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ANALYSIS OF THE EXPRESSION OF FAS/CD95 AND HSP70 IN LOW AND HIGH GRADE UROTHELIAL CELL CARCINOMA OF THE BLADDER Heryanto Heryanto; Etty Hary Kusumastuti; Anny Setijo Rahaju
Folia Medica Indonesiana Vol. 53 No. 4 (2017): December 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.366 KB) | DOI: 10.20473/fmi.v53i4.7154

Abstract

Urothelial Cell Carcinoma (UCC), also called transitional cell carcinoma of the bladder, is the most common malignancy in urinary tract. The prognosis of this disease is highly dependent on the histological grading at diagnosis. Fas/CD95 has a role in apoptotic process, whereas HSP70 has an antiapoptotic role. This study aimed to analyze the expression of Fas/CD95 and HSP70 in low grade and high grade urothelial cell carcinoma in the bladder. This was an analytic observational study with cross sectional approach. Population and sample were paraffin blocks of urothelial cell carcinoma in the Laboratory of Anatomic Pathology, Dr. Soetomo Hospital, Surabaya in the period of January 2011-December 2016. Each grading of urothelial cell carcinoma was randomly sampled. Immunohistochemystry with Fas/CD95 and HSP70 were performed. Expression of Fas/CD95 and HSP70 were assessed semiquantitatively. Expression of Fas/CD95 and HSP70  were analyzed using Mann-Whitney test and Spearman test. The results showed there was significant different in expression of Fas/CD95 and HSP70 in low and high grades in urothelial cell carcinoma. There was no significant correlation between the expression of Fas/CD95 and HSP70 in urothelial cell carcinoma.  As a conclusion, the role of CD95 and HSP70 expression can be useful as marker for the diagnosis, especially in the determination of the grade of differentiation.
ORIGINAL ARTICLE: A COMPREHENSIVE STUDY OF PROSTATIC LESIONS AND ITS PROSTATE-SPECIFIC ANTIGEN LEVELS IN ANATOMICAL PATHOLOGY INSTALLATION OF RSUD DR. SOETOMO, SURABAYA FROM YEAR 2014 TO 2016 Izzan Khalidah Binti Muhamad; Anny Setijo Rahaju; Lukman Hakim
Jurnal Kedokteran Syiah Kuala Vol 19, No 1 (2019): Volume 19 Nomor 1 April 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v19i1.18049

Abstract

Abstract. Background: Diseases primarily affects prostate gland are inflammation, hyperplasia, and malignant tumour. Gleason score (GS) is an essential facet and together with PSA are substantial in diagnosing, managing, and determining the prognosis of CaP. Purpose: The aims of this study is to investigate the prevalence of prostatic lesions and its PSA level among patients in anatomical pathology installation in RSUD Dr. Soetomo from year 2014 to 2016. Method: This research is a retrospective study of prostatic lesions that were conducted from year 2014 to 2016 (3 years) with emphasis on GS and PSA levels. Result: The distribution of histopathological lesion found are benign lesion, benign prostate hyperplasia, adenocarcinoma, prostatitis, benign prostate hyperplasia with prostatitis, prostatic intraepithelial neoplasia, non-Hodgkin lymphoma, sarcoma, transitional cell carcinoma, and squamous cell carcinoma. The most common findings in this cohort is benign lesion (34.6%) with age group of 61-70 years old (51.94%) and adenocarcinoma with high GS of 9 (60%). Meanwhile, patients with GS ­8 (high risk patient) contributed for 84.8%. Most of the cases (69.2%) have elevated PSA level of 20 ng/ml. Conclusion: The prevalence of prostatic lesions were able to be determined in different age groups. High GS indicates a more aggressive type of adenocarcinoma suffered, high risk for CaP.  The results show that the possibility to detect malignancy with rising PSA level are higher, although PSA is not considered as a specific marker.Keywords: prostate, histopathology, benign lesion, PSA.
Perbedaan Ekspresi E-cadherin dan MMP-9 pada Adeno-karsinoma Prostat Bermetastasis dan Non Metastasis ke Tulang Aniek Meidy Utami; Sjahjenny Mustokoweni; Anny Setijo Rahaju
Majalah Patologi Indonesia Vol 25 No 1 (2016): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.592 KB)

Abstract

Latar belakang Kanker prostat merupakan keganasan yang paling banyak dijumpai pada laki-laki di Amerika Serikat, dengan jumlah sekitar 29% dari semua kanker dan menjadi penyebab 10% kematian di populasi 15% di antaranya sudah memiliki metastasis saat diagnosis. E-cadherin berperan penting dalam adhesi antar sel epitel dan mekanisme arsitekstur jaringan sedangkan MMP-9 berperan dalam menciptakan lintasan untuk migrasi sel tumor. E-cadherin dan MMP-9 diduga sebagai molekul utama yang berperan dalam proses invasi dan metastasis. Tujuan penelitian ini untuk membuktikan adanya peran E-cadherin dan MMP-9 dalam proses metastasis tulang pada adenokarsinoma prostat. Metode Penelitian ini menggunakan desain potong lintang terhadap sediaan adenokarsinoma prostat berasal dari prostatektomi radikal dan prostatektomi transurethral di RSUD Dr. Soetomo Surabaya periode Januari 2009-Mei 2012. Kemudian dicari data bone scan untuk melihat metastasis ke tulang. Dari data tersebut dilakukan review pemeriksaan histopatologik dan imunohistokimia dengan antibodi E-cadherin dan MMP-9. Perbedaan ekspresi E-cadherin dan MMP-9 kejadian metastasis tulang dianalisis dengan uji Mann-Whittney, sedangkan hubungan antara keduanya diuji dengan Spearman. Hasil Hasil penelitian tidak menunjukkan adanya perbedaan ekspresi E-cadherin (p>0,05), menunjukkan perbedaan yang bermakna ekspresi MMP-9 pada adenokarsinoma prostat non metastasis dengan yang mengalami metastasis(p
Collecting Duct Carcinoma: A Rare Entity Ferdinant Martinus Djawa; Anny Setijo Rahaju
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 4, No 1 (2020)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (518.871 KB) | DOI: 10.30651/jqm.v4i1.3083

Abstract

ABSTRACTCollecting duct carcinoma is a rare and highly aggressive subtype of renal cell carcinoma. The incidence rate is less than 1-2% of all renal tumors and usually, affect middle-aged adult, commonly in men. We reported a 76-year-old man complains of an intermittent painless gross hematuria, abdominal mass and left flank pain for approximately three months. The CT abdomen showed a slightly enhancing solid mass in the left kidney and para-aorta lymphadenopathy. Cut surfaces of the kidney showed a solid-cystic and ill-defined greyish-white tumor. Microscopically, tumor formed solid sheets and tubulopapillary structures lined by neoplastic cells, hobnailing nuclei, abnormal mitotic, and a desmoplastic stroma with lymphoplasmacytic infiltration, and the immunochemical profile were PAX8 (+) /p63 (-). Based on these findings, the diagnosis was a collecting duct carcinoma. This tumor arising from the collecting duct of Bellini in the renal medulla, accounts for less than 1-2% of all renal masses and important to be distinguished from other tumors due to differences in prognosis and therapeutic. Histopathological examination is needed to establish the diagnosis. A case of collecting duct carcinoma that occurred in a 76-year-old man has been reported. A definitive diagnosis can only be done with a detailed histopathological examination for patient management benefits.Keywords          : renal cell carcinoma, collecting duct carcinoma, urothelial carcinoma, PAX8, p63
Analisa Ekspresi E-cadherin dan MMP-2 pada Karsinoma Sel Skuamosa Laring Tuti Andayani; Etty Hary Kusumastuti; Anny Setijo Rahaju
Majalah Patologi Indonesia Vol 27 No 3 (2018): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Abstract

Latar belakangKarsinoma laring merupakan keganasan kedua terbanyak pada kepala dan leher dan lebih dari 90% berupa karsinoma sel skuamosa (KSS). Penanganan kanker laring berkaitan dengan diagnostik dan terapi, terutama ketika penyakit ini terdiagnosis pada stadium lanjut dengan keterlibatan kelenjar getah bening (KGB). Metastasis KSS pada KGB melibatkan beberapa protein yang berperan dalam adhesi sel seperti E-cadherin dan matrix metalloproteinase2 (MMP-2). Pada penelitian ini dilakukan analisis ekspresi E-cadherin dan MMP-2 pada KSS laring untuk menilai peran kedua protein tersebut dalam proses metastasis ke KGB leher.MetodePenelitian observasional analitik dengan pendekatan cross sectional. Sampel penelitian adalah blok parafin KSS laring di Departemen Patologi Anatomik RSUD Dr. Soetomo Surabaya selama Januari 2013-Desember 2014. Tiga puluh sampel terdiri atas dua kelompok, yaitu metastasis KGB dan non metastasis KGB dan dilakukan pemeriksaan imunohistokimia dengan antibodi E-cadherin dan MMP-2. Ekspresi E-cadherin dan MMP-2 dinilai secara semi kuantitatif dan dianalisis dengan tes Mann-Whitney, sedangkan korelasi antara ekspresi E-cadherin dan MMP-2 dengan tes Spearman.HasilTidak terdapat korelasi yang signifikan antara ekspresi E-cadherin dan MMP-2 pada KSS laring. Tidak terdapat perbedaan ekspresi E-cadherin dan MMP-2 yang signifikan antara KSS laring metastasis dan non metastasis ke KGB leher.KesimpulanEkspresi E-cadherin dan MMP-2 tidak berbeda bermakna karsinoma sel akuamosa laring tanpa dan metastasis ke KGB leher. Tidak terdapat korelasi ekspresi E-cadherin dan MMP-2 pada kejadian metastasis KGB leher pada KSS laring.
Relationship of Prostate-Specific Antigen (PSA) and Prostate Volume in Patients with Biopsy Proven Benign Prostatic Hyperplasia (BPH) Aulia Nur Fadila; Anny Setijo Rahaju; Tarmono Tarmono
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 4, No 2 (2020)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v4i2.3426

Abstract

Abstract  Benign prostatic hyperplasia (BPH) is one of the most common benign tumors in men with prevalence ranging from 50% for men in their 60s to 90% for men in their 80s. The researcher sought to determine the relationship of prostate-specific antigen (PSA) and prostate volume in patients with benign prostatic hyperplasia. This study was based on 33 cases of benign prostatic hyperplasia in the Department of Urology, RSUD Dr. Soetomo Surabaya that diagnosed by histopathology examination. Cases with malignancy, acute urinary retention and prostatitis were excluded. The variables of prostate-specific antigen and prostate volume were examined. The results of this study found that 33 men were enrolled with mean PSA 16,04 ng/ml and a mean prostate volume of 49,13 ml. Overall, 84,8% had PSA level >4 ng/ml and 90,9% had prostate volume >25 ml. Prostate-specific antigen has significant correlation with prostate volume (p=0,019; r=0,362). This study concluded that prostate-specific antigen and prostate volume showed a significant correlation. Keywords             : benign prostatic hyperplasia, prostate specific antigen, prostate volumeCorrespondence   : anny_sr@fk.unair.ac.id
The Correlation of EMMPRIN and EGFR Overexpression toward Muscle Invasiveness in Urothelial Carcinoma of Bladder Leonita Agustin Hambalie; Anny Setijo Rahaju; Gondo Mastutik
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14782

Abstract

Urothelial carcinomas represent 90% of all primary bladder cancers. Muscle invasion is a critical prognosticdeterminant in urothelial carcinoma. The overexpression of EMMPRIN and EGFR was found in urothelialcarcinoma. The association between the two markers has not been reported in urothelial carcinoma,therefore we aimed to analyze the expression of EMMPRIN and EGFR and investigate their association withurothelial carcinoma invasiveness. Paraffin-embedded tissues were obtained from 54 urothelial carcinomapatients which then underwent immunohistochemistry staining for EMMPRIN and EGFR antibody. Thecomparison of EMMPRIN and EGFR expression was tested using the Mann Whitney U test. The correlationwas analyzed using the Spearman test. Results showed a significant difference of EMMPRIN expressionbetween non-muscle-invasive and muscle-invasive bladder cancer (p = 0.000), and EMMPRIN expressionwas significantly correlated with the muscle invasion (rs = 0.481, p = 0.000). A significant difference ofEGFR expression between the non-muscle-invasive and muscle-invasive bladder cancer was also found(p = 0.020), and EGFR expression was significantly correlated with the muscle invasion (rs = 0.319, p =0.019). The expression of EMMPRIN was positively correlated with EGFR in urothelial carcinoma (rs =0.322, p = 0.018). The expression of EMMPRIN and EGFR are two potential biomarkers for urothelialcarcinoma invasiveness which may be helpful to differentiate between muscle-invasive and non-muscleinvasive bladder cancer.
Penurunan Kerusakan Jaringan Paru Terinfeksi Tuberkulosis oleh Ekstrak Pegagan Melalui Peningkatan Ekspresi Tissue Inhibitor of Matrix Metalloproteinase-1 (SUPLEMENTATION OF EFFECT ANILYSIS OF CENTELLA ASIATICA EXTRACT IN REDUCE LUNG TUBERCULOSIS TISSUE D Arifa Mustika; Anny Setijo Rahaju; Roostantia Indrawati
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.428 KB)

Abstract

Centella asiatica is a medicinal plant used for wound healing through increasing of collagen synthesis.This evidence generates a new expectation that it could be used for therapy of tuberculosis infection,especially for healing lung tissue damage. Until now, the effects and mechanisms onC. asiatica to cure thelung tissue damage due to M. tuberculosis infection remains unclear. The aim of this study was to prove theeffect and mechanism of ethanol extract of C. asiatica to repair the rats lung tissue damaged throughexpression of the enzimmatrix metalloproteinase-1(MMP-1)danenzimtissue inhibitor of matrixmetalloproteinase-1 (TIMP-1). The study was conducted in male rats. Twenty four rats were infected withM. tuberculosis through intratrachea and randomly divided into four groups. Group 1, 2 and 3 were thetreatment groups that they were given the ethanol extract of C. asiatica at dose 375mg/kgbw, 750 mg /kgbw, and 1500 mg / kgbw, orally and once a day for fourteen days. The fourth group was a control groupthat given distilled water. On day 15 rats were euthanized and lungs tissue have been taken. Evaluationof lungs tissue damage were assessed by the Dorman’s score in Hematoxylin Eosin and evaluation of the expression of MMP - 1 and TIMP 1 were performed by immunohistochemistry. Data of TIMP-1 wereanalyzed with ANOVA and data of lung tissue damage and MMP–1 were analyzed with Mann WhitneyU (á = 0.05). The results showed that there was a significant differences in the lungs tissue damagebetween the dose groups of 375 mg / kgbw and controls (p = 0.006), the dose groups at dose 750 mg / kgbwand controls (p = 0.004 ), the dose groups of 1500 mg / kgbw and controls (p = 0.043). There wasn’t asignificant difference between the treatment groups and control in the expression of MMP-1. In the expressionof TIMP – 1, there was a significant difference between the treatment group at dose of 750 mg / kg andcontrol. The conclusion of the study is the ethanol extract of C. asiatica has the ability to reduce lung tissuedamage of rats infected with M. tuberculosis, through increasesthe expression of TIMP-1
SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT ON MALE WHITE RATS KIDNEY NECROSIS Ardy Hamonangan Naibaho; Arifa Mustika; Anny Setijo Rahaju
Folia Medica Indonesiana Vol. 52 No. 1 (2016): JANUARY - MARCH 2016
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.353 KB) | DOI: 10.20473/fmi.v52i1.5212

Abstract

Singawalang leaf is used as a traditional medicine by the communities suffering from pneumonia due to TB disease. However, experiments to explain it’s toxicity are few. Therefore, this study was conducted to disclose it’s toxicity. This was an experimental laboratory study using post–test only control group design. The sampling method was simple random sampling. The animals were randomly divided into 4 (four) groups, each group consisted of 8 (eight) mice, the control group (P0) received distilled water as a solvent material. Treatment group 1 (P1) obtained ethanol extract of singawalang (Petiveria alliaceae) in low doses of 90 mg/kgbw. Treatment group 2 (P2) obtained ethanol extract of singawalang (Petiveria alliaceae) in medium doses of 180 mg/kgbw. Treatment group 3 (P3) obtained ethanol extract of singawalang (Petiveria alliaceae) in high doses of 360 mg/kgbw. Th extracts were given with sonde once a day for 30 days. Results showed that the leaf ethanol extract singawalang (Petiveria alliaceae) does not induce sub-acute necrosis effects on kidney of male white mice (Mus musculus) in doses 90 mg/kgbw, 180 mg/kgbw, and 360 mg/kgbw.
THE MUTATION STATUS OF KRAS GENE CODON 12 AND 13 IN COLORECTAL ADENOCARCINOMA (Status Mutasi Gen Kras Kodon 12 dan 13 di Adenocarcinoma Kolorektal) Gondo Mastutik; Alphania Rahniayu; Anny Setijo Rahaju; Nila Kurniasari; Reny I’tishom
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1177

Abstract

Kanker kolorektum merupakan salah satu kanker yang tersering di dunia. Target molekuler untuk pengobatan kanker kolorektumyaitu Epidermal Growth Factor Receptor (EGFR) dengan pemberian antibodi monoklonal anti-EGFR. Pemberian pengobatan ini tidakdapat memberikan efek dampak di pasien dengan status gen KRAS bentuk mutan, sehingga perlu dilakukan pemeriksaan status mutasigen KRAS. Telitian berupa deskriptif dengan pendekatan potong lintang yang bertujuan untuk mendapatkan data status mutasi genKRAS kodon 12 dan 13 di pasien adenocarcinoma colorectal. Deteksi mutasi KRAS dilakukan dengan teknik Polymerase Chain ReactionRestriction Fragment Length Polymorphism (PCR RFLP) yang dikonfirmasi dengan sekuensing. Sampel telitian adalah 30 blok parafinyang diperoleh dari Rumah Sakit Dr.Soetomo Surabaya masa waktu Januari-Desember 2013. Setelah dilakukan ekstraksi DNA terdapat21 sampel yang dapat digunakan untuk pemeriksaan lanjutan. Hasil PCR RFLP menunjukkan terdapat 7/21 mutasi pada kodon12 dan tidak terdapat mutasi gen KRAS pada kodon 13. Mutasi pada kodon 12 yaitu GGT>GCT, GGT>GGA dan GGT>GAT yangmenyebabkan perubahan asam amino Gly12Ala, Gly12Gly dan Gly12Asp. Simpulan telitian ini adalah mutasi gen KRAS kodon 12 padaadenocarcinoma colorectal di Rumah Sakit Dr. Soetomo Surabaya sebanyak 33%.