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Molecular characterization of six sub population Indonesian local goats based on mitochondrial DNA D-loop Batubara, Aron; Noor, R.R.; Farajallah, A.; Tiesnamurti, B.; Doloksaribu, M.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.805 KB) | DOI: 10.14334/jitv.v16i1.634

Abstract

Indonesian local goats were spread in some region, but there was still limited data’s known about the characteristics of its genetic diversity and origin. The Mitochondrial DNA D-loop sequences were used to study the genetic diversity and relationships of six sub population Indonesian local goats, namely, Kacang, Marica, Samosir, Jawarandu, Muara and Bengali goats. From 539 blood samples and DNA extraction collections were selected about 60 samples (10 samples each sub populations) analyzed by PCR-RFLP methods, followed sequence analyzed about 5 PCR products each sub population. The results of the sequence analyses were edited and acquired about 957 bp of nucleotides length. After the alignment analyses were found 50 polymorphic sites which divided into 19 haplotype groups of mtDNA D-loop region. The value of nucleotide diversity was 0.014 ± 0.002. Analysis of Neighbour Joining with Kimura 2 Parameter methods and bootstrap test with 1000 replication indicated that each sub population groups was significantly different between one groups to the others. The maternal lineages origin of six breeds of Indonesian local goats was included to the group of lineage B. The Lineage B was the maternal origin of the haplogroup of goats in the region of East Asia, South Asia, China, Mongolia, North and South Africa, Malaysia, Indonesia, Pakistan and India. Key words: Genetic Diversity, mtDNA D-loop, Haplotypes, Local Goats
Cryopreservation of Boer goat spermatozoa: Comparison of two freezing extenders based on post-thaw sperm quality and fertility rates ., Anwar; Pamungkas, Fitra Aji; Batubara, Aron
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 2 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.554 KB) | DOI: 10.14334/jitv.v19i2.1041

Abstract

Boer goat have recently been popularly used for cross breeding with local goats. However, it is currently considered a breed at very limited number with relatively high prices . In this context, the cryopreservation of spermatozoa is important because it could be conserved for a very long period of time. Egg yolk extenders are most commonly used for cryopreservation of goat sperm. The aim of this study was to compare the ability of two extenders to maintain sperm viability after cryopreservation. Semen from three male Boer goat aged about 2-3 years old was collected using artificial vagina and frozen with Tris and Triladyl extender. The results showed that percentage of motility, viability and membrane integrity of spermatozoa with Tris and Triladyl extenders at every stage of cryopreservation showed not significantly difference (P>0.05), except the percentage of sperm motility post thawing of Triladyl was higher than Tris extender (52.00±4.47% vs 47.50±2.74%, P<0.05). Cryopreserved semen in Tris extender provided the same fertility rates after cervical insemination compared to Triladyl (62.50% vs 60.00%). In conclusion, the Tris extender has the same capabilities to Triladyl in cryopreservation of Boer goat spermatozoa as to maintain sperm quality and fertility rates. Key Words: Boer Goat, Spermatozoa, Cryopreservation, Fertility 
Responses of two genotypes of lambs on the infection of Haemonchus contortus and the level of energy supplements Ginting, Simon P; Batubara, Aron; Romjali, Endang; Rangkuti, M; ., Subandriyo
Indonesian Journal of Animal and Veterinary Sciences Vol 4, No 1 (1999)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.216 KB) | DOI: 10.14334/jitv.v4i1.133

Abstract

A study was conducted to evaluate the responses of lambs on the infection of Haemonchus contortus and the level of energy supplements. Forty eight male lambs, 3-4 months old were used in a factorial experimental design (2x2x2) which lasted for 9 weeks. The factors were breed (Local Sumatra and Sungai Putih: 50% Local Sumatra, 25% St. Croix; 25% Barbados Blackbelly), level of Haemonchus infection (3,000 L3 and 1,500 L3 per week) and level of energy supplement (2,900 kcal/kg DM and 2,300 kcal/kg DM). Infection at 3,000 L3 decreased (P<0.05) ADG of lambs of both energy levels. An extremely low ADG (10-16 g) was observed in 3000 L3 group given low energy level. Body condition of lambs fed high energy level was constant over the experiment, but it decreased at the rate of 0.9 to 1.1 unit at the low energy supplement. EPG was higher (P>0.001) in the 3000 L3 group, was lower (P<0.05) in the Local Sumatra, but not affected (P>0.10) by energy level. PCV was not affected (P>0.10) by infection levels, but lower (P<0.01) in the low energy group or in the St Croix crosses. Eosinophil and serum protein concentration were not affected by energy and infection level, but they were higher (P<0.01) in the local Sumatra. It was concluded that strong effects of level of infection and genotype was detected to influence the impact of Haemonchus infection in lambs. However, the effect of energy levels was less consistent as judged from the EPG, PCV, eosinophil and serum protein data.   Key words : Energy level, genotype, infection level, Haemonchus contortus, lambs
Co-Authors ACHMAD FARAJALLAH Anwar . B. Tiesnamurti Endang Romjali Fitra Aji Pamungkas M Rangkuti M. Doloksaribu R.R. Noor Simon P Ginting Subandriyo .