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All Journal Jurnal Kedokteran Brawijaya Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Mutiara Medika: Jurnal Kedokteran dan Kesehatan
Soedjono Aswin
Bagian Anatomi, Embriologi dan Antropologi, Fakultas Kedokteran Universitas Gadjah Mada
Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

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Binaural entrainment of 2000-2040 Hz and 2000-2090 Hz increase Glial Fibrillary Acidic Protein (GFAP) expression of astrocytes in the CA1 rat hippocampus during operant learning conditioning Nugraha, Zainuri Sabta; Aswin, Soedjono; Harijadi, Harijadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.092 KB) | DOI: 10.19106/JMedScie004603201401

Abstract

Astrocytes of hippocampus contribute in the learning performance. Entrainment of gamma wavescan improve learning performance by improving the neurons to astrocytes communication. Theaim of this study was to evaluate the effect of binaural entrainment of 2000-2040 Hz and 2000-2090 Hz on Glial Fibrillary Acidic Protein (GFAP) expression in astrocytes of the CA1 region ofrat hippocampus during operant learning conditions. Twenty male Wistar rats aged 4-6 weekswith body weight 100-150 g were devided into 4 groups. Group I was given binaural soundsentrainment at 2000-2040 Hz without learning test. Group II was given entrainment as performedin Group I followed by a learning test. Group III was given binaural sounds entrainment at 2000-2090 followed by a learning test. Group IV was not given entrainment nor learning test. Theentrainment was performed for 30 minutes everyday for 12 days and the learning test wasperformed for 10 minutes everyday for 12 days. The GFAP expression was examinedimminohistochemically. Astrocytes processes and astrocytes histoscore were also calculated.The results showed that the number of the GFAP-positive astrocytes in Group I (70.96 ± 4.86),II (69.76±3.07) and III (63.10±5.85) were significantly higher than Control (47.33±1.33)(p<0.05). The number of the processes astrocytes in Group I (47.64 ± 3.87), II (60.66±2.07)and III (54.17±6.38) was significantly higher than Control (30.87±2.69) (p<0.05). Moreover,the number of the processes astrocytes in Group II was significantly higher than Group I (p=0.016).The astrocytes histoscore index in the Group II (115.58±14.13) and III (78.32±22.23) weresignificantly higher than Group I (28.79 ± 9.61) and Control (16.05±1.64) (p<0.05). Inconclusion, the binaural entrainment of 2000-2040 Hz and 2000-2090 Hz increase GFAP expressionof astrocytes in Cornu Ammonus 1 (CA1) region of rat hippocampus during operant learningconditioning.
Intensitas Fluoresensi Neuron-Neuron Dopaminergik Di Area Ventralis Tegmenti Setelah Pemberian Alkohol Secara Kronis Pada Tikus (Railus norvegicus) Makiyah, Sri Nabawiyati Nurul; Aswin, Soedjono; HNFS, Marsetyawan
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 5, No 1 (2005)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v5i1.1865

Abstract

The aim of this study is to reveal the effects of chronically alcohol treat-ment on the intensity of fluorescence dopaminergic neurons in the ventral teg-mental area of the rats.In this study, the rats were divided randomly into three groups (each group consists of five rats), i.e. control group (without any treatment), treatment control group (was given 2 ml aquadest intraperitoneally) and treatment group (was given 2 ml of 15 % alcohol intra-peritoneally). After the 25th day, the rats were killed by decapitation followed by taking out their brains and then were processed for immunohistochemically microscopic preparations of ventral teg-mental area.The results of this study showed that the rats, after they were treated with alcohol chronically, showed a decrease in the intensity of fluorescence dopam-inergic neurons in the ventral tegmental area compared with the control group and treatment control group . It is concluded that chronic alcohol treatment pro-duced a decrease in the intensity of fluorescence dopaminergic neurons in the ventral tegmental area.Tujuan penelitian ini adalah untuk mengungkapkan pengaruh pemberian alkohol secara kronis terhadap intensitas fluoresensi neuron dopaminergik di area ven- tralis tegmenti pada tikus.Pada penelitian ini tikus (Rattus norvegicus) dibagi menjadi 3 kelompok secara random yaitu kelompok kontrol (K), kelompok kontrol perlakuan (KP) dan kelompok perlakuan (P), tiap kelompok terdiri dari 5 ekor tikus. Selama 25 hari, kelompok K tidak diberi perlakuan apa-apa, kelompok KP diberi 2 ml akuades secara intra- peritoneal (ip), dan kelompok P diberi 2 ml alkohol 15% ip. Pada hari ke-26, semua tikus didekapitasi untuk diambil otaknya kemudian dibuat sediaan gambaran mikrokopis area ventralis tegmenti secara imunohistokimiawi.Hasil penelitian ini menunjukkan bahwa pemberian alkohol secara kronis menurunkan intensitas fluoresensi neuron dopaminergik di area ventralis tegmenti pada tikus dibandingkan dengan tikus kelompok kontrol dan kelompok kontrol perlakuan. Oleh karena itu dapat disimpulkan bahwa pemberian alkohol secara kronis menurunkan intensitas fluoresensi neuron dopaminergik di area ventralis tegmenti pada tikus.
PEMBERIAN ETANOL JANGKA PANJANG MENURUNKAN MEMORI KERJA SPASIAL PADA TIKUS Aswin, Soedjono; Mustofa, Mustofa
Jurnal Kedokteran Brawijaya Vol 24, No 2 (2008)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.933 KB) | DOI: 10.21776/ub.jkb.2008.024.02.7

Abstract

Long term ethanol administration may cause morphological changes in the hippocampus, followed by deficit in  the  hippocampal  function  including  the  impairment  of  memory.  The  aim  of  this  study  is  to  investigate  the changes of spatial working memory of rats after chronic ethanol administration at adolescent age. Twenty five adolescent  (30  days  of  age)  male  rats  (Rattus  novergicus)  divided  randomly  into  5  groups  :  control  without treatment (C1), control with treatment (C2), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3) group. Each group consist of 5 rats. C2 group was given physiologic saline, T1, T2, and T3 group were given ethanol with doses  1,  2,  and  3  g/kg/day  for  30  days  by  intraperitoneal  injection.  After  chronic  ethanol  administration  the spatial  working  memory  of  5  groups  were  tested  by  using  8  arm  radial  maze  for  12  days.  Spatial  working memory measured by total number of entered arm maze and error A type. The results of this study showed that  chronic  ethanol  administration  at  adolescent  age  caused  decreasing  of  spatial  working  memory  of  the rats. The total number of entered arms maze and error A type for treatment group (especially P3 ) is higher than  control  groups  (p<0,05).  It  is  concluded  that  in  rats,  chronic  ethanol  administration  at  adolescent  age caused decreasing spatial working memory.   Key words : Ethanol, Spatial working memory, Adolescent
Pengaruh Durasi Pemberian Ekstrak Etanol Pegagan (Centella asiatica sp.) terhadap Memori Spasial Tikus Putih (Rattus norvegicus) pascastres Listrik Sari, Dwi Cahyani Ratna; Pratama, Reza Satria; Aswin, Soedjono; Suharmi, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 2 (2011)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v11i2.932

Abstract

Beberapa penelitian telah dilakukan berkaitan dengan kemampuan pegagan sebagai neurotropik dan neuroprotektif. Tujuan penelitian ini untuk mengungkapkan pengaruh durasi pemberian ekstrak etanol pegagan dalam peningkatan memori spasial tikus putih pascastres. Pada penelitian ini, 21 tikus jantan, usia delapan minggu dibagi dalam tiga kelompok: dua kelompok perlakuan (K1 dan K2) dan satu kelompok kontrol (KN). Kelompok perlakuan menerima ekstrak ethanol pegagan sebesar 150 mg/kgBB/ ml secara oral selama empat(K1) dan enam(K2) minggu. Kelompok kontrol akan menerima aquades 1 ml selama enam minggu. Semua kelompok akan diuji memori dengan menggunakan maze radial delapan lengan selama 12 hari sebelum dan setelah perlakuan. Uji stres listrik selama 10 menit dilakukan sebelum perlakuan. Hasil penelitian menunjukkan persentase tertinggi ketepatan pemilihan lengan dalam uji maze radial 2 (UMR2) untuk KN, K1 dan K2 masing-masing sebesar 23,6%, 44,8% dan 91,71%, dengan rerata persentase masing-masing sebesar 10,24%, 14,12% dan 53,33%. Uji Mann-Whitney menunjukkan bahwa persentase ketepatan pemilihan lengan berbeda secara bermakna antara satu kelompok dengan kelompok lainnya. Kesimpulannya adalah pegagan mampu meningkatkan memori spasial tikus putih pascastres dan pemberian ekstrak etanol pegagan selama enam minggu memberikan efek peningkatan memori yang lebih signifikan dibanding pemberian selama empat minggu.
Lamanya Pemberian Ekstrak Etanol Centella asiatica sp. Meningkatkan Memori Spasial Tikus Pasca Stres Listrik Sari, Dwi Cahyani Ratna; Pratama, Reza Satria; Aswin, Soedjono; Suharmi, Sri
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 13, No 3 (2013)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v13i3.2479

Abstract

Beberapa penelitian telah dilakukan berkaitan dengan kemampuan Pegagan (Centella asiatica) sebagai neurotropik dan neuroprotektif. Tujuan penelitian ini untuk mengungkapkan pengaruh durasi pemberian ekstrak etanol C. asiatica dalam peningkatan memori spasial tikus putih pasca stres. Pada penelitian ini, 21 tikus jantan, umur delapan minggu dibagi dalam tiga kelompok: dua kelompok perlakuan (K1 dan K2) dan satu kelompok kontrol (KN). Kelompok perlakuan menerima ekstrak ethanol C. asiatica sebesar 150 mg/kgBB/ml secara oral selama empat (K1) dan enam (K2) minggu. Kelompok kontrol menerima aquades 1 ml selama enam minggu. Semua kelompok akan diuji memori dengan menggunakan maze radial delapan lengan selama 12 hari sebelum dan setelah perlakuan. Uji stres listrik selama 10 menit dilakukan sebelum perlakuan. Hasil penelitian menunjukkan persentase tertinggi ketepatan pemilih­an lengan dalam uji maze radial 2 (UMR2) untuk KN, K1 dan K2 masing-masing sebesar 23,6%, 44,8% dan 91,71%, dengan rerata persentase masing-masing sebesar 10,24%, 14,12% dan 53,33%. Uji Mann- Whitney menunjukkan bahwa persentase ketepatan pemilihan lengan berbeda secara bermakna antara satu kelompok dengan kelompok lainnya. Disimpulkan bahwa C. asiatica mampu meningkatkan memori spasial tikus putih pasca stres dan pemberian ekstrak etanol C. asiatica selama enam minggu memberikan efek peningkatan memori yang lebih signifikan dibanding pemberian selama empat minggu.Previous studies have shown the ability of Centella asiatica in enhancing memory by mean of its neurotrophic and neuroprotective effects. The objective of this study was to reveal the effect of C. asiatica ethanolic extract’s administration duration on spatial memory in rat after electric-stress induced. Eight weeks male rats (n=21) were devided randomly into three groups, i.e. two treated groups (K1 and K2) and one control group (KN). The rats were induced by 10 minutes electrical shock and given 150 mg/ kgBW oral C. asiatica ethanolic extract daily for four (K1) and six (K2) weeks. Control groups received 1 mL aquadest daily. The results showed that the performance which assessed by measuring the percent­age of correct-entered arm showed the maximum percentage on the accuracy of right-entering arm in radial arm maze test 2 (UMR2) of KN, K1 and K2 are respectively 23,6%, 44,8% and 91,71%, whereas the mean of percentage are 10,24%, 14,12% and 53,33%. Mann-Whitney test showed that there was significant difference among treated groups and control group (p 0,05). It is concluded that C. asiatica was able to enhance spatial memory and the effect is more prominent in the group with longer period of administration duration of Pegagan ethanolic extract.
Intensitas Fluoresensi Neuron-Neuron Dopaminergik Di Area Ventralis Tegmenti Setelah Pemberian Alkohol Secara Kronis Pada Tikus (Railus norvegicus) Makiyah, Sri Nabawiyati Nurul; Aswin, Soedjono; HNFS, Marsetyawan
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 5, No 1 (2005)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v5i1.1865

Abstract

The aim of this study is to reveal the effects of chronically alcohol treat-ment on the intensity of fluorescence dopaminergic neurons in the ventral teg-mental area of the rats.In this study, the rats were divided randomly into three groups (each group consists of five rats), i.e. control group (without any treatment), treatment control group (was given 2 ml aquadest intraperitoneally) and treatment group (was given 2 ml of 15 % alcohol intra-peritoneally). After the 25th day, the rats were killed by decapitation followed by taking out their brains and then were processed for immunohistochemically microscopic preparations of ventral teg-mental area.The results of this study showed that the rats, after they were treated with alcohol chronically, showed a decrease in the intensity of fluorescence dopam-inergic neurons in the ventral tegmental area compared with the control group and treatment control group . It is concluded that chronic alcohol treatment pro-duced a decrease in the intensity of fluorescence dopaminergic neurons in the ventral tegmental area.Tujuan penelitian ini adalah untuk mengungkapkan pengaruh pemberian alkohol secara kronis terhadap intensitas fluoresensi neuron dopaminergik di area ven- tralis tegmenti pada tikus.Pada penelitian ini tikus (Rattus norvegicus) dibagi menjadi 3 kelompok secara random yaitu kelompok kontrol (K), kelompok kontrol perlakuan (KP) dan kelompok perlakuan (P), tiap kelompok terdiri dari 5 ekor tikus. Selama 25 hari, kelompok K tidak diberi perlakuan apa-apa, kelompok KP diberi 2 ml akuades secara intra- peritoneal (ip), dan kelompok P diberi 2 ml alkohol 15% ip. Pada hari ke-26, semua tikus didekapitasi untuk diambil otaknya kemudian dibuat sediaan gambaran mikrokopis area ventralis tegmenti secara imunohistokimiawi.Hasil penelitian ini menunjukkan bahwa pemberian alkohol secara kronis menurunkan intensitas fluoresensi neuron dopaminergik di area ventralis tegmenti pada tikus dibandingkan dengan tikus kelompok kontrol dan kelompok kontrol perlakuan. Oleh karena itu dapat disimpulkan bahwa pemberian alkohol secara kronis menurunkan intensitas fluoresensi neuron dopaminergik di area ventralis tegmenti pada tikus.
Co-Authors Dwi Cahyani Ratna Sari Harijadi Harijadi HNFS, Marsetyawan HNFS, Marsetyawan Mustofa Mustofa Reza Satria Pratama, Reza Satria SN Nurul Makiyah Sri Suharmi, Sri Zainuri Sabta Nugraha, Zainuri Sabta