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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia Zuriat

G. A. Wattimena

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Author-ID : 2427955

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Education

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Transformasi Tanaman Tebu (cv. PSJT 94-41) dengan Gen Fitase Menggunakan Agrobacterium tumefaciens GV 2260 (pBinPI-IIEC) *] , Susiyanti; G. A. Wattimena; M. Surahman; A. Purwito; D. A. Santosa
Indonesian Journal of Agronomy Vol. 35 No. 3 (2007): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Phosphorus is a critical nutrient for the growth and development of sugarcane in the marginal land in Indonesia. P stored in plant as phytic acid (myo-inositolhexakisphosphate). The objective of the study was to increase activity of phytases enzyme in sugarcanes (cv. PSJT 94-41) through phytase gene transformation. Detection of chimeric gene by PCR showed that the phytase gene was integrated into the genome of sugarcanes. Transformation caused some abnormality such as albino, discoloration, lack of chlorophyll in the particular spot of leaves. Putative transgenic plantlets expressed a higher levels of phytase enzyme activity (38.1 %), whereas increase in P available in plant (19.5 %) and content of chlorophyll (32.3 %). Key words: Sugarcane, transformation, phytase, Agrobacterium tumefaciens

Transformasi Genetik Tanaman Kentang cv. Atlantik Dengan Mengintroduksikan Gen Hordothionin untuk Mendapatkan Ketahanan terhadap Penyakit Bakteri , Nurhasanah; G. A. Wattimena; Agus Purwito; Ni Made Armini Wiendi; , Suharsono
Indonesian Journal of Agronomy Vol. 31 No. 2 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Hordothionins are small anti-bacteria proteins present in barley endosperm, To reveal the potential of this proteins for engineering bacterial disease resistance into potato, a semi-synthetic hordothionin gene construct was introduced in potato cv. Atlantic via Agrobacterium tumefaciens strain LBA 4404, under the control of a cauliflower mosaic virus (CaMV) 358 promotor. The in vitro grown stem (internodus) was used in this research. After 6 weeks in regeneration medium and 2 weeks in rooting medium there were 22 regenerated plants that were screened in kanamycine containing medium. PCR analysis using spesific primer from CaMV 358 promotor showed the presence of amplified T-DNA in 4 transgenic lines from 22 putative transgenic plants were tested, The in vitro toxicity against Ralstonia solanacearum tested from transgenic lines showed variation in resistance level, There were only 2 of the transgenic lines were tolerant, while one of them was moderate tolerant even one of them was susceptible. Key words: Potato, Hordothionin gene, Disease resistance

Perakitan Kultivar Kentang Unggul Indonesia secara Cepat dengan Metode Turunan Klonal Biji Tunggal dan Pra - Evaluasi Secara In Vitro G. A. Wattimena; Agus Purwito; H . M. Machmud; , Samanhudi
Indonesian Journal of Agronomy Vol. 29 No. 3 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

At least ten years needed to abtain new potato cultivar through sexual hybridization, somatic hybridization or through genetic transformation, To short cut this process, Laboratory of Biotechnology, Department of Agronomy, IPB employed a strategy so called single seed in vitro clonal decent ( SSICD) by usings elected parental lines for TPS (True Potato Seed) production. This breeding consist of in vitro pre evaluation for resistance wilt, fusarium wilt, black leg, rot knot nematode and maturity. Using the same number of bacterial cell / (109 cell/ml), there were positive correlation between in vitro test for disease resistance through dripping test or dripping test with greenhouse test through direct inoculation of Ralstonia solanancearum. Resistant clones to fusarium wilt and verticillium were also resistant to bacterial wilt. In vitro tuberization could be use to evaluate maturity of potato cultivar. Key words: Potato, SSICD

ISOLASI DAN REGENERASI PROTOPLAS DARI MESOFIL DAUN KENT ANG (Solanum tuberosum L) DIHAPLOID Agus Purwito; G. A. Wattimena; Antonius Suwanto; Inez H.S. Loeddin Suharsono; Hajrial Aswidinnoor
Indonesian Journal of Agronomy Vol. 27 No. 1 (1999): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid cultivars of potato BF 15 and SVP 10 leaves used four different enzymes solution. Protoplast were cultured onto four different cultures media to increase plating efficiency. Calli were then transferred to ten different regeneration media. Using cellulose RS 0.5 % and pectolyase Y-23 0.05 % protoplast yield of both cultivars were improved. Medium VKM supplemented with 0.2 mg/l 2, 4-D, 1.0 mg/l NAA and 0.5 mg/l zeatin or 2iP were increase recovery of colonies from protoplast up to 5.9%. Regeneration medium containing zeatin did always produce more shoots than those of 2iP. Genotypes dependant regeneration frequencies have also been showed in this experiments

PERBANYAKAN IN VITRO TANAMAN BAWANG PUTIH (Allium sativum L.) VARIETAS LUMBU PUTIH MELALUI INDUKSI TUNAS ADVENTIF Ni Made Armini Wiendi; G. A. Wattimena; Enny Prasetyanti
Indonesian Journal of Agronomy Vol. 24 No. 1 (1996): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Two sets of experiments were conducted to determine the effect of growth hormones, argynine, and coconut water, on the adventitious shoot induction from garlic tissue, and also to find out the best medium for adventitious shoots proliferation. Both experiments could induce direct adventitious shoot and indirect adventitious shoot formation, from calli. Medium with 2 ppm Kinetin and 0.4ppm 2,4-Dproduce good quantity and quality of shoots. The number of shoot from this medium were 32.6 shoots per explant. Medium with 0.5 ppm Kinetin, 0.1 ppm 2.4-D, 25 ppm Argynine, and 10% coconut water produce the highest diameter and good quality of calli, while medium with 1 ppm 2iP and 25 ppm Argynine induced adventitious shoot from calli and produced the highest number of shoot per culture (33.9 shoots).

PENJARANGAN BUAH ANGGUR "BS-6" DENGAN PEMETIKAN BUAH DAN PEMOTONGAN TANDAN Winarso D. Widodo; Sri Setyati Harjadi; G. A. Wattimena; A. A. Mattjik
Indonesian Journal of Agronomy Vol. 22 No. 2 (1994): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

An experiment consists of two separated trial sets had been carried out at Probolinggo, East Java to study of manual thinning on berry cluster of "BS-6" grape by berry removal and cluster tipping. Berry thinning severities that were investigated consists of berry-removing and cluster tipping, each applied control, 20%, 40% and 60% thinning treatments at 7 or 14 days after anthesis. Berry removal did not influence to berry density but increased large-berry proportion and juice quality; since cluster tipping increased berry density, berry size and berry uniformity. Manual thinning at 7 days after anthesis gave better effect than at 14 days after anthesis

PENGARUH POLUTAN Pb TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TANAMAN Lantana sellowiana dan Ixora chinensis Siti Nurisjah; G. A. Wattimena; Endang Syamsudin; Lily Taslim
Indonesian Journal of Agronomy Vol. 22 No. 1 (1994): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

An experiment on the effect of Pb pollutants on the growth and development of two widely used ornamental plants, i.e. Lantana sellowiana and Ixora chinensis, was conducted at the IPB Greenhouse, Baranangsiang, Bogor from mid September 1990 to the end of December 1990. The experiment aimed to determine the effect of concentration levels of Pb in the form of PbCl2 upon the growth and development of the two ornamental plants. The experiment consisted of two units, each of which was based on the complete randomized design. Treatments consisted of four concentration levels of PbCI2, i.e. 0, 250, 2500, and 25 000 ppm, applied to each unit with three replications. Observation during 12 weeks showed that treatments with 250 and 2500 ppm PbCl2 did not give significant damage to the growth and development of Lantana sellowiana. Concentration level of 25 000 ppm, however, interfered growth and development of this species six weeks after planting. On the other hand, the concentration levels used in this experiment had no toxic effect on the growth and development of Ixora chinensis.

Keragaan dan Hubungan Berbagai Komponen Hasil Tanaman Karet (Hevea brasiliensis Muell. Arg.) pada Dua Populasi Hasil Persilangan PB 260 dengan PN , Novalina; M. Jusuf; G. A. Wattimena; , Suharsono; Aidi Daslin
Indonesian Journal of Agronomy Vol. 36 No. 2 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Latex yield of rubber tree (Hevea brasiliensis Muell. Arg.) is a complex trait influenced by external and internal factors. In this trial, F1 progenies of two populations (crossing PB 260 as high latex yielder x PN as low latex yielder) and their parents were used to study the characteristic of yield components, namely physiology, anatomy and growth characters which influence latex yield. Based on statistic analysis, it showed that latex yield of two populations had high variation among their F1 progenies. The other variables such as girth, bark thickness, number of latex vessel rings, plugging index, sucrose content and inorganic phosphate content also showed high variation. Based on path analysis and stepwise regression, it showed that the number of latex vessel rings and plugging index had bigger direct effect on determining latex yield of A population (PB 260 x PN 7111), while girth and plugging index variables had bigger direct effect on latex yield of C population (PB 260 x PN 7).

Induksi Tanaman Haploid Dianthus sp. melalui Pseudofertilisasi Menggunakan Polen yang Diiradiasi dengan Sinar Gamma , S. Kartikaningrum; , A. Purwito; G. A. Wattimena; B. Marwoto; D. Sukma
Jurnal Hortikultura Indonesia Vol. 4 No. 1 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTHaploid plants of Dianthus sp. were obtained by pseudofertilization using irradiated pollen. Gamma ray of 100 gray was used to inactivate pollen. Crossing were made using 131 female of Dianthus chinensis Dchi-11 as pod parents. After pollination 59% fruits were harvested, 41% were dropped and only 51 ovaries were cultured on the medium. Ovaries were explanted 1 – 2 weeks after pollination and cultured on solid MS medium containing 400 mg L-1glutamine + 103. 77 mg L- 1 proline and growth regulator including 1-Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP). The first ploidy seedling observation was made by number of chloroplast in each side of guard cells of stomata. Four haploid plants were obtained from this screening, but based on chromosome counting and DNA analysis using flow cytometry only three haploid plants and two haploid plants revealed respectively.Key words: BAP, medium, pollination, proline, stomataABSTRAKTanaman haploid Dianthus sp diperoleh melalui pseudofertilisasi menggunakan serbuk sari yang diiradiasi. Sinar gamma dari senyawa cobalt 60Co pada dosis 100 Gy digunakan untuk menonaktifkan serbuk sari. Persilangan semu dilakuan menggunakan 131 bunga betina dari Dianthus chinensis Dchi- 11 sebagai donor ovul. Setelah penyerbukan 41% buah gugur dan hanya 51 ovari dapat dikulturkan pada medium. Ovari ditanam pada umur 1 – 2 minggu setelah penyerbukan dan dikulturkan pada dua media MS yang mengandung 400 mg L-1glutamin + 103. 77 mg L-1 prolin dan dengan penambahan 1-Naphthaleneacetic acid (NAA) dan 6-Benzylaminopurine (BAP). Observasi ploidi awal dilakukan dengan melihat jumlah kloroplas pada sel penjagastomata pada daun. Berdasarkan pengamatan ini empat tanaman haploid diperoleh, namun berdasarkan penghitungan jumlah kromosom hanya tiga tanaman yang haploid, dan berdasarkan an alisis DNA dengan flowcytometer diperoleh dua tanaman haploidKataKunci: BAP, media, penyerbukan, prolin, stomata

PERUBAHAN ANATOMI DAUN PADA REGENERAN MANGGIS AKIBAT IRADIASI SINAR GAMMA IN VITRO Warid Ali Qosim; Roedhy Purwanto; G. A. Wattimena; , Witjaksono
Zuriat Vol 18, No 1 (2007)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v18i1.6745

Tanaman manggis mempunyai laju fotosintesis rendah dan dapat diketahui secara tidak langsung melalui perubahan struktur anatomi daun manggis in vitro. Tujuan penelitian untuk mempelajari perubahan anatomi daun manggis in vitro akibat iradiasi sinar gamma. Sampel daun berasal dari 21 regeneran mutan dan satu kontrol yang sudah diseleksi. Struktur anatomi daun diamati dengan membuat irisan paradermal dan transversal. Irisan paradermal dibuat mengikuti metode sediaan utuh (whole mount) dan diwarnai dengan safranin 1%, sedangkan irisan transversal dibuat dengan mengikuti metode parafin. Daun disayat menggunakan mikrotom putar dengan tebal 10 μm, kemudian diwarnai dengan safranin 1% dan fastgreen 0.5%. Hasil penelitian menunjukkan bahwa iradiasi sinar gamma dapat mempengaruhi perubahan anatomi daun baik pada irisan paradermal maupun transversal. Pada irisan paradermal, luas stomata regeneran mutan pada umumnya lebih besar dibandingkan regeneran kontrol. Regeneran mutan yang memiliki kerapatan dan indeks stomata yang lebih besar dibandingkan kontrol adalah regeneran R-5/2, R-5/3, R-5/4, R-10/4, R-15/3, R-25/1, R-30/1dan R-30/2. Pada irisan transversal, ketebalan kutikula adaksial regeneran mutan lebih tipis dibandingkan kontrol, kecuali regeneran R-5/1. regeneran R- 10/4, R-15/1, R-15/2, R-15/3 memiliki parenkim palisade, bunga karang dan lamina daun yang lebih tebal. Pada umumnya bunga karang dan jumlah berkas pembuluh lebih tebal dibandingkan regeneran kontrol.

Co-Authors , A. Purwito , Novalina , Nurhasanah , S. Kartikaningrum , Samanhudi , Suharsono , Susiyanti , Witjaksono A. A. Mattjik A. Purwito Agus Purwito Aidi Daslin Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R B. Marwoto Citra Bakti D. A. Santosa D. Sukma Ence Darmo Jaya Superna Endang Syamsudin Enny Prasetyanti H . M. Machmud HAJRIAL ASWIDINNOOR Inez H.S. Loeddin Suharsono Lily Taslim M. Jusuf M. Surahman Ni Made Armini Wiendi R. Poerwanto ROEDHY PURWANTO Siti Nurisjah Sri Setyati Harjadi WARID ALI QOSIM Winarso D. Widodo

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