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Pendugaan Nilai Heritabilitas Bobot Lahir dan Bobot Sapih Domba Garut Tipe Laga A Gunawan; R R Noor
Media Peternakan Vol. 29 No. 1 (2006): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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The objective of this study was to estimate heritability of birth and weaning weights of the fighting type of Garut sheep. The data of birth and weaning weight of 175 SR (Super lambs, i.e. cross between fighting type Garut rams and selected Garut ewes) and 61 SB (Sukabumi lambs, i.e. cross between fighting type Garut rams and Sukabumi ewes) were used. The data were collected from March 2001 to August 2002. The results showed that the means of all traits of SR sheep group were larger (P < 0.01) than those of SB sheep group. Estimated heritability of birth weight and weaning weight of SR sheep were 0.67 ± 0.19 and 0.95 ± 0.16 respectively. Estimated heritability value of birth and weaning weight of SB sheep were 0.53 ± 0.33 and 0.57 ± 0.37 respectively. The heritability of birth and weaning weight were considered as high which means that the selection programme will be more effective and efficient in improving the genetic merits. Key words : heritability, birth and weaning weight, fighting type Garut sheep

Evaluasi Keragaman Genetik Gen Hormon Pertumbuhan (GH) pada Sapi Pesisir Sumatera Barat Menggunakan Penciri PCR-RFLP . Jakaria; D Duryadi; R R Noor; B Tappa; H Martojo
Media Peternakan Vol. 30 No. 1 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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A total of 134 Pesisir cattle were genotyped for growth hormone (GH) gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype and allele frequencies of the GH MspI and GH AluI Pesisir cattle were determined. The GH MspI gene frequencies for the C and T allele were 0.209 and 0.791 respectively, while GH AluI gene frequencies for the L and V allele were 0.992 and 0.008 respectively. The chi-square analysis indicated that this population is not in Hardy-Weinberg Equilibrium status. Expected heterozygosis value (He) for GH MspI and GH AluI were 0.3306±0.0266 and 0.0149±0.0073 respectively. The PCR-RFLP GH MspI marker has higher genetic variability compare to PCR-RFLP AluI marker. This finding showed that GH MspI T allele was favorable as a GH marker for Bos indicus breeds. Key words: Pesisir cattle, growth hormone gene, PCR-RFLP, polymorphism

Identifikasi Keragaman Genetik Gen Reseptor Hormon Pertumbuhan (GHR|Alu I) pada Sapi Bali Zulkharnaim Zulkharnaim; . Zakaria; R R Noor
Media Peternakan Vol. 33 No. 2 (2010): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Growth hormone receptor (GHR) is one factor affecting animal growth. GHR is required by growth hormone (GH) to carry out its effects on target tissues. The objective of the study was to estimate genetic diversity of the GHR|AluI gene in bali, limousin, simmental and pesisir cattle. Genotyping was performed on 248 animals, including 162 bali, 21 limousin, 17 simmental and 48 pesisir. Single nucleotide polymorphisms (SNP) had been found in exon 10, coding for the cytoplasmic domain of GHR, which was located at position 81 bp (A/G) induced amino acid substitutions Ser/Gly. Genotype frequencies of bali cattle AA (0.988), GG (0.006) and AG (0.006) were evidenced for the GHR AluI monomorphism, but mostly different from limousin GG (0.667), AA (0.238) and AG (0.095), simmental AG (0.529), GG (0.471) and AA (0.000), pesisir AA (0.604), GG (0.375) and AG (0.021) were the evidence of polymorphism. Homozigosity (monomorphism) in bali cattle might be affected by adaptability in extreme environmental conditions such as poor nutrition and improper management practices. It also could be affected by natural selection and phenotype plasticity phenomena.

Characterization of Partial Coding Region Fibroin Gene on Wild Silkmoth Cricula trifenestrata Helfer (Lepidoptera: Saturniidae) Suriana Suriana; D D Solihin; R R Noor; A M Thohari
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The study was conducted to characterize coding region of wild silkmoth C. trifenestrata partial fibroin gene, and detect these gene potential as molecular marker. A total of six larvae C. trifenestrata were collected from Bogor, Purwakarta and Bantul Regency. Genomic DNA was extracted from silk gland individual larvae, then amplified by PCR method and sequenced. DNA sequenced result was 986 nucleotide partial fibroin gene of C. trifenestrata, which are comprising complete coding region of first exon (42 nucleotide), an intron (113 nucleotide), and partial of second was exon (831 nucleotide). Only coding region was characterized. Results showed that first exon very conserved in C. trifenestrata. These gene consisted of 31%, thymine, 28% guanine, 21% cytosine, and 19% adenine. Cytosine and thymine (sites of 25th and 35th respectively) were marker for C. trifenestrata species. The first exon encoding 14 amino acids. Valine amino acid (12th site) was marker to the species C. trifenestrata. The partial second exon consisted of guanine (32.7%), alanine (26.5%), thymine (21%) and cytosine (19.7%). These region encoded 277 amino acids, which were dominated by the alanine (27.8%) and glycine (21.66%). Alanine formed polyalanine sequence with different motifs namely: AAAAAAASS, AAAAAAAAAAAGSSG, AAAAAAAAAAAAGSGTGFGGYDS, AAAAAAAAAAGSSGRGGYDGVDGGYGSGSS, and AAAAAAAAAAAAGSSGRGLGGYDGWVDDGYGSGSGS.

Morphometric Characterization of Minahasa Horse for Breeding and Conservation Purposes B J Takaendengan; R R Noor; S Adiani
Media Peternakan Vol. 34 No. 2 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this study was to investigate morphometric variation of Minahasa horses. Body measurements and live-weight were taken on 195 mare and 310 stallion of Minahasa horse. A multivariate approach was adopted to provide description of both body shape and body size of four Minahasa local horse populations, i.e. Tomohon, Manado, South Minahasa, and Minahasa. Statistical methods employed in this study were general linear model, simple discriminant analysis, and principle component analysis were used to construct phylogenic trees. The results showed that Tomohon’s horse population had higher body weights and body measurements (P<0.05) than those from three other areas (Manado, South Minahasa, and Minahasa). The hip width is the most discriminant variable to determine the differences among Minahasa local horse population. The results support establishment of strategy to promote the use and the development of local adapted horse.

Morphometric and Phylogenic Analysis of Six Population Indonesian Local Goats A Batubara; R R Noor; A Farajallah; B Tiesnamurti; M Doloksaribu
Media Peternakan Vol. 34 No. 3 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The research objectives were to characterize morphometric and genetic distance between populations of Indonesian local goats. The morphological discriminant and canonical analysis were carried out to estimate the phylogenic relationship and determine the discriminant variable between Benggala goats (n= 96), Marica (n= 60), Jawarandu (n= 94), (Kacang (n= 217), Muara (n= 30) and Samosir (n= 42). Discriminant analysis used to clasify body weight and body measurements. In the analysis of variance showed that body weight and body measurement (body length, height at withers, thorax width, thorax height, hert girth, skull width and height, tail length and width, ear length and width) of Muara goats was higher (P<0.05) compared to the other groups, and the lowest was in Marica goats. The smallest genetic distance was between Marica and Samosir (11.207) and the highest were between Muara and Benggala (255.110). The highest similarity between individual within population was found in Kacang (99.28%) and the lowest in Samosir (82.50%). The canonical analysis showed high correlation on canon circumference, body weight, skull width, skull height, and tail width variables so these six variables can be used as distinguishing variables among population. The result from Mahalonobis distance for phenogram tree and canonical analysis showed that six populations of Indonesian local goats were divided into six breed of goats: the first was Muara, the second was Jawarandu, the third was Kacang, the fourth was Benggala, the fifth was Samosir and the sixth was Marica goats. The diversity of body size and body weight of goats was observed quite large, so the chances of increasing productivity could be made through selection and mating programs.

Genetic Polymorphisms of the Coding Region (Exon 6) of Calpastatin in Indonesian Sheep M I.A Dagong; C Sumantri; R R Noor; R Herman; M Yamin
Media Peternakan Vol. 34 No. 3 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Calpastatin (CAST) is an indigenous inhibitor of calpain that involved in regulation of protein turn over and growth. The objective of this research was to identify genetic polymorphisms in the entire exon 6 of calpastatin gene in Indonesian local sheep. A PCR-SSCP method was carried out to identify genetic variation of CAST gene. In total 258 heads of local sheep from 8 populations were investigated, three groups of samples were Thin Tail Sheep (TTS) from Sukabumi, Jonggol, and Kissar. The rest samples were Priangan sheep (PS) from Margawati (Garut meat type) and Wanaraja (Garut fighting type) and Fat Tail Sheep (FTS) from Donggala, Sumbawa, and Rote islands. SSCP analysis revealed that three different SSCP patterns corresponded to three different alleles in the CAST locus (CAST-1, 2, and 3 allele) with five different genotypes. Genetic variation between local sheep populations were calculated based on genotypic and allelic frequencies. Most populations studied were polymorphic, with genotype frequencies of CAST-11, CAST-12, CAST-22, CAST-32, and CAST-33 were 0.286, 0.395, 0.263, 0.046, and 0.007 respectively. CAST-1 and 2 alleles were most commonly found in all populations with total frequency was 0.970, while CAST-3 was a rare allele 0.030 and only found in TTS population. Variation in the CAST gene could be used for the next research as genetic diversity study or to find any association between CAST polymorphism with birth weight, growth trait and carcass quality in Indonesian local sheep.

Live Weight Estimation by Chest Girth, Body Length and Body Volume Formula in Minahasa Local Horse B J Takaendengan; U Paputungan; R R Noor; S Adiani
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Study was conducted in the regency of Minahasa to estimate horse live weight using its chest girth, body length and body volume formula (cylinder volume formula) represented by animal chest girth and body length dimensions, particularly focused in Minahasa local horses. Data on animal live weight (LW), body length (BL), chest girth (CG) and body volume were collected from 221 stallions kept by traditional household farmers. Animal body volume was calculated using cylinder volume formula with CG and BL as the components of its formula. Regression analysis was carried out for LW with all the linear body measurements. The data were classified on the basis of age. Age significantly (P < 0.05) influenced the body measurements except animal body length (P>0.05). Animal live weight was predicted by simple regression models using dependent variable (Y) of the animal live weight and independent variable (X) of the animal body measurement, either body length, chest girth, or body volume. The correlations between all pairs of measurements were highly significant (P < 0.01) for all age groups. Regression analysis showed that live weight could be predicted accurately from body volume (R2= 0.92) and chest girth (R2= 0.90). Simple regression model that can be recommended to predict horse live weight based on body volume with their age groups ranging from 3 to ≥10 years old was as follow: Live weight (kg)= 5.044 + 1.87088 body volume (liters). The analyses of data on horse chest girth, body length and body volume formula provided quantitative measure of body size and shape that were desirable, as they enable genetic parameters for these traits to be estimated and also included in breeding programs.

The Genetic Diversity of TLR4 MHC-DRB Genes in Dairy Goats Using PCR-RFLP Technique M Petlane; R R Noor; R R.A Maheswari
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This research was aimed at evaluating the genetic polymorphism of TLR4 and MHC-DRB genes in dairy goats [(Saanen, Etawah Grade-Saanen Crossbred (PESA), and Etawah Grade (PE)] using PCR -RFLP. The two genes are involved in immunity where they play a crucial role in pathogens recognition and presentation to T-cells and CD4 cells. PCR was used to amplify genomic DNA for TLR4 (382 bp) and CaLA-DRB (285 bp) genes fragments. Genetic polymorphism was detected by digesting TLR4 amplimer with AluI while DRB amplimers were digested with PstI and TaqI in two separate reactions. The results showed that TLR4|AluI was monomorphic and fixed with allele T in all three breeds while DRB|TaqI and DRB|PstI loci were found polymorphic for all breeds. Heterozygosity expected (He) and PIC were found low at both DRB|TaqI and DRB|PstI loci in PE and Saanen. Χ2 results showed that DRB|PstI in PE and DRB|TaqI in PESA were not in H-W equilibrium and did not display homozygous recessive genotype. The results declared that TLR4|AluI was not a good for marker for diseases resistance whereas DRB|TaqI and DRB|PstI gave hope for resistance based on their PIC.

The Characteristics of Cytochrome C Oxidase Gene Subunit I in Wild Silkmoth Cricula trifenestrata Helfer and Its Evaluation for Species Marker . Suriana; D D Solihin; R R Noor; A M Thohari
Media Peternakan Vol. 35 No. 2 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The study was conducted to assess the characteristics of partial gene of cytochrome C oxidase subunit I (COI) of wild silkmoth Cricula trifenestrata, and to detect the diagnostic sites from these gene for evaluation as species marker. A total of fifteen larvae of C. tifenestrata were collected from Bogor, Purwakarta, and Bantul Regencies. Genomic DNA was extracted from silk gland of individual larvae, then amplified by PCR method and sequenced. DNA sequencing was done to characterize their nucleotide and amino acid contents. The results showed that 595 nucleotides at the 5 'end of COI gene of C. tifenestrata was conserved at the species level, but varies at the family level. Nucleotide dominated by thymine and adenine bases (± 70%). There were 25 diagnostic sites for C. tifenestrata, and four diagnostic sites for genus level. One hundred eigthty nine (189) amino acids were alignment, and only one percent of the genes was varied among species. The 107th amino acid (valine) and 138th(threonine) were diagnostics amino acid for C. tifenestrata. Based on nucleotides and amino acids sequences, the phylogeny showed that C. tifenestrata lied on the same nodes with Antheraea, so the Saturniidae family is monophyletic.

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