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All Journal Jurnal Kedokteran Brawijaya Prosiding UNEJ e-Proceeding BIOTROPIC The Journal of Tropical Biology Molekul: Jurnal Ilmiah Kimia Scripta Biologica Jurnal Manusia dan Lingkungan
Saefuddin Aziz
Fakultas Biologi, Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Medicine & Pharmacology Social Sciences

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DETEKSI GEN NRPS AKTINOMISETES SIMBION RUMPUT LAUT DAN KARANG LUNAK , Riyanti; Aziz, Saefuddin; Sabdono, Agus; Radjasa, Ocky Karna
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Aktinomicetes merupakan bakteri Gram positif dengan kandungan GC yang tinggi dan dikenal sebagai penghasil senyawa metabolit sekunder. Namun potensi aktinomicetes laut terutama dari lingkungan laut masih sangat terbatas dikembangkan. Penelitian yang telah dilaksanakan menyatakan bahwa telah diketahui adanya asosiasi antara bakteri Gram positif yang diduga kuat adalah aktinomisetes dengan rumput laut dan softcoral. Sehingga dari hasil penelitian ini dapat dilanjutkan untuk deteksi dini gen penyandi penghasil senyawa metabolit sekunder kelas PKSs dan NRPSs dan uji aktivitasnya sebagai antioksidan. Deteksi gen PKS I dan NRPS ini dilakukan untuk mencari isolat aktinomisetes yang mempunyai potensi sistem biosintetik senyawa poliketida dan non ribosomal peptida. Sampel untuk penelitian ini diambil dari perairan Pangandaran, Jawa Barat. Sampel yang diperoleh adalah rumput laut, lamun. Metode yang digunakan dalam penelitian ini mulai dari analisa mikrobiologi (isolasi aktinomicetes, uji Gram) , analisa molekuler (rep-PCR , PCR gen NRPS). Berdasarkan penelitian ini diperoleh total aktinomicetes sebanyak 90, dengan keragaman genetik bervariasi koefisien similaritas hingga 1. Koefisien similaritas 1 menunjukkan kekerabatan genetik yang dekat dan koefisien similaritas kurang dari 1 menunjukkan kekerabatan genetik yang jauh. Hasil PCR gen NRPS diperoleh 6 isolat terdeteksi pembawa gen NRPS.
STUDI KEANEKARAGAMAN GENETIK TANAMAN KECIPIR (PSOPHOCARPUS TETRAGONOLOBUS (L.) DC): UPAYA MENUJU LABORATORIUM GENETIKA SEBAGAI PUSAT KAJIAN KETAHANAN PANGAN DAN BIOENERGI Amurwanto, Adi; Yuniaty, Alice; Susanto, Agus Hery; Sasongko, Nurtjahjo Dwi; Aziz, Saefuddin
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Kecipir mempunyai potensi untuk dimanfaatkan sebagai bahan pangan segar maupun olahan bernilai gizi tinggi dan sebagai bahan dasar dalam industri biodiesel. Pemanfaatan kecipir yang belum optimal mendorong dilakukannya penelitian tentang keanekaragaman genetik tanaman kecipir sebagai langkah awal untuk mengembangkan potensi tanaman ini. Penelitian ini bertujuan untuk memperoleh data mengenai keanekaragaman genetik pada level molekuler kecipir polong panjang dan polong pendek dengan teknik RAPD. Dari 32 primer yang diskrining, 20 dipilih untuk analisis menggunakan Program GenAlEx 6.1. Hasilnya 156 buah fragmen dengan 140 diantaranya menunjukkan polimorfisme sehingga didapatkan polimorfisme total sebesar 89,74%. Polimorfisme dalam populasi kecipir polong pendek bernilai 64.10% dan polimorfisme dalam populasi kecipir polong panjang sebesar 51.28%. Kemiripan genetik Nei (bias) antara kecipir polong panjang dan kecipir polong pendek adalah 0,805 sementara jarak genetik antara keduanya adalah 0,218. Adapun kemiripan genetik tidak bias antara kedua populasi adalah 0,873, sementara jarak genetik tidak bias sebesar 0,136.
Endothelial Nitric Oxide Synthase 3 (eNOS3) Gene Polymorphisms and Essential Hypertension in Javanese Ethnic Group Arjadi, Fitranto; Aziz, Saefuddin; Muntafiah, Alfi
Jurnal Kedokteran Brawijaya Vol 28, No 3 (2015)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (466.205 KB) | DOI: 10.21776/ub.jkb.2015.028.03.4

Abstract

Hypertension is still a major public health problem in Indonesia and in several other countries. This disease is caused by multi factorial components involving both environmental and genetic factors. eNOS3 gene is one of the enzymes related to the high prevalence of hypertension. This gene expresses the NOS enzyme which regulates the synthesis of NO. NOS enzyme causes vasodilatation which decreases peripheral resistance and lowers blood pressure. This cross sectional study compared hypertension patients to those with normal blood pressure in the age group of 40-80 years old. The main purpose of this study is to evaluate the influence of eNOS3 gene Glu298Asp allele expression in Javanese ethnic group patients with hypertension. The samples consist of 50 respondents with hypertension and 50 respondents with normotension as control. Data of eNOS3 gene polymorphisms and NO plasma levels from the respondents were analyzed using t-test and chi-square test. Glu298Asp allele genotype variation in eNOS3 gene was detected by PCR-FRLP using primers G894TF and G894TR and the PCR products were cut using Mbol restriction enzymes. Sequencing result of each polymorphism band shows a typical nucleotide sequence compared to the nucleotide sequence of eNOS3 gene in Gen Bank. The results of this study showed no connection between Glu298Asp allele polymorphism in eNOS3 gene with hypertension in Javanese. There was also no relation between eNOS3 gene polymorphisms with high levels of respondents' NO plasma. Average NO plasma level of hypertension patients is 34,53 µmol/L, whereas average NO level of normal blood pressure is 32,5 µmol/L.Keywords: Allele Glu298Asp, eNOS3 gene, G894T, hypertension, Javanese ethnic, NO plasma level 
HIGHLY SPESIFIC BACILLUS CEREUS-PHAGES ISOLATED FROM HOSPITAL WASTEWATER IN BANYUMAS REGENCY Anwar Rovik; Saefuddin ‘Aziz; Hendro Pramono
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Bacillus cereus is pathogenic bacteria that frequently contaminate food by producing entero and emetic toxins. B. cereus had shown resistance to various antibiotics. An alternative to control B. cereus is the use of bacteriophages. This study aims to determine whether bacteriophages isolated from hospital wastewater in Banyumas Regency is highly specific to B. cereus. The research was carried out descriptively through isolation, purification, enumeration, specificity, effectivity, and host-lysing rate test. B. cereus phages were isolated from hospital wastewater in Banyumas Regency with various titer, i.e. 108-109 PFU.ml-1. The PKRW-1, PKCL-1, PKSR-2, RSBMS-2, RSBMT-1, RSAJP-1, RSAJP-2, RSAJK-2, RSAJE-1, and RSAJE-3 phages have a great specificity and effectivity of infection to B. cereus. Total of 7 isolated phages have a fast host-lysing rate by the 2nd hour of incubation.
ISOLASI Rhizopus oligosporus PADA BEBERAPA INOKULUM TEMPE DI KABUPATEN BANYUMAS Ratna Stia Dewi; Saefuddin Aziz
Molekul Vol 6, No 2 (2011)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.557 KB) | DOI: 10.20884/1.jm.2011.6.2.97

Abstract

Tempe merupakan makanan tradisional asli Indonesia. Tempe mengandung gizi yang cukup tinggi, mencakup 25% protein, 5% lemak, 4% karbohidrat serta kaya akan mineral dan vitamin B12. Sentra produksi tempe di Indonesia paling banyak di temui di Jawa Tengah, dan salah satunya ada di Kabupaten Banyumas. Tekstur tempe yang kompak dan berwarna putih tercipta dari hasil kerja jamur genus Rhizopus, yang ditambahkan sebagai inokulum pada saat pembuatan tempe. Salah satu jenis jamur yang sering dijumpai dalam ragi tempe adalah Rhizopus oligosporus. Penelitian ini bertujuan untuk mengisolasi jamur R. oligosporus dari beberapa inokulum tempe di Kabupaten Banyumas. 56 jamur diisolasi dari inokulum tempe. Semua isolat termasuk dalam genera Rhizopus. 19 diantaranya dipilih berdasarkan warna konidia, yang terdiri dari isolat dengan konidia coklat keabu-abuan, dan abu-abu. Pengamatan makroskopik dan mikroskopik menunjukkan bahwa isolat terpilih adalah R. oligosporus.
DETEKSI KERAGAMAN SPESIES BAKTERI METANOGEN RUMEN SAPI MENGGUNAKAN KLONING GEN 16S rRNA DAN SEKUENSING Shoffiana Noor; Hendro Pramono; Saefuddin Aziz
Scripta Biologica Vol 1, No 4 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.925 KB) | DOI: 10.20884/1.sb.2014.1.4.43

Abstract

Ruminants produce methane gas which contributes to enhanced greenhouse effect in the atmosphere. Cattle issued the highest methane during the fermentation of feed in the rumen. Methane gas produced by methanogen bacteria in carbohydrates anaerobic fermentation. Methanogen bacteria are difficult to obtain diversity information because difficult cultured. One technique can be used is molecular rRNA 16S gene cloning and sequencing. This study was aims to determine the species diversity of methanogen bacteria in cattle's rumen using rRNA 16S gene cloning and sequencing technique by survey method. The results obtained 51 clones with 800 bp insert size length. The sequencing resulted 2 different sequences, ie 8-3L21 clone bacterium uncultured and BBS-12 clone methanogens rumen uncultured rRNA 16S gene partial sequence with 99% and 100% similarity. The Genus sequences for gene 1 and 3 were Prevotella (24%), Clostridium (1.5%), and other uncultured bacteria, whereas the 2 gene sequences of species was Methanobrevibacter ruminantium (21.83%), M. millerae (29.17%), M. gottschalkii (6.47%), Methanosphaera stadtmanae, and Methanobacterium alcaliphilum. This research provides scientific information about cattle rumen methanogen bacteria species diversity which can be used as a basis for control of cattle rumen methanogen bacteria. 
Keragaman Morfologi Bakteri Nitrifikasi Asal Kompos Kotoran Domba pada Peternakan Domba dengan Sistem Bedding Sri Martina Wiraswati; Hendro Pramono; Oedjijono; Dini Ryandini; Dyah Fitri Kusharyati; Meyta Pratiwi; Taruna Dwi Satwika; Dwiana Muflihah Yulianti; Saefuddin Aziz; Daniel Joko Wahyono; Rizal Khoirun Alfisah; Yuriza Eshananda; Budi Rustomo; Afifah Mariana
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1695

Abstract

The application of compost bedding system on sheep farm is a technology to minimize the negative impact of sheep manure waste toward environment. The microorganisms which inhabit the compost bedding, especially nitrifying bacteria are expected to have significant role in odor emission reduction which become environmental problem around the sheep farm. This study aims to analyze the abundance and diversity of nitrifying bacteria of compost bedding obtained from sheep farm which have important role in reducing odor emission of ammonia. The 12 days, 1 and 1,5 months old of compost bedding samples were obtained from sheepfold, while 3 and 4 months old of compost samples were obtained from compost pile. Furthermore, nitrifying bacteria were isolated from compost samples by pour plate method using specific media for Nitrosomonas and Nitrobacter. The results indicated that compost bedding samples with different composting time duration displayed different abundance and diversity of nitrifying bacteria. The current study was successfully isolated 39 and 47 nitrifying bacterial isolates using specific media for Nitrobacter and Nitrosomonas, respectively. The highest diversity of nitrifying bacteria was gained from 12 days old compost bedding sample. Nitrifying bacterial isolates from compost bedding samples have significant role in odor emission declining as well as manure composting at sheep farm. The obtained bacterial isolates are also potentially to develop as bio-activator for compost bedding.
EFEK POLIMORFISME GENA GSTP-1 TERHADAP AKTIVITAS GLUTATION S-TRANSFERASE (GST) PADA INDIVIDU TERPAPAR LOGAM BERAT TIMBAL (Effect of GSTP-1 Gene Polymorphismson Glutation S- Transferase (GST) Activity in Heavy Metals Lead-Exposed Individual) Hernayanti Hernayanti; Agung Saprasetya Dwi Laksana; Saefuddin Aziz
Jurnal Manusia dan Lingkungan Vol 22, No 3 (2015): November
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18755

Abstract

ABSTRAKGena GSTP-1 merupakan penghasil enzim glutation S- transferase (GST), yang berfungsi dalam proses detoksifikasi senyawa toksik di hati. Faktor keberadaan polimorfisme gena GSTP-1 akan menyebabkan penurunan ekspresi GST, sehingga proses detoksifikasi terhadap senyawa toksik akan terhambat. Kerentanan terhadap paparan senyawa toksik pada manusia akan meningkat apabila dijumpai polimorfisme gena. Salah satu senyawa toksik yang dapat menghambat aktivitas GST adalah timbal (Pb), terutama dalam bentuk tetra ethyl lead (TEL). Tujuan penelitian adalah untuk mengetahui pengaruh polimorfisme gena GSTP-1 terhadap aktivitas GST pada individu terpapar Pb, yang diwakili pekerja bengkel mobil. Faktor keberadaan polimorfisme gena individu ditentukan dengan metode PCR-RFLP dan enzim restriksi BsmA1. Parameter yang diukur adalah kadar Pb dan aktivitas GST. Analisis molekuler gena GSTP-1 dilakukan secara deskriptif. Data kadar Pb dan aktivitas GST dianalisis dengan uji t independent. Hasil analisis gena GSTP-1 dari 40 orang subyek kasus setelah dilakukan digesti dengan enzim BsmA1, ditemukan sebanyak 10 orang individu dengan polimorfisme Ile105Val gena GSTP 1 atau sekitar 25% dengan genotip Ile-Val, sedangkan 30 orang atau 75% ditemukan tanpa polimorfisme dengan genotip Ile-Ile. Pita DNA individu dengan polimorfisme terpotong menjadi 3 fragmen sepanjang 176, 91 dan 85 pp (mutan heterozygot), sedangkan tanpa polimorfisme terletak pada 176 bp. Subyek kasus dengan polimorfisme gena GSTP-1 memiliki kadar Pb lebih tinggi dan aktivitas GST lebih rendah dibandingkan individu non polimorfisme. Telah terbukti bahwa polimorfisme gena GSTP-1 menyebabkan penurunan ekspresi enzim GST. Pada individu terpapar Pb dengan polimorfisme gena GSTP-1 memiliki aktivitas GST lebih rendah dibandingkan individu tanpa polimorfisme.ABSTRACTGSTP-1 gene regulates the expression of gluthation S-transferase enzyme, which role in detoxification of toxicant on liver. If the polymorphisms gene is found in individual, the production of GST is decreased and the enzyme failed to eliminate toxicants. Lead is one of toxic agents that could inhibite GST activity especially tetra ethyl lead (TEL). The susceptibility to lead exposure will increase if the polymorphisms gene is found in population. The objective of this studies were to know the effect of gene GSTP-1 polymorphisms to GST activity on lead-exposed individual ie. autorepair workers. The genotype individu were analyzed by Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphisms with BsmA1 restriction enzyme followed by descriptived analyzed. Parameter recorded were blood lead and GST activity and data were analyzed by independent t-test. These result showed that 25% of 40 individual cases subject were detected by enzyme BsmA1 as polymorphisms individual of GSTP-1 gene, with Ile105Val genotype. As many as 75% were detected as non polymorphisms with Ile-Ile genotype. Three fragment DNA of polymorphisms individual of GSTP-1 is located on 176, 91 and 85 bp (heterozygote mutant) but non polymorphisms individual is only located on 176 bp. The Pb level of individual with polymorphisms GSTP-1 gene is higher than non polymorphisms individual but their GST activity was lower than non polymorphisms individual. It could be concluded that polymorphisms GSTP-1 gene could decrease the expression gene of GST enzyme and intoxication of lead-exposured could increased the decreasing of this activity.
Co-Authors . HERNAYANTI Adi Amurwanto Afifah Mariana Agung Saprasetya Dwi Laksana Agung Saprasetya Dwi Laksana, Agung Saprasetya Dwi AGUS HERY SUSANTO Agus Sabdono Alfi Muntafiah Alice Yuniaty Anwar Rovik Budi Rustomo Daniel Joko Wahyono Dini Ryandini Dwiana Muflihah Yulianti Dyah Fitri Kusharyati Fitranto Arjadi Hendro Pramono Hendro Pramono Hendro Pramono Hernayanti Hernayanti Meyta Pratiwi Nurtjahjo Dwi Sasongko Ocky Karna Radjasa Oedjijono Ratna Stia Dewi Riyanti Rizal Khoirun Alfisah Shoffiana Noor Sri Martina Wiraswati Taruna Dwi Satwika Yuriza Eshananda