Ludji Pantja Astuti
Department Of Plant Pest And Disease, Study Program Of Agroecotechnology, Faculty Of Agriculture, University Of Brawijaya Jln. Veteran, Malang, East Java 65145

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BIOLOGICAL STUDY OF TWO SPOTTED SPIDER MITE Tetranychus sp. (ACARI: TETRANYCHIDAE) ON THREE LEAF PHASES OF MUNG BEAN AND ADZUKI BEAN FOR MITE MASS REARING Puspitarini, Retno Dyah; Satria, Arif Danu; Astuti, Ludji Pantja
AGRIVITA, Journal of Agricultural Science Vol 35, No 3 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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Abstract

Two-spotted spider mite (TSM) Tetranychus sp. is one of prey mites for phyotoseiid mite mass rearing.  Based on previous research that TSM population developed well on Mung bean (Vigna radiata) and Adzuki bean (V. angularis) as host plants, confirmed growth and development of TSM on both beans. Research was aimed to observe biology of TSM on three phases of Mung bean and Adzuki bean leaf such as at primary leaf unrolled completely (V1), at the 1st trifoliate unrolled completely (V2), and at the 2nd trifoliate unrolled completely (V3). Pre adult stadia, life cycle, male and female longevity, female fecundity of TSM, and trichome density were observed on petri dishes contained each leaf phase of beans.  Result showed that three leaf phases of beans did not influence significantly in all biological variables, except fecundity. TSM fecundity was the highest on V3 phase of Mung bean (86.90). Trichome density on Mung bean leaf surface per mm² was lower than Adzuki bean. Trichome density of upper leaf surface of V3 phase of Mung bean (10.53) and Adzuki bean  (3.07) were lower than V1 (13.20; 12.40) and V2 phase (6.20; 5.27). V3 phase of Mung bean was most suitable for TSM mass rearing.Key words:  fecundity, leaf phase, mass rearing, trichome density, two-spotted spider mite   
Susceptibility of Four Rice Types to Sitophilus oryzae Linnaeus (Coleoptera: Curculionidae) Astuti, Ludji Pantja
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.2252

Abstract

The stored rice is often damaged by storage pests. As an important storage pest, Sitophilus oryzae causes significant weight loss on stored rice. The objective of this research was to evaluate the susceptibility of four rice types on Sitophilus oryzae. The research was conducted in Plant Pest Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya from May to September 2017. There were four rice types i.e. Brown rice (Diamond variety), Milled rice (Ciherang variety), Black rice (Watudodol variety), and Red rice (BWI-1 variety). Dobie’s Index of Susceptibility was adopted to categorize each rice type. In addition, the Analysis of Variance and Tukey HSD were used to compare the differences between four rice types at α < 0.05. Based on the Susceptibility Index, milled rice was categorized as rice resistant to S. oryzae, while black, brown, and red rice were moderate resistant. The resistance of rice varieties to S. oryzae is influenced by feed quality factors i.e. the adequacy and balance of nutrients, especially ash and fat.
Collonization and Oviposition Preference of Six Weevil Species on Various Colors of Storage Container Ludji Pantja Astuti; Rommy Parcelino Prabowo; Akhmad Rizali; Mutala&#039;liah Mutala&#039;liah
Jurnal Perlindungan Tanaman Indonesia Vol 23, No 2 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.40558

Abstract

Color preferences on post-harvest adult pests are useful for monitoring instrument development which based on the colonization and oviposition behavior. The research was aimed to determine the most attractive color with a certain wavelength for the test insects. The research was conducted in laboratory conditions of Plant Pest Laboratory, Department of Plant Pest and Disease, Faculty of Agriculture, University of Brawijaya at 27 ± 2°C, RH 65 ± 5%; and day:light 12:12 hours. Study of color preferences on six species of weevil used free choice test method and set by CRD with four replications using eight colors: white, red, orange, yellow, green, blue, indigo, and purple. The observed parameters were the number of adults and eggs laid. Results showed that each post-harvest insect had a different preference for various colors in the range of 380–450 nm wavelength. Adults of Sitophilus oryzae, Oryzaephilus surinamensis, O.s Mercator, and Tribolium castaneum were more preferred to feed and lay eggs in blue color (451–495 nm), Lasioderma serricorne was more preferred in indigo color (445–450 nm), and S. zeamais was more preferred in purple color (380–444 nm). The oviposition preference revealed that there was a positive correlation between the number of females and the egg laid.
Susceptibility of Maize Genotypes to Maize Weevil Sitophilus zeamais Motsch. (Coleoptera: Curculionidae) Bambang Tri Rahardjo; Ludji Pantja Astuti; Arifin Noor Sugiarto; Akhmad Rizali
AGRIVITA, Journal of Agricultural Science Vol 39, No 3 (2017): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i3.1278

Abstract

One possibility to protect the maize from storage pests is by developing genotypes that resistant to maize weevil (Sitophilus zeamais Motsch). The research was aimed to evaluate the susceptibility of several maize genotypes including local variety to the maize weevil. Six genotypes i.e. SJA, G10-12-20, G10-1-3, G10-1-7, G10-1-20 and G10-1-17 and Tosari as local variety were used. No-choice assay under laboratory condition with observed variables was laid an egg and F1 progeny number, the median time of development and weight loss of infested kernel. The susceptibility index was assessed using Dobie’s susceptibility index. Results showed that the number of eggs laid by five females and infested samples weight loss were not significantly different between six maize genotypes as well as local variety. In contrast, the number of F1 progeny emerged was significantly different. Based on the susceptibility index, the maize genotype of G10-1-3 and G10-1-17 were resistant to the weevil. While other maize genotypes and local variety were considered as moderate resistant. Based on analysis of chemical and physical characteristic revealed that maize genotypes with higher of the total phenolic compound and physical hardness tend to resist from weevil damaged.
BIOLOGICAL STUDY OF TWO SPOTTED SPIDER MITE Tetranychus sp. (ACARI: TETRANYCHIDAE) ON THREE LEAF PHASES OF MUNG BEAN AND ADZUKI BEAN FOR MITE MASS REARING Retno Dyah Puspitarini; Arif Danu Satria; Ludji Pantja Astuti
AGRIVITA, Journal of Agricultural Science Vol 35, No 3 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i3.252

Abstract

Two-spotted spider mite (TSM) Tetranychus sp. is one of prey mites for phyotoseiid mite mass rearing.  Based on previous research that TSM population developed well on Mung bean (Vigna radiata) and Adzuki bean (V. angularis) as host plants, confirmed growth and development of TSM on both beans. Research was aimed to observe biology of TSM on three phases of Mung bean and Adzuki bean leaf such as at primary leaf unrolled completely (V1), at the 1st trifoliate unrolled completely (V2), and at the 2nd trifoliate unrolled completely (V3). Pre adult stadia, life cycle, male and female longevity, female fecundity of TSM, and trichome density were observed on petri dishes contained each leaf phase of beans.  Result showed that three leaf phases of beans did not influence significantly in all biological variables, except fecundity. TSM fecundity was the highest on V3 phase of Mung bean (86.90). Trichome density on Mung bean leaf surface per mm² was lower than Adzuki bean. Trichome density of upper leaf surface of V3 phase of Mung bean (10.53) and Adzuki bean  (3.07) were lower than V1 (13.20; 12.40) and V2 phase (6.20; 5.27). V3 phase of Mung bean was most suitable for TSM mass rearing.Key words:  fecundity, leaf phase, mass rearing, trichome density, two-spotted spider mite   
Feeding Preference and Development of Sitophilus oryzae L. on Organic and Inorganic Rice Cultivation Ludji Pantja Astuti
AGRIVITA, Journal of Agricultural Science Vol 41, No 3 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i3.2362

Abstract

This study examined the preferences and development of Sitophilus oryzae on different organic and inorganic rice varieties by free-choice and no-choice tests. Feeding preference was assessed by the male and female adult present and the percentage of weight loss. The development was assessed by the numbers of eggs, larvae, pupae, and adults, the period of eggs, larvae, and pupae, the total development time, larval growth index, growth index, and biotic potential. The free-choice test showed that the number of male and female present and the percentage of weight loss were higher on inorganic rice than on organic rice of either the Shinta Nur or IR-64 variety. The no-choice test showed that the numbers of eggs, larvae, pupae, and adults were higher on inorganic rice than on both varieties of organic rice. The period of eggs, larvae, and pupae and the total development time were shorter on inorganic rice than on organic rice. The larval growth index, growth index, and biotic potential were higher on inorganic rice than on organic varieties. Organic rice had high levels of phenolic, amylose, and carbohydrates, but smaller seed size and lower protein levels and ash was preferred less compared with inorganic rice of both varieties.
Host preference of Tribolium castaneum (Herbst) on six kinds of flour Ludji Pantja Astuti; Mutala’liah Mutala’liah
Jurnal Entomologi Indonesia Vol 17 No 3 (2020): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.17.3.149

Abstract

The red flour beetle, Tribolium castaneum (Herbts) is a major pest of flour from various grains. The infested flour is discoloured and will emit a disagreeable odour due to the secretion of benzoquinone from the insect’s abdominal glands. Considering the economic losses effected by T. castaneum, new alternative control measures are needed for this pest. This study sought to determine the host preferences of T. castaneum from amongst six kinds of flour to assess their vulnerability to infestation. The research was conducted in the Plant Pest Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya. The host preferences of T. castaneum were examined using a six-arm olfactometer. Feeding preference tests were performed for a duration of 12 hours at 200 ml/minute airflow in each chamber-arm, followed by oviposition preference observations one week later. Feeding preference was calculated for the total pool of adults observed, segregated by sex, whereas oviposition preference was calculated as the number of eggs laid. Flour nutrition (proximate composition, phenolic content, and riboflavin content) were analyzed. Our results showed a feeding preference by T. castaneum for bran, soy and tapioca flour was greater over wheat, corn, and white gelatinous rice flour, while for oviposition, bran flour was the most preferred.
Rhyzopertha dominica Fabricius (Coleoptera: Bostrichidae): Growth and Development on Six Maize (Zea mays L.) Varieties Ludji Pantja Astuti; Surya Setyawan; Tita Widjayanti
AGRIVITA, Journal of Agricultural Science Vol 44, No 2 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i2.3716

Abstract

The research aimed to evaluate the Rhyzoperta dominica growth and development on six maize varieties. The No-choice method was adopted to evaluate the R. dominica life cylce from February to September 2018. Based on the result, the R. dominica growth and development were appropriate to be examined by the number of eggs, larvae, pupae, and F1 progenies and the percentage of weight loss rather than using the development time, the stage of development time, the median development time, the growth index of larvae, the potential of biotic, and susceptibility index. The development and the median development time were positively correlated with number of eggs, larvae, pupae, and F1 progenies. The R. dominica growth and development were faster on maize ‘ROK’ variety than other maize varieties. The highest number of eggs, larvae, pupae, and F1 progenies and the percentage of weight loss were detected on ‘ROK’ variety. On the other hand, the lowest growth and the longest development were observed on ‘Anoman’ variety. The variation of the R. dominica growth and development was due to the variation of the physical and biochemical characteristics of maize varieties.
PERKEMBANGAN Sitophilus oryzae LINNAEUS (COLEOPTERA: CURCULIONIDAE) PADA BERBAGAI JENIS PAKAN Sri Ria Vidia Antika; Ludji Pantja Astuti; Rina Rachmawati
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 2 No. 4 (2014)
Publisher : Universitas Brawijaya

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Abstract

Penelitian perkembangan S. oryzae pada beras, gandum, jagung, kacang tanah, kacang hijau, dan kedelai dilakukan di dalam laboratorium (27OC and 70% RH) menggunakan metode no choice test. Dalam penelitian ini terdapat 6 variabel pengamatan, yaitu mortalitas imago (%), jumlah imago baru yang muncul, penurunan berat pakan (%), periode perkembangan S. oryzae, berat imago baru, dan indeks kepekaan. Hasil penelitian menunjukkan bahwa S. oryzae tidak dapat berkembang pada kacang tanah, kacang hijau dan kedelai. Perkembangan S. oryzae lebih baik pada beras dibandingkan pada gandum dan jagung, hal ini dilihat dari persentase penurunan berat pada beras (11,81%) lebih besar daripada gandum (0.90%) dan jagung (3,92%). Berdasarkan nilai indeks kepekaan, beras (10.27) dan jagung (9,440) termasuk dalam kategori peka terhadap S. oryzae, sedangkan gandum (7,130) termasuk dalam kategori agak tahan.Kata kunci: perkembangan, Sitophilus oryzae, jenis pakan, indeks kepekaan.
Kepekaan Beberapa Varietas Ubi Jalar (Ipomoea batatas (L.) Lam.) terhadap Hama Cylas formicarius Fabricius (Coleoptera: Curculionidae) Septi Mauludiana; Ludji Pantja Astuti; Toto Himawan
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 3 No. 1 (2015)
Publisher : Universitas Brawijaya

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Abstract

ABSTRAK Kumbang Cylas formicarius Fabricius (Coleoptera: Curculionidae) merupakan hama utama pada ubi jalar (Ipomoea batatas (L.)Lam.). Kerusakan serius umumnya terjadi selama umbi dalam masa penyimpanan (Kalshoven, 1981).Oleh karena itu, pengujian kepekaan terhadap varietas ubi jalar perlu dilakukan sebagai acuan untuk menentukan cara penyimpanan ubi jalar yang akan dilakukan. Penelitian ini bertujuan untuk mengetahui kepekaan ubi jalar varietas Ayamurasaki, Beni Azuma, Beta 2, Bis Op-4, Sari dan Sawentar terhadap hamaC. formicarius di tempat penyimpanan.Hasil penelitian menunjukkan bahwa dewasa C. formicarius memiliki preferensi makan dan preferensi oviposisi sama pada keenam varietas ubi jalar.Selain itu, nilai indeks kepekaan juga menunjukkan bahwa semua varietas ubi jalar yang diuji agak peka terhadap seranganC. formicarius di tempat penyimpanan. Kata kunci: Cylas formicarius, varietas ubi jalar, indeks kepekaan