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Effect of Zn Supplemented to Immune Status Premenopausal Women Intervented with Isoflavoned Drinking HERY WINARSI; DEDDY MUCHTADI; FRANSISKA RUNGKAT ZAKARIA; AGUS PURWANTO
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

The research was conducted to find out the effect of Zn supplement to immune status of premenopausal women intervented with isoflavoned drinking. Respondents were 22 women, more than 40 year of age. They were divided into two groups, i.e. 11 women intervented with isoflavone, and other 11 women intervented with isoflavone and 8 mg Zn. The activities of SOD, catalase and GPX were determined by spectrophotometer, thymulin levels by ELISA, whereas Zn levels by AAS. Result showed that Zn had significantly increased SOD lymphocyte activities (p=0.002) and thymulin plasma (p=0.011). Zn had increased catalase (p=0.103) and GPX (p=0.322) as well, but Zn plasma had decreased (0.163). It was indicated that Zn had improved the immune status by increasing lymphocyte and thymus cells activities.

SENYAWA BIOAKTIF RIMPANG JAHE (Zingiber officinale Roscue ) MENINGKATKAN RESPON SITOLITIK SEL NK TERHADAP SEL KANKER DARAH K-562 IN VITRO [Ginger Root Bioactive Compounds Increased Cytolitic Response of Natural Killer (NK) Cells Against Leucemic Cell L Tejasari .; Fransiska Rungkat Zakaria
Jurnal Teknologi dan Industri Pangan Vol. 17 No. 2 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Natural killer (NK) cell, a kind of lymphocyte cells, plays an important role in attacking infectious, immature, and cancer cell. Its function could be modulated by food bioactive compounds. This experiment was conducted to investigate the effects of ginger root bioactive compounds such as oleoresin, gingerol, and shogaol on cytolitic response of NK cell in vitro. Lymphocyte cells were isolated by centrifugation on ficoll-hypaque density (1,77 ?0,001 g/ml) method. Leukemic cells line K-562 as target cells(TC) labelled by [3H]-timidin, together with lymphocyte as effector cell (EC) were cultured in two ratio levels of EC : TC equal to 1:50 and 1:100), and two culture conditions, for 4 hours, respectively. Paraquate dichloride (1,1-dimethyl-4,4-bipyridilium dichloride) 3 mM was used to induce stress oxidative circumstance. Cytolytic capacity of NK cells was determined by percentage of TC lysed by NK cells, in normal and oxidative stress conditions. Statistical analysis showed that the effects of ginger bioactive compounds on cytolytic response of NK cell depended on the culture conditions, as shown by cultures in the presence of oleoresin, and gingerol, but not shogaol. In the lymphocyte culture without stress oxidative, oleoresin, gingerol and shogaol compounds increased significantly cytolytic response of NK cells cultured at a ratio of TC : EC equal to 1:50, with the highest increament of 65 % at oleoresin concentration of 50 ?g/ml. However, in culture at a ratio of TC : EC equals to 1:100, only oleoresin at a concentration of 50 ?g/ml increased significantly cytolytic response of NK cells with the highest increament of 8 %. Shogaol did not affect significantly NK cells cytolytic response. Under stress oxidative conditions, shogaol increased significantly cytolytic response of NK cells cultured at a ratio of TC:EC equal to 1:50, but the highest increament of 56 % , was by oleoresin at concentration of 50 ?g/ml. Meanwhile, oleoresin and gingerol did not increased significantly cytolytic response of NK cells. At a culture- ratio of TC: EC equal to 1:100, gingerol increased significantly cytolytic response of NK cells of 21 % at a concentration of 50 ?g/ml. However, oleoresin did not increased significantly cytolytic response of NK cells. In contrast, shogaol decreased significantly cytolytic response of NK cell with the highest decreament of 16 % at 200 ?g/ml. These findings verified that ginger root bioactive compounds increased cytolityc response of NK cells in destroying cancer cells, at certain conditions and concentrations, especially at low concentrations. Key words : ginger bioactive compound, oleoresin, gingerol, shogaol, stress oxidative, effector cell, target cell, natural killer cell, cytolytic response

In Vitro Antioxidant Activity of Stabilized Rice Bran and Its Fraction Evy Damayanti; Fransiska Rungkat Zakaria; Hidayat Syarief; C Hanny Wijaya; Djoko Said Damardjati
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 1 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Some Researches indicated that oryzanol had antioxidant activity, however, the information about the oryzanol role in the prevention of low density lipoprotein (LDL) and human lymphocyte from oxidation under oxidative stress was still limited. The objective of this study was the investigate the antioxidant activity of oryzanol at concentrations based on rice bran beverage model in preventing LCL and lymphocyte from oxidation. Human plasma were supplemented with the samples of : rice bran oil (RBO), unsaponifiable matter and oryzanol IR-64, oryzanol IR-64 3x and oryzanol standard at the concentrations of 308.3, 22.2, 5.2, 10.4, and 10.4 Ã‚Âµg/ml, respectively. Afterward, the human LDL were collected by ultracentrifuge and diluted until a concentration of 200 Ã‚Âµg protein/ml was reached. Human LDL isolates were then oxidized with CuSO4 5 Ã‚ÂµM for measuring antioxidant activity of the sample. The length of incubation, H2O2 concentration, period of sample supplemented into human lymphocyte culture were determined before the antioxidant activity of RBO and its fraction in lymphocyte was measured. The samples used in the lymphocyte were RBO IR-64, unsaponifiable matter IR-64, and oryzanol standard at the concentrations of 133.2 – 2, 132.0 Ã‚Âµg/ml, 9.6 – 153.6 Ã‚Âµg/ml, and 2.4 – 37.7 Ã‚Âµg/ml, consecutively. The result showed that malonaldehyde concentration in human LDL decreased significantly (α = 0.05), 15 – 41% and 39 – 56% compared to the control. The absorbance of living lymphocyte cell in culture was not influenced by the type and concentration of RBO and its fraction. The addition of hydrogen peroxide (H2O2) 3 mM into culture sifnificantly lowered the absorbance as compared to culture without (H2O2). Key words :Oryzanol, oxidative stress, LDL-oxidized, lymphocyte and antioxidant activity.

PENGARUH KONSUMSI MINUMAN BEROKSIGEN TERHADAP INFLAMASI DAN KAPASITAS ANTIOKSIDAN PENDERITA PENYAKIT-PARU-OBSTRUKTIF-KRONIK (PPOK) [Influence of Oxygenated Water Consumption in Chronic-Obstructive-Pulmonary-Disease (COPD) Patients] Fransiska Rungkat Zakaria; Intan Nurul Azni; Elvira Syamsir; Amalia KM.; Cholid Yamani
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

PENGARUH KONSUMSI MINUMAN BEROKSIGEN TERHADAP INFLAMASI DAN KAPASITAS ANTIOKSIDAN PENDERITA PENYAKIT-PARU-OBSTRUKTIF-KRONIK (PPOK) [Influence of Oxygenated Water Consumption in Chronic-Obstructive-Pulmonary-Disease (COPD) Patients]Fransiska Rungkat Zakaria1), Intan Nurul Azni2)*, Elvira Syamsir2), Amalia KM.3) dan Cholid Yamani3)1)Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor2)Program Studi Ilmu Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor3) Klinik dr. Katili, Jl. Raya Dramaga, Bogor Diterima 01 Agustus 2013 / Disetujui 05 Februari 2014ABSTRACT Chronic Obstructive Pulmonary Disease (COPD) is one of the leading cause of death in the world that represents an important public health problem. Oxygenated water is water added with high concentration of oxygen such that the oxygen concentration is higher than normal water. The objective of this study was to assess the influence of oxygenated water consumption on the alteration of proinflammatory cytokines (TNF-α, IL1-β, and IL6) and antioxidant capacity of COPD patients. Sixteen COPD patients were allowed to drink 385 mL oxygenated water two times a day for 21 days. The alteration of proinflammatory cytokines and antioxidant capacity are measured by comparing plasma concentration before and after intervention. The results suggest that oxygenated water consumption significantly reduce proinflammatory cytokines plasma (TNF-α, IL1-β, and IL6) at 5% significance level with 81.25% of respondents having lower TNF-α, 75% of respondents with lower IL-1β, and 62.25% of respondents having lower the IL-6 in plasma concentration after 21 days intervention. There were 43.75% of respondents with decreased antioxidant capacity concentration. However, it was not significant at the 5% level significance. Decrease in antioxidant capacity was probably a resulted from poordiet and drugs consumption during the intervention period.

PENGARUH MINUMAN BEROKSIGEN TERHADAP SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN [Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance] Cesar Welya Refdi; Fransiska Rungkat Zakaria; Puspo Edi Giriwono
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

PENGARUH MINUMAN BEROKSIGEN TERHADAP SISTEM IMUN, KADAR MALONALDEHIDA DAN PERFORMA RESPONDEN MAHASISWA OLAHRAGAWAN[Effect of Oxygenated Water on Immune System, Malonaldehyde Content and Student-Athlete Performance]Cesar Welya Refdi, Fransiska Rungkat Zakaria* dan Puspo Edi GiriwonoDepartemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor Diterima 23 Agustus 2013 / Disetujui 01 Juli 2014ABSTRACT Exercise requires more oxygen input and cannot be fully obtained through breathing. The body has a homeostatic system, when more ATP is needed, the more oxygen is required by the body. Limited supply of oxygen such as those happens in athlete’s body automatically switches the respiration from aerobic into anaerobic. This situation results in building up of lactic acid which in turn lowers the energy for cellular metabolism as well asenergy for exercise. The purpose of this study was to assess the effects of short-term and long-term drinking of oxygenated water on lactic acid and energy expenditure (EE) in exercise performance; Cluster of Differentiation-4 (CD4), CD8, CD56, Interleukin-6 (IL-6) contents; and level of malonaldehyde. During short-term intervention, 12 male student volunteers drank oxygenated (50, 80 and 130 ppm) water and normal mineral water, 15 minutes before treadmill and after treadmill (10 Kmh). The lactic acid concentration was measured before and after treadmill, and 5 minutes after the reconsumption of the oxygenated water. During long-term intervention, 17 male student volunteers drank oxygenated (100 ppm) water twice a day for 21 days. The blood plasma and lymphocyte sampled before and after intervention were analyzed for the same parameters. The results showed that oxygenated water had no effect on lactic acid accumulations and EE. Similarlyt he long-term study showed that oxygenated water did not affect CD4 and CD8, had no harmful effect on IL-6 and malonaldehyde, yet it significantly increased the CD56 content.

Konsumsi Tahu Kedelai Hitam untuk Memperbaiki Nilai SGOT/ SGPT dan Aktivitas Antioksidan Plasma Penderita Diabetes Tipe 2 Fransiska Rungkat Zakaria; Delina Puspa Rosana Firdaus; Nancy Dewi Yuliana
JURNAL PANGAN Vol. 25 No. 2 (2016): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v25i2.325

Type 2 diabetes mellitus pathogenesis is characterized by high blood glucose which can be triggered by several factors such as oxidative stress, unhealthy diet, and poor lifestyle. The high content of bioactive components and low content of digestible carbohydrate in black soybeans tofu might contribute to the body antioxidant supply and control blood glucose levels. This study aimed to evaluate the effects of black soy tofu consumption toward plasma SGOT/SGPT and antioxidant capacity. The intervention of black soybean tofu was conducted for 28 days in 15 type-2 diabetic respondents. The results showed that black soybean tofu significantly (p ˂ 0.05) increased plasma antioxidant activity level of respondents, from 45.79 ± 3.31 percent to 53.05 ± 4.44 percent. SGOT / SGPT tests showed a decrement ((p ≥ 0.05), from 14.27 ± 3.81 / 21.07 ± 6.73 (U/L) to 12.73 ± 2.34 / 18.60 ± 4.29 (U/L). The processing of black soybeans into tofu caused a decrease in antioxidant activity, from 144.06 mg AEAC to 45.27 mg AEAC and the total anthocyanin, from 12.27 mg to 1.805 mg/100 g. The results suggested that black soybean tofu improved the health profile of the diabetic respondents by increasing plasma antioxidant capacity and decreasing plasma SGOT/SGPT level.

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