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Studies on Dormancy Periods and Growth Rhythm of Shoot and Root of Mangosteen (Garcinia mangostana L.) Ramdan Hidayat; Achmad Surkati; Roedhy Poerwanto; Latifah K. Darusman; Bambang S. Purwoko
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.163 KB) | DOI: 10.24831/jai.v33i2.1516

Abstract

Mangosteen has a good prospectus for international market. Many people in the world like this fruit because it is delicious, has high nutrient contents, and it can be consumed as a fresh fruit. The growth of mangosteen is very slow with a very long juvenile period because of its long dormancy period. The objective of this research was to study the dormancy periods and growth rhytm of shoot and root of young and adult mangosteen seedlings. The research used completely randomized design and consisted of two experiments. The first experiment was to study the dormancy and flushing periods. The second experiment was to study the growth patterns of shoot and root foom several ages of mangosteen seedlings. This research was conducted at Mekarsari Fruit Park, Cileungsi, Bogor. The result of the experiment showed that two years old of mangosteen seedling had 5 flushes per year, four years old of mangosteen had 3-4 flushes per year, and eight years old of mangosteen only had 2 flushes. Dormancy periods were significantly different between 2,4 and 8 year of mangosteen seedling. After the seedlings have branches, the dormancy periods become twice as many as that before branching. The roots grew rapidly two weeks before flush and slower after flush occurred.   Key words: Garcinia mangostana, dormancy, flush, seedling
Kandungan Beberapa Zat Endogen pada Buah Retensi dan Buah akan Rontok pada Mangga Sakhidin Sakhidin; Bambang S. Purwoko; Sudirman Yahya; R. Poerwanto; Slamet Susanto; A. S. Abidin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 34 No. 2 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.843 KB) | DOI: 10.24831/jai.v34i2.1287

Abstract

The aim of this research was to study the relationship between fruit drop  and contents of auxin, polyamines, ACC, total sugar, and starch. Cultivars of mango used were Gadung 21 and Lalijiwo.The persisting fruit and abscising fruits were used to quantify auxin, polyamine, ACC, total sugar, and starch.  The content of polyamine and ACC was determined on pericarp, auxin on seed, whereas the content of starch and total sugar was determined on mesocarp. The result of research showed that fruit drop was preceded by low content of auxin that increased the sensitivity of abscission zone to ethylene.  The increased sensitivity of abscission zone to ethylene was supported by low content of polyamine and  high content of ACC. The abscising fruit has high content of starch, but low in  total sugar content.   Key words : Mango, persisting fruit, abscising fruit, auxin, polyamine, ACC, total sugar, starch
Evaluasi Pertumbuhan dan Produksi Padi Gogo Haploid Ganda Toleran Naungan dalam Sistem Tumpang sari Priatna Sasmita; Bambang S. Purwoko; S. Sujiprihati; I. Hanarida; I. S. Dewi; M. A. Chozin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 34 No. 2 (2006): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.05 KB) | DOI: 10.24831/jai.v34i2.1283

Abstract

Three doubled haploid (DH) upland rice lines obtained from anther culture having good agronomic and shade  tolerant traits were planted in an intercropping with maize. Their  monoculture systems were also included.  Those lines were GI-8, IG-19, and IW-56. Jatiluhur was also treated similar as shade tolerant control cultivar. The experiment was arranged in randomized complete block design with three replications. The treatments consisted of eight intercropping systems, four monoculture systems of rice, and two monoculture systems of maize. The result showed that all of tested DH lines were consistently shade tolerant under intercropping condition. The characteristics was represented by  similar growth and grain yield with  Jatiluhur under  intercroping system. The highest grain yield was 2.49 t/ha resulted by GI-8 and IG-19 lines  at the time that Jatiluhur was 2.36 t/ha. The intercropping of DH lines with  maize also showed the Land Equivalent Ratio (LER) =1.33-1.58, while Jatiluhur LER = 1.25-1.28, indicating that agronomically they were more advantageous than those in the monoculture system.   Key words :  Doubled haploid,, intercropping system, shade tolerant lines
Pengaruh Alelopati Jarak Pagar (Jatropha curcas L.) terhadap Perkecambahan Benih Jagung, Tomat dan Padi Gogo Ince Raden; Bambang S. Purwoko; Edi Santosa; , Hariyadi; Munif Ghulamahdi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 1 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.651 KB) | DOI: 10.24831/jai.v36i1.1358

Abstract

  The objective of this research was to evaluate the effect of extract of leaves, seed and root of physic nut on the germination of corn, tomato, and upland rice. Research was conducted by using Complete Randomized Block Design with two factors, i.e., plant organ (leaves, seed and root) and species (corn, tomato and upland rice). The results showed that extract of 36 g dried organ/200 ml water (0.18 g/ml water) affected seed germination of corn, tomato, and upland rice. Extract of leaves tended to reduce percentage of corn seed germination (55.30% vs 70.68%), and extract of root to reduce germination of tomato and upland rice, 52.70% and 68.67%, compared to control 76% and 84%, respectively.   Key words:  Plant organ extract, physic nut, germination  
Pengaruh Tinggi Pangkasan Batang Utama dan Jumlah Cabang Primer yang Dipelihara terhadap Produksi Minyak Jarak Pagar (Jatropha curcas L.) Ince Raden; Bambang S. Purwoko; , Hariyadi; Munif Ghulamahdi; Edi Santosa
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 37 No. 2 (2009): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (92.721 KB) | DOI: 10.24831/jai.v37i2.1409

Abstract

The objective of this research was to study shoot architecture in relation to growth, increase production and seed oil content through various stem pruning and number of primary branches. This research was conducted using Randomized Complete Block Design with single factor. It consisted of ten treatments, i.e., K=kontrol, T20= height of stem pruning at 20 cm from soil and without control on number of primary branches, T20-2=height of stem pruning at 20 cm from soil and two primary branches, T20-3= height of stem pruning at 20 cm and three primary branches, T30= height of stem pruning at 30 cm and without control on number of primary branches, T30-2= height of stem pruning at 30 cm and two primary branches, T30-3=height of stem pruning 30 cm and three primary branches, T40=height of stem pruning at 40 cm and without control on number of  primary branches, T40-2=height of stem pruning at 40 cm and two primary branches, and T40-3=height of stem pruning 40 cm and three primary branches. The results showed that stem pruning increased number of primary branches. The highest seed oil content and seed production was achieved T40 and T30-3 treatments i.e., 323.81 g/plant or 0.810 ton/ha and 320.61 g/plant or 0.802 ton/ha with seed oil yield 244.56 kg/ha and 276.61 kg/ha, respectively.   Key words : Jatropha curcas L., seed oil content, pruning, primary branches
Eliminasi Onion yellow dwarf virus melalui Kultur Meristem Tip pada Bawang Merah (Allium ascalonicum L.) Aqlima ,; Bambang S. Purwoko; Sri Hendrastuti Hidayat; Diny Dinarti
Jurnal Hortikultura Indonesia Vol. 8 No. 1 (2017): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.098 KB) | DOI: 10.29244/jhi.8.1.22-30

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ABSTRACTMeristem tip culture is culture of isolated meristem with 1-2 leaf primordia on suitable medium. This method is generally used to obtain free virus plant. Optimation of plant growth regulators (PGRs) was done to accelerate explant growth without callus formation and to avoid somaclonal variation in meristem tip culture. The aims of this study were to achieve the best combination of PGR for meristem tip growth and to evaluate meristem tip culture potential for Onion yellow dwarf virus (OYDV) elimination in shallot. This study used combination of PGRs 0.25 mg L-1 (2-ip, BAP, GA3, kinetin) with or without 0.1 mg L-1 IAA and medium without PGR. This research consisted of two experiments conducted separately. In experiment I, cv. Bima Brebes was used and experiment II cv. Tiron was used. Each experiment was arranged in completely randomized block design with single factor (PGR combination) that has 8 combination levels and 3 replications. The result showed that medium without PGR was the most efficient for meristem tip growth. Primary shoot was growing without callus formation. RT-PCR analysis showed that all of the tested samples were still infected by OYDV. Meristem tip culture method did not eliminate OYDV in both cultivars.Keywords: Auxin, cytokinin, GA3, OYDV, RT-PCRABSTRAKKultur meristem tip merupakan kultur meristem yang diisolasi 1-2 primordia daun dan pada media yang sesuai. Metode ini umum digunakan untuk mendapatkan tanaman bebas virus. Optimasi terhadap zat pengatur tumbuh (ZPT) dilakukan untuk mempercepat pertumbuhan eksplan tanpa disertai pembentukan kalus untuk menghindari terjadinya variasi somaklonal pada kultur meristem tip. Penelitian ini bertujuan untuk mendapatkan kombinasi ZPT terbaik bagi pertumbuhan meristem tip dan untuk mengevaluasi potensi kultur meristem tip dalam mengeliminasi virus Onion yellow dwarf virus (OYDV) pada tanaman bawang merah. Penelitian ini menggunakan 0.25 mg L-1 (2-ip, BAP, GA3, kinetin) dengan penambahan atau tanpa 0.1 mg L-1 IAA serta media tanpa ZPT. Penelitian ini terdiri atas 2 percobaan terpisah. Percobaan 1 menggunakan cv. Bima Brebes dan Percobaan 2 menggunakan cv. Tiron. Masing-masing percobaan disusun berdasarkan rancangan kelompok lengkap teracak (RKLT) dengan 1 faktor, yaitu kombinasi ZPT yang terdiri atas 8 taraf kombinasi dan 3 ulangan. Hasil yang didapat menunjukkan bahwa media tanpa penambahan ZPTmerupakan media yang paling efisien untuk pertumbuhan tunas meristem tip. Tunas utama tumbuh tanpa disertai pembentukan kalus. Hasil analisis RT-PCR menunjukkan bahwa seluruh sampel yang dideteksi masih terinfeksi OYDV. Metode kultur meristem tip belum dapat mengeliminasi virus OYDV pada kedua kultivar bawang merah.Kata kunci: Auksin, GA3, OYDV, RT-PCR, sitokinin
Potensi Hasil Jarak Pagar (Jatropha curcas L.) pada Satu Tahun Budidaya di Lahan Kering Lombok Barat, Nusa Tenggara Barat Bambang B. Santoso; , Hasnam; , Hariyadi3; Slamet Susanto; Bambang S. Purwoko
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 2 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.866 KB) | DOI: 10.24831/jai.v36i2.20510

Abstract

Productivity of Physic Nut (Jatropha curcas L.) depends on environment and culture intensity. We evaluated yield potential of Jatropha curcas L. at dry land (sandy entisol soil type) of West Lombok, West Nusa Tenggara using three types of propagules, i.e., stem cutting, seed, and seed after pruning and were designed with Randomized Block Design in three replications during November 2006 until November 2007. Result showed that productivity of nut is 880.78 kg/ha (352.31 g/plant) from stem cutting, 749.81 kg/ha (299.92 g/plant) from seed, and 484.11 kg/ha (193.64 g/plant) from seed followed by pruning after 2 weeks planting.
Pewarisan Sifat Toleransi Padi Sawah (Oryza sativa L) Terhadap Cekaman Suhu Rendah Yusuf L. Limbongan; Hajrial Aswidinnoor; Bambang S. Purwoko; , Trikoesoemaningtyas2
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 2 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.701 KB) | DOI: 10.24831/jai.v36i2.20482

Abstract

One of the definite successful of rice breeding programs for increasing yield and tolerance to environmental stress condition is selection method. Selection method will be effectively if it is supported by a perfect knowledge of genetics character inheritance. The objectives of this study is to investigate the information of inheritance pattern adapted of rice towards low temperature stress condition. The experiment was conducted in both Sesean rural area, Toraja regency, South Sulawesi on 1500 m above sea levels, an mean temperature 15 o C, type of soil is Podzolic and at Bogor Agriculture Institute. This experiment is done in January 2006 until July 2007. The result showed that tolerance parental grew well and higher yield than sensitive parental. There were no significant differences between F1 and F1 reciprocal for all characters. Tolerance parental is more superior than sensitive parental in all characters have been tested. Mean of F1 generation is in between tolerance parental and sensitive parental. BCP1 has spheres wider than parentals and F1 generation, but BCP2 smaller than parentals and F1 generation. F2 generation is spheres wider than the average of both parentals. The m [d] [h] genetic model is the most suitable for all characters. Except, date of flowering and weight of filled rice where appropriate models is m [d] [h] [l]. Broad sense and narrow sense heritability is range between low to high. Based on the degree of dominant, additive effect is higher than dominant effect. The negative F value reached on date of flowering and fur lenght; and others characters, F value is positive.
Efek Suhu Simpan dan Pelapis terhadap Perubahan Kualitas Buah Pisang Cavendish Bambang S. Purwoko; K. Suryana
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 28 No. 3 (2000): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1620.499 KB) | DOI: 10.24831/jai.v28i3.1557

Abstract

The objective of the experiment was to determine the effect of coating materials and storage temperatures in inhibiting the ripening process of banana fruits. The experiment consisted of two factors namely storage temperatures (ambient and cool) and coating materials (control, Carnauba wax, bee wax, and Sempefresh). One experimental unit consisted of two fingers of banana. The experiment was replicated three times. Observation was conducted on the following variables: peel color development, weight loss, fruit softening, ratio of flesh and peel weight, sugar content, and titratable acidity. Result of the experiment showed that carnauba wax 6 % inhibited weight loss, fruit softening, and the increase of flesh and peel weight ratio. Bee wax inhibited fruit softening, peel color development and sugar content. Fruit treated with carnauba wax 6 % could be stored at cool temperature for 25.9 days, 10.5 days longer than untreated fruit stored at ambient temperature. Among the three coating materials, Sempefresh had the lowest effectiveness in inhibiting bananaf ruit ripening process.   Key words: Coating, Ripening, Banana, Shelf life
Induksi Kalus dan Regenerasi Tiga Genotipe Tomat (Solanum lycopersicon L.) melalui Kultur Antera Ratna Ningsih; Bambang S. Purwoko; Muhamad Syukur; Iswari S. Dewi
Jurnal Hortikultura Indonesia Vol. 7 No. 2 (2016): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.486 KB) | DOI: 10.29244/jhi.7.2.75-82

Abstract

ABSTRACTThe aims of this research were to evaluate culture ability of 3 tomato genotypes through their androgenic response in callus induction and regeneration media. Completely randomized design with factorial arrangement and 5 replications were used. Treatments consisted of three genotypes (Tora, Ratna and hybrid variety Permata), six callus induction media in the first phase and three genotypes and two regeneration media. The result showed that hybrid variety Permata had the highest anther culture ability then others genotypes. Permata had the highest percentage of callus induction (27%) followed by Tora (14%) and Ratna (12%). The highest percentage of callus induction was shown in DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA media (39.7%) followed by DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA media (33.0%). Both genotypes and media gave low percentage of shoot induction. The percentage of shoot induction in hybrid variety Permata was 4.2% while in Tora was 2.1% and Ratna was 0%. The percentage of shoot induction in MS + 25 mg L-1 Zeatin was 2.8% while in MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA was 1.4%.Keywords: androgenesis, auxin, cytokinine, in vitro, medium, tomato ABSTRAKPenelitian ini bertujuan untuk mengetahui daya kultur antera tiga genotipe tomat melalui percobaan induksi pembentukan kalus dan regenerasi tunas. Percobaan dirancang menggunakan Rancangan Acak Lengkap faktorial dengan lima ulangan. Bahan tanam yang digunakan ialah tomat varietas Tora, Ratna dan varietas hibrida Permata. Media yang digunakan adalah 6 media induksi kalus dan 2 media regenerasi tunas. Hasil penelitian menunjukkan bahwa tomat varietas hibrida Permata memiliki daya kultur antera yang lebih baik dibandingkan genotipe lainnya. Permata memiliki persentase jumlah kalus 27% lebih tinggi dibandingkan Tora (14%) dan Ratna (12%). Media yang paling baik menginduksi kalus adalah media DBMI + 5 mg L-1 Kinetin + 2 mg L-1 NAA (39%) dan DBMII + 1 mg L-1 Kinetin + 2 mg L-1 NAA (33%). Baik genotipe maupun media yang digunakan menghasilkan jumlah tunas yang rendah. Persentase induksi tunas varietas hibrida Permata 4.2% lebih tinggi dibandingkan Tora (2.1%) dan Ratna (0%). Persentase induksi tunas media MS + 0.25 mg L-1 Zeatin (2.8%) lebih tinggi dibandingkan media MS + 1 mg L-1 Zeatin + 0.125 mg L-1 IAA (1.4%).Kata kunci: androgenesis, auksin, in vitro, media, sitokinin, tomat