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Pertumbuhan Tunas Tacca leontopetaloides L. Hasil Mikropropagasi Setelah Pemberian Radiasi Sinar Gamma Co60 dan Hormon Tumbuh yang Berbeda Darnia Astari Parastiti; Endang Kusdiyantini; Endah Dwi Hastuti; Betalini Hapsari; Tri Muji Ermayanti
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.763 KB)

Abstract

Taca  (Tacca  leontopetaloides  L.)  is  one  of  the  plant  family Dioscoreaceae  that  contain compounds   glikosida   flavonoid   called   taccalin   and   taccalonoides   who   potential   as antioxidants. Taka (Tacca  leontopetaloides L.) specifically has not been cultivated, grown only a limited area around the beach, it is necessary to plant vegetative propagation system faster with more results in tissue culture systems. This study aims to determine the growth and the antioxidant potential shoots Tacca leontopetaloides L. mikropropagasi results after administration  of  Co60  Gamma  ray  radiation  and  different  growth  hormones.  Research method  uses  completely  randomized  factorial  design  (RAL)  with  4  treatment  doses  of Gamma radiation Co60 is 0 Gy, 5 Gy, 20 Gy, and 30 Gy and 3 treatment of growth hormone that MSo, MSo + 0,5 BAP and MSo + 0,5 Kinetin with 3 replicates. Shoots taka from results mikropropagasi grown in  vitro and has been irradiated with Co60 Gamma rays. Parameters tested were growth of shoots, amount of leaves, till, roots and wet weight taka. The results showed  that  Gamma radiation  Co60  and  plant  regulator  cytokinin  effect  on  shoots  taka, amount of tillers, leaf, root and wet weight taka. Keywords: Taka (Tacca leontopetaloides L.), Mikropropagation, Growth Hormone.
Karakter Anatomi Daun dari Kultur Tunas Artemisia annua L. , Juliarni; Hamami Alfasani Dewanto; Tri Muji Ermayanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.067 KB) | DOI: 10.24831/jai.v35i3.1336

Abstract

Artemisia annua L. produce artemisinin, an endoperoxide sesquiterpene lactone, which is effective against resistant strains of Plasmodium falciparum, the malarial parasite. Artemisinin in foliar tissue are localized entirely in subcuticular space of capitate glandular trichomes. This research was performed to investigate the anatomical structures especially glandular trichomes which associated with artemisinin production in leaves of five different shoot culture clones (A, B, C, D, and E clones). Observation of anatomical characters of leaves was done by making cross-section, while observation of trichomes was performed using Scanning Electron Microscopy.  The leaves of five clones showed bifacial anatomical structure.  The leaf thickness of E clone was the highest (96.8 µm), while those of four other clones were relatively the same ranging from 62.8 µm to 66.6 µm. Glandular trichomes were distributed throughout the lamina of leaves with the highest distribution in adaxial parts of  the leaves. The size of uppermost secretory cells of glandular trichomes was relatively the same in five clones observed. There were variations in density of  glandular  trichomes in five clones observed. A and B clones had higher density of glandular trichomes i.e. 56.9 and 60.5/mm2, while three other clones had density which range from 43.0 to 49.7/mm2. It was suggested that A and B clones were the potential clones in producing artemisinin in vitro due to their larger leaf size and higher density of glandular trichomes.   Keywords :  Artemisia annua, shoot culture, anatomical structure of leaf
Pertumbuhan Bibit Tanaman Manggis (Garcinia mangostana L.) Setelah Inokulasi dengan Berbagai Galur Agrobacterium rhizogenes1 , Lizawati; Roedhy Poerwanto; , Sobir; Iman Rusmana; Tri Muji Ermayanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 35 No. 2 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.098 KB) | DOI: 10.24831/jai.v35i2.1321

Abstract

Growth of mangosteen essentially depends on its root system.  Therefore, it needs technology to obtain stringer mangosteen root system.  The use of Agrobacterium rhizogenes bacterium is an alternative.  The objectives of this experiment were : 1) to find the effective strain of A. rhizogenes bacterium for inoculation of mangosteen seedling root, 2) to find the best inoculation method for inducing mangosteen seedling root.  The materials used in this experiment were ; mangosteen fruit and A. rhizogenes collection from Puslit Biotechnology LIPI Cibinong-Bogor.  The experiment was arranged in completely randomized design with two factorial treatments.  The first factor : 11 strains A. rhizogenes (ATCC-15834, ATCC-8196, R-1000, 07-20001, A4, A4-J, 509, 510, 511, MAFF 01-1724, and control), the second factor : 2 inoculation methods (cutting and dipping).  The results showed that A. rhizogenes  of ATCC-15834, 509, 07-20001, A4, and R-1000 increased stem diameter, plant height, leaf number, lateral and tertiary root number, better than ATCC-8196, MAFF 01-1724, 510, 511, A4-J, and control.  Cutting root method of inoculation resulted in higher live plant percentage compared to dipping root method.   Key words :  Agrobacterium rhizogenes, Garcinia mangostana, inoculation
Enkapsulasi dan Regenerasi Kalus Embriogenik Mangga (Mangifera indica L.) Kultivar Bapang dan Gadung 21 Tri Muji Ermayanti; Robertus Nugroho; Hamim Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2598

Abstract

The mother plant and genetic variability of Indonesian mango need to be conserved. Encapsulation is one of in vitro conservation used for many plant species. The aim of this research was to study the regeneration of encapsulated mango cultivar Bapang and Gadung 21 embryogenic callus after storage at -14, 3−5, and 26−27oC for 0, 1, 2, 4, and 8 weeks. Embryogenenic callus was treated with 3% Na-alginate (in liquid 3M), then it was dropped into 100 mM CaCl2. Encapsulated callus beads were dehidrated and stored at -14, 3−5, and 26−27oC for 0, 1, 2, 4, and 8 weeks. After storage, the callus was cultured in 3M medium containing 2,4-D at 0, 1, and 2 mg/l for the regeneration. The results showed that after 8-week storage, callus of Bapang culticar was more viable (67.3%) and resulted more number of somatic embryos (191.6%) than Gadung 21 cultivar. The callus which was cultured in 3M medium without addition of 2,4-D was more viable (20.9%) and had more number of somatic embryos (1062.5%) than that which was cultured on medium containing 2,4-D. After 2-week storage, callus had viability of 7.6%. No storage callus formed more somatic embryos than storage callus. Storage at 26−27oC gave higher viability as well as higher number of somatic embryo than stored at -14 and 3−5 oC. The callus did not regenerate into shoots after storage at -14oC. Embryogenic callus could be stored at 3−5 and 26−27oC for 4 weeks.
Pertumbuhan dan Variasi Jumlah Kromosom Akar Rambut Morus macroura Miq. Hasil Transformasi dengan Beberapa Galur Agrobacterium Tri Muji Ermayanti; Dwi Hastuti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2687

Abstract

Morus macroura Miq. (Moraceae) which is native West Sumatra is now classified as endangered species and usually used as furniture. This plant produces phenolic compounds. Generally secondary metabolites produced by plants are found in low level, therefore, in vitro techniques such as callus culture, cell suspension and organ cultures are the alternative methods to increase their in vitro production. However, problems on genetic instability such as variation in chromosome numbers and abnormality of chromosome structure are found. These cases influence the productions of secondary metabolites. Genetic variation could be overcome using hairy root culture. The aim of the research was to analyze the growth and chromosome numbers of Morus macroura Miq. hairy root transformed with Agrobacterium strains TISTR-511, TISTR-512, ATCC-15834 and R-1000 in order to determine the genetic variation of the culture. Growth was determined by measuring the fresh and dry weights of roots after 4 weeks in culture. Chromosome numbers was prepared by squashing. The results showed that transformed roots had higher growth compared to the growth of untransformed roots. The growth of hairy roots was varied depending on the strains of Agrobacterium. Both transformed and untransformed roots had high variation in the chromosome numbers. Genetic stability of both transformed and untransformed roots were low but they had similar pattern of distribution on the chromosome number. The roots had the diploid number of chromosome 2n=2x=28 ranged from 35.83 to 46.13% and the tetraploid numbers 2n=4x=56 ranged from 23.23 to 42.21%. Total cells examined were more that 230 cells.
Analisis kandungan artemisinin pada kultur tunas Artemisia annua L. dengan lima karakter morfologi yang berbeda Tri Muji Ermayanti; Erwin Al Hafiizh; Aryanti Aryanti; Lenny Sutedja
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2875

Abstract

Artemisia annua L. is a medicinal plant that has an antimalarial drug againts Plasmodium falciparum strains which are resistant to traditional drugs such as quinine and chloroquine. This plant contains artemisinin as major compounds found on its leaves. Aim of the research was to analyze the artemisinin content of shoot culture of A. annua which has five different in leaf morphologies (Morphology type A, B, C, D and E). Previous investigation showed that shoot regenerated from callus has five different morphologies. This somaclonal variation was permanent after continuously subcultured onto MS medium with no addition of plant growth regulators. Artemisinin was detected using HPLC and it was comfirmed by LC-MS. The results showed that shoot culture type B contained the highest artemisinin content (0.171%) amongst other type of morphology. Shoot type D showed the lowest level of artemisinin (0.006%) which was not significantly different with type E (0.008%). Addition of BAP at concentration of 1 and 2 mg/l increased artemisinin level of the shoot culturs.
Struktur Anatomi Daun Artemisia cina Berg. Ex Poljakov Hasil Kultur Jaringan Tri Muji Ermayanti; Juliarni Juliarni; Yuli Andry
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.2911

Abstract

This study was conducted to investigate the anatomical structure of leaves of Artemisia cina Berg. ex Poljakov due to variances in leaf morphology during grown in vitro. Shaking of culture, position of stem nodes used as explants and concentration of BAP (Benzyl Amino Purin) added to the culture medium were the factors investigated in this study. There was variation in leaves morphology of culture, however this variation did not occur after aclimatization and plant grown in the greenhouse. BAP decreased the level of chlorophyll content of leaves. In general, anatomical characters of leaves were not influenced by shaking, nodes position of stem and level of BAP.