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Sukmadjaja Asyarie
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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DNA Condensation Study of Fully Synthesized Lipopeptide-Based Transfection Agent for Gene Delivery Vehicle Tarwadi, Tarwadi; Rachmawati, Heni; Kartasasmita, Rahmana E.; Pambudi, Sabar; Arbianto, Alfan Danny; Restiani, Dewi Esti; Asyarie, Sukmadjaja
ANNALES BOGORIENSES Vol 22, No 2 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.168 KB) | DOI: 10.14203/ann.bogor.2018.v22.n2.65-74

Abstract

   The main requirement of transfection agent has to condense DNA in nanoparticle size, protect the DNA from nucleases and other degrading enzymes during its transport in cell cytoplasm and nucleus and should not toxic to target cells. In this research, lipopeptide composed of palmitoyl (C-16) and short peptide sequence have been designed fully synthesized and tested to DNA condensation capability and toxicity. The DNA condensation study was performed using EtBr exclusion assay and cytotoxicity determination was carried out by colorimetric MTT assay. It was revealed that lipopeptide-based transfection agent of Pal-CKKHH and Pal-CKKHH-YGRKKRRQRRR-PKKKRKV condensed DNA molecules efficiently. The lipopeptide was less toxic compared to Lipofectamine and Poly-L-Lysine, that shown by 90% of CHO-K1 cells remained viable when they were treated with 4.36 µM Pal-CKKHHYGRKKRRQRRR-PKKKRKV. Meanwhile, there were only ~75% and 80% of CHO-K1 viable cells when it was treated with PLL and Lipofectamine®2000, respectively. Moreover, cell viability of HepG2 was ~ 75% after treated with 2.18 µM of Pal-CKKHH-YGRKKRRQRRR-PKKKRKV and decreased to ~65% when the lipopeptide concentration increased to 8.72 M. In summary, the synthesized lipopeptide condenses DNA molecules efficiently, less toxic than Lipofectamine®2000 and PLL and has possibility to be explored as a non-viral gene delivery vehicle.
STUDI PADATAN DENGAN DIFFERENTIAL SCANNING CALORIMETRY DAN SCANNING ELECTRON MICROSCOPE PADA MIKROSFER PAUTAN SILANG ALGINAT YANG MENGANDUNG METFORMIN HCL Nugrahani, Ilma; Asyarie, Sukmadjaja; Utami, Ratna A.; Putra, Okky D.
Acta Pharmaceutica Indonesia Vol 36, No 3 & 4 (2011)
Publisher : School of Pharmacy Institut Teknologi Bandung

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Abstract

Tujuan dari penelitian ini adalah mempelajari interaksi fisika antara alginat dan metformin HCl dalam bentuk mikrosfer. Interaksi fisika diamati dengan menggunakan differential scanning calorimetry (DSC) dan scanning electron microscope (SEM). Mikrosfer dengan efisiensi penjeratan optimum ditentukan jenis interaksinya dengan DSC yang didukung dengan analisis morfologi mikrosfer yang dihasilkan oleh SEM. Termogram DSC menunjukkan interaksi fisika metformin HCl-matriks alginat yang ditunjukkan dengan perubahan profil kurva endotermik pada suhu 228-230°C dan 265-35°C. Hasil SEM mikrosfer menunjukan bahwa metformin berada dalam bentuk kristal sedangkan alginat hasil pautan silang dalam bentuk amorf. Dapat disimpulkan bahwa interaksi fisika yang terjadi adalah ikatan hidrogen yang kuat antara metformin HCl dengan alginat yang berefek pada meningkatnya efisiensi penjeratan.
Identifikasi Interaksi Kimia Fisika Pada Kombinasi Antibiotika Amoksisilina Trihidrat - Kalium Klavulanat Dengan Kalorimeter Larutan Dan Rekristalisasi Nugrahani, Ilma; Asyarie, Sukmadjaja; N.S, Sundani; Ibrahim, Slamet
Majalah Ilmu Kefarmasian Vol. 4, No. 3
Publisher : UI Scholars Hub

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Abstract

Physico-chemical interaction of amoxicillin trihydrate - potassium clavulanate has been investigated. The method used in this experiment was solution calorimeter analysis that was collaborated by the re-crystallization observation. Amoxicillin-clavulanate were mixed on the variety ratios (0:10; 9:1; ...; 1:9; 0:10) and analyzed by solution calorimeter in the HCl 0,1 N and phosphate buffer pH 6,8 solution as the gastrointestinal liquids. After that, the re-crystallizations of amoxicillin-clavulanate on the variety ratios were analyzed by polarization microscope and digital camera. The results of observation showed that amoxicillin-clavulanate have the physical-chemical interactions in the ratios 3:7; 5:5; 7:3 respectively in both of pH models of gastrointestinal liquids.
Pengamatan Visual Tahap-Tahap Pembentukan Kristal Sistem Eutektikum Asetaminofen-Pseudoefedrin Hidroklorida Nugrahani, Ilma; Asyarie, Sukmadjaja; Soewandhi, Sundani Nurono; Ibrahim, Slamet
Majalah Ilmu Kefarmasian Vol. 7, No. 1
Publisher : UI Scholars Hub

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The aim of this research was to observe the recrystallization process of two component, acetaminophen-pseudoephedrine hydrochloride in ethanol and the thermal profiles. The visual data resulted from polarization microscope observation confirmed with thermograph DSC showed that the crystal habit was represented to the intrinsic character representative by thermodynamic properties of the binary system.The visualization process proved that in different molar ratios, the binary system formed crystals habit with different and step by step showed inhibit, to form polycrystalline until at molar ratio 6:4 formed the fines single crystals mixture which was smaller than before co-recrystallization. Acetaminophen was observed as monoclinic hexagonal crystals while pseudoephedrine hydrochloride was observed monoclinic/orthorombic forms.
Polimorfisasi dan Solvatomorfi Amoksisilina Trihidrat setelah Proses beku Kering Nugrahani, Ilma; Asyarie, Sukmadjaja; Soewandhi, Sundani Nurono; Ibrahim, Slamet
Majalah Ilmu Kefarmasian Vol. 7, No. 2
Publisher : UI Scholars Hub

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The polymorphismof amoxicillin has been identified after freeze drying process. The freeze dried amoxicillin have some specific physical properties different from their raw material, that was proved by DSC, XRD, and polarize microscope. Exothermal curve from DSC thermogram changes to endothermal curve, diffractogram XRD changes to different profile, and the crystal shows different habit. All of data showed that freeze drying process improve amoxicillin to different crystal form which has higher melting oxidation point and lower hydrate. The improvement of the crystal structure might be impact to change physical-pharmaceutical properties like dissolution, absorption, and change it antibiotic potency.
Co-Authors Arbianto, Alfan Danny HENI RACHMAWATI Ilma Nugrahani N.S, Sundani Pambudi, Sabar Putra, Okky D. Rahmana E. Kartasasmita Restiani, Dewi Esti Slamet Ibrahim Sundani Nurono Soewandhi Tarwadi, Tarwadi Utami, Ratna A.