Claim Missing Document
Check
Articles

Found 22 Documents
Search

The effect of active compound isolated from the leaves of kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line Mardihusodo, Hajid Rahmadianto; Hartati Wahyuningsih, Mae Sri; Astuti, Indwiani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.662 KB) | DOI: 10.19106/JMedScie004503201301

Abstract

Colorectal cancer is the tenth most common form of malignant tumor of hospital inpatients inIndonesia. Advance approaches in anticancer development is discovery molecular-targeted drugs.Molecular targets for anticancer drug have been identified including genes associated with cellcycle control and angiogenesis. Previously, an active and selective compound against WiDr fromTithonia diversifolia (Hemsley) A. has been isolated. The aim of this study was to evaluate theeffect of the isolated active compound fromT. diversifolia on the WiDr cell cycle and angiogenesis.Isolation of the active compound was performed by preparative thin layer chromatography (TLC)method. WiDr cell cycle was analyzed by flowcytometry using propidium iodide (PI).Antiangiogenesis effect was evaluated by immunocytochemistry method using anti-human VEGFmonoclonal antibody. The results showed that the effect of the isolated active compound onthe WiDr cell cycle depended on the concentration and the incubation time periods. Atconcentration of 4 μg/mL, it inhibited the WiDr cell cycle SubG1 phase after 36 and 48 hoursincubation and G1 phase after 72 hours incubation. While at concentration of 8 μg/mL, it clearlyinhibited the WiDr cell cycle G1 phase after 36, 48 and 72 hours incubation. Furthermore, theisolated active compound at concentration of 4 μg/mL significantly inhibited the VEGF expressionuntil 47.38% compared to control. In conclusion, the isolated active compound fromT. diversifoliainhibited cell cycle and angiogenesis of WiDr cell.
Peningkatan Jumlah Mikronukleus pada Mukosa Gingiva Kelinci Setelah Paparan Radiografi Panoramik Shantiningsih, Rurie Ratna; Suwaldi, Suwaldi; Astuti, Indwiani; Mudjosemedi, Munakhir
Majalah Kedokteran Gigi Indonesia Vol 20, No 2 (2013)
Publisher : Majalah Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mikronukleus merupakan salah satu tanda awal terjadinya kerusakan DNA yang ditemukan pada mukosa gingiva manusia setelah paparan radiografi dental panoramik.   Peningkatan jumlah mikronukleus terjadi paling tinggi pada hari ke-10 dan selanjutnya mengalami penurunan sampai dengan hari ke-14. Kelinci memiliki karakter dan periode turn-over mukosa gingiva yang hampir sama dengan manusia berkisar antara 10-12 hari. Tujuan penelitian ini untuk mengevaluasi apakah peningkatan jumlah mikronukleus pada mukosa gingiva kelinci setelah paparan radiografi panoramik.Sembilan ekor kelinci dibagi menjadi 3 kelompok untuk mewakili hari ke-3, 6 dan 9 setelah paparan radiografi panoramik. Sebelum dan sesudah diberikan paparan radiografi panoramik,  setiap  hewan  coba  dilakukan  apusan  pada  mukosa  gingiva  anterior  rahang  bawah  menggunakan cervical brush. Hasil apusan dilakukan pewarnaan dengan modifikasi Feulgen-Rossenbeck dan dihitung jumlah mikronukleus menggunakan mikroskop yang disambungkan dengan optilab. Analisis statistik dilakukan menggunakan paired t-test. Analisis statistik menunjukkan adanya perbedaan yang signifikan (p<0,05) antara jumlah mikronukleus sebelum dan 9 hari sesudah paparan radiografi panoramik. Akan tetapi tidak ditemukan perbedaan yang signifikan (p>0,05) antara sebelum paparan dibandingkan hari ke-3 dan ke-6 setelah paparan radiografi panoramik. Kesimpulang dari hasil penelitian ini sejalan dengan penelitian sebelumnya pada manusia bahwa peningkatan jumlah mikronukleus terjadi pada hari ke-9 setelah paparan radiografi panoramik. Hasil ini mengindikasikan bahwa pada kelinci juga menunjukkan peningkatan jumlah mikronukleus di mukosa gingiva akibat paparan radiografi panoramik.ABSTRACT: Micronucleus Increase After Panoramic Radiography Exposure In Rabbit’s Gingival Mucosa. Micronucleus is one of the early states of DNA damage found in human gingival mucosa after dental panoramic radiography exposure. The increasing amount of micronucleus will reach a peak in the tenth day after the exposure, and it will continuously decrease right after the fourteenth day. Rabbit has almost the same gingival mucosa and turn-over period with human for about 10-12 days. The purpose of this research is to evaluate the increasing amount of micronucleus in rabbit’s gingival mucosa after panoramic radiography exposure. A total of nine New Zealand rabbits were divided into 3 groups to represent day of 3rd, 6th  and 9th after the panoramic radiography exposure. The mandibular anterior gingival mucosa of each animals was swabbed using a cervical brush before and after panoramic radiography exposure. The samples were stained with Feulgen-Rossenbeck modification, and the amount of micronucleus was counted using a microscope that is connected to Optilab. Statistical analysis was performed using paired t-test. The statistical analysis showed that there was significant difference (p <0.05) between the number of micronucleus before exposure and 9th day after panoramic radiography exposure. Moreover, there was no significant difference (p> 0.05) between the amount of micronucleus before exposure compared with 3rd  and 6th  day after panoramic radiography exposure. Based on the experiment, it is concluded that the result is consistent with previous studies conducted in human that there was increasing amount of micronucleus at the 9th  day after panoramic radiography exposure. This result   indicates that rabbit   performs the increasing amount of micronucleus in gingival mucosa because of panoramic radiography exposure
CYP3A4*1G gene Polymorphism on Javanese People Sutrisna, Em; Dwiprahasto, Iwan; Astuti, Indwiani; Kristin, Erna
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.035 KB)

Abstract

AbtractMost of drugs are metabolized by cytochrome P 450 (CYP) enzyme. Cytochrome P450 3A4 is the cytochrome that is involved in metabolizing more than 60% of all medicine used in human. The variation of this CYP3A4 gene will affect the catalytic activity of this enzyme. Recently, CYP3A4*1G in intron 10 was found in Chinese and Japanese population. There is a substitution of G to A at position 82266 in intron 10. The purpose of this research was to investigate the frequency of allele and genotype CYP3A4*1G. Samples were taken from bloods of the subjects of the research. The examination of CYP3A4*1G was conducted by RTLP-PCR method.As the results of this research, the frequency of CYP3A4*1G in Javanese people is CYP3A4*1/*1 0.25, CYP3A4*1/*1G 0.55 and CYP3A4*1G/*1G 0.20. Frequency of allele G: 0.53, allele A: 0.47. The Fisher’s exact- test shows that the allele and genotype frequencyis p. 1.000. The allele and genotype frequency of Javanese people isstill in Hardy-Weinberg equilibrium.Keywords : CYP3A4*1G gene, polymorphism, Javanese people
Epidemiologic profile of oral squamous cell carcinoma in Yogyakarta, Indonesia Gracia, Isadora; Utoro, Totok; S., Supriatno; Astuti, Indwiani; Heriyanto, Didik Setyo; Pramono, Dibyo
Padjadjaran Journal of Dentistry Vol 29, No 1 (2017): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.633 KB) | DOI: 10.24198/pjd.vol29no1.11614

Abstract

ABSTRACTIntroduction: Oral cancer is a major health problem in the world, indicated by a high incidence of recurrence. In Southeast Asia, oral cancer ranks sixth from all cancers on the human body. The incidence of oral cancer, especially squamous cell carcinoma types, increases every year, but the prognosis and patients life expectancy are still unsatisfying enough and the reports about the profile of oral cancer is still limited.The objective of this research was to get the epidemiological profile of oral squamous cell carcinoma (OSCC) in Yogyakarta, Indonesia. Methods: The research method was observational analytic with cross-sectional design. All new OSCC cases in three anatomic pathology laboratory installations in Yogyakarta (January 2011-December 2015) were included in this study. Data collected were gender, age, anatomical location, and histology. All data were analyzed using statistical analysis program. Results: OSCC new cases amount increasing each year: 6 cases (6.6%) in 2011, and increased to 24 cases (26.4%) in 2015. Fifty-two of the 91 cases (57.1%) were found on male. The age group with the most cases was found in group of 41-60 years (49.5%). There is no difference in the case distribution by age between male and female. Forty-eight cases were found in the lingual part (52.7%). Histological examination showed 68 cases (74.7%) were well-differentiated carcinoma. Conclusion: OSCC profile in Yogyakarta mostly found in men with anatomical location in the lingual part in the age group above 40 years of age and were having well-differentiated histology.Keywords: Oral squamous cell carcinoma (OSCC), Epidemiologic profile, Yogyakarta 
Efek asupan vitamin D terhadap tekanan darah sistolik tikus putih (Rattus norvegicus) Hermawan, Dessy; Kadarsih, Sri; ., Sunarti; Astuti, Indwiani; Agus, Zaenal Arifin Nang
Jurnal Gizi Klinik Indonesia Vol 9, No 1 (2012): Juli
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.68 KB) | DOI: 10.22146/ijcn.15363

Abstract

Background: Hypertension still became potential life threatening disease for human life. The prevalence of hypertension was high and tends to increase. Previous study suggested that there was a linier rise in blood pressure at increasing distances from the equator. More recent study indicated that vitamin D insuffi ciency may be associated with elevated blood pressure.Objective: To investigate the effect of vitamin D intake on blood pressure.Method: This was an experimental study with pre-post test group design. The subjects were 20 male Rattus norvegicus aged 8 weeks. Subjects were divided into 5 groups and each groups received treatment as follow: lived in dark cage and consumed vitamin D free diet for 1 – 13 days, then moved to normal cage (dark-light period) and given vitamin D per-oral of 0.25 μg/kg BW for 1 – 5 days. Data of systolic blood pressure was collected and analyzed with paired t test.Results: The absence of vitamin D in diet caused a signifi cant increase in systolic blood pressure (p=0.01) after 7 days of treatment in dark cage. While oral vitamin D supplementation of 0.25 μg/kg BW caused a signifi cant decline of systolic blood pressure since the fi rst day of vitamin D supplementation and the result appeared stronger after 4 days of treatment (p=0.001).Conclusion: Vitamin D intake had a strong infl uence on systolic blood pressure
UJI EFEK HAMBATAN ATORVASTATIN TERHADAP PEMBENTUKAN SKAR HIPERTROFI PADA KELINCI NEW ZEALAND (Kajian terhadap morfologi klinis ketinggian jaringan skar, Scar Elevation Index, kepadatan kolagen, dan ekspresi VEGF) Rosyidah, Devi Usdiana; Astuti, Indwiani; Widyarini, Sitarina
Biomedika Vol 10, No 1 (2018): Biomedika Februari 2018
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v10i1.5848

Abstract

Skar hipertrofi dan keloid bagian dari jaringan parut patologis yang insiden kejadiannya masih tinggi. Keduanya mengganggu secara estetik, fisiologik, psikologik, dan sering tumbuh kembali setelah pengobatan. Belum ada terapi standar baku emas untuk skar hipertrofi dan keloid. Efek pleiotropik statin berpotensi sebagai anti skar baru. Penelitian ini bertujuan untuk mengetahui efek hambatan pembentukan skar hipertrofi dengan pemberian salep atorvastatin pada model luka skar di telinga kelinci New Zealand. Kelinci New Zealand usia 4-6 bulan (n=29) dilakukan randomisasi kedalam lima kelompok. Setelah aklimatisasi 1 minggu, dilakukan pembuatan luka model skar hipertrofi pada kedua telinga kelinci dengan punch biopsy (d=8 mm). Jumlah luka 2 buah di masing- masing telinga kanan dan kiri. Pada hari ke-7 setelah punch biopsy, kelompok I, II, III dioles salep atorvastatin dengan konsentrasi masing-masing 5%, 10%, dan 20%. Kelompok IV dioles salep clobetasol propionate 0,05% sebagai kontrol positif, dan kelompok V dioles dengan basis salep (lanolin vaselin 1:1) sebagai kontrol negatif. Frekuensi pengolesan salep pada luka ditelinga kelinci tersebut 1 kali/hari, dengan lama pengolesan selama 50 hari kedepan. Setiap hari dilakukan pengukuran morfologi klinis ketinggian skar. Pada hari ke 56 setelah punch biopsy, dilakukan penilaian akhir morfologi klinis jaringan skar hipertrofi, kemudian kelinci didekapitasi, diambil jaringan skar hipertrofi kemudian dibuat preparat histologis (HE, Von Gieson) dan imunohistokimia (VEGF). Salep atorvastatin 5% mampu menghambat pembentukan skar hipertrofi pada telinga kelinci New Zealand dengan nilai median skoring ketinggian jaringan skar sebesar 1(1-3), nilai SEI rata-rata sebesar 1,49±0,37 mm, dan nilai ekspresi VEGF sebesar 12,71±4,27%. Salep atorvastatin 5% mempunyai potensi untuk dikembangkan sebagai obat penghambat pembentukan skar hipertrofi baru dengan mekanisme penghambatan terhadap VEGF. Untuk pengembangan obat ini diperlukan penelitian lebih lanjut mengenai kapan waktu yang paling tepat mulai dioleskan salep atorvastatin setelah terjadi luka berisiko terbentuknya skar hipertrofi. Kata kunci: Atorvastatin, skar hipertrofi, VEGF, telinga kelinci.
Dust Exposures, IgE Levels, History of Allergy, and Symptoms of Allergy in Poultry Workers Wijayanti, Yuni; Sutomo, Adi Heru; Astuti, Indwiani; Asmara, Widya
KEMAS: Jurnal Kesehatan Masyarakat Vol 14, No 1 (2018)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v14i1.10232

Abstract

Poultry dust exposure may increase workers’ health risks, particularly in the form of respiratory allergic reactions. This study aimed to identify mold content of the dust and to analyze the association between dust exposure, IgE level, history of allergy, and symptoms of allergy. This study used cross sectional design with total samples of 33 workers. The data were analyzed using chi-square test and multivariate logistic regression. This study found 93.33% growth of Aspergillus sp., 69.7% work duration > 3 years, 84.8% high IgE levels, 18.2% history of allergy, and 69.7% symptoms of allergy. Dust exposure and history of allergy did not show significant correlation with symptoms of allergy while IgE levels had significant correlation with p-value of 0.036. Workers with high IgE-level were 15.986 times more likely to have symptoms of allergy (p-value 0.028). Aspergillus sp. as dust allergen potentially increased IgE levels and might become the base for facilitation of early and independent preventive and promotive efforts of workers’ health.
Photodynamic therapy (PDT) pada penderita karsinoma nasofaring: kajian angka harapan hidup Indrasari, Sagung Rai; Hariwiyanto, Bambang; Astuti, Indwiani; Sastrowijoto, Soenarto
Oto Rhino Laryngologica Indonesiana Vol 46, No 1 (2016): Volume 46, No. 1 January - June 2016
Publisher : PERHATI-KL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4753.572 KB) | DOI: 10.32637/orli.v46i1.145

Abstract

Latar belakang: Terapi karsinoma nasofaring (KNF) yang adekuat dan efektif tidak selalu tercapaidi negara berkembang. Ketersediaan alat radioterapi yang tidak seimbang dengan jumlah penderita KNFmenyebabkan keterlambatan jadwal terapi, sehingga berakibat pada buruknya hasil terapi. Alternatifterapi lain perlu dikembangkan untuk mengatasi permasalahan tersebut. Photodynamic therapy (PDT)telah digunakan untuk terapi tambahan pada berbagai kanker, termasuk KNF residu maupun rekuren.Tujuan: Mengetahui respon terapi lokal dan angka harapan hidup 5 tahun penderita KNF residu ataurekuren yang mendapatkan PDT.Metode: Kohort retrospektif dengan data rekam medis penderita KNFresidu atau rekuren yang mendapatkan PDT di Departemen THT-KL RSUP Dr. Sardjito Yogyakarta tahun2005–2011.Hasil: Local response rate sebesar 83,9% dan angka harapan hidup 5 tahun penderita sebesar60,7%. Angka harapan hidup 5 tahun penderita laki-laki lebih tinggi (73,5%) dibandingkan penderitaperempuan (42,2%), perbedaan ini bermakna secara statistik (p=0,045). Angka harapan hidup 5 tahunpenderita berusia <40 tahun lebih tinggi (76,2%) dibandingkan penderita berusia > 40 tahun (53,0%),meskipun tidak signifikan (p=0,417). Angka harapan hidup penderita yang masih terdapat tumor padanasofaring lebih baik (80%) dibandingkan penderita dengan hasil biopsi nasofaring negatif (57,8%),namun tidak signifikan (p=0,638). Angka harapan hidup penderita berdasarkan stadium saat ditegakkandiagnosis KNF, penderita stadium awal mempunyai angka harapan hidup lebih kecil (53,3%) dibandingkanpenderita stadium lanjut (62,1%), meskipun tidak signifikan (p=0,521).Kesimpulan: Local responserate PDT 83,9% dan angka harapan hidup 5 tahun sebesar 60,7%. PDT merupakan modalitas terapi yangefektif untuk KNF residu maupun rekuren. Kata kunci: Karsinoma nasofaring, photodynamic therapy, angka harapan hidup ABSTRACTBackground: Adequate and effective nasopharyngeal carcinoma (NPC) treatment cannot bereached in developing countries. The lack of radiotherapy apparatus cause delayed treatment thatbring about bad treatment results. An alternative treatment modality should be created to overcome theproblem. Photodynamic therapy (PDT) has been conducted for many malignancies including recurrentor residual NPC. Purpose: To find out the local response rate and 5-year overall survival amongrecurrent or residual NPC patients who got PDT. Method: Retrospective cohort, data was taken frommedical records of patients with PDT in ENT-H&N Surgery Department Sardjito Hospital since 2005until 2011. Results: Local response rate was 83.9%. Five-year overall survival was 6.7%. The 5-yearoverall survival among men was significantly higher than women (73.5%:42.2%) p=0.045. The 5-yearoverall survival among patients <40 years old was higher than patients > 40 years old (76.2%:53.0%)even though it was not significant (p=0.417). The 5-year overall survival among patients with tumor washigher than patients without tumor (80%:57.8%) although it was not significant (p=0.638). The 5-yearoverall survival among patients with previous early stage NPC was lower than previous advanced stage (53.3%:62.1%) although it was not significant (p=0.521). Conclusion: Local response rate of PDT was83.9% and the 5-year overall survival was 60.7%. PDT was found as an effective treatment modalityfor recurrent or residual NPC. Keywords: Nasopharyngeal carcinoma, photodynamic therapy, 5-year overall survival
Pathogenesis, evaluation, and recent management of diabetic foot ulcer Wijaya, Lorettha; Budiyanto, Arief; Astuti, Indwiani; Mustofa, .
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.122 KB) | DOI: 10.19106/JMedSci005101201910

Abstract

Diabetic foot ulcers (DFUs) are a major health problem as the number of patients continues to increase, are difficult to heal, require enormous management costs, and deteriorate the quality of life of patients, their families and societies. The pathogenesis of DFUs is complex. Most important factors that increase the risk of DFUs are peripheral neuropathy, foot deformities, frequent minor trauma, and peripheral arterial disease. Neuropeptides derangement, hypoxia, hyperglycemia, and infection act as the cause of chronicity of DFUs. Therefore, during the initial evaluation of DFU, patients need to be checked for their metabolic status, presence or absence of peripheral neuropathy, vascular insufficiency, foot deformities, and infection of the ulcer and its underlying bone. Then, DFUs are classified by the severity of vascular insufficiency, the depth of the wound, and the severity of the infection. This classification system helps clinicians to determine whether the patient needs to be hospitalized or amputated and helps to establish DFU management strategies. In the management of DFUs, adequate blood flow to the wound area should be achieved. Glycemic control and standard wound care should be encouraged. Standard wound care includes debridement, offloading, wound moisture balance with suitable dressing, edema control, and infection control. Education about preventive foot care should be taught to the patients and their families. As the pathogenesis and management of DFUs are complex, a multidisciplinary team consists of expert individuals in their respective fields should be involved.
Pengaruh Vitamin D terhadap Ekspresi COX-2, Kadar cAMP, Kadar Renin Darah dan Tekanan Darah Sistolik dalam Sistem Renin Angiotensin Aldosteron Hermawan, Dessy -; Soejono, Sri Kadarsih; Sunarti, '; Astuti, Indwiani; Nang Agus, Zainal Arifin
Jurnal Kedokteran Brawijaya Vol 29, No. 2 (2016)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2016.029.02.5

Abstract

Banyak penelitian yang melaporkan hubungan antara kadar vitamin D dalam darah dengan hipertensi, hanya saja mekanismenya dalam sistem renin angiotensin belum dapat dijelaskan dengan pasti. Hal ini yang menarik untuk mempelajari mekanisme aksi vitamin D dalam dalam pengaturan tekanan darah di sistem renin angiotensin aldosteron. Rancangan penelitian ini adalah eksperimen laboratorik dengan pre-post test group design, yang di bagi menjadi dua tahap. Tahap pertama untuk mempelajari pengaruh vitamin D terhadap ekspresi COX-2 di sel macula densa ginjal dan tahap kedua untuk mempelajari mekanisme aksi vitamin D dalam pengaturan tekanan darah. Hasil yang di dapat bahwa sasaran aksi vitamin D pada sistem renin angiotensin aldosteron kemungkinan ada di dua bagian, yaitu ada yang di bagian up-stream cAMP dengan menekan ekspresi COX-2 di sel macula densa ginjal dan ada yang di bagian down-stream cAMP, yaitu dengan menekan langsung gen yang menyandi renin. Hal ini didukung dengan data bahwa tidak terjadinya akumulasi cAMP, peningkatan kadar renin dan TDS walaupun hewan coba telah diberi teofilin bersamaan dengan vitamin D.Kata Kunci: hipertensi, vitamin D, renin, cAMP, COX-2 dan tekanan darah sistolik