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All Journal Journal of Parasite Science Ovozoa: Journal of Animal Reproduction

Sunaryo Hadi Warsito

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Author-ID : 2358949

Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Identification and Prevalence of Blood Protozoa on Culling Layer Hen Sold in Wonokromo Traditional Market Surabaya City Moch. Rizky Darmawan; Poedji Hastutiek; Arimbi Arimbi; Sunaryo Hadi Warsito; Dian Ayu Permatasari; Ririn Ririn
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

The most common types of blood protozoa in chickens are from the phylum of Apicomplexa, including Plasmodium, Leucocytozoon and Haemoproteus. Blood protozoan diseases can cause decreased egg production, stunting and death in poultry farms. This study aims to identify and calculate the prevalence of blood protozoan infection in casted off laying chickens sold at Wonokromo Market, Surabaya City. One hundred blood samples from casted off laying chickens were taken by purposive sampling method. Blood samples were collected in Ethylene Diamine Tetra Acetate (EDTA) tubes, and then taken to the Parasitology Laboratory to make a blood smear which was then examined using a microscope with a magnification of 1000x. The results showed that the single infection was 43% positive for Plasmodium sp., 3% positive for Leucocytozoon sp. and 3% for mixed infections (Plasmodium sp. and Leucocytozoon sp.), with a total prevalence of positive samples infected with blood protozoa was 49%.

KORELASI ANTARA LINGKAR DADA, PANJANG BADAN DAN TINGGI GUMBA TERHADAP VOLUME SEMEN DAN KONSENTRASI SPERMATOZOA SAPI MADURA Dilla Aranda Mayola; Rimayanti Rimayanti; Sunaryo Hadi Warsito; Suzanita Utama; Hana Eliyani; Erma Safitri
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The aim of this research was to know the correlation between heart girth, body length, and wither’s height with semen volume and sperm concentration of Madura Bulls. Ten Madura bulls aged three to four years from Madura were measured. Variables observed were heart girth, body length, wither’s height, semen volume and sperm concentration. Data were analyzed by multiple linear regression analysis. The result of this research showed that there was no correlation between heart girth, body length, and wither’s height with semen volume Y = 25,212 + 0,190 X1+ (-0,131) X2 + 0,136 X3, so as there was no correlation between heart girth, body length and wither’s height with sperm concentration Y = 8739,559 + (-36,663) X1+ (19,714) X2 + 6,2960 X3. And than was no correlation between semen volume and sperm concentration Y = 7,303 + (-0,002) X1.

PERANAN VITAMIN B12 DALAM PENGENCER SUSU SKIM KUNING TELUR TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA DOMBA SAPUDIPOST THAWING Mirza Asafaizh Arini; Tri Wahyu Suprayogi; Sunaryo Hadi Warsito; Trilas Sardjito; Suherni Susilowati; Indah Norma Triana
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to prove that the addition of Vitamin B12 in egg yolk skim milk extender can maintain the motility and viability of post thawing Sapudi sheep spermatozoa. This study used sample of Sapudi sheep fresh semen that was collected with artificial vagina, and divided into 3 different treatments. The control treatment or P0, Sapudi sheep semen was diluted in the egg yolk skim milk extender + vitamin C 0,02 mg / ml, treatment group (P1 and P2), Sapudi sheep semen was diluted in the egg yolk skim milk extender+ vitamin B12 5 μg / ml and grouped in P1, Sapudi sheep semen was diluted in the egg yolk skim milk extender + Vitamin C 0.02 mg / ml + Vitamin B12 5μg / ml and grouped in P2. The data analysis was use One Way Analysis of Variant (ANOVA), then to identify the real difference among the treatments continued with Duncan distance test. The result of data analysis showed that P2 treatment was significantly different (p <0.05) with P1 treatment and P0 treatment or control. While P1 treatment was not significantly different (p> 0,05) with P0 treatment or control. The result showed that the addition of vitamin C 0.02 mg / ml with vitamin B12 5 μg / ml combination in egg yolk skim milk extender was the best addition to maintain the motility and viability of the post thawing Sapudi sheep spermatozoa.

PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN Maulinda Agchirilia Vladika; Sunaryo Hadi Warsito; Suherni Susilowati; Tatik Hernawati; Rochmah Kurnijasanti; Tjuk Imam Restiadi
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

The purpose of this study was to determine the effect and the best concentrasion of green tea extract in skim milk diluent for motility, viability, and intact plasma membrane Sapudi sheep spermatozoa that was stored 50C. The semen was divided into four groups: skim milk diluent; 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Spermatozoa quality was observed first day until fifth day after diluent. The data obtained was analyzed with the analysis of variance one way ANOVA. The result showed that the percentage of motility, viability and intact plasma membrane spermatozoa at the 5th day after diluent showed a significant differece (p<0,05) between 0,05 mg green tea extract in skim milk diluent; 0,10 mg green tea in skim milk diluent and 0,15 mg green tea extract in skim milk diluent. Motility at the 3th day after diluent respectively 46,67b ± 2,582; 43,33bc ± 4,028; 40,00ab ± 3,162; and 37,50a ± 2,739. Viability at the 3th day after diluent respectively are 79,50b ± 1,643; 77,67b ± 3,327; 77,00ab ± 3,230; and 73,83a ± 3,061. Intact plasma membrane at the 3th day after diluent respectively are 50,17b ± 2,483; 47,00ab ± 3,899; 46,83ab ± 2,858; and 46,00a ± 3,286. The conclusion of this study was the green tea extract of 0,05 mg in milk skim diluent could increase the percentage of sperm quality until 5th day after diluent.

Co-Authors Arimbi Dian Ayu Permatasari Dilla Aranda Mayola ERMA SAFITRI Hana Eliyani Indah Norma Triana Maulinda Agchirilia Vladika Mirza Asafaizh Arini Moch. Rizky Darmawan Poedji Hastutiek Rimayanti Rimayanti Ririn Ririn Rochmah Kurnijasanti Suherni Susilowati Suzanita Utama Tatik Hernawati Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito

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