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Activity of Ligninolytic Enzymes during Growth and Fruiting Body Development of White Rot Fungi Omphalina sp. and Pleurotus ostreatus HAPPY WIDIASTUTI; SUHARYANTO SUHARYANTO; AGUSTINA WULANINGTYAS; SUTAMIHARDJA SUTAMIHARDJA
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.541 KB) | DOI: 10.4308/hjb.15.4.140

Abstract

The activity of ligninolytic enzymes of white rot fungi (WRF) Omphalina sp. and Pleurotus ostreatus was observed during somatic and fruiting body development in solid substrate fermentation using empty fruit bunches of oil palm (EFB). The enzyme activity was dominated by laccase both of Omphalina sp. and P. ostreatus. The laccase activity on somatic phase (mycelium growth) was higher compared to that of fruiting body formation phase. The laccase activity of Omphalina sp. was slightly higher compared to that of P. ostreatus. The peak activity of manganese peroxidase (MnP) for Omphalina sp. was observed two weeks after inoculation, while P. ostreatus had two peaks i.e. two and four weeks after inoculation period. The MnP activity of P. ostreatus was higher compared to that of Omphalina sp. Omphalina sp. growth in EFB did not secrete lignin peroxidase (LiP) in contrast to P. ostreatus. The peak of LiP activity of P. ostreatus was reached two and four weeks after inoculation. The MnP and LiP activities declined during the development of fruiting bodies while laccase increased both in Omphalina sp. and P. ostreatus. It seems that the activities of ligninolytic enzyme profile were regulated in line with developmental phase of growth both in Omphalina sp. and P. ostreatus. Key words: ligninolytic enzyme activity, Omphalina sp., Pleurotus ostreatus, empty fruit bunches of oil palm
RESPON PERTUMBUHAN DAN HASIL TANAMAN KACANG HIJAU (Vigna radiata L.) DENGAN APLIKASI LIMBAH SAWIT DAN RHIZOBIUM DI LAHAN PASCA TAMBANG TIMAH Rahma Oktaviani; Suharyanto Suharyanto; Tri Lestari
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 23, No 3 (2020): November 2020
Publisher : Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpptp.v23n3.2020.p321-331

Abstract

Growth and Yield Responses of Mungbean (Vigna radiata L.) with Palm Oil Waste and Rhizobium Applications in Post-Tin Minning Land. Mungbean is a legume crop that has potential ability to developed in post-tin mining land. Mungbean is an adaptive plant that can survive in critical land. Organic matter and Rhizobium are improvement efforts to optimize the plant growth in critical land. The research purposes to determine the use of palm oil waste and Rhizobium on mungbean growth and yield. The experiment was conducted in post-tin mining land of Dwi Makmur Village, Bangka. The experiment used factorial randomized block design with 2 factors. The first factor was the use of palm oil waste, which consisted of Boiler Ash (L1), empty fruit bunches(L2) and decanter solid (L3). The second factor was without Rhizobium (R0) and Rhizobium application (R1). The results showed that the types of palm-oil waste application significantly affected mungbean growth and yield in post-tin minning land. Rhizobium application significantly affected growth parameters of mungbean. Mungbean growth parameters with Rhizobium application was better than without Rhizobium application. Interaction between palm oil waste and Rhizobium significantly affected seed production. Combination of empty fruit bunches with Rhizobium provided the best growth of mungbean.  Key words: mungbean, boiler ash, empty oil-palm fruit bunches, solid, post-tin mining ABSTRAK Kacang hijau merupakan tanaman legum yang potensial untuk dikembangkan pada lahan pasca tambang timah. Tanaman ini dapat ditanam di lahan kritis karena cukup toleran terhadap kekeringan. Pemberian bahan organik dan aplikasi Rhizobium menjadi salah satu upaya dalam mengoptimalkan pertumbuhan tanaman di lahan kritis. Tujuan penelitian untuk mengetahui penggunaan jenis limbah sawit dan Rhizobium terhadap respon pertumbuhan dan hasil tanaman kacang hijau. Penelitian dilaksanakan di lahan bekas tambang timah di Desa Dwi Makmur, Kecamatan Merawang, Bangka. Percobaan menggunakan Rancangan Acak Kelompok Pola Faktorial (RAKF) dengan 2 (dua) faktor. Faktor pertama adalah penggunaan limbah sawit, yang terdiri dari abu boiler (L1), TKKS (tandan kosong kelapa  sawit) (L2), dan solid (L3). Faktor kedua adalah tanpa Rhizobium (R0) dan aplikasi Rhizobium (R1). Hasil penelitian menunjukkan penggunaan jenis limbah sawit berpengaruh terhadap pertumbuhan dan hasil tanaman kacang hijau di lahan pasca tambang timah. Pemberian Rhizobium berpengaruh terhadap pertumbuhan tanaman. Pertumbuhan tanaman dengan aplikasi Rhizobium lebih baik dibandingkan dengan pertumbuhan tanaman tanpa aplikasi Rhizobium. Terdapat pengaruh interaksi antara jenis limbah sawit dan Rhizobium yang memberikan pengaruh terhadap peubah bobot 100 biji kacang hijau di lahan pasca tambang timah. Tanaman kacang hijau yang diberi bahan organik kompos TKKS dan diinokulasi dengan Rhizobium menghasilkan pertumbuhan terbaik. Kata kunci: kacang hijau, abu boiler, tandan kosong kelapa sawit, solid, pasca tambah timah
Optimalisasi Satuan Kontra Intelijen Untuk Penanggulangan Aksi Terorisme Suharyanto Suharyanto
Jurnal Ketahanan Nasional Vol 17, No 3 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkn.22698

Abstract

Rumusan alternatif model optimalisasi Satuan Kontra Intelijen Bais TNI berdasarkan identifikasi faktor internal dan eksternal yang dilanjutkan dengan analisis matrik internal-eksternal dengan metode SWOT merekomendasikan untuk memilih : (1) Strategi SO : peningkatan latihan & penguasaan teknologi dan peningkatan kinerja untuk mendukung pemberantasan terorisme (2) Strategi ST: peningkatan nasionalisme bangsa untuk menghadapi kejahatan trans nasional dan peningkatan latihan untuk menghadapi perubahan situasi politik dan keamanan nasional, (3) Strategi WO : peningkatan kerja sama komunitas intelijen serta partisipasi masyarakat, (4) Strategi WE: peningkatan kemampuan kontra intel untuk menghadapi kejahatan trans nasional dan peningkatan kemampuan dalam bidang teknologi informasi. Atas dasar rekomendasi tersebut optimalisasi Satuan Kontra Intelijen Satintel Bais TNI dengan meningkatkan kemampuan kontra intelijen melalui pendidikan, pelatihan dan penugasan serta pemenuhan matsus intelijen yang disesuaikan dengan perkembangan teknologi informasi.
MPPT BERDASARKAN ALGORITMA P&O DAN IC PADA INTERLEAVED-FLYBACK 250W Y Munandar Kolewora; Eka Firmansyah; Suharyanto Suharyanto
Telematika Vol 11, No 1: Februari (2018)
Publisher : Universitas Amikom Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (635.447 KB) | DOI: 10.35671/telematika.v11i1.603

Abstract

Interleaved-flyback adalah topologi yang lazim digunakan pada inverter mikro dalam aplikasi panel surya. Lazimnya, dalam aplikasi tersebut, diperlukan sebuah algoritma maximum power point tracking (MPPT) untuk meningkatkan perolehan energinya. Dalam tulisan ini, dua algoritma MPPT; perturb & observe (P&O) serta incremental conduction (IC) akan diterapkan pada sebuah interleaved-flyback. Dianalisis kemampuan kedua algoritma tersebut untuk menghadapi perubahan intensitas cahaya matahari dalam rentang 600-1000 W/m2. Dapat disimpulkan di akhir tulisan bahwa algoritma IC menghasilkan energi yang lebih sedikit dari P&O dengan selisih hanya 0.2%. Sehingga dapat disimpulkan bahwa kedua algoritma tersebut relatif sama dan penerapannya tergantung pada ketersediaan prosesor yang digunakan serta alasan teknis lain.Kata Kunci: MPPT, P&O, IC, Interleaved-Flyback, Panel Surya.
Internalisasi Scientific Attitude Mahasiswa Pada Praktikum Fisika Dasar I Melalui Implementasi Inductive Teaching Methods Sabar Nurohman; Suharyanto Suharyanto
Jurnal Pendidikan Matematika dan Sains No 2 (2009): Jurnal Pendidikan Matematika dan Sains Tahun XIV
Publisher : Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.35 KB) | DOI: 10.21831/jpms.v14i2.310

Abstract

Penelitian ini bertujuan untuk mengetahui bagaimana penerapan Inductive Teaching Methods pada mata kuliah praktikum Fisika Dasar I dapat meningkatkan internalisasi Scientific Attitude mahasiswa. Pendekatan yang digunakan dalam penelitian ini adalah Classroom Action Research (CAR). Melalui PTK ini, diharapkan ditemukan suatu skenario pembelajaran Inductive Teaching Methods yang paling efektif dan efisien untuk meningkatkan internalisasi scientific attitude mahasiswa. Setelah dilakukan dua siklus tindakan kelas diketahui bahwa (1) Skenario pembelajaran pada tindakan siklus II telah berhasil menginternalisasikan aspek scientific attitude mahasiswa, hal ini ditandai dengan perolehan nilai indikator scientific attitude hingga pada taraf 66%, (2) Skenario pembelajaran pada tindakan siklus II terdiri dari langkah-langkah (a) asisten mengungkapkan permasalahan eksperimen, (b) mahasiswa melakukan kajian teori, (c) mahasiswa merumuskan hipotesis, (d) mahasiswa merancang percobaan, (e) dosen melakukan uji pemahaman konsep, (f) mahasiswa melakukan percobaan, (g) Mahasiswa membuat laporan sementara, (h) dosen meminta mahasiswa untuk membuat grafik hubungan antar- variabel percobaan.Kata kunci: inductive teaching methods, scientific attitude
Kualitas Fisik, Mikrobiologis, dan Organoleptik Telur Konsumsi yang Beredar di Sekitar Kampus IPB, Darmaga, Bogor Suharyanto Suharyanto; N. B. Sulaiman; C. K. N. Zebua; I. I. Arief
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 4 No. 2 (2016): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract

This study was conducted to evaluate physical, microbiological, and organoleptic quality of layer eggs marketed around IPB campus Darmaga, Bogor. Egg samples were obtained from three market groups (supermarket, retailer, and traditional market). Each group includes three markets determined purposively, namely SM-1, SM-2, and SM-3 for supermarkets; BR-1, BR-2, and BR-3 for retailers; and TM-1, TM-2, and TM-3 for traditional markets. Physical qualities measured were egg weight, yolk index, albumen index, air sac height, pH and Aw. Microbiological quality was expressed as colony forming unit with Total Plate Count (TPC) method and the number of Escherichia coli colony formed. Organoleptic properties evaluated were hedonic and hedonic ranking test. All physical and microbiological qualities of eggs showed no significant differences among markets origin. This study confirmed that eggs excluded from SNI 3926-2008 (Indonesia National Standard for consumption egg) and other scientific findings. However, the low physical and microbiological qualities did not cause any refusing on the acceptability of sensorial properties by panelists.
TINGKAT PARTISIPASI ANGGOTA TERHADAP KOPERASI UNIT DESA (KUD) MULIA DI DESA SARI MAKMUR KECAMATAN PANGKALAN LESUNG KABUPATEN PELALAWAN Suharyanto Suharyanto; Rosnita Rosnita; Cepriadi Cepriadi
Jurnal Online Mahasiswa (JOM) Bidang Pertanian Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Pertanian

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Abstract

The purpose of this research was to know the degree participant of member Mulia Coorporation in vilage Sari Makmur, Pangkalan Lesung in palelawan regency. This research used survey method.This research was using random sampling. The total sample thus research was 41 people. The sample found randomly from 500  mamber Mulia Coorporation. The colecting data of this research was using primary data and secondary data. To analize the degree and problems at participant of this research was using descritive method. To obtain the degree of participantion of getting result at meeting, implementation of participation in cooperation activities, participant to gain the function of service, and evalution of programs cooperation. The degree of members participation measured by using quisioner of likert scale the degree of members participant of members Mulia Cooperation in Sari Makmur , Pangkalan lesung, Pelalawan Regency was high with score 3,60  high of the degree participant on member Mulia Cooperation support by high partipant members on meeting, implemantion, service, also evolution program of cooperation. In another side the programs Mulia Cooperation in Sari makmur, Pangkalan Lesung, Pelalawan regencyhad some problems that related by the  degree of participant were pasif on cooperation activities, attedenc, lack gave ideas on meeting, less armada dum truck, and participant on activiy and evolution of programs Mulia Cooperation.Keywords : The Degree of participant, peformace of Member, Cooperation, KUD Mulia
PEMANENAN AIR HUJAN SEBAGAI PEMENUHAN KEBUTUHAN AIR BERSIH PENGUNGSI BENCANA BANJIR Abdul Roviq; Hartuti Purnaweni; Suharyanto Suharyanto
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 1 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Air bersih dan sanitasi merupakan kebutuhan penting pada saat terjadi bencana banjir, namun banjir menyebabkan kesulitan pemenuhan air bersih bagi pengungsi sehingga  mengakibatkan munculnya berbagai macam penyakit seperti diare, muntaber  ataupun penyakit kulit dan gatal-gatal, sehingga diperlukan konsep penanganan air bersih pada bencana banjir. Penelitian ini dilakukan di daerah rawan banjir di Kelurahan Wonosari Kecamatan Ngaliyan Kota Semarang. Tujuan penelitian yaitu melakukan identifikasi  tingkat manfaat penggunaan sumber air bersih  alternatif bagi pengungsi banjir dengan sasaran penelitian mendapatkan informasi  tentang pengadaan sumber air bersih  alternatif oleh pengungsi banjir  Kelurahan Wonosari yaitu dari sumber alami non perusahaan daerah air minum (non PDAM). Pemanfaatan sumber alami non PDAM  dilakukan  karena keterbatasan kemampuan Pemerintah Kota Semarang  dalam menyediakan akses kebutuhan air bersih  melalui PDAM. Sumber air bersih pengungsi korban banjir di Kelurahan Wonosari dapat diperoleh dari mengelola air melalui Pemanenan Air Hujan (PAH) secara individu. Alternatif lain dengan sistem  pengolahan   mobile  yang dapat melayani kelompok lebih besar. Alternatif  ini  diusulkan melalui Badan Penanggulangan Bencana Daerah (BPBD) Kota Semarang sebagai dasar penempatan tenda pengungsi untuk kesiapsiagaan terhadap banjir yang mungkin terjadi lagi.  Kata Kunci: Sumber Air Alternatif,  Banjir, Pengungsi, Pemanenan Air Hujan
DEBRIS FLOW DISASTER MITIGATION THROUGH COMMUNITY-BASED INTEGRATED SEDIMENT MANAGEMENT (BEST PRACTICE IN MT. MERAPI AREA, INDONESIA) Kazuhiko Otani; Suharyanto Suharyanto
Journal of the Civil Engineering Forum Volume: XXI/3 - September 2012
Publisher : Department of Civil and Environmental Engineering, Faculty of Engineering, UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (758.077 KB) | DOI: 10.22146/jcef.18921

Abstract

Mt. Merapi is one of many active volcanoes in Indonesia which erupts frequently. The small eruption occurred nearly every year, whereas the big ones occurred at approximately once every five years. The eruption often produces impacts at both positive and negative view points, such as production of sediment as construction material and damage on infrastructures due to debris flow occurrences respectively. The eruption produces two types of disasters, i.e. primary disaster (such as ash fall, pyroclastic flow, and lava flow), and secondary disaster such as debris flow. This paper presents the long term effort on the development of community participation in the sand mining management as one of strategic disaster mitigation activities. The raising awareness of the community on the necessity of conducting proper sand mining management and its effect on reducing the risk due to debris flow disaster has shown the effectives of the approach being introduced. The local government acceptance on the presence of the community participation in the whole system of sediment management may involve further collaboration between the local government authority and community society in the future.
INDUKSI KALUS DENGAN 2,4D DAN BAP PADA EKSPLAN DAUN VEGETATIF DAN GENERATIF TEMPUYUNG (Sonchus arvensis L.) Shilfiana - Rahayu; Suharyanto Suharyanto
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3677

Abstract

Tempuyung (Sonchus arvensis L.) is a plant that has many benefits and medicinal potencial. This plant is contain high flavonoids and terpenoids. The propagation of this plant is very important to do to provide medicinal ingridients on a prefab scale and to produce materials that have a high compound content. The tissue culture is one of the fastest ways in plant propagation and can increase the content of secondary metabolites in plants. The method to produce high compounds and produce somatic embryos in large quantities can be through callus culture. The research aim was to find the effect of 2,4D and BAP hormones and leaf explant variations on the growth tempuyung callus. In this research, a combination of 2,4D (0; 0.5; 1 ppm) and BAP (0; 0.5; 1 ppm) hormone was used in inducing callus, and explants used in this research were vegetative leaves and generative leaves of tempuyung. The results of this study stated that callus was produced within 10-14 days, with the friable callus texture and yellow in color. Vegetative leaf explants initiate callus faster than generative leaves. Other results showed that the best combination in callus production was BAP 0.5 ppm and 2.4D 1 ppm, and the most optimal leaf explants in producing callus were found in vegetative leaf explants