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All Journal HAYATI Journal of Biosciences JOURNAL OF COASTAL DEVELOPMENT
JOEDORO SOEDARSONO
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education

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Molecular Detection of Endophytic Bacteria on Plantlet Tissue of Sugarcane WIWIK EKO WIDAYATI; JOKO WIDADA; JOEDORO SOEDARSONO
HAYATI Journal of Biosciences Vol. 14 No. 4 (2007): December 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.154 KB) | DOI: 10.4308/hjb.14.4.145

Abstract

Endophytic bacteria live in plant host tissues without causing any symptoms. The aim of this study was to examine the indigenous endophytic bacteria on sugarcane plantlets produced from the young leaf cells by using tissue culture techniques. To detect the existence of endophytic bacteria in the plantlet tissue, a series of molecular method based on PCR were applied by using ribosomal intergenic spacer (RIS) primer followed by 16S rDNA partial sequence and single strand conformation polymorphism (SCCP). The results showed that the molecular method could detect the existence of bacteria in the tissues. Using the same methods, the bacteria were also found in other developmental stages of sugarcane (explants, differentiated tissues and callus). Key words: endophytic bacteria, PCR, RIS, 16S rDNA, SSCP
CHARACTERIZATION AND IDENTIFICATION OF STRAIN KM221, A NOVEL MCPA HERBICIDE-DEGRADING BACTERIUM ISOLATED FROM CORAL SURFACE, MENJANGAN KECIL ISLAND, KARIMUNJAWA Agus Sabdono; Ocky Karna Radjasa; Joedoro Soedarsono
JOURNAL OF COASTAL DEVELOPMENT Vol 6, No 3 (2003): Volume 6, Number 3, Year 2003
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

In this study, bacterial strain KM221 was isolated from coral   tissue in Menjangan Kecil Island, Karimunjawa, Indonesia. This strain is  facultative anaerobic  with MCPA (2-methyl-4-chlorophenoxy acetic acid) serving as the only known energy sources.  Microscopy  of isolate revealed that strain KM221 is gram-positive, catalase-positive,  rod, spore-forming bacterium, motile,  opaque, hair-like outgrowth and unpigmented colonies. The bacterium could not be identified on the basis of its carbon-source-utilization pattern, but a partial sequencing of the 16S rDNA analysis suggest that this strain is closely related to Bacillus iodinum.The ability to degrade MCPA herbicide was examined qualitatively in EMBA indicator medium.  This bacterium grew exponentially with MCPA as  the sole source of energy and carbon. The maximum growth rate (m max)  and the  saturated concentration on MCPA (Cs) were determined to be  0.8024 h-1 and  5.10 mg/l MCPA, respectively.
SPECIES DETERMINATION OF GREEN ALGAE ISOLATED FROM JEPARA COASTAL REGION BASED ON MICROBIOLOGICAL, ECOPHYSIOLOGICAL AND MOLECULAR CHARACTERIZATION FOR IMPROVEMENT OF CAROTENOID PRODUCTION Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini; Triwibowo Yuwono; Joedoro Soedarsono
JOURNAL OF COASTAL DEVELOPMENT Vol 10, No 1 (2006): Volume 10, Number 1, Year 2006
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

A local isolate of green algae called C1 from Jepara waters is usually used as a source for carotenoid supplement for animal fisheries in the local area. This indigenous algae has been successfully purificated.  Although the local isolate was known as eucaryotic green algae Dunaliella, our previous molecular study by 18S rDNA analysis to determine the species of this organisms showed negative result. In order to improve carotenoid production especially detection of biosynthetic pathway from the organisms investigated in this study, the main purpose of this study was species determination of local isolate of green algae based on microbiologycal, ecophysiologycal and molecular characterization. The results of this research  indicated that local isolate of green algae posseses Cyanobacteria characteristic, especially Synechocystis. Analyses with 16S rDNA sequence from genome of green algae isolates were also in accordance with these results, showing close similarities with Synechocystis 16S rDNA sequence.  However, it should be noted that, instead of having Synechocystis dominant feature, it was also found that local isolate of green algae exhibit different characteristic in having chlorophyll a, chlorophyll b and lack of phycobillins. This character was typical for abberant Cyanophyta, Prochlorophyta.
Co-Authors Agus Sabdono Endang Kusdiyantini Hermin Pancasakti Kusumaningrum JOKO WIDADA Ocky Karna Radjasa Triwibowo Yuwono WIWIK EKO WIDAYATI