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All Journal Jurnal Kesehatan Prima Jurnal Analis Medika Biosains (JAMBS) The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy

Maruni Wiwin Diarti

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Author-ID : 2390830

Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Nursing Public Health

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Formulasi Bubuk Bunga Cengkeh (Syzygium Aromaticum) Dalam Media Teri Ubi Rumput Laut Medium (Turlm) Formula 3 Sebagai Zat Penghambat Jamur Kontaminan Pada Biakan Mycobacterium Tuberculosis rohmi rohmi; Fahrudin Ahad; Maruni Wiwin Diarti
Jurnal Analis Medika Biosains (JAMBS) Vol 7, No 1 (2020): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v7i1.176

Tujuan penelitian ini adalah mengetahui Formulasi Bubuk bunga Cengkeh (Syzygium aromaticum ) dalam Media Teri Ubi Rumput Laut Medium (TURLM) Formula 3 Sebagai Zat Penghambat Jamur Kontaminan pada Biakan Mycobacterium tuberculosis yang dapat digunakan dalam menghambat pertumbuhan jamur kontaminan tanpa mempengaruhi pertumbuhan koloni dan angka kesuburan M. tuberculosis. Desain penelitian eksperiment di laboratorium dengan menggunakan desain The Posttest Only Control Group Design. Data dari hasil pengamatan pertumbuhan koloni pada masing – masing media formula kombinasi Bubuk bunga CengkehTURLM Formula 3 (tiga) dan media pembandingan Loweinstein – Jensen dilakukan analisis secara deskriptif. Hasil penelitian menunjukkan bahwa gambaran tekstur media antara ketiga formula TURLM 3 dengan penambahan bubuk cengkeh tidak menunjukkan perbedaan yang signifikan baik dalam tekstur kekerasan, homogenitas, bau dan permukaan. Media TURLM formula 3 tanpa bubuk cengkeh dan Loweinstein – Jenssen menunjukkan pertumbuhan koloni jamur kontaminan Aspergillus flavus, Aspergillus niger dan bakteri kontaminan Staphylococcus albus, kecepatan pertumbuhan pada hari ke -5 dan angka kesuburan pertumbuhan 4 (empat), sedangkan pada media formulasi bubuk bunga Cengkeh (Syzygium aromaticum ) dalam media Teri Ubi Rumput Laut Medium (TURLM) formula 3 baik pada formulasi 1,2 dan 3 sampai dengan minggu ke -8 pengamatan menunjukkan hasil pertumbuhan koloni jamur kontaminan dan M. tuberculosis negative. Kesimpulan dari penelitian ini adalah media TURLM formula 3 yang ditambahkan bubuk cengkeh pada semua formula mampu membuktikan sangat efektif dalam menghambatan pertumbuhan jamur kontaminan, namun juga menghambat pertumbuhan M. tuberculosis, sehingga dapat dikembangkan untuk anti jamur pada semua produk media yang rentan terhadap kontaminasi jamur, terutama pada media pertumbuhan M. tuberculosis.

ANALISIS MOLEKULER MDR TB DENGAN TEKNIK SEKUENSING DARI SAMPEL DAHAK SUSPEK TB DI NUSA TENGGARA BARAT Edy Kurniawan; Maruni Wiwin Diarti; Santy Pristianingrum
Jurnal Kesehatan Prima Vol 9, No 1 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Background: Tuberculosis is one of the tropical diseases that are still of concern to the world , especially with the increasing number of developing multidrug- resistant TB variants. In this study we indetified nucleotide sequence MDR-Tb resistant rifampicin, etambhutol dan isoniazid from patient sputum who have suspect tuberculosis infection in West Nusa Tenggara. Methods: This analyze with PCR, sequencing and analysis using Blast Basic Local Alignment Search Tool) from NCBI (National Centre of Biotechnology Information). Result : Twelve samples has been obtained in this study, 9 is positive PCR with TB primer and all of them is still sensitive with rifampicin, etambuthol and isoniazid. Ten sequence sensitive from rpoB, emB and katG has sequencing and Blast analysis, sequence rpoB from TB 5 and TB 6 have similarity with sequence strain M.tb CCDC5079 and TB8 and TB 9 with sequence strain M.tb 96121. emB sequence from sample TB8 and TB9 have similarity with M.tb subsp.tuberculosis partial embB putative arabinosyltransferase mutan 240 exon 1; and TB9 with M.tb strain 2a putative arabinosyltransferase. Sequence from katG from TB8,TB9,TB10 have similarity with M.tb strain 96075. Conclution: MDR-Tb is not found in the studies, with Blast analysis we concluded the Mycobacterium tuberculosis sensitive tuberculosis drugs sequence from sample study have similarity with M.tb Beijing dan manila Family.

NATURAL MEDIA FOR THE GROWTH OF CANDIDA ALBICANS CAUSES OF CANDIDIASIS BY ARTOCARPUS COMMUNIS Yunan Jiwintarum; Urip Urip; Anas Fadli Wijaya; Maruni Wiwin Diarti
Jurnal Kesehatan Prima Vol 11, No 2 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

a candida albicans requires a source of nutrients especially carbohydrates that are sufficient for its growth. Semi-synthesis media is the most common medium for growing Candida albicans. Semi-synthesized media composes of natural and synthesis materials. An abundance of biological sources in nature with higher carbohydrate content than natural ingredients on semi-synthesized media encourage to find the materials of alternative media from different carbohydrate sources, particularly grains, one of them is breadnut seed. This study aimed to find out a potential of Artocarpus communis as an alternative media for the growth of Candida albicans causes of candidiasis. This study was true experiment, used 6 replications and 4 treatments with PDA as control media, the media of Artocarpus communis with concentration of 10%, 20%, and 30%. The data of Candida albicans growth was tested statistically used One Way Anova with 95% confidence level or α = 0.05. The result indicated that a number of Candida albicans growth on PDA media was 13.7 colonies, on alternative media of Artocarpus communis with the concentration of 10% was an average of 13.5 colonies, the concentration of 20% was an average of 16.3 colonies and the concentration of 30% was an average of 24 colonies. The conclusion: the Artocarpus communis can be employed as an alternative natural media of Candida albicans growth.

TOTAL KOLESTEROL CONDITIONS ON CONSUMPTION OF BLACK POWDER COFFEE (Traditional) WITH PATIENT PREPARATION Maruni Wiwin Diarti; Yunan Jiwintarum; Ida Ayu Reka
Jurnal Kesehatan Prima Vol 12, No 1 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: A traditional black coffee powder is one of the beverages that excessively consumed by communities. The increasing of total cholesterol level in blood is influenced by the habit of consuming coffee. Coffee has chemicals that can cause some health problems, namely cafestol and kahweol. The objective study was to determine the effect of patient preparation against the result of total cholesterol levels examination on the consumers of traditional black coffee powder. This research was analytic observational. The sample was capillary blood of consumers’ a traditional black coffee as many as 30 people. Statistical analysis utilized Mann-Whitney U Test with 95% confidence level (α = 0,05). The findings indicated the average deviation of total cholesterol level after fasting 12,86 mg / dL and mean difference after giving coffee 7 cups 18,46 mg / dL. Mann Whitney Test found significant value 0.001 <α = 0.05 which means there is an influence of patient preparation against the result of total cholesterol levels examination on the consumers of traditional black coffee powder.Keywords: Total Cholesterol Level; Consumers of Traditional Black Coffee Powder.

PROFIL LEUKOSIT TIKUS PUTIH YANG DIBERI AIR SEDUHAN KELOPAK BUNGA ROSELA MERAH (Hibiscus sabdariffa) Pancawati Ariami; Hesti Suliastiningsih; Maruni Wiwin Diarti
Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Roselle is a plant that has many benefits with vitamin C, iron ,folic acid , and amino acids highly needed by the body. This study aimed to determine the effect of the provision of water steeping red roselle calyx (Hibiscus sabdariffa) on peripheral blood leukocyte counts in rats (Rattus norvegicus) Wistar strain. Pre Experimental Research done. The results showed the average number of leukocytes in peripheral blood of experimental animals before treatment was 4060 / µL, and after the treatment is 8120 / µL . This shows that red roselle calyx (Hibiscus sabdariffa) can increase the number of leukocytes of 4060 / µL . Based on the results of statistical tests Paired T - Test showed no significant difference steeping water provision red roselle calyx (Hibiscus sabdariffa) on peripheral blood leukocyte counts in rats (Rattus norvegicus) Wistar strain, the probability of 0.004 < α 0.05. Provision of water steeping red roselle calyx (Hibiscus sabdariffa) effect on the number of leukocytes in peripheral blood white rats (Rattus norvegicus) Wistar strain . The number of leukocytes increased 2 -fold with water provision steeping red roselle calyx after 9 days of treatment with a concentration of 2 % .

A CHARACTERISTIC OF MORPHOLOGY, COLONY AND BIOCHEMISTRY OF BACTERIA THAT ISOLATED FROM SEDIMENTS OF MOSQUITO BREEDING LAGOON Maruni wiwin Diarti; Rohmi rohmi; Yuri Syaffitri Kinanti Achmad; Yunan Jiwintarum
Jurnal Kesehatan Prima Vol 11, No 2 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

The lagoon is a form of semi-enclosed bay which is one type of an estuary. In lagoon water there is a meeting and mixing of freshwater and seawater thus it causes free relationship between the sea and the source of freshwater. The effect of tides that mixed with freshwater will cause a change of the aquatic character instantly. It will certainly affect the diversity of organism species, including bacteria. Some types of bacteria, one of them is Bacillus, it is basically soil bacteria, but commonly found in fresh and brackish waters. The purpose of this study was to determine the characteristics of morphology, colonies and biochemistry of bacteria that isolated from sediments of mosquito breeding lagoon in the coast of Medana village, Tanjung district, North Lombok regency. This research was descriptive observational, used non random purposive sampling with 15 samples. Data collected in the form of types of bacteria found in 15 points from sediments of mosquito breeding lagoon. The data analysis used descriptive analysis. The results of 15 points of lagoon sediments that isolated, from 15 bacteria colonies that was identified as morphological, colonies and biochemical characteristics found three types of bacteria: Aeramonas schubertii, Bacillus subtilis, and Enterbacter agglomerans. The conclusion is the sediments of mosquito breeding lagoon in the coast of Medana Village, Tanjung district, North Lombok regency is obtained the bacteria, for instace: Aeramonas schubertii, Bacillus subtilis, and Enterobacter agglomerans.

THE EFFECT OF MELON SEEDS FLOUR (Cucumis Melo L.) TOWARD TOTAL CHOLESTEROL LEVEL IN WHITE MALE WISTAR STRAIN RATS (Rattus Norvegicus) Maruni Wiwin Diarti; Erlin Yustin Tatontos; Yunan Jiwintarum; Anggun Sari Mianti
Jurnal Kesehatan Prima Vol 12, No 2 (2018): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Cholesterol is an important element in the body, but in high quantities, it can cause atherosclerosis that will eventually possess an impact on coronary heart disease. One of the foods that can reduce cholesterol is a food contains flavonoids which is found in melon seeds (Cucumis melo l.). It contains high fiber that can reduce either of excessive insulin or blood fat levels. The objective study was to determine the effect of Melon seeds flour Toward Total Cholesterol Level in White Male Wistar Strain Rats. This research was carried out with pre-expriment research design, used One group pretest-postest with 5 male wistar strain rats. Data collected in the form of data examination results of total cholesterol level before and after introducing Melon seeds flour. The mean result of total cholesterol test in white male rats before giving Melon seeds flour was 261 mg / dL, whereas the result of total cholesterol test after giving Melon seeds flour was 190.2 mg/dL. Cholesterol levels decreased by 70.8 mg/dl. In short, presenting Melon seeds flour (Cucumis melo l.) for 14 days can decrease total cholesterol level in white male wistar rats. Keywords: Melon Seeds, wistar strain, total cholesterol.

RESISTENSI PRIMER MYCOBACTERIUM TUBERCULOSIS DENGAN TARGET Gen rpoB Yunan Jiwintarum; Maruni Wiwin Diarti; Awan Dramawan
Jurnal Kesehatan Prima Vol 8, No 1 (2014): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: Detection of primary resistance in sputum samples of patients with pulmonary tuberculosis AFB (+) with molecular analysis on the target rpoB gene causes anti-tuberculosis drug resistance (OAT) rifampicin can be beneficial to the holder of a policy program to look for tuberculosis control efforts, one of attempt to overcome these problems is to deliver information about the resistance report in this interval. This research observasional descriptive study, the research variables Mycobacterium tuberculosis rpoB gene mutations and patients with pulmonary TB sputum smear (+), with the number of 50 sputum samples with acidental sampling technique. This study results showed all the samples (50 samples) microscopic examination of smear and PCR diagnostics with primary genes and gene Tb1-Tb2 results are positive (+) tuberculosis (100%). By nested PCR amplification techniques to prove that the primary gene rpoB1 negative results of 23 samples (46%), with the primary gene rpoB2 negative results in 13 samples (26%). Conclusion: A total of 13 samples (26%) occurred mutation in the gene rpoB / is resistant to the antibiotic rifampin.

KADAR ENZIM KHOLINESTERASE DARAH PETANI TERPAPAR PESTISIDA YANG DIBERIKAN RIMPANG TEMULAWAK (Curcuma xanthorrhiza Roxb) Haerul Anam; Nurhidayati Nurhidayati; Maruni Wiwin Diarti; Zaenal Fikri
Jurnal Kesehatan Prima Vol 9, No 2 (2015): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract: This study aims to determine the potential use of ginger rhizome (Curcuma xanthorrhiza Roxb) in increasing levels of enzyme kholinesterase on farmers who are exposed to toxic pesticides.The research method used is pre - posttest Control Design. In this research, there are 3 groups of samples each of 30 persons, ie negative control group who did not experience organophosphate and carbamate pesticide poisoning, the positive control group, farmers who suffered pesticide poisoning organophosphates and carbamates but not given the rhizomes of ginger (Curcuma Roxb xanthorrhiza), and treatment groups, farmers who experienced pesticide poisoning were organophosphates and carbamates and ginger rhizome (Curcuma xanthorrhiza Roxb.) Given dose of ginger is 3 grams / day for 9 days. Measurement of blood cholinesterase enzyme levels using tintometer kit. The results are classified on toxin a severe, moderate, mild and not toxin. Result were tested with Wilcoxon Signed Ranks test and Mann-Whitney Test, with 95% confidence level (p 0.05). The research proves that there are significant differences in levels of blood cholinesterase enzyme treatment groups before and after treatment (p 0.05) and no significant differences in levels of blood cholinesterase enzyme treatment group and positive control groups after the study (p <0.05). The conclusion of this study, administration of ginger rhizome (Curcuma Roxb xanthorrhiza) with a dose of 3 grams / day for 9 days can significantly increase levels of plasma cholinesterase enzyme in the farmers who suffered organophosphate pesticide poisoning and Carbamat. Therefore, the researchers recommend that farmers consume a dose of ginger to prevent and reduce organophoshate pesticide poisoning and Carbamat.

TOTAL CHOLESTEROL LEVELS IN TRADITIONAL COFFEE DRINKERS DUSUN SEMBUNG DAYE NARMADA LOMBOK DISTRICT WEST Maruni Wiwin Diarti; Iswari Pauzi; Siti Rif’ah Sabariah
Jurnal Kesehatan Prima Vol 10, No 1 (2016): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Abstract : Traditional coffee is one of the widely-consumed beverages Lombok Island community, one in Hamlet Sembung Daye. Coffee contains cafestol and kahweol substances that can raise cholesterol levels in the blood. This research is a descriptive study that aims to describe how the total cholesterol levels in the traditional coffee drinkers in the hamlet Sembung Daye Narmada District of West Lombok regency that the examination carried out using test methods strip. Data collected in the form of total cholesterol at 83 traditional coffee drinkers in the hamlet Sembung Daye Subdistrict Naramada West Lombok regency. The results showed the average total cholesterol level in males was 225 mg / dl and in women is 217 mg / dl. The conclusion of this study is total cholesterol levels in the traditional coffee drinkers in the hamlet Sembung Daye Narmada District of West Lombok district is more than the normal value.

Co-Authors Aden Turmuji Anas Fadli Wijaya Anggun Sari Mianti Awan Dramawan Edy Kurniawan Erlin Yustin Tatontos Fahrudin Ahad Haerul Anam Haris Widita Hesti Suliastiningsih I Gede Billy Sumantri I Gusti Ayu Nyoman Danuyanti Ida Ayu Reka Iswari Pauzi Nurhidayati Nurhidayati PANCAWATI ARIAMI Retno Handayani Rohmi Rohmi Rohmi Rohmi Santy Pristianingrum Siti Rif’ah Sabariah Soewignjo Soemohardjo Troef Sumarno Urip Urip Weny Astuti Yunan Jiwintarum Yuri Syaffitri Kinanti Achmad Zaenal Fikri Zainul Mutaqin

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