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Development of Domestic Cat Embryo Produced by Preserved Sperms KARTINI ERIANI; ARIEF BOEDIONO; ITA DJUWITA; SONY HERU SUMARSONO; AL-AZHAR AL-AZHAR
HAYATI Journal of Biosciences Vol. 15 No. 4 (2008): December 2008
Publisher : Bogor Agricultural University, Indonesia

The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 oC, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 oC for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (P < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 oC in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos. Key words: gamet, preservation, in vitro fertilization

Low Concentration of Ethylene Glycol Improved Recovery Rate of Human Spermatozoa After Vitrification (ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKAN RECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) Rini Widyastuti; Sony Heru Sumarsono; Arief Boediono; Siti Darodjah Rasad
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The use of cryoprotectants for the cryopreservation of human spermatozoa, oocytes, zygote, earlycleavage stage of embryos and blastocyst is an integral part of almost every human In Vitro Fertilizationprogram. Moreover, the cryopreservation of these types of cells by direct plunging into liquid nitrogen (-196°C) usually requires a high concentration of cryoprotectant with a consequent of cytotoxic effect. Theaim of this study was to observe the effect of ethylene glycol concentration on the spermatozoa recoveryrate following vitrification process. Earle’s balanced salt solution + 0.25 M sukrosa + 1 % human albumineserum as basic solution supplemented with some different concentrations of etylene glycol (ie: 36.25%;18.25%; 9.12%; 4.56%; 1.14% and 0.57%) were used to evaluate the motility and viability of spermatozoafollowing vitrification. Human’s spermatozoa from ejaculates with progressive motility and viability above50% were used as samples. Samples were mixed with vitrification solution and then loaded into 0.25 mLstraws, equilibrated for 10 minutes at room temperature before plunged into liquid nitrogen. Spermatozoathawing was done at 24 hours after the vitrification. The results showed that, the decrease of spermatozoamotility and viability were observed at the highest (100%, 96.70%, respectively) in the samples that wereadded with vitrification medium contained 36.25% of ethylene glycol. On the other hand, the decrease ofthe spermatozoa motility and viability were found at the lowest (14.11%, 43.81 %, respectively) in thesamples without ethylene glycol supplementation. It can be concluded that the highest spermatozoa recoveryrate was obtained from the vitrification using a low concentration of ethylene glycol.

Ekstrak Etanol Biji Pinang Muda (Areca catechu L) Meningkatkan Apoptosis pada Sel Testikular Mencit (Mus musculus L) Sony Heru Sumarsono; Eka Pasana Pujowati Indra Wibowo Ayda T Yusuf
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 3, No 1 (2018): February 2018
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v3i1.1890

Biji pinang memiliki potensi sebagai bahan antifertilitas dan diduga dapat menginduksi apoptosis sel-sel spermatogenik. Penelitian ini membahas tentang perubahan jaringan testikular mencit akibat pemberian ekstrak etanol biji pinang muda. Ekstrak etanol biji pinang muda disiapkan dengan maserasi, penjemuran, perendaman dan evaporasi hingga diperoleh serbuk ekstrak etanol yang kemudian dilarutkan dalam larutan Gum Arab 1% sesuai dosis. Mencit jantan galur Swiss Webster (umur8 minggu, berat 30-35 gram) dibagi dalam 5 kelompok perlakuan: (1) K = Kontrol, (2) KP = Kontrol pelarut (Gum Arab 1%), (3) P1 = Perlakukan 1 (dosis 300 mg/kg berat badan (bb)), (4) P2= Perlakuan 2 (dosis 500 mg/kg bb), dan (5) P3=Perlakuan 3 (dosis 700 mg/kg bb), diberikan secara oral (gavage) selama 17 hari. Mencit ditimbang sebelum perlakuan dan setelah perlakuan. Mencit dibunuh pada hari ke 18 dengan dislokasi leher, testis diisolasi, ditimbang dan difoto untuk mengetahui penambahan berat dan ukuran. Preparat histologi sayatan testis disiapkan dengan pewarnaan HE dan TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay untuk mengamati apoptosis. Hasil pengamatan menunjukkan terjadi (1) penurunan berat relatif testis kelompok P3, (2) ukuran testis relatif lebih kecil pada kelompok perlakuan, (3) perbedaan struktur jaringan testis pada kelompok P2 dan P3, al: (a) jumlah tubulus seminiferus lebih sedikit, (b) diameter tubulus seminiferus lebih kecil, (c) jarak antar tubulus seminiferus lebih renggang, (d) penurunan jumlah sel testikular, dan (e) peningkatan jumlah sel yang mengalami apoptosis. Ekstrak etanol biji pinang menyebabkan peningkatan apoptosis dan memiliki potensi sebagai agen alami antifertilitas pria.

Identification of single nucleotide polymorphisms on the D-loop region of mtDNA in Sundanese population Wolly Candramila; Sony Heru Sumarsono; Bambang Suryobroto; Maelita Ramdani Moeis
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (884.282 KB)

Identification of sequence polymorphism on the D-loop region of mtDNA has been done for various purposes, including health and medical treatment. In this research, single nucleotide polymorphisms were identified in the D-loop region of mtDNA of the Sundanese population in western Java. A total of 118 unrelated and healthy Sundanese probands were collected from closed-traditional kampung adat and open communities distributed in 14 cities and regencies in western Java. DNA amplification and direct sequencing of the D-loop region were proceeded using primers L15990 and H409. Multi-alignment was conducted not only intrapopulation but also with D-loop sequence data stored in GenBank for comparison. In this research, we categorized high-frequency SNPs as less effective for identification in population studies because of their presence in other populations outside Indonesia. Meanwhile, lower-frequency SNPs showed typical variants of Sundanese haplotypes. On the other hand, rare or low-frequency SNPs should be re-examined in larger size of samples to have a better understanding of risk factors for many diseases.

Identification of single nucleotide polymorphisms on the D-loop region of mtDNA in Sundanese population Wolly Candramila; Sony Heru Sumarsono; Bambang Suryobroto; Maelita Ramdani Moeis
Tropical Genetics Vol. 1 No. 1 (2021)
Publisher : Genetikawan Muda Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Identification of sequence polymorphism on the D-loop region of mtDNA has been done for various purposes, including health and medical treatment. In this research, single nucleotide polymorphisms were identified in the D-loop region of mtDNA of the Sundanese population in western Java. A total of 118 unrelated and healthy Sundanese probands were collected from closed-traditional kampung adat and open communities distributed in 14 cities and regencies in western Java. DNA amplification and direct sequencing of the D-loop region were proceeded using primers L15990 and H409. Multi-alignment was conducted not only intrapopulation but also with D-loop sequence data stored in GenBank for comparison. In this research, we categorized high-frequency SNPs as less effective for identification in population studies because of their presence in other populations outside Indonesia. Meanwhile, lower-frequency SNPs showed typical variants of Sundanese haplotypes. On the other hand, rare or low-frequency SNPs should be re-examined in larger size of samples to have a better understanding of risk factors for many diseases.

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