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Antibacterial Screening of Mangrove Extract Library Showed Potential Activity against Escherichia coli and Staphylococcus aureus Audah, Kholis Abdurachim; Batubara, Razethy; Julkipli, Julkipli; Wijaya, Elza; Kurniawaty, Evi; Batubara, Irmanida
Journal of Tropical Life Science Vol 10, No 2 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.02.03

Humans are at a continuous battle against different types of diseases, so that extraordinary effort to accelerate drug discovery has become a necessity. Indonesian biodiversity is abundant natural resources that can be utilized as potential drug sources. Mangroves are among potential plant medicines that grow nearly at all Indonesian coastlines. The aim of this study was to evaluate the potential of mangrove extracts (extract library) as antibacterial agents. In this study, eight mangroves species with 16 samples from different parts of the plants such as leaf, bark or root were collected. Four types of solvents with different polarity, namely water, ethanol, ethyl acetate and hexane were used in maceration of samples producing 64 extracts. Disk diffusion method was used for antibacterial screening using five bacterial strains. There were 37 extracts showed antibacterial potential with the lowest and the highest inhibition indexes were 0.0283 and 1.8983, respectively. The highest inhibition index was recorded for ethyl acetate extract of Bruguiera gymnorrhiza root (BgR (Ea)) against Escherichia coli. The second highest inhibition index was 0.7867 recorded water extract of Avicennia marina leaf (AmL (A)) against Staphylococcus aureus. Almost all of extract showed saponin and tannin in considerable amount. This supported the data that mangrove extracts were potential as antibacterial agents.

FORMULASI LARVASIDA NABATI BERBASIS MINYAK BIJI KAMANDRAH (Croton tiglium L.) TERSTANDAR SEBAGAI PENCEGAH PENYAKIT DEMAM BERDARAH DENGUE Winoto, Evul; Iswantini, Dyah; Batubara, Irmanida; Hadi, Upik Kesumawati
Buletin Penelitian Tanaman Rempah dan Obat Vol 24, No 2 (2013): Balai Penelitian Tanaman Rempah dan Obat
Publisher : Balittro

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ABSTRAKKamandrah (Croton tiglium L.) merupakan tanaman obat yang banyak terdapat di Kalimantan. Biji kamandrah banyak dimanfaatkan sebagai obat pencahar, racun ikan, dan pembunuh jentik nyamuk. Penelitian ini bertujuan mendapatkan formula minyak biji kamandrah untuk larvasida nabati yang efektif, aman dan mendapatkan minyak kamandrah terstandar sebagai bahan baku larvasida nabati. Analisis fisiko kimia minyak biji kamandrah hasil budidaya di Sukabumi memberikan hasil kadar air 0,33%, keasaman 0,09%, viskositas 4,1 cP, berat jenis 0,9425 g ml-1, indeks bias 1,4788 serta kadar asam lemak bebas 1,65%. Hasil uji ini lebih baik dibanding dengan tanpa budidaya dari Kalimantan dan Sukabumi. Uji menggunakan spektrofotometri menunjukkan kandungan piperine minyak biji kamandrah hasil budidaya Sukabumi sebesar 0,046%; tanpa budidaya dari Sukabumi dan Kalimantan masing-masing 0,043% dan 0,037%. Kandungan piperine berpengaruh terhadap hasil uji efikasi larva nyamuk Aedes aegypti instar III, nilai LC50 pengamatan 24 jam minyak kamandrah hasil budidaya Sukabumi sebesar 114,4 ppm, minyak kamandrah tanpa budidaya dari Sukabumi dan Kalimantan masing-masing 125,2 dan 212,9 ppm. Formulasi larvasida metode granulasi basah terhadap minyak biji kamandrah hasil budidaya Sukabumi menunjukkan, kandungan minyak kamandrah 15% dengan emulsifier gom arab memberikan hasil paling efektif dengan nilai LC50 24 jam sebesar 210,01 ppm. Uji stabilitas formula larvasida nabati minyak biji kamandrah yang disimpan pada temperatur 30, 40 dan 50oC selama 28 hari menunjukkan tidak ada perubahan fisik pada granul. Selama penyimpanan terjadi peningkatan kandungan piperine dalam formula larvasida antara 0,6-234%. Uji durabilitas formula larvasida terhadap larva A. aegypti menunjukkan penurunan potensi larvasida sampai di bawah 50% pada hari ke 12 setelah aplikasi.Kata kunci: Croton tiglium, larvasida nabati, standarisasi, formulasi

FORMULASI LARVASIDA NABATI BERBASIS MINYAK BIJI KAMANDRAH (Croton tiglium L.) TERSTANDAR SEBAGAI PENCEGAH PENYAKIT DEMAM BERDARAH DENGUE Winoto, Evul; Iswantini, Dyah; Batubara, Irmanida; Hadi, Upik Kesumawati
Buletin Penelitian Tanaman Rempah dan Obat Vol 24, No 2 (2013): Balai Penelitian Tanaman Rempah dan Obat
Publisher : Balittro

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRAKKamandrah (Croton tiglium L.) merupakan tanaman obat yang banyak terdapat di Kalimantan. Biji kamandrah banyak dimanfaatkan sebagai obat pencahar, racun ikan, dan pembunuh jentik nyamuk. Penelitian ini bertujuan mendapatkan formula minyak biji kamandrah untuk larvasida nabati yang efektif, aman dan mendapatkan minyak kamandrah terstandar sebagai bahan baku larvasida nabati. Analisis fisiko kimia minyak biji kamandrah hasil budidaya di Sukabumi memberikan hasil kadar air 0,33%, keasaman 0,09%, viskositas 4,1 cP, berat jenis 0,9425 g ml-1, indeks bias 1,4788 serta kadar asam lemak bebas 1,65%. Hasil uji ini lebih baik dibanding dengan tanpa budidaya dari Kalimantan dan Sukabumi. Uji menggunakan spektrofotometri menunjukkan kandungan piperine minyak biji kamandrah hasil budidaya Sukabumi sebesar 0,046%; tanpa budidaya dari Sukabumi dan Kalimantan masing-masing 0,043% dan 0,037%. Kandungan piperine berpengaruh terhadap hasil uji efikasi larva nyamuk Aedes aegypti instar III, nilai LC50 pengamatan 24 jam minyak kamandrah hasil budidaya Sukabumi sebesar 114,4 ppm, minyak kamandrah tanpa budidaya dari Sukabumi dan Kalimantan masing-masing 125,2 dan 212,9 ppm. Formulasi larvasida metode granulasi basah terhadap minyak biji kamandrah hasil budidaya Sukabumi menunjukkan, kandungan minyak kamandrah 15% dengan emulsifier gom arab memberikan hasil paling efektif dengan nilai LC50 24 jam sebesar 210,01 ppm. Uji stabilitas formula larvasida nabati minyak biji kamandrah yang disimpan pada temperatur 30, 40 dan 50oC selama 28 hari menunjukkan tidak ada perubahan fisik pada granul. Selama penyimpanan terjadi peningkatan kandungan piperine dalam formula larvasida antara 0,6-234%. Uji durabilitas formula larvasida terhadap larva A. aegypti menunjukkan penurunan potensi larvasida sampai di bawah 50% pada hari ke 12 setelah aplikasi.Kata kunci: Croton tiglium, larvasida nabati, standarisasi, formulasi

Comparison of Immobilized Metal Affinity Chromatography Ni-NTA and Co-TALON for the Purification of Recombinant Human Erythropoietin Yana Rubiyana; Adi Santoso; Irmanida Batubara
Makara Journal of Science Vol 19, No 4 (2015): December
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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The purification of recombinant proteins is an important stage in biopharmaceutical research. A commonly used technique is immobilized metal affinity chromatography (IMAC). One of the main advantages of this type of chromatography is that the column can easily be regenerated for subsequent purification work. The mechanism of IMAC is based on bonding between metal ions immobilized on a matrix with a specific amino acid. Because of the strong interactions of the electron donor group on the imidazole ring, histidine is often used in the IMAC purification system. Two types of commercial IMAC resin use a nitrilotriacetic acid (NTA) matrix: a nickel-based (Ni-NTA) and cobalt-based (Co-NTA), better known as TALON. This study was aim to investigate the effect of the metal ions Ni2+ and Co2+ to purify recombinant human erythropoietin (rhEPO) expressed in yeast system Pichia pastoris. The results indicated that both Ni-NTA and Co-TALON gave almost the same level of protein purity; however, Ni-NTA has a higher binding affinity than Co-TALON might be due to the higher stability complex of Ni+. The average amount of protein bound by Ni-NTA and Co-TALON was 183.5 and 38.7 µg/mL, respectively.

Fraksi Alkaloid Daun Ubi Jalar Ungu (Ipomoea batatas var Ayumurasaki) Sebagai Inhibitor Î±-Glukosidase Pamungkas, Diah; Batubara, Irmanida; Suparto, Irma
Acta Pharmaciae Indonesia Vol 4 No 01 (2016): Actapharmindo
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (447.628 KB)

Ubi jalar ungu (Ipomoea batatas var Ayumurasaki) dilaporkan dapat membantu diet harian bagi penderita diabetes melitus tipe 2 karena memiliki kadar glikemik yang rendah. Salah satu mekanisme menurunkan kadar gula darah pada penderita diabetes adalah dengan menghambat enzim Î±-glukosidase. Potensi fraksi alkaloid dari daun dan fraksi antosianin dari umbi ubi jalar ungu dievaluasi sebagai inhibitor enzim Î±-glukosidase. Ubi jalar ungu yang digunakan dari Ciampea, Bogor, Jawa Barat. Fraksi alkaloid daun ubi jalar ungu pada konsentrasi 2,00% aktif menghambat kerja enzim sebesar 61,88%, sedangkan fraksi antosianin umbi ubi jalar ungu tidak aktif (0,25%). Fraksi alkaloid daun ubi jalar ungu dipisahkan lebih lanjut menggunakan kromatografi kolom dengan fase diam silika gel dan kloroformsebagai eluen. Fraksi yang mengandung alkaloid dengan semprotan Dragendorf, yakni F10, kemudian dipisahkan kembali dengan kromatografi lapis tipis preparatif. Fraksi F10.15 positif mengandung alkaloid dan dapat menghambat enzim ï¡-glukosidase sebesar 18,07% pada konsentrasi 1,25%, yang berarti pemisahan menyebabkan penurunan aktivitas. Spektrum inframerah transformasi Fourier fraksi memperlihatkan gugus fungsi âNHâ, âOH, dan âCH2â dan diduga mengandung alkaloid.

Antimicrobial and Antioxidant Activities of Resins and Essential Oil From Pine (Pinus merkusii, Pinuso ocarpa, Pinus insularis) and Agathis (Agathis loranthifolia) Tillah, Mardho; Batubara, Irmanida; Sari, Rita Kartika
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8371

The most common human pathogen that colonizes in a third of healthy people around the world are Staphylococcus aureus, and one of the materials allegedly able to overcome the pathogen is resin. Resin has been used in folk medicine for thousands of years to treat diseases. The antimicrobial activity of natural resins can be associated with a variety of organic compounds contained in them such as diterpenoids and triterpenoids. This research aimed to explore the antibacterial and antioxidant activities of Pinus merkusii, P. oocarpa, P. insularis, Agathis loranthifolia resins and essential oil. Resin was separated by distillation process to get essential oil and the residue was extracted using n-hexane, ethyl acetate (EtOAc), and methanol (MeOH). Antioxidant activity was performed by DPPH (1,1diphenyl-2-picryl hydrazyl) radical scavenging method. The antibacterial activity of resins and essential oil of the samples determined using the disc diffusion method against Staphylococcus aureus and Escherichia coli. The results showed that the yield of resin extract was ranging from 8.44 % to 95.56%. All extracts and essential oil could not inhibit E coli growth but inhibit the S. aureus growth. This experiment concluded that resin n-hexane extract from P.oocarpa was the most potent as antibacterial activity against S. aureus. All of the samples used had less potential antioxidant activity compared to positive control ascorbic acid. Result of this study show that pine resin from Indonesia has potential as an antibacterial agent.

Antibacterial and Antibiofilm Activity of Daemonorops draco Resin Wahyuni, Wulan Tri; Purwanti, Sri; Batubara, Irmanida
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13554

Daemonorops draco has been reported for its antibacterial activity and empirically used for wound healing by Anak Dalam ethnic at Jambi Province, Sumatera-Indonesia. This study was performed to evaluate antibacterial and antibiofilm activity of D. draco resin collected from Jambi. D. draco resin was extracted using n-hexane, ethyl acetate and methanol, respectively. Antibacterial activity of the extracts was evaluated using agar diffusion method against Staphylococcus aureus and Eschericia coli, whereas the minimum inhibitory concentration (MIC) and minimum bacteriacidal concentration (MBC) was determined by microdilution method. In addition, antibiofilm activity was evaluated by violet crystal method. The result showed that extraction yield of ethyl acetate was higher than methanol and n-hexane. Ethyl acetate and methanol extracts of D. draco exhibited stronger antimicrobial activity against S. aureus compare to n-hexane extract. MIC and MBC of methanol extract and chromatographic fraction (F5.1) of ethyl acetate extract were 0.5 and 1.0 mg/mL, respectively. In addition, antibiofilm assay revealed that all extracts were inhibit initial attachment of bacteria cell in biofilm formation. This result revealed a novel information that Â D. draco extracts was potential as inhibitor of biofilm formation. TLC bioautography of D. draco extracts indicated that constituent with Rf of 0.71 performed antimicrobial activity against S. aureus. This finding expected to strengthen the scientific backup for utilization of D. draco by society.

Exploration of Mango Fruits (Mangifera indica) as ?-Glucosidase Inhibitors Putri, Nadia Prilliane; Nursyamsi, Khalida Shabiba; Prayogo, Yanico Hadi; Sari, Dina Ragillia; Budiarti, Eka; Batubara, Irmanida
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10516

Mango fruit (Mangifera indica L.) is the tropical fruit that grows easily in Indonesia with plenty varieties. This study aimed to determine the varieties of mango fruit and the most potent part of mango as antidiabetic agent through ?-glucosidase inhibitory activities. Four types of mango fruit (indramayu, manalagi, harum manis, and budiraja) were used in this study. Each part of the mango fruit:peel, flesh, endosperm, and endocarp were extracted by maceration process with three different solvents (n-hexane, ethyl acetate (EtOAc), and ethanol (EtOH)). An ability of all 46 extracts in inhibiting the ?-glucosidase at a concentration of 500 ppm were determined. Then 11 extracts with the high inhibition value were determined their IC50 (concentration to inhibit 50% activity) values. EtOAc extract of manalagi, indramayu, and budi raja endosperm had the lowest IC50 value which was not statistically significantly different (at 95%) with EtOAc extract of budi raja peel. The bioautographic Thin Layer Chromatogram showed that the most active band is characterized by white luminescence under UV 366 nm, yellow color under UV 254 and visible light. The band with Rf 0.93 from EtOAc endosperm extract of indramayu and manalagi and Rf 0.73 from EtOAc budi raja peel extract are the most active band which predicted as a flavonoid. The result adds the value of the peel and seed of mango, as well as an alternative in blood sugar control, which is easy to obtain, relatively cheap, and liked by the community.

Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity Batubara, Irmanida; Kartika, Yuni; Darusman, Latifah K
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6742

The leaves of Zingiberaceae family has not been much explored its potential, especially as a skin lightening. The relationship between total anthocyanins, chlorophyll, carotenoids and tannins contents of Zingiberaceae leaves and tyrosinase activity were determined. Ten species of Zingiberaceae were separated by n-hexane and the residues were extracted with ethyl acetate. The total anthocyanins, chlorophyll, carotenoids, tannins contents and the activities of ethyl acetate extracts were determined by spectrometric method. The tyrosinase inhibition was determined by using L-tyrosine (monophenolase) and L-DOPA (diphenolase) substrates. The most active extract was the leaves extract of Zingiber purpureum which exhibited 82.86% for monophenolase inhibition and the leaves extract of Curcuma zedoaria which exhibited 90.20% for diphenolase inhibition. The correlation between carotenoids content and monophenolase inhibition was 52%, while between tannin content and diphenolase inhibition was only 15%. Therefore, among 10 leaves species of Zingiberaceae, Zingiber purpureum Roscoe and Curcuma zedoaria leaves are the most potential for tyrosinase inhibitors and can be developed as whitening agent.How to CiteBatubara, I., Kartika, Y. & Darusman, L. K. (2016). Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 371-377.

Fraksi Alkaloid Daun Ubi Jalar Ungu (Ipomoea batatas var Ayumurasaki) Sebagai Inhibitor α-Glukosidase PAMUNGKAS, DIAH DARU; BATUBARA, IRMANIDA; SUPARTO, IRMA HERAWATI
Acta Pharmaciae Indonesia Vol 4 No 2 (2016): Acta Pharmaciae indonesia Vol.4 No.2 Tahun 2016
Publisher : Pharmacy Department, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar

Ubi jalar ungu (Ipomoea batatas var Ayumurasaki) dilaporkan dapat membantu diet harian bagi penderita diabetes melitus tipe 2 karena memiliki kadar glikemik yang rendah. Salah satu mekanisme menurunkan kadar gula darah pada penderita diabetes adalah dengan menghambat enzim α-glukosidase. Potensi fraksi alkaloid dari daun dan fraksi antosianin dari umbi ubi jalar ungu dievaluasi sebagai inhibitor enzim α-glukosidase. Ubi jalar ungu yang digunakan dari Ciampea, Bogor, Jawa Barat. Fraksi alkaloid daun ubi jalar ungu pada konsentrasi 2,00% aktif menghambat kerja enzim sebesar 61,88%, sedangkan fraksi antosianin umbi ubi jalar ungu tidak aktif (0,25%). Fraksi alkaloid daun ubi jalar ungu dipisahkan lebih lanjut menggunakan kromatografi kolom dengan fase diam silika gel dan kloroform sebagai eluen. Fraksi yang mengandung alkaloid dengan semprotan Dragendorf, yakni F10, kemudian dipisahkan kembali dengan kromatografi lapis tipis preparatif. Fraksi F10.15 positif mengandung alkaloid dan dapat menghambat enzim a-glukosidase sebesar 18,07% pada konsentrasi 1,25%, yang berarti pemisahan menyebabkan penurunan aktivitas. Spektrum inframerah transformasi Fourier fraksi  memperlihatkan gugus fungsi –NH−, –OH, dan –CH2– dan diduga mengandung alkaloid.

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