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Nanocurcumin Potential Effect of SOD Enzyme and Caspase-3 Expression in Lead-Acetate Induced Rats Ovarian Granulosa Cells Anis Satus Syarifah; Sri Agus Sudjarwo; Hendy Hendarto; Reny I’tishom; Supriyanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14650

Abstract

Aim: This study investigated the potential effect of nanocurcumin on the increase of SOD enzyme expressionand decrease of caspase-3 in lead acetate-induced rats ovarian granulosa cells.Materials and Methods: Forty five female rats were divided into 5 groups, the negative control group (ratsreceiving corn oil, one hour later receiving distilled water), positive control group (rats receiving corn oil,one hour later receiving lead acetate of 40 mg/kg bw) and experimental groups 1, 2 and 3 (rats receivingnanocurcumin 50 mg, 100 mg and 200 mg/kg bw). One hour after administering the nanocurcumin, therats received 40 mg/kg of lead acetate. All groups received oral treatment once a day for 26 days. Onday 27 the rats were sacrificed and the expression of SOD and caspase-3 enzymes were measured usingimmunohistochemical methods.Results: This study found that lead acetate decreased SOD enzyme expression and increased caspase-3. Incontrast, nanocurcumin increased SOD enzyme and decreased caspase-3 expression in lead acetate-inducedrats ovarian granulosa cells.Conclusion: Nanocurcumin has potential as a strong natural antioxidant by affecting the increase of SODand the decrease of caspase-3 cells in lead acetate-induced rats ovarian granulosa cells.
The Role of Cdk5 and TRPV1 in Meloxicam Resistance Signal Transduction in Rat Experiencing Chronic Pain Mohammad F. Qorib; Reny I’tishom; Sri A. Sudjarwo; Ahmad Basori
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15884

Abstract

Chronic pain is a common case and become a serious problem. Chronic pain management are inconclusive.One of the common treatment option is NSAID. Treatment Failed are around 34-79% of total case. Thesetreatment failed suspected as NSAID resistance. Treatment failed can be caused by a some molecule thatmake NSAID loss of efficacy. Cdk5 is one of the molecule that active in chronic pain condition. Cdk5 canincrease transmembrane insertion and activate TRPV1. The aim of this research is analyzed the role of cdk5and TRPV1 in NSAID resistance of chronic pain rat. This research used 42 rats as a subject and dividedinto 6 groups with random allocation method and factorial design. Meloxicam treatment was given orallyevery day for 7 days after rats have a chronic pain (28 days). Chronic pain induction used a CFA injection.Results : cdk5 and TRPV1 expression at the dorsal root ganglia of chronic pain groups are increase, nosignificant different of pain threshold and inflammation sign between treatment and no treatment groupsafter chronic pain occurred. Conclusion : chronic pain can induce cdk5 and TRPV1 expression, and inducedby meloxicam treatment. Cdk5 and TRPV1 have a positive correlation with meloxicam resistance.
The Correlation of Prostate Volume with Uroflowmetry and International Prostatic Symptoms Score (IPSS) on Patient with Benign Prostatic Hyperplasia without Urinary Retention Arif Rananda; Reny I’tishom; Wahjoe Djatisoesanto; Soetojo
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15613

Abstract

Objective: To value the correlation of Prostate Volume with Uroflowmetry and IPSS on patients with BPHwithout urinary retention that would be conducted at Dr. Soetomo 2015-2017. Method: This research usedretrospective descriptive study using secondary data from medical records to determine the relationshipbetween Prostate Volume (PV) and Uroflowmetry and the International Prostatic Symptom Score (IPSS)of Benign Prostate Hyperplasia (BPH) patients without urinary retention. Uroflowmetry basic parametersis Qmax, Vvoid, and PVR. The sampling technique used the total sampling method that used all existingdata in a certain period. Result: 86 medical records of BPH patients, the age range 45-64 years: 52 patients(60.46%), and the age range> 65 years: 34 patients (39.54%). PV statistical test with Qmax (r = 0.012;p = 0.913), the correlation was not significant. PV with Vvoid (r = 0.112; p = 0.305), the correlation wasnot significant. PV with PVR (r = -0.015; p = 0.892), the correlation was not significant. PV with IPSS (r= 0.048; p = 0.660), the correlation was not significant. Conclusion: There is no correlation on ProstateVolume with Uroflowmetry and IPSS on patient with BPH without urinary retention.
The Potency of Kaffir Lime Leaf (Citrus Hystrix) Ethanol Extract Against Lead Acetate Toxicity on Spermatozoa Quality of Mice (Mus musculus) Azarine Ariqoh Arysanti Bahyshidqi; Safira Nur Izzah; Sofia Zahra Kamila; Reny I’tishom
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14667

Abstract

Lead is a heavy metal which can affect male reproductive system. The accumulation of lead in the bodybecomes a precursor to the formation of ROS, which is able to damage spermatozoa. The toxicity of leadcan be prevented by antioxidants. Kaffir lime leaves (Citrus hystrix) with their flavonoid content have thepotential to be the source of antioxidants. The purpose of this study is to analyze the antioxidant effect ofkaffir lime leaves on the quality of spermatozoa of the mice that have been exposed to lead acetate. Methods:The literature study was conducted using narrative review technique by collecting related articles usingspecified keywords. Result: It is found that kaffir lime leaves contain hesperetin, which is the dominantflavonoid in Citrus group. The antioxidant activity of kaffir lime leaf is able to neutralize lead-induced ROS,as well as to stimulate the formation of enzymatic antioxidants. Conclusions: Ethanol extract of kaffir limeleaves can improve the quality of spermatozoa of the mice that have been exposed to lead acetate.
Increased Corticosterone Levels due to Chronic Stress Reduces IGF-II Expression in Rattus norvigecus Ade Septiari Rahman; Reny I’tishom; Ashon Sa’adi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16827

Abstract

The optimal folliculogenesis process describes a good condition of the female reproductive system.One of the main growth factors in the folliculogenesis is IGF-II which is important for proliferation anddifferentiation of granulosa cells and also steroidogenesis. Chronic stress can cause ovulation failure andfolliculogenesis disorders. Increased corticosterone levels due to chronic stress will cause disruption in the 
THE MUTATION STATUS OF KRAS GENE CODON 12 AND 13 IN COLORECTAL ADENOCARCINOMA (Status Mutasi Gen Kras Kodon 12 dan 13 di Adenocarcinoma Kolorektal) Gondo Mastutik; Alphania Rahniayu; Anny Setijo Rahaju; Nila Kurniasari; Reny I’tishom
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1177

Abstract

Kanker kolorektum merupakan salah satu kanker yang tersering di dunia. Target molekuler untuk pengobatan kanker kolorektumyaitu Epidermal Growth Factor Receptor (EGFR) dengan pemberian antibodi monoklonal anti-EGFR. Pemberian pengobatan ini tidakdapat memberikan efek dampak di pasien dengan status gen KRAS bentuk mutan, sehingga perlu dilakukan pemeriksaan status mutasigen KRAS. Telitian berupa deskriptif dengan pendekatan potong lintang yang bertujuan untuk mendapatkan data status mutasi genKRAS kodon 12 dan 13 di pasien adenocarcinoma colorectal. Deteksi mutasi KRAS dilakukan dengan teknik Polymerase Chain ReactionRestriction Fragment Length Polymorphism (PCR RFLP) yang dikonfirmasi dengan sekuensing. Sampel telitian adalah 30 blok parafinyang diperoleh dari Rumah Sakit Dr.Soetomo Surabaya masa waktu Januari-Desember 2013. Setelah dilakukan ekstraksi DNA terdapat21 sampel yang dapat digunakan untuk pemeriksaan lanjutan. Hasil PCR RFLP menunjukkan terdapat 7/21 mutasi pada kodon12 dan tidak terdapat mutasi gen KRAS pada kodon 13. Mutasi pada kodon 12 yaitu GGT>GCT, GGT>GGA dan GGT>GAT yangmenyebabkan perubahan asam amino Gly12Ala, Gly12Gly dan Gly12Asp. Simpulan telitian ini adalah mutasi gen KRAS kodon 12 padaadenocarcinoma colorectal di Rumah Sakit Dr. Soetomo Surabaya sebanyak 33%.
IN VITRO FERTILITY TEST OF HUMAN SPERMATOZOA MEMBRANE PROTEIN FERTILIN BETA ANTIBODY IN MICE (Mus musculus Balb/c) AS IMMUNOCONTRACEPTIVE CANDIDATE Reny I’tishom; Doddy M Soebadi; Aucky Hinting; Hamdani Lunardhi; Rina Yudiwati
Folia Medica Indonesiana Vol. 52 No. 3 (2016): JULY - SEPTEMBER 2016
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.598 KB) | DOI: 10.20473/fmi.v52i3.5453

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One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.
Mechanism of Corneal Epithelial Cells Death by Infection of pseudomonas Aeruginosathrough Analysis Expression of Caspase-1, TNF, RIPK1, RIPK3, Caspase-3 in Rats Model Ismi Zuhria; Nurwasis; Eddy Bagus Wasito; Winarto; I Ketut Sudiana; Reny I’tishom; Nyilo Purnami
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15802

Abstract

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Detrimental Effect of Alprazolam on Sperm Viability ofMice (Mus musculus) Exposed to Chronic Unpredictable Mild Stress Intan Indah Permatasari; Reny I’tishom; HanikBadriyah Hidayati; Irwanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15805

Abstract

Objective: This study aims to investigate the effect of alprazolam administration on sperm viability of mice (Mus musculus) strain Balb/C following CUMSfor 53 days. Method: This study used a true experimental design through randomization. Control group kept undisturbed without treatment and therapy throughout 60 days. K1 and K2 were exposed to a 53 day period of CUMS along with 7-day pretreatment adaptation. K2 was adapted for a week, stressed for 53 days along with cotreatment of alprazolam (4 mg/kg BW). Results: K1 and K2 showed insignificant improvement of sperm viability (p > 0.05).Meanwhile, K0 and K1 showed significant value compared to K2 (p<0.05). K2 showed little increase of sperm viability in CUMS group along with subsequent treatment of alprazolam. Conclusion: Overall, the results indicate an insignificant effect of alprazolam on sperm viability of CUMS-induced mice.
Ketogenic Diet Slows Down Weight Gain in Juvenile Mus musculus with Benzopyrene as Cancer Inducer Dita Mega Utami; Lilik Herawati; Reny I’tishom; Mohammad Anam Al-Ari; Muhammad Miftahussurur; Purwo Sri Rejeki
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14709

Abstract

Objective: This study aims to analyze the ketogenic diet that slows down weight gain in juvenile Musmusculus induced with benzopyrene (BZP). Method: Thirty-six female mice (Mus musculus), 6-8 weeks old,weighted 15-25 grams were divided into six groups randomly. K1 (negative control, standard diet) inducedwith oleum olivarum, while K2 (positive control, standard diet), P1 (ketogenic diet 1), P2 (ketogenic diet 2),P3 (ketogenic diet 3) and P4 (ketogenic diet 4) induced with BZP subcutaneously in the right breast at a doseof 0.3mg/20gBB/day for 14 days. The ketogenic diet was given for the next 28 days. Data were analyzedusing the ANOVA and post hoc LSD test. Results: After 42 days, the ketogenic diet mice experiencedslower weight gain (p=0.000) because BZP induced neurotoxicity and the administration of the ketogenicdiet in juvenile mice induced neurohormonal changes. The ketogenic diet increased ketone plasma level(p=0.000) and reduced blood glucose levels (p=0.002) due to nutritional ketosis state. Conclusions: Theketogenic diet with fat:protein ratio 60:30 slows down weight gain, increased ketone levels and decreasedblood glucose levels in juvenile mice with BZP as cancer inducer.