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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology
. Yulnawati
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Agriculture, Biological Sciences & Forestry

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Kualitas Semen Cair Asal Epididimis Kerbau Belang dalam Bahan Pengencer Andromed yang Mendapat Penambahan Sukrosa M Surachman; . Herdis; . Yulnawati; M Rizal; H Maheshwari
Media Peternakan Vol. 32 No. 2 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.972 KB)

Abstract

The aim of this research was to observe the quality of spotted buffalo epididymal sperm after storage at 4 °C as liquid semen for 12 and 24 hours in andromed containing sucrose 0.2% and 0.4%. Sperm was collected from epididymal tissues by combination of slicing and pressing methods. The parameters observed were the percentage of progressive motility and live sperm. The results showed that the percentage of progressive motility of the liquid semen after storage for 12 hours in andromed (A), andromed + sucrose 0.2% (P1) and andromed + sucrose 0.4% (P2) were 48.33%; 53.33% and 55% (P>0.05), respectively. In addition, the percentage of motility after 24 hours of storage in those extenders were 45%; 46.67% and 46.67% (P>0.05), respectively. The percentage of live sperm in A, P1 and P2 after 12 hours of storage were 70.33%; 73% and 73.33% while after 24 hours of storage, the percentage of live sperm in those extenders were 66.33%; 68.67% and 69.67%, respectively. In conclusion, the addition of 0.2% and 0.4% sucrose in andromed extender could maintain the quality of the spotted buffalo epididymal sperm after storage for 12 and 24 hours at 4 °C. Key words: epididymal sperm, spotted buffalo, sucrose, liquid semen, andromed
Profiles of Cortisol, Triiodothyronine, Thyroxine and Neutrophil/Lymphocyte Ratio as Stress Indicators in Swamp Buffaloes 15 Days Post-Transportation H Maheshwari; . Yulnawati; A Esfandiari; . andriyanto; M D Andriani; A Khovifah
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (914.602 KB) | DOI: 10.5398/medpet.2013.36.2.106

Abstract

Transportation may cause stress that affects livestock’s health. This research was conducted to observe the effect of transportation on the profiles of blood cortisol, triiodothyronine (T3), thyroxine (T4) and neutrophil/lymphocyte (N/L) ratio as stress indicators during 15 days post-transportation. Four females swamp buffaloes, 2-yr-old were used in this research. The animals were transported using an open truck, along 15 kilometers distance for 2 h. During acclimatization, the animals were fed grass twice a day and access to water ad libitum. Blood was collected at the time of arrival and everyday for 15 d post-transportation. Cortisol, T3 and T4were analyzed using Radioimmunoassay (RIA) method. Blood smears were also prepared and stained with Giemsa for leukocyte differential counts. Results showed a significant relation (P<0.05) between transport and an increase in cortisol and T4 level on day-1 post-transportation compared to the normal levels. The level of T3 was also increased on day-3 post-transportation. Total leukocyte counts were 8.91-18.83×10³/μL. Neutrophil, eosinophil, monocyte, and lymphocyte counts were 4.87-10.41×10³/μL, 0.66-1.75×10³/μL, 0.11-0.58×10³/μL, and 2.98-6.21×10³/μL, respectively. N/L ratio was 1.23-3.49 with >1.5 in average throughout the period of the research. It is concluded that transportation causes stress and therefore changes metabolic process. 
Co-Authors . andriyanto . Herdis A Esfandiari A Khovifah Andriyanto Andriyanto H Maheshwari M D Andriani M Rizal M Surachman