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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Manfish Journal

Yuni Puji Hastuti

Department Of Aquaculture, Faculty Of Fisheries And Marine Science, IPB University

Author-ID : 2421151

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Library & Information Science

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Characteristics and Activity of Anti Qourum Sensing Bacillus spp. Isolated from Penaeus vannamei Shrimp Ponds Iman Rusmana; Yuni Puji Hastuti; Rizal Khoirun Alfisah; Alina Akhdiya
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.97-106

Certain strains of V. parahaemolyticus carry a gene that encodes a toxin that causes Acute hepatopancreatic necrosis disease (AHPND) in P. vannamei. AHPND attacks shrimp post larvae within 20-30 days after stocking causing up to 100% mortality. The expression of these virulent genes is controlled by the quorum sensing system. This system is inhibited by an anti-quorum sensing (AQS) mechanism. Several Bacillus strains have AQS mechanism by producing AHL-Lactonase enzyme. Therefore, this study aimed to obtain Bacillus spp. having AQS activity for controlling AHPND. The study was conducted from isolation and selection of Bacillus isolates, as well as determination of AQS activity. From 22 samples consisting of shrimp intestines, water and pond sediment samples, a total of 151 isolates of Bacillus spp. were isolated. The screening test for AQS activity obtained 11 isolates that showed AQS activity on Cromobacterium violaceum. Determination of violacein pigment in liquid cultures of C. violaceum showed the index value of the pigment formation was between 0.025-0.166 and 0.026-0.567 at 24-hour and between 48-hour incubations, respectively. The quantitative analysis of violacein production showed that there were six isolates of Bacillus could inhibit the pigment production more than 75%. The isolates were identified as Bacillus cereus (four isolates), Bacillus thuringiensis (one isolate), and Bacillus velezensis (one isolate), respectively. The molecular analysis had confirmed that the isolates have aiiA genes encoding AHL-lactonase enzyme. These Bacillus isolates have potential application for controlling AHNPD disease.

The Effect of Cirata Reservoir Sediment on Early Developmental Stage of Common Carp (Cyprinus carpio) Embryo Yuni Pujihastuti; K. Nirmala; I. Effendi
Jurnal Akuakultur Indonesia Vol. 8 No. 2 (2009): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Sedimentation at Cirata reservoir may directly and indirectly influence fish particularly fish which have an adhesive characteristic at its early developmental stage such as common carp (Cyprinus carpio). Sample of sediment was collected from Cirata reservoir using Eikmand dredge at a depth of 80 m. The sample was subsequently centrifuged at 5500 rpm for 10 min. The supernatant obtained was then used for toxicity test on common carp at early developmental stage. In this test, four treatments were applied based on the concentration of sediment supernatant, namely: 0, 8.33, 16.60 and 24.90 %. The results showed that a higher sediment supernatant concentration resulted in lower egg yolk absorption rate, lower relative growth rate in length, lower egg yolk efficiency and higher egg and larval abnormality. Higher sediment supernatant concentration also resulted in lower hatching percentage of common carp larva. The damage of eggs and larval morphologies in treatments with sediment supernatant was likely caused by the presence Pb and organic matters which act in synergy. Keywords : sediment, Cirata, embryo, common carp ABSTRAK Sedimentasi di Waduk Cirata secara langsung dan tidak langsung akan berpengaruh terhadap kehidupan ikan khususnya tahap awal perkembangan ikan yang bersifat adhesiveseperti ikan mas (Cyprinus carpio). Sampel sedimen waduk Cirata diambil dengan Eikmand dredge pada kedalaman 80 m. Hasil ekstrak di sentrifugasi dengan kecepatan 5500 rpm selama 10 menit untuk diambil air pori sedimennya. Air pori digunakan sebagai bahan uji toksisitas terhadap perkembangan awal ikan mas dengan perlakuan 0; 8,33; 16,60 dan 24,90 %. Hasil uji toksisitas diperoleh bahwa semakin tinggi konsentrasi air pori dari sediment maka semakin rendah laju penyerapan kuning telur Laju pertumbuhan relatif panjang embrio pada berbagai konsentrasi juga diperoleh bahwa semakin tinggi konsentrasi air sedimen maka semakin rendah laju pertumbuhan relatif panjang embrio Efesiensi pemanfaatan kuning telur menunjukkan bahwa semakin tinggi konsentrasi air pori sedimen maka semakin rendah efesiensi kuning telurnya. Semakin tinggi konsentrasi air pori sediment maka semakin tinggi pula abnormalitas telur dan larva ikan mas. Rata-rata derajat penetasan telur menunjukkan bahwa semakin tinggi konsentrasi air pori maka semakin rendah derajat penetasan telurnya. Berdasarkan kerusakan morfologi telur dan larva pada perlakuan diduga yang berpengaruh adalah timbal dan bahan organik yang bekerja secara sinergis. Kata kunci : sedimen, Cirata, embrio, ikan mas

Nitrification and denitrification in pond Yuni Puji Pujihastuti
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

More of waste than pond aquacultutre system produced, will increase sedimentation in the bottom. Ammonium and nitrite compounds are other forms of inorganic nitrogen in the pond. Nitrogen anorganic consist of ammonia, ammonium, nitrit, nitrat and nitrogen. Degradation of process metabolic biota culture waste can biologically be nitrat compound one of the forms that are not toxic in the nitrification process. Five process of nitrogen biogeochemical cycle in the container cultivation is the amonification, nitrification, nitrogen assimilation, denitrification and nitrogen fixation. Nitrogen is the one of the compound in the overlay/ top stratification sediment. Improvement of speed degradation will be success if the pond bottom on aerobic condition. Survival rate of tiger shrimp in the laboratory scale can be increase by administration of nitrification and denitrification bacteria should not just in the water kolom of pond engineering but also at the bottom pond layer at the preparation step. Depht of the sediment 15 cm in day zero, intensive pond have been produced of nitrit and ammonium with the producing bacteria. Application of nitrification and denitrification bacteria in the sediment and water coloum can be performed as the measurenment and evaluation nitrit, nitrat and ammonium abudance. Key words: ponds, nitrogen inorganic, nitrification, denitrification ABSTRAK Semakin banyak limbah kegiatan yang dihasilkan dalam sistem budidaya tambak, akan meningkatkan sedimentasi dalam dasar tambak. Senyawa amonium dan nitrit merupakan bentuk lain dari nitrogen anorganik dalam tambak. Nitrogen anorganik terdiri terdiri dari amonia (NH3-), amonium (NH4+), nitrit (NO2-), dan nitrogen (N2). Secara biologis, proses perombakan sisa metabolisme biota budidaya dapat menjadi nitrat (NO3), suatu bentuk yang tidak berbahaya dalam proses nitrifikasi. Lima proses siklus biogeokimia nitrogen yang terjadi di wadah budidaya adalah amonifikasi, nitrifikasi, asimilasi nitrogen, denitrifikasi, dan fiksasi nitrogen. Nitrogen merupakan senyawa yang biasanya terletak di lapisan paling atas dalam sedimen. Peningkatan kecepatan degradasi akan dapat dicapai jika sedimen berada dalam kondisi aerobik. Tingkat kelangsungan hidup udang pada uji skala laboratorium memperlihatkan bahwa penambahan bakteri nitrifikasi dan denitrifikasi terseleksi dapat meningkatkan kelangsungan hidup udang windu Aplikasi pemberian bakteri nitrifikasi dan denitrifikasi tidak seharusnya hanya dalam air saja, namun juga dalam pengolahan tanah dasar tambak. Pada kedalaman sedimen 15 cm hari ke-0, tambak intensif telah terdapat bakteri penghasil nitrit dan amonium dan kelimpahannya semakin meningkat seiring dengan bertambahnya umur udang. Aplikasi pemberian bakteri nitrifikasi dan denitrifikasi di sedimen dan kolom perairan dapat dilakukan seiring dengan pengukuran dan evaluasi kondisi nitrit, nitrat dan ammoniumnya. Secara alami, dalam kolom perairan telah terdapat senyawa nitrit, nitrat dan amonium, seberapapun itu perlu diimbangi dengan kebijakan dalam pemberian bakteri dari luar. Kata kunci: tambak, nitrogen anorganik, nitrifikasi, denitrifikasi

Profiles of traditional farms: soil texture, total inorganic N and bacteria-producing estate Yuni Puji Hastuti; Iman Rusmana; T. Widiyanto
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Pond traditional system is the pond in still activity with a symple management system. This activity indicated by low technology and relatively low production level. Aquaculture activities in traditional pond not loss from nitrification and denitrification prosess, however this process is more low production rather than semiintensive and intensive system. This study aims to observe abundance of bacteria nitrification along with changes soil texture, and N-organic in the soil of traditional pond. Chemical and biological analyses were done using spectroscopy and Most Probable Number methods to determine the amount of nitrite and ammonium production of bacteria. Based of the result, each stratum traditional ponds have relatively similar abundance in nitrite producing bacteria of 7.08-7.47 Log CFU/g. Increasing abundance in ammonium producing bacteria was found in all stratum, range from 5.63 Log cfu/g to 8.12 Log cfu/g. From the first day of preparation, traditional ponds have a lot of nitrite and ammonium producing bacteria.Keywords: traditional, pond, nitrification, abundance of bacteri. ABSTRAKTambak sistem tradisional merupakan tambak yang dalam kegiatannya masih menggunakan sistem manajemen sederhana. Hal ini ditandai dengan penerapan teknologi sederhana, dan tingkat produksi relatif rendah. Kegiatan budidaya di tambak tradisional tidak akan terlepas dari proses nitrifikasi dan denitrifikasi, namun demikian proses ini relatif lebih rendah aktivitasnya daripada tambak sistem semiintensif dan intensif. Tujuan dari penelitian ini adalah mempelajari kelimpahan bakteri penghasil senyawa nitrit, amonium seiring dengan perubahan tekstur tanah, dan N-organik pada tanah tambak tradisional. Media pertumbuhan bakteri dikondisikan bebas oksigen (oxygen free nitrogen/OFN method) , sedangkan kelimpahan bakteri dianalisis dengan rumus most porbable number (MPN). Berdasarkan hasil, setiap strata tanah tambak tradisional memiliki jumlah bakteri penghasil nitrit yang relatif sama, yaitu antara 7,08-7,47 Log cfu/g. Peningkatan kelimpahan bakteri penghasil amonium terjadi pada semua strata, yaitu berkisar antara 5,63 Log cfu/g sampai dengan 8,12 Log cfu/g. Dari hari pertama persiapan, tambak tradisional telah memiliki kelimpahan bakteri penghasil nitrit dan amonium yang berlimpah.Kata kunci: tradisional, tambak, nitritifikasi, kelimpahan bakteri.

Toxicity of mercury (Hg) on survival and growth rate, hemato- and histopathological parameters ofOreochromis niloticus Kukuh Nirmala; Yuni Puji Hastuti; Vika Yuniar
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Heavy metals are serious pollutants of the aquatic environment because of their environmental persistence and ability to be accumulated by aquatic organisms. Oreochromis niloticus exposed to 0, 0.16, 0.5, and 1.0 ppm Hg for 30 days. The aim of this study was to determine the influence of mercury in water on survival rate, growth rates, hematological, and histological parameters of Oreochromis niloticus. This study was conducted from Mei to June 2009. The experimental design was arranged in completely randomized design with four treatments and three replications. Stock density was 8 fish/aquarium with mean initial body weight was 15.70±1.13 g. Growth and survival rates of test fish were decreased with increasing the Hg concentration. Red blood cell (RBC) count, haematocrit content, and haemoglobin content decreased when compared to the control. The number of white blood cells (WBC) increased in mercuric treated fish. The results are statistically significant at p<0.05 level. Keywords:mercury, survival and growth rate, hematology, histopathology, Oreochromis niloticus

Nitrogen and phosphorus absorption capability in environmental culture by Taiwan gravestone Anadonta woodiana Lea Yuni Puji Hastuti; Kukuh Nirmala; Tyas Setioaji
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Nitrogen and phosphorus in a water culture are derived from the metabolism of the farmed organism, feed residue and microbial activity in the water. One way of controlling water quality due to waste in the form of nitrogen and phosphorus from aquaculture is to utilize filter feeder organisms of Anadonta woodiana Lea. This study aimed to analysis the effectiveness of the absorption of nitrogen and phosphorus in waters by A. woodiana as filter feeder. A total of 0 (control), five, ten, and 15 gravestone were putted into a tank of 35 L of water derived from Cirata reservoir. Each treatment was given three times repetition. Measurement of total nitrogen and phosphorus in gravestones were performed at baseline and end of treatment. In addition, measurement of total nitrogen and phosphorus in the water tank was measured every ten days. Another measurement is the length and weight of gravestone. Direct observations made on gravestones barnacle survival, pH, dissolved oxygen, temperature, and turbidity every day. Aeration was provided in each aquarium. Based on the results obtained, the survival of gravestone in the density of five gravestones was 93.33%, in ten gravestones density was 90%, while in 15 gravestones was 95%. The percentage reduction in total nitrogen in the water at the end of treatment was 57% for the five gravestones density, and for ten gravestones was 88.2%. The decrease of total phosphorus in water in ten gravestones density was 37.4%, whereas the increase in density of 15 gravestones and total phosphorus water percentage was 26.9%. On the density of ten gravestones, the body weight decreased until to 3.3%, while in the density of 15 gravestones decreased until to 2.5% on day 30. Results of water quality analysis showed that the DO values decreased from baseline to end of treatment (from 5.08 to 4.86 mg/L), the average water temperature from 26.5 to 27.4 °C, pH values 7‒8, and highest turbidity values was obtained in the density of five gravestone. Decrease in the percentage of total nitrogen in the water suggested nitrogen and phosphorus have been absorbted for barnacle growth. Keywords: total phosphorus, total nitrogen,waste,Anadonta woodiana Lea,cultivation

Additional lime of CaO in rearing medium with salinity for growing of catfish Pangasius hypopthalmus Yuni Puji Hastuti; Daniel Djokosetiyanto; Ide Permatasari
Jurnal Akuakultur Indonesia Vol. 11 No. 2 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Catfish juvenile quality improvements sought through the addition of lime to the availability of minerals in the body. Media maintenance is set at 4‰ salinity aims to reduce the level of osmotic work to reduce energy used for osmoregulation and can be allocated to the growth of catfish. Juvenile-sized fish used 2.59±0.02 cm and weights 0.13±0.01 g. Aquarium measuring 20×20×20 cm3 used 15 units, filled with water salinity of 4% as 6 L per unit, and added lime CaO appropriate treatment. During the study, the fish were fed with 28% protein content three times a day with FR 5%. Addition of CaO treatment dose 0 mg/L, 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L result in the survival rate of 100%, 100%, 100%, 96.29%, and 100% (p>0.05). The growth rate of daily weight 5.196%, 5.212%, 6.596%, 5.35% and 5.101% (p>0.05). Long absolute yield significantly different values are 1.451 cm, 1.518 cm, 1.964 cm, 1.71 cm, and 1.546 cm (p<0.05). During the study, the water quality is within the range that can be tolerated by catfish juvenile. The use of lime in the salinity of 4% is suggested as many as 40 mg/L CaO with the turnover of water every day and feeding enough.Keywords: CaO, salinity, growth, Pangasius hypopthalmus

Profile in various organic soil depth shrimp pond, Tambak Inti Rakyat, Karawang Yuni Puji Hastuti; Lena Novita; Tri Widiyanto; Iman Rusmana
Jurnal Akuakultur Indonesia Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTOrganic material in the bottom of the pond is part of the land is a complex and dynamic system, which is sourced from the rest of the feed, plants, and or animals found in the soil that continuously change shape, because it is influenced by biology, physics, and chemistry. This study was aimed to see the profile of organic material consisting of C, N, and C/N ratio and phosphate in different depths of pond with different culture systems. Observation were conducted at Tambak Inti Rakyat, Karawang in traditional, semi-intensive and intensive culture systems. Observation at mangrove area was also observed as control. Sediment samples at the inlet and outlet at three different depths (0‒5 cm, 5‒10 cm, and 10‒15 cm) was taken every 30 days to measure the content of C, N, C/N ratio, and total phosphate. During the 120 day maintenance period could be known that in all pond systems were used (traditional, semi-intensive, and intensive) the concentration of C-organic and organic-N on average was located in the bottom layer which is a layer of 10‒15 cm. The lack of human intervention from ground pond system, the more diverse the type and amount of organic material contained therein.Keywords: organic materials, subgrade, depth, aquaculture systems, long maintenanceABSTRAKBahan organik di dasar tambak merupakan bagian dari tanah yang merupakan suatu sistem kompleks dan dinamis, yang bersumber dari sisa pakan, tanaman, dan atau binatang yang terdapat di dalam tanah yang terus menerus mengalami perubahan bentuk, karena dipengaruhi oleh faktor biologi, fisika, dan kimia. Penelitian ini bertujuan untuk melihat profil bahan organik yang terdiri dari C, N, dan C/N rasio serta fosfat pada kedalaman tambak yang berbeda dengan sistem budidaya yang berbeda pula. Pengamatan dilakukan di Tambak Inti Rakyat Karawang pada sistem budidaya tradisional, semi intensif, dan intensif. Pengamatan di daerah mangrove diamati pula sebagai kontrol. Sampel sedimen di inlet dan outlet pada tiga kedalaman yang berbeda (0‒5 cm, 5‒10 cm, dan 10‒15 cm) diambil setiap 30 hari sekali untuk diukur kandungan C, N, C/N rasio, dan total fosfatnya. Selama 120 hari masa pemeliharaan dapat diketahui bahwa pada semua sistem tambak yang digunakan (tradisional, semi intensif, dan intensif) nilai konsentrasi C-organik dan N-organik rata-rata terletak pada lapisan paling bawah yaitu lapisan 10‒15 cm. Minimnya campur tangan manusia dari tanah sistem tambak maka semakin beragam jenis dan jumlah dari bahan organik yang terkandung di dalamnya.Kata kunci: bahan organik, tanah dasar, kedalaman, sistem budidaya, lama pemeliharaan

Optimum salinity for growth of mangrove crab Scylla serrata seed in recirculation systems Yuni Puji Hastuti; Ridwan Affandi; Mafatih Devi Safrina; Kurnia Faturrohman; Wildan Nurussalam
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT One of the abiotic factors that affects the growth and the survival of crabs is salinity. The optimum salinity media will give maximum impact on mangrove crab Scylla serrata due to the osmoregulation process. This study aimed to examine the effect of salinity on the survival rate (SR) and spesific growth rate (SGR) of mangrove crab through the reaction of physiological condition. The treatments were rearing mangrove crab at the salinity medium of 15 ppt (A), 20 ppt (B), 25 ppt (C), and 30 ppt (D). Result showed that different salinity performed a significant effect (P<0.05) on the survival rate and specific growth rate of the crabs. The low level of stress, shown by the high value of total hemocyte and the low osmotic pressure, has made salinity of 25 ppt was the optimum condition for the mangrove crab rearing. Keywords: salinity, survival, specific growth rate, mangrove crab ABSTRAK Salah satu faktor abiotik yang memengaruhi pertumbuhan dan kelangsungan hidup kepiting adalah salinitas. Salinitas media optimum akan memberikan efek yang maksimal pada kepiting bakau Scylla serrata sehubungan dengan proses osmoregulasi tubuhnya. Penelitian ini bertujuan untuk menguji pengaruh salinitas pada kelangsungan hidup dan laju pertumbuhan spesifik kepiting bakau melalui reaksi kondisi fisiologis. Penelitian ini terdiri atas perlakuan salinitas media 15 ppt (A), 20 ppt (B), 25 ppt (C), dan 30 ppt (D). Hasil penelitian menunjukkan bahwa perbedaan salinitas media pemeliharaan kepiting bakau memberikan perbedaan nyata (P<0,05) pada kelangsungan hidup dan laju pertumbuhan spesifik kepiting bakau. Rendahnya tingkat stres pada salinitas 25 ppt dijelaskan dengan tingginya jumlah total hemosit dan rendahnya tekanan osmotik sehingga salinitas 25 ppt merupakan kondisi optimum bagi pemeliharaan kepiting bakau. Keywords: salinitas, kelangsungan hidup, laju pertumbuhan spesifik, kepiting bakau

Addition of CaCO3 to culture media at the salinity of 3 g/L for freshwater tambaqui growth Yuni Puji Hastuti; Chandra Yudistira; Kukuh Nirmala; Wildan Nurusallam; Kurnia Faturochman
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Increasing of freshwater tambaqui Colossoma macropomum demand makes the farmers increase the production of the consumption fish and seed. Acceleration of the production cycle can increase total production level, and reduce the level of osmotic work can be used to improve fish growth. This study aimed to analyze the effect of the addition of calcium carbonate (CaCO3) in the saline medium of 3 g/L on the growth of freshwater tambaqui juvenile. Tambaqui used has a body length of 1.93 ± 0.1 cm and weight of 0.26 ± 0.03 g. The experiment used 15 units of aquarium at size of 30×15×25 cm3 and filled with 9 L of saline water, then added lime CaCO3 according to treatment. The treatments were control (0 mg/L CaCO3), A (50 mg/L CaCO3), B (100 mg/L CaCO3), C (150 mg/L CaCO3), and D (200 mg/L CaCO3). The study was conducted for 30 days of maintenance. Fishes were fed on bloodworms ad libitum or provided three times a day. The results showed that survival, daily growth rate, and absolute length growth of the CaCO3 treatments significantly higher (P<0.05) that that of control. Furthermore, survival, daily growth rate, and absolute length growth among the CaCO3 treatments were the same. Thus, addition CaCO3 of 50 mg/L saline water of 3 g/L can be applied to increase culture performance of freshwater tambaqui. Keywords: freshwater tambaqui, CaCO3, salinity ABSTRAK Permintaan terhadap ikan bawal air tawar Colossoma macropomum yang semakin meningkat membuat pembudidaya menambah produksi ikan konsumsi dan benih. Percepatan siklus produksi dapat meningkatkan total produksi budidaya, dan energi dari optimasi kerja osmotik dapat dialokasikan untuk pertumbuhan. Penelitian ini bertujuan untuk menganalisis pengaruh penambahan kalsium karbonat (CaCO3) pada media bersalinitas terhadap pertumbuhan benih ikan bawal air tawar. Benih ikan bawal yang digunakan memiliki panjang 1,93±0,1 cm dengan bobot 0,26±0,03 g. Akuarium yang digunakan berukuran 30×15×25 cm3 sebanyak 15 unit dan diisi air bersalinitas 3 g/L sebanyak 9 L, kemudian ditambahkan kapur CaCO3 sesuai perlakuan. Dosis setiap perlakuan terdiri atas kontrol (0 mg/L CaCO3), A (50 mg/L CaCO3), B (100 mg/L CaCO3), C (150 mg/L CaCO3), dan D (200 mg/L CaCO3). Penelitian dilakukan selama 30 hari pemeliharaan dengan pemberian pakan cacing sutra secara ad libitum atau diberikan tiga kali sehari. Hasil penelitian menunjukkan bahwa derajat kelangsungan hidup, laju pertumbuhan harian, dan panjang mutlak pada perlakuan penambahan kapur CaCO3 lebih tinggi (P<0,05) daripada kontrol. Sementara itu, derajat kelangsungan hidup, laju pertumbuhan harian, dan panjang mutlak antarperlakuan penambahan kapur CaCO3 tidak berbeda. Dengan demikian, penambahan CaCO3 sebanyak 50 mg/L air dapat diterapkan untuk perbaikan performa budidaya ikan. Kata kunci: ikan bawal air tawar, CaCO3, salinitas

Co-Authors . Alimuddin . Zairion Agus Oman Sudrajat Ali Mashar Alina Akhdiya Arul Tabah Prastomo Atma Jaya Salman Muin Bianingrum Bianingrum Chandra Yudistira Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto DEDI JUSADI Dinamella Wahjuningrum Dudi Muhammad Wildan Eddy Supriyono Eni Kusrini Fauzan Wahib Alsani Haris luthfi Hasan Nasrullah Horas Nadeak I. Effendi Ide Permatasari Iis Diatin Iman Rusmana Irzal Effendi Izhar Amirul Haq K. Nirmala Kukuh Nirmala Kurnia Faturochman Kurnia Faturrohman Lena Novita Mafatih Devi Safrina Mia Setiawati Mia Setiawati Mia Setiawati Moh. Burhanuddin Mahmud Naila K Aini Odang Carman Peter Funch Priyo Handoyo Wicaksono Putri Utami Radhita Millaty Rani Nuraisah RIDWAN AFFANDI Ridwan Affandi Rina Hesti Utami Rizal Khoirun Alfisah Siska Tridesianti Siska Tridesianti Sri Nuryati Sulistia Wardani Syamsul Bahri Agus, Syamsul Bahri T. Widiyanto Tatag Budiardi Thoy Batun Citra Rahmadani Tri Widiyanto Tyas Setioaji Vika Yuniar Wahyu Budi Kuntari Wijianto Wijianto Wijianto Wijianto Wildan Nurusallam Wildan Nurusallam Wildan Nurusallam Wildan Nurussalam, Wildan Y. Hadiroseyani Yonvitner - Yuli Siti Fatma Yuli Siti Fatma Yusli Wardiatno

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