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All Journal Buletin Peternakan JPMS (Jurnal Pendidikan Matematika dan Sains) Jurnal Ilmu Ternak dan Veteriner agriTECH Indonesian Journal of Biotechnology Widyariset Livestock and Animal Research Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Widyariset
Zaenal Bachruddin
Department Of Animal Nutrition And Feed Science, Faculty Of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281
Aerospace Engineering Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Neuroscience Physics Veterinary Other

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Utilization of mixed cellulolytic microbes from termite extract, elephant faecal solution and buffalo ruminal fluid to increase in vitro digestibility of King Grass Prabowo, Agung; Padmowijoto, Soemitro; Bachruddin, Zaenal; Syukur, Abdul
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 2 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.38 KB) | DOI: 10.14334/jitv.v12i2.547

Abstract

Cellulose is a compound of plant cell walls which is difficult to be degraded because it composed of glucose monomers linked by β-(1.4)-bound. It will be hydrolysed by cellulase enzyme secreted by cellulolytic microbes. The effective digestion of cellulose needs high activity of cellulase enzyme. This research aims to increase in vitro king grass digestibility utilizing mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid. Twelve syringes contained gas test media were randomly divided into four treatments based on sources of microbe (SM), namely: S (SM: cattle ruminal fluid [S]), RGK (SM: mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid [RGK], with composition 1 : 1 : 1), S-RGK (SM: S + RGK, with composition 1:1), and TM (without given treatment microbe). Digestibility was measured using gas test method. Average of gas production treatment of S-RGK (70.2 + 0.6 ml) was higher and significantly different (P<0.01) compared to treatment of S (60.3 + 0.8 ml), RGK (40.8 + 2.3 ml), and TM (13.3 + 2.0 ml). Utilization of mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid (RGK) that combined with microbes of cattle ruminal fluid (S) could increase in vitro digestibility of king grass. Key Words: Cellulolytic Microbe, Termite Extract, Elephant Faecal, Buffalo Ruminal Fluid
Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 Harmayani, Eni; N, Nofisulastri; Bachruddin, Zaenal
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.738 KB)

Abstract

objectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extractionand characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocinactivity. Results showed that the bacteriocin activity increased during growth and reached the highest activity duringstationary phase. The maximum bacteriocin production reached after incubation of the cell for 12 h at 37oC in TGEbroth and decreased after 96 h incubation. Extraction with adsorbtion-desorbtion method could increased a specificactivity of bacteriocin. Bacteriocin from Pediococcus sp. NWD 015 is inactivated by Proteinase-K; however it is stillactive by heat treatment at 121oC for 15 min and over pH 2 – 11. Bacteriocin of Pediococcus sp. NWD 015 was effectiveagaints Enterococcus faecalis, Staphylococcus aureus, Eschericia coli, Listeria monocytogenes but not against Salmonellathypimurium. The molecular weight of bacteriocin is 4.95 kDa.Keywords : Bacteriocins, Pediococcus sp NWD 015.
Explorasi dan Studi Komposisi Botani Gulma di Perkebunan Karet PTPN IX Kebun Getas sebagai Pakan Ternak Ruminansia Harwanto, Harwanto; Suwignyo, Bambang; Bachruddin, Zaenal; Pawening, Galih
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 11 No 1 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i1.133

Abstract

Abstract Weed is one of the plants that grow around rubber plantations that have the potential to provide forage for ruminant feed. This study aimed to explore the environmental conditions and identify the botanical composition of weeds under the shade of rubber trees in immature plants (IP) at PTPN IX, Getas Farm, Semarang, Central Java. Exploratory research was conducted to identify the botanical composition of weeds in three IP groups, such as 1-2, 3-4, and 5-6 years old. The composition of the weeds’ botany was divided based on the plants’ morphology into grass, legume, forbs, and browse. The environmental conditions data were analyzed using the ANOVA method and followed by Duncan's Multiple Range Test (DMRT). The botanical composition data were tabulated using a summed dominance ratio (SDR). The results showed the light intensity, wind speed, environment temperature, and weed botanical composition decreased as the rubber plants ages. The composition of rubber plantation weeds on IP 1-2 years old consisted of 32 species (5 kinds of grass, 4 legumes, 21 forbs, and 2 browse). The composition of weeds on IP 3-4 years old consisted of 15 species (8 kinds of grass, 2 legumes, and 5 forbs), while IP 5-6 years old consisted of 6 species (4 kinds of grass, 1 legume, and 1 forb). The dominant weeds of each IP were Calopogonium mucunoides, Cyrtococcum acrescens, and Cyrtococcum oxyphyllum, respectively. Based on the results of the study it was concluded that the area of IP 1-2 years old rubber plantation weeds has the highest botanical composition and potential as ruminant feed. Keywords: Environmental conditions; Immature plants; Rubber plantation; Weed Abstrak Gulma merupakan salah satu tanaman yang tumbuh di sekitar tanaman perkebunan karet yang berpotensi sebagai penyedia hijauan pakan ternak ruminansia. Penelitian ini bertujuan untuk mengeksplorasi kondisi lingkunganpertumbuhan gulma dan mengidentifikasi komposisi botani di bawah naungan pohon karet pada tanaman belum menghasilkan (TBM), di PTPN IX, Kebun Getas. Semarang, Jawa Tengah. Penelitian dilakukan secara eksploratif terhadap kondisi lingkungan dan identifikasi komposisi botani gulma pada 3 kelompok TBM yaitu umur 1-2; 3-4; dan 5-6 tahun. Komposisi botani gulma dibagi berdasarkan morfologi tanaman yaitu rumput, legum, forb, dan browse. Data kondisi lingkungan kelompok TBM dianalisis menggunakan ANOVA dan diuji lanjut menggunakan Duncant’s Multiple Range Test (DMRT). Data dominasi komposisi botani ditabulasi menggunakan summed dominance ratio (SDR). Hasil penelitian menunjukkan intensitas cahaya, kecepatan angin, suhu lingkungan, dan komposisi botani gulma menurun seiring bertambahnya umur tanaman karet. Komposisi gulma perkebunan karet pada TBM 1-2 terdapat 32 spesies meliputi 5 rumput, 4 legum, 21 forb, dan 2 browse; TBM 3-4 terdapat 15 spesies meliputi 8 rumput, 2 legum, dan 5 forb, sedangkan TBM 5-6 terdapat 6 spesies meliputi 4 rumput, 1 legum, dan 1 forb. Gulma yang mendominasi masing-masing TBM yaitu Calopogonium mucunoides, Cyrtococcum acrescens dan Cyrtococcum oxyphyllum. Berdasarkan hasil penelitian disimpulkan gulma perkebunan karet lahan TBM 1–2 memiliki komposisi botani dan potensi tertinggi sebagai pakan ternak ruminansia. Kata kunci: Gulma; Kondisi lingkungan; Perkebunan Karet; TBM
Utilization of mixed cellulolytic microbes from termite extract, elephant faecal solution and buffalo ruminal fluid to increase in vitro digestibility of King Grass Agung Prabowo; Soemitro Padmowijoto; Zaenal Bachruddin; Abdul Syukur
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.38 KB) | DOI: 10.14334/jitv.v12i2.547

Abstract

Cellulose is a compound of plant cell walls which is difficult to be degraded because it composed of glucose monomers linked by β-(1.4)-bound. It will be hydrolysed by cellulase enzyme secreted by cellulolytic microbes. The effective digestion of cellulose needs high activity of cellulase enzyme. This research aims to increase in vitro king grass digestibility utilizing mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid. Twelve syringes contained gas test media were randomly divided into four treatments based on sources of microbe (SM), namely: S (SM: cattle ruminal fluid [S]), RGK (SM: mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid [RGK], with composition 1 : 1 : 1), S-RGK (SM: S + RGK, with composition 1:1), and TM (without given treatment microbe). Digestibility was measured using gas test method. Average of gas production treatment of S-RGK (70.2 + 0.6 ml) was higher and significantly different (P<0.01) compared to treatment of S (60.3 + 0.8 ml), RGK (40.8 + 2.3 ml), and TM (13.3 + 2.0 ml). Utilization of mixed cellulolytic microbes of termite extract, elephant faecal solution, and buffalo ruminal fluid (RGK) that combined with microbes of cattle ruminal fluid (S) could increase in vitro digestibility of king grass. Key Words: Cellulolytic Microbe, Termite Extract, Elephant Faecal, Buffalo Ruminal Fluid
Fermentasi Onggok Menggunakan Mutan Trichoderma untuk Produksi Selulase Ali Mursyid Wahyu Mulyono; Muhammad Nur Cahyanto; Zuprizal Zuprizal; Zaenal Bachruddin
agriTECH Vol 29, No 2 (2009)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.18 KB) | DOI: 10.22146/agritech.9763

Abstract

The objective of the research was to study the influence of initial a of medium, inoculum concentration, initial pH of the medium and incubation time during fermentation of cassava bagasse by mutant Trichoderma AA1 on cellulase production. Fermentation of cassava bagasse was carried out by solid substrate fermentation method. The medium was inoculated by Trichoderma AA1 and incubated for four days. The initial a of medium (0.96, 0.97, 0.98, and 0.99), inoculum concentration (105, 106, 107, dan 108 spores/g), and initial pH of the medium (4.5, 5.0, 5.5, and 6.0) were stud- ied by measuring the cellulase activity during fermentation. The production of cellulase was the best when the medium had initial a of 0.99, inoculum concentration of 107 spores/g, and initial pH of 5. The peak of the cellulase production was achieved after 3-days fermentation. The cellulase activities obtained were 0.168 and 0.072 µmol/minute/ml forcarboxy methyl cellulase dan filter paper-ase respectively.ABSTRAKPenelitian ini bertujuan mempelajari pengaruh a awal medium, konsentrasi inokulum, pH awal, dan waktu fermentasi onggok menggunakan mutan Trichoderma AA1 terhadap produksi selulase. Fermentasi onggok menggunakan metode fermentasi substrat padat. Medium diinokulasi dengan mutan Trichoderma AA1 dan diinkubasikan selama 4 hari. Variabel yang dipelajari meliputi a awal medium (0,96; 0,97; 0,98; dan 0,99), konsentrasi inokulum (105, 106, 107, dan 108  spora/g), dan pH awal medium (4,5, 5,0, 5,5, dan 6,0.). Hasil penelitian menunjukkan bahwa kondisi terbaik fermentasi onggok menggunakan mutan Trichoderma AA1 untuk menghasilkan selulase adalah: a awal medium 0,99, konsentrasi inokulum 107 spora/g, pH awal medium 5, dan waktu fermentasi 3 hari. Aktivitas selulase yang dihasilkan adalah 0,168 dan 0,072 µmol/menit/ml masing-masing untuk carboxy methyl cellulase dan filter paper-ase.
Production and Extraction Of Antibacterial Bacteriocin from Pediococcus sp. NWD 015 N. Nofisulastri; Zaenal Bachruddin; Eni Harmayani
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.738 KB) | DOI: 10.22146/ijbiotech.7566

Abstract

objectives were to study the growth pattern of Pediococcus sp. NWD 015 and bacteriocin activity, extractionand characterization of bacteriocin, and to determine the effect of storage time and temperature on bacteriocinactivity. Results showed that the bacteriocin activity increased during growth and reached the highest activity duringstationary phase. The maximum bacteriocin production reached after incubation of the cell for 12 h at 37oC in TGEbroth and decreased after 96 h incubation. Extraction with adsorbtion-desorbtion method could increased a specificactivity of bacteriocin. Bacteriocin from Pediococcus sp. NWD 015 is inactivated by Proteinase-K; however it is stillactive by heat treatment at 121oC for 15 min and over pH 2 – 11. Bacteriocin of Pediococcus sp. NWD 015 was effectiveagaints Enterococcus faecalis, Staphylococcus aureus, Eschericia coli, Listeria monocytogenes but not against Salmonellathypimurium. The molecular weight of bacteriocin is 4.95 kDa.Keywords : Bacteriocins, Pediococcus sp NWD 015.
Optimalisasi Pengikatan Tanin Daun Nangka dengan Protein Bovine Serum Albumin (Optimalisation Binding of Jackfruit Leaves Tannin with Bovine Serum Albumin Protein) Wahidin Teguh Sasongko; Lies Mira Yusiati; Zaenal Bachruddin; Mugiono (Mugiono)
Buletin Peternakan Vol 34, No 3 (2010): Buletin Peternakan Vol. 34 (3) Oktober 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v34i3.84

Abstract

Tannins are high molecular weight polyphenol compounds with ability to bind proteins. Based on the structure,albumin are simple globular molecule protein. Optimalisation binding of jackfruit leave tannins to bovine serum (BSA)albumin was done in two stages. The first stage was to determine levels of tannins and condensed tannins in jackfruit leaves grown in mediterranean soil types. Second research was optimalisation binding of jackfruit leaf tannins withbovine serum albumin. In the second stage there was the determination of protein-percipitable phenolics compound and protein content using Lowry method. The data showed total phenol content was 10.63%, total tannin was 7.08%, andcondensed tannins 5.57%. The optimum capacity phenolic compound to bind protein of bovine serum albumin was 5.71+0.18 mg BSA/100 mg dry matter of jackfruit leaf, while 1 g tannin could bind 23.149 g BSA or 1 g condensedtannin could bind 28.885 g BSA.(Key words: Tannin, Total phenol, Total tannin, Condensed tannin, Bovine serum albumin)
Karakteristik Bakteri Asam Laktat NWD015 Hasil Isolasi dari Feses Pedet dan Pengaruh Bakteriosin terhadap Bakteri Patogen Nafiatul Umami; Zaenal Bachruddin; Lies Mira Yusiati
Buletin Peternakan Vol 30, No 1 (2006): Buletin Peternakan Vol. 30 (1) Februari 2006
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v30i1.1190

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Aktivitas Enzim Carboxy Methil Cellulase dan Produksi Volatile Fatty Acid pada Fermentasi Selulosa oleh Mikrobia Rumen Secara In Vitro Nafiatul Umami; Zaenal Bachruddin; Hari Hartadi
Buletin Peternakan Vol 30, No 2 (2006): Buletin Peternakan Vol. 30 (2) Mei 2006
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v30i2.1196

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Pemanfaatan Feses Kambing sebagai Sumber Mikrobia dan Enzim Mikrobia Pengganti Cairan Rumen Ristanto Utomo; Zaenal Bachruddin; Budi Prasetyo Widyobroto; Mohamad Soejono; Risa Antari
Buletin Peternakan Vol 30, No 3 (2006): Buletin Peternakan Vol. 30 (3) Agustus 2006
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v30i3.1201

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Co-Authors Abdul Syukur Abdul Syukur Abdul Syukur Adi M.P. Nuhriawangsa Agung Prabowo Agung Prabowo Agung Prabowo Ali Mursyid Wahyu Mulyono Ali Wibowo Astuti Astuti Bambang Suwignyo Budi Prasetyo Widyobroto Chusnul Hanim Edi Suryanto Edy Permadi Engkus Ainul Yakin Eni Harmayani Eni Harmayani Galih Pawening Hari Hartadi Harwanto Harwanto Harwanto Harwanto Jamhari Jamhari Lies Mira Yusiati Mohamad Soejono Mugiono (Mugiono) Muhammad Askari Zakariah Muhammad Nur Cahyanto N. Nofisulastri Nafiatul Umami Nofisulastri N, Nofisulastri Pawening, Galih Rahma Fitriastuti Ria Millati Risa Antari Ristanto Utomo Ristianto Utomo Ristianto Utomo Ristianto Utomo Sajidan Sajidan Soemitro Padmowijoto Soemitro Padmowijoto Soemitro Padmowijoto Supadmo Supadmo Vian Dwi Chalisty Wahidin Teguh Sasongko Widayati, Okti Yuny Erwanto Zuprizal (Zuprizal)