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All Journal Agrikultura Biotika: Jurnal Ilmiah Biologi Jurnal Agroekoteknologi Jurnal Agro Kultivasi AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Media Kontak Tani Ternak
Mieke Rochimi Setiawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Social Sciences

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Viability test of halotolerant nitrogen-fixing rhizobacteria on different carrier composition and application dosage of nitrogen biofertilizer to increase rice growth on saline ecosystems Fiqriah Hanum Khumairah; Fachruddin Azwari; Mieke Rochimi Setiawati; Betty Natalie Fitriatin; Tualar Simarmata
Kultivasi Vol 21, No 1 (2022): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v21i1.33068

Abstract

Abstract The use of saline soils as productive agricultural land poses major challenges. The utilization of nitrogen biofertilizer with halotolerant N-fixing rhizobacteria as the active material at the right dosage can increase soil productivity and support plant growth. The aim of this study was to obtain the composition of the carrier material that can maintain rhizobacteria viability, water content, and pH of nitrogen biofertilizer and to obtain the right dosage to increase the growth of rice plants in saline ecosystems. The research location was at Microbiology Laboratory of CV Bintang Asri Arthauly, Bandung and greenhouse of Jayamukti Village, Banyusari District, Karawang Regency from February to November 2020 used completely randomized design. The viability test consisted of nine treatments, while the application dosage test consisted of 13 treatments and repeated three times. The result showed that the H carrier composition (50% peat + 17.5% compost + 17.5% biochar + 5% dolomite + 5% guano + 5% nutrition) was able to maintain high viability of halotolerant N-fixing rhizobacteria compared to other treatments (10.22 x 107 CFU mL-1). Water content (34.50%) and pH level (7.9) in the composition H also meet the quality standard requirements of the biofertilizer, respectively. Nitrogen biofertilizer with H carrier composition at a dosage of 1500 g ha-1 applied to seed and nursery can increase the height and biomass of rice plants grown under saline condition. Further research is needed on the application of nitrogen biofertilizers in saline soil that can increase the effectiveness of N fertilization.Keywords: Carrier ∙ Rhizobacteria ∙ Rice ∙ Saline ecosystem ∙ Viability  AbstrakPenggunaan tanah salin sebagai lahan pertanian produktif memiliki tantangan yang besar. Pemanfaatan pupuk hayati nitrogen dengan rhizobakteri penambat N halotoleran sebagai bahan aktifnya pada dosis yang tepat dapat meningkatkan produktivitas tanah dan mendukung pertumbuhan tanaman padi. Penelitian bertujuan mendapatkan komposisi bahan pembawa yang dapat mempertahankan viabilitas rhizobakteri, kadar air, dan pH pupuk hayati nitrogen serta mendapatkan dosis yang tepat untuk meningkatkan pertumbuhan tanaman padi pada ekosistem salin. Lokasi penelitian di Laboratorium Mikrobiologi CV Bintang Asri Arthauly Bandung dan rumah kaca Desa Jayamukti, Kecamatan Banyusari, Kabupaten Karawang sejak bulan Februari sampai November 2020. Metode percobaan menggunakan metode eksperimental dengan Rancangan Acak Lengkap. Uji viabilitas terdiri dari sembilan perlakuan, sedangkan uji dosis aplikasi pupuk hayati terdiri dari 13 perlakuan dan masing-masing diulang sebanyak tiga kali. Hasil menunjukkan bahwa komposisi bahan pembawa H (Gambut  50% + kompos 17.5% + biochar 17.5% + dolomit 5% + guano 5% + nutrisi 5%) mampu mempertahankan viabilitas rhizobakteri penambat N halotoleran yang tinggi dibandingkan perlakuan lainnya yaitu sebesar 10,22 x 107 CFU/mL. Kadar air dan pH level pada komposisi H  juga memenuhi syarat baku mutu pupuk hayati yaitu sebesar 34.50% dan 7.9. Pupuk hayati dengan komposisi bahan pembawa H dengan dosis 1500 g ha-1 yang diaplikasikan pada benih dan persemaian mampu meningkatkan tinggi dan biomassa tanaman padi yang ditanam pada kondisi salin. Perlu penelitian lebih lanjut mengenai aplikasi pupuk hayati N di tanah salin yang dapat meningkatkan efektivitas pemupukan N. Kata Kunci: Bahan pembawa ∙ Ekosistem salin ∙ Padi ∙ Rhizobakteria ∙ Viabilitas
Perbedaan Konsentrasi Pupuk Hayati Cair Berbasis Azolla terhadap Pertumbuhan dan Hasil Tanaman Pakcoy (Brassica rapa L.) Mieke Rochimi Setiawati; Eka Safitri; Shinta Nurrizqi Indrayani; Evi Entang Fatimah; Nurullita Fitri Qurnia; Reginawati Hindersah; Pujawati Suryatmana
Jurnal Agroekoteknologi Vol 13, No 2 (2021)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v13i2.13161

Abstract

Aplikasi pupuk hayati cair berbasis Azolla merupakan suplemen pemupukan pada tanaman sayuran yang memanfaatkan potensi alam di sekitar lahan pertanian. Berbagai penelitian mengungkapkan konsentrasi aplikasi pupuk hayati cair beragam sehingga untuk efisiensi penggunaan pupuk hayati cair perlu dicari konsentrasi yang tepat.  Tujuan penelitian ini adalah menganalisis respon tanaman pakcoy melalui pemberian konsentrasi pupuk hayati yang berbeda terhadap peningkatan pertumbuhan dan hasil tanaman pakcoy. Penelitian ini menggunakan 7 perlakuan dengan 6 ulangan yang disusun berdasarkan rancangan acak kelompok (RAK). Perlakuan percobaan terdiri atas  : tanpa pemberian pupuk hayati (K0), 5 ml/L pupuk hayati (K1), 10 ml/L pupuk hayati (K2), 15 ml/L pupuk hayati (K3), 20 ml/L pupuk hayati (K4), 25 ml/L pupuk hayati (K5), 30 ml/L pupuk hayati (K6). Hasil penelitian menunjukkan bahwa konsentrasi pupuk hayati mulai dari 5 sampai 30 mL/L dapat meningkatkan tinggi tanaman, jumlah daun, lebar daun, panjang daun, dan luas daun serta bobot basah tanaman pakcoy dibandingkan tanpa pemberian pupuk hayati. Konsentrasi pupuk hayati 10 mL/L menghasilkan jumlah daun pakcoy yang lebih tinggi dibandingkan kontrol akan tetapi tidak berbeda dibandingkan dengan bobot basah tanaman pakcoy pada setiap konsentrasi pupuk hayati yang diberikan. Pemberian pupuk hayati dengan konsentrasi 10 ml/L meningkatkan bobot basah tanaman pakcoy dua kali lipat atau 100,74 % dibandingkan kontrol. Pupuk hayati cair berbasis azolla dapat digunakan sebagai nutrisi tambahan disamping pupuk NPK untuk meningkatkan pertumbuhan dan hasil tanaman pakcoy.
PENGARUH PUPUK ORGANIK (Azolla pinnata) TERHADAP C-ORGANIK TANAH, SERAPAN N DAN BOBOT KERING TANAMAN PADI (Oryza sativa L.) PADA TANAH DENGAN TINGKAT SALINITAS TINGGI Mayang Sunduz Arafah; Mieke Rochimi Setiawati; Anne Nurbaity
Jurnal Agroekoteknologi Vol 9, No 1 (2017)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v9i1.5040

Abstract

Utilizing organic fertilizer Azolla pinnata is needed especially for rice commodity which planted in salin soil in coast area for increasing the efficiency of inorganic fertilizer by adding organic matters. This research was aimed to get the best combination of utilizing organic fertilizer Azolla pinnata to increase total organic carbon, nitrogen uptake, and dry weight of rice in saline soil. This research used Randomized Complete Block Design experiment which consisted of eight combinations of treatment. Those are treatment of salinity 0, 2, 4 and 6 mmhos cm-1 + without utilizing organic fertilizer Azolla pinnata and treatment of salinity 0, 2, 4, and 6 mmhos cm-1 + utilizing organic fertilizer Azolla pinnata. The result showed that utilizing organic fertilizer Azolla pinnata can reduce the negative effect from salinity which proven by increased N uptake of rice in treatment of salinity 6 mmhos cm-1.Keywords: Azolla pinnata, Rice (Oryza sativa L.), Soil with high salinity level
Aplikasi Bakteri Endofitik Penambat N2 untuk meningkatkan Populasi Bakteri Endofitik dan Hasil Tanaman Padi Sawah Mieke Rochimi Setiawati; Dedeh H. Arief; Pujawati Suryatmana; Ridha Hudaya
Agrikultura Vol 19, No 3 (2008): Desember, 2008
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.21 KB) | DOI: 10.24198/agrikultura.v19i3.1009

Abstract

Bakteri endofitik penambat nitrogen (N2) yang bersimbiosis dengan tanaman padi dapat digunakan sebagai pupuk hayati karena dapat memberikan nitrogen yang ditambatnya langsung pada tanaman. Penelitian pot ini bertujuan untuk mendapatkan konsentrasi dan teknik aplikasi pupuk hayati cair bakteri endofitik penambat N2 yang tepat dalam meningkatkan populasi bakteri endofitik penambat N2 dan  hasil tanaman padi sawah.  Percobaan dirancang dalam Rancangan Acak Kelompok yang menguji faktor konsentrasi pupuk hayati (25, 50, 75 dan 100 ml l-1 pupuk hayati) dan metode aplikasi pupuk (Perendaman, Penyemprotan dan kombinasinya) . Hasil percobaan memperlihatkan adanya peningkatan populasi bakteri endofitik penambat N2 di daun, batang, dan akar setelah aplikasi pupuk hayati baik melalui teknik perendaman, penyemprotan maupun kombinasinya. Aplikasi  100 ml l-1 pupuk hayati melalui semua teknik menghasilkan gabah kering panen 85,89 g tanaman-1, lebih besar  7,79 %  daripada penggunaan 50 ml l-1 pupuk hayati.
Seleksi dan Aplikasi Bakteri Endofitik Penambat N2 Asal Tanaman Padi dari Tanah Salin Pantura Indramayu dalam Meningkatkan Fiksasi N 2 , Serapan N dan Bobot Kering Tanaman Padi Gogo (Oryza sativa L.) Mieke Rochimi Setiawati
BIOTIKA Jurnal Ilmiah Biologi Vol 11, No 1 (2013): BIOTIKA JUNI 2013
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/bjib.v11i1.10049

Abstract

Aplikasi pupuk hayati ameliorant, dan pupuk NPK terhadap N total, P tersedia serta pertumbuhan dan hasil jagung pada inceptisols Mieke Rochimi Setiawati; Lia Nur Linda; Nadia Nuraniya Kamaluddin; Pujawati Suryatmana; Tualar Simarmata
Jurnal Agro Vol 8, No 2 (2021)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/15121

Abstract

Inceptisols umumnya memiliki tingkat kesuburan tanah rendah sampai sedang. Upaya peningkatan ketersediaan hara tanah Inceptisol dengan pupuk anorganik NPK perlu diimbangi dengan aplikasi pupuk hayati dan amelioran organik. Percobaan ini bertujuan untuk mengurangi dosis pupuk NPK dengan menggunakan pupuk hayati dan amelioran organik serta meningkatkan hasil tanaman jagung. Percobaan dilakukan di Pasir Banteng, Kabupaten Sumedang. Penelitian menggunakan RAK faktorial. Pupuk hayati dengan amelioran organik sebagai faktor pertama sebanyak empat taraf: tanpa pupuk hayati dan amelioran (kontrol), diberi pupuk hayati, amelioran organik, dan gabungan keduanya. Faktor kedua yaitu dosis pupuk NPK empat taraf: 100%, 80%, 60%, 40% NPK dosis anjuran.  Dilakukan tiga kali ulangan. Hasil penelitian menunjukkan terdapat interaksi antara pupuk hayati, amelioran organik, dengan pupuk NPK terhadap tinggi tanaman, N-total tanah, bobot tongkol berkelobot dan tanpa kelobot. Pemberian pupuk hayati dan amelioran disertai pupuk NPK 100% dan yang tanpa diberi amelioran menghasilkan bobot tongkol tanpa kelobot sebesar 1.089 g dan 1.064 g per tanaman. Pemberian pupuk hayati dengan amelioran dan pemberian pupuk NPK 100% menghasilkan bobot pipilan per tanaman 526,08 g dan 539,08 g. Aplikasi pupuk hayati dan amelioran organik belum mampu mengurangi dosis penggunaan pupuk NPK pada Inceptisols asal Pasir Banteng. Inceptisols generally have low to moderate soil fertility. The effort to increase the nutrients availability in Inceptisol through the application of NPK fertilizers need to be balanced with biofertilizers and organic ameliorants. This experiment aimed to reduce the dose of NPK fertilizer by using biofertilizers and organic ameliorants and to increase maizeyields. The experiment was conducted in Pasir Banteng, Sumedang Regency. The factorial RBD was used. The biofertilizer with organic ameliorant as the first factor: no biofertilizer and no ameliorant (control), biofertilizer, organic ameliorant, and combination of both. The second factor was dose of NPK fertilizer: 100%, 80%, 60%, 40% of recommended NPK dose. It was repeated three times. The results showed that there was interaction between biofertilizer, organic ameliorants, with NPK fertilizers on plant height, N-total soil, weight of cobs with and without husk. The application of biofertilizer and ameliorant with 100% NPK fertilizer and without ameliorant were 1,089 g and 1,064 g of cobs without husks, respectively. The application of biofertilizer with ameliorant and 100% NPK fertilizer had a grain weight of 526.08 g and 539.08 g, respectively. The application of biofertilizers and organic ameliorants has not been able to reduce the dose of NPK fertilizer on Inceptisols from Pasir Banteng.
KARAKTERISTIK PERTUMBUHAN DAN WAKTU GENERASI ISOLAT Azotobacter sp. DAN BAKTERI ENDOFITIK ASAL EKOSISTEM LAHAN SAWAH Mieke Rochimi Setiawati; Pujawati Suryatmana; Diyan Herdiyantoro; Zahra Ilmiyati
Jurnal Agroekoteknologi Vol 6, No 1 (2014)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.49 KB) | DOI: 10.33512/j.agrtek.v6i1.222

Abstract

Azotobacter sp. and N2-fixation endophytic bacteria are microbes that play a role in fixation the N2 from the free air and helps supply of N to plants and can make efficient use of N derived from inorganic fertilizer. N2-fixation microbes utilization in rice fields can reduce the cost of production of rice plants. By studying the characteristics of the two types of beneficial bacteria that is expected to be engineered to enhance the role of these bacteria contribute in supplying N to rice crops. The method used in this study is exploratory and observe the characteristics of growth and generation time of bacteria for 72 hours. The results of this study indicate that the bacterial isolates of Azotobacter sp-1 has the highest population in 48 hours with a total population of 1.58 x107 cfu ml-1 , whereas bacteria Azotobacter sp-2 has the highest population in 60 hours with a total population of 3.12 x106 cfu ml-1 . Both N2-fixation endophytic bacteria populations reached the highest peak in the 60th hour with a total population of N2-fixation endophytic bacteria-1 at 1.12 x 108 cfu ml-1 of N2-fixation endophytic bacteria-2 of 9.4 x 107 cfu ml-1 of both isolates endophytic bacteria showed a similar growth pattern. Azotobacter sp. isolates from rhizosfir of the rice plant have a difference generation time, while the two isolates of N2-fixation endophytic bacteria from tissue of rice plants having the same generation time. Isolates of Azotobacter sp.-1 had the fastest of generation time, capable of generating new cells every 158.66 minutes or every 2.64 hours.
Uji Viabilitas Isolat Bakteri Penambat Nitrogen Halotoleran pada Komposisi Bahan Pembawa yang Berbeda Nida Uli Al-Azmiya; Fiqriah Hanum Khumairah; Mieke Rochimi Setiawati; Tualar Simarmata
Jurnal Agroekoteknologi Vol 13, No 1 (2021)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v13i1.12165

Abstract

The process of microbial inoculation into plant biomass which will be decomposed needs a suitable carrier material to facilitate the application of the microorganisms to be used. Halophilic bacteria are a type of microorganism that can survive high salt levels by maintaining an osmotic balance. The bacterial consortium is a collection of bacteria that work together to form a community, to produce a significant product. The experiment aims to determine the effect of carrier composition on inoculant viability. The experiment was carried out at the laboratory CV. Bintang Asri Arthauly Bandung, West Java, Indonesia. The study used a non factorial randomized design with three replications. The treatment design to be tested in this experiment, among others: c1: Peat 50% + Compost 50% + Nutrition 0%, c2: Peat 50% + Compost 45% + Nutrition 5%, c3: Peat 50% + Compost 40% + Nutrition 10%, c4: Peat 50% + Compost 25 % + Biochar 25% + Nutrition 0%, c5: Peat 50% + Compost 22.5% + Biochar 22.5% + Nutrition 5%, c6: Peat 50% + Compost 20% + Biochar 20% + Nutrition 10%, c7: Peat 50% + Compost 20% + Biochar 20% + Dolomite 5% + Guano 5% + Nutrition 0%, c8: Peat 50% + Compost 17.5% + Biochar 17.5% + Dolomite 5% + Guano 5 % + Nutrition 5%, c9: Peat 50% + Compost 15% + Biochar 15% + Dolomite 5% + Guano 5% + Nutrition 10%. The results of the second stage experiment showed that the c8 composition had the best viability in all carrier compositions.
RETARDED GROWTH OF LOWLAND RICE IN SALINE SOIL INOCULATED WITH NITROGEN-FIXER AZOTOBACTER Reginawanti Hindersah; Alia Halimatusy; Benny Joy; Mieke Rochimi Setiawati; Diyan Herdiyantoro
Jurnal Agroekoteknologi Vol 12, No 1 (2020)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v12i1.8782

Abstract

Low-land rice (Oryza sativa L.) cultivation in saline soils face some constraints include nitrogen availability. Saline-resistant nitrogen fixing bacteria Azotobacter are expected to increase supply nitrogen in saline soils. The objectives of the study were to determine the effect of liquid inoculant concentration of two Azotobacter isolates on early vegetative growth of lowland rice grown in potted saline soil.  The greenhouse trial design was a randomized block design with seven treatments and four replications. The treatments were combination of isolates and Azotobacter liquid inoculant concentrations in single and mixed inoculation. The results showed that all plants experienced chlorosis and stunt due to high Electrical Conductivity. Inoculation of different isolates and concentrations did not influence the growth of lowland rice in soil with high EC at the end of experiment. Therefore, neither isolates nor concentration of Azotobacter could improve retarded-growth of lowland rice in saline soil.
PENGARUH PUPUK HAYATI PADAT TERHADAP SERAPAN N DAN P TANAMAN, KOMPONEN HASIL DAN HASIL PADI SAWAH (Oryza sativa L.) Mieke Rochimi Setiawati; Emma Trinurani Sofyan; Zaenal Mutaqin
Jurnal Agroekoteknologi Vol 8, No 2 (2016)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/j.agrtek.v8i2.1485

Abstract

Optimization of production rice plants can be achieved if the needs of macro and micro nutrients essential plant fulfilled. To achieve optimal rice yield can be done by proper fertilization based on the type, characteristics and nutrient content of fertilizers applied. Alternative to increasing rice yield can be done by applying biofertilizer in reducing the use of inorganic fertilizers. This experiment aims to determine the effect of Solid Biofertilizer (SBF) on nutrient uptake, growth, and yield and yield components of rice paddy fields. This research was carried out on the planting medium Inceptisols at Jatinangor used a Randomized Completely Block Design (RCBD) with 10 treatment combinations which replicated 3 times. The results showed that a dose of 1 NPK, combined with SBF ranging from ¼ dose, dose ½, ¾ and 1-dose showed gradual improvement to the uptake of N and P as well as yield and yield components of paddy.Keywords: Solid Biofertilizer, NPK Fertilizer, Paddy Yield
Co-Authors Alia Halimatusy Alin Kusumah Dewi Anne Nurbaity Azwari, Fachruddin Benny Joy Betty Natalie Fitriatin Dahlia Florencia Manurung Dedeh H. Arief DIYAN HERDIYANTORO Eka Safitri Emma Trinurani Sofyan Evi Entang Fatimah Gordon Pius Marihot Khumairah, Fiqriah Hanum Lia Nur Linda Mayang Sunduz Arafah Nadia Nuraniya Kamaluddin Nanda Aditya Setyawan Nida Uli Al-Azmiya Nurullita Fitri Qurnia Pujawati Suryatmana Reginawanti Hindersah Reginawati Hindersah Ridha Hudaya Shinta Nurrizqi Indrayani Tualar Simarmata Ummu ‘Azizah Halimah Zaenal Mutaqin Zahra Ilmiyati