting the activity through the inhibition of cell proliferation pathways nuclear factor kappa B (NFƙB). To investigate the effect of ethanol extract of lengkuas against mammary adenocarcinoma cell prolifferation activities in C3H mice with various dose levels. Methods This study was an experimental study using C3H mice with post-test only research design controlled group design. Experimental animals consisted of 32 C3H mice were inoculated with tumor cells and then divided into 4 groups: control group (K) and three treatment groups (P1, P2 and P3) were given graded doses of ethanol extract of lengkuas 225 mg/kgBW/day, 450 mg/kgBW/day and 675 mg/kgBW/ day for 2 weeks. After all mice were terminated, examined the proliferative activity of tumor cells with Ki-67 antibody. Differences in proliferative activity between the groups were tested using one-way anova followed by post hoc Bonferroni test. Results There were significant differences in proliferative activities (p=0.000) among the four groups. By the post hoc Bonferroni analysis was found the significant differences in proliferative activities between the control group P1, with P2 controls, P3 control group, between P1 with P3 group, P2 with P3 group (p=
INHIBISI AKTIVITAS PROLIFERASI SEL DAN PERUBAHAN HISTOPATOLOGIS MUKOSA WISTAR DENGAN PEMBERIAN PERASAN SELEDRI Aswiyanti Asri
Majalah Kedokteran Andalas Vol 32, No 2: Agustus 2008
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.678 KB) | DOI: 10.22338/mka.v32.i2.p%p.2008

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AbstrakTelah dilakukan penelitian mengenai pengaruh pemberian perasan seledri pada tikus wistar yang diinduksi karsinogenesis kolon.Penelitian ini bertujuan untuk membuktikan bahwa seledri dapat mencegah karsinogenesis kolon pada tikus wistar yang diinduksi dengan 1,2 dimethylhydrazine (DMH) dengan dan tanpa diet tinggi lemak dan tinggi protein.Penelitian ini adalah penelitian eksperimental dengan desain randomized post test control group. Subyek penelitian adalah 25 ekor tikus wistar jantan berusia 12 minggu yang dibagi menjadi 5 kelompok. Kelompok I mendapat injeksi 1,2 DMH subkutan; kelompok II mendapat injeksi 1,2 DMH dan seledri per oral ; kelompok III mendapat injeksi 1,2 DMH subkutan dan diet tinggi lemak dan protein sedangkan kelompok IV selain mendapat injeksi 1,2 DMH subkutan dan diet tinggi lemak dan protein, juga diberi seledri per oral. Perlakuan untuk kelompok I –IV diberikan selama 12 minggu. Sedangkan kelompok V mendapat 1,2 DMH dan seledri selama 16 minggu.Setelah masa perlakuan berakhir, semua tikus dimatikan dan usus besar diambil. Untuk menilai perubahan histopatologik salah satu potongan diproses dan diwarnai dengan HE. Potongan yang lain diwarnai dengan teknik argirofilik dari Ploton untuk analisis aktivitas proliferasi sel. Perubahan histopatologis dinilai secara mikroskopik sesuai kriteria WHO sedangkan aktivitas proliferasi sel dinilai dari jumlah titik AgNOR.Perubahan histopatologik menunjukkan bahwa tikus yang diinduksi dengan 1,2 DMH atau disertai diet tinggi lemak dan protein mengalami perubahan morfologik dan displasia yang lebih berat dibanding tikus yang diinduksi dan diberi seledri.Analisis statistik memakai uji Mann-Whitney didapatkan perbedaan bermakna aktivitas proliferasi sel antara kelompok yang diberi 1,2 DMH dan seledri dengan kelompok yang hanya diberi 1,2 DMH. Pemberian seledri mampu menghambat perubahan histopatologis dan aktivitas proliferasi argrophylic nucledar regions (AgNOR) sel epitel mukosa pada tikus yang diinduksi karsinogenesis kolon dengan 1,2 DMH atau disertai diet tinggi lemak dan protein.Kata Kunci : Karsinogenesis kolon, Seledri, 1,2 DMH, Diet tinggi lemak dan proteinPerubahan histopatologis, hitung AgNORAbstractHas been done research on the effect of celery juice to wistar rats that induced for colon carcinogenesis. Specifically, the aim of this research is to prove celeryARTIKEL PENELITIAN181could prevent the colon carcinogenesis of wistar rats which induced while 1.2 DMH in condition with or without high fat high protein dietary.This study was an experimental study with randomized post test control group. Design totally 25 male wistar rats, aged 12 weeks which randomly divided into five groups, each group consist of 5 rats. Each group were treated by s.c injection of 20 mg/kgBW 1.2 DMH which had been injected once a week. Groups II, IV and V had orally been given a juice celery everyday. Whereas, groups III and IV were fed with the high fat high protein diet,ad libitum. Such above treatment mentioned was treated to all of groups I to IV for 12 weeks, but group V treated for 16 weeks.After the limit of this treatment was finished, all rats were terminated, and large intestines were resected. For the purpose of the histopathological evaluation one of cut sections processed by histological procedures regularlyand stained with H&E. The other of sections was stained by using the argyrophilic techniques as described by Ploton to analyses the activity of cell proliferation. Histopathological pattern in form of epithelial changes had been evaluated by using WHO’s criterias. AgNOR dots were calculated in the nucleus of 100 epithelial cells by using high power fields with emersion oil.The results of histopathological changes indicated the rats induced by 1.2 DMH only or with high fat high protein diet demonstrated a higher degren on morphological changes and dysplasia when it was compared with the group of rats given by celery beside carcinogenic agents. By having analyzed to group I against group II by using Mann-Whittney U-test, it significant differences for cell proliferative activity. This study concluded that celery was effective to prevent histopathological changes and decrease the activity of cell proliferation (AgNOR) in 1.2 DMH induced colon tumors.Keywords: Colon carcinogenesis, celery, 1.2 DMH, high fat high protein diet, histopathological changes, AgNOR count